Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 431,505 T→C 8.5% S77P (TCA→CCA)  SAS0387 → exotoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX571857431,5050TC 8.5% 938.8 / 12.1 262S77P (TCA→CCA) SAS0387exotoxin
Reads supporting (aligned to +/- strand):  ref base T (110/123);  new base C (13/14);  total (125/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

GCAAATGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGACACAT  >  BX571857/431409‑431601
                                                                                                |                                                                                                
  aaaTGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCa                                                                                                >  1:112197/1‑97 (MQ=255)
     tGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCaaaa                                                                                             <  1:1097076/97‑1 (MQ=255)
     tGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCaaaa                                                                                             >  1:1683375/1‑97 (MQ=255)
       caacaaCACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAAcc                                                                                           <  1:2784969/97‑1 (MQ=255)
         acaacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCaa                                                                                         >  1:1361441/1‑97 (MQ=255)
         acaacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCaa                                                                                         <  1:1558622/97‑1 (MQ=255)
             cacCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCACCACCACAACCAAAcgc                                                                                     >  2:3360693/1‑97 (MQ=17)
              acCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcgcg                                                                                    <  2:2265014/97‑1 (MQ=255)
                cATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAACCACAACCAAACGCGac                                                                                  >  2:1417754/1‑97 (MQ=255)
                  tCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGacaa                                                                                <  1:3180769/97‑1 (MQ=255)
                     tCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAacac                                                                             <  1:3340454/97‑1 (MQ=255)
                       aaCTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCa                                                                           <  1:686764/97‑1 (MQ=255)
                        aCTGAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCGCCGCAATCAAAACCAAACGCGACAACACCAt                                                                          <  2:1078060/97‑1 (MQ=255)
                        aCTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCAt                                                                          <  1:1024100/97‑1 (MQ=255)
                        aCTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCAt                                                                          <  1:2853096/97‑1 (MQ=255)
                          tAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAACCAAAACCAAACACGACAACACCATCt                                                                        >  2:868147/1‑97 (MQ=255)
                           aaaGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATcat                                                                       >  2:2086384/1‑95 (MQ=255)
                           aaaGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCtt                                                                       <  1:3231908/97‑1 (MQ=255)
                           aaaGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCtt                                                                       >  1:2893438/1‑97 (MQ=255)
                            aaGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTc                                                                      <  2:499931/97‑1 (MQ=255)
                             aGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCa                                                                     >  1:2331963/1‑97 (MQ=255)
                              gTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCaa                                                                    >  1:1592654/1‑97 (MQ=255)
                              gTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCaa                                                                    >  1:3109699/1‑97 (MQ=255)
                                ggAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTCCAAca                                                                  >  2:257064/1‑96 (MQ=255)
                                 gAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTa                                                                 >  2:1745205/1‑97 (MQ=255)
                                 gAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTa                                                                 >  2:2949636/1‑97 (MQ=255)
                                  aaGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTaa                                                                <  1:224737/97‑1 (MQ=255)
                                  aaGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTaa                                                                >  1:2547732/1‑97 (MQ=255)
                                   aGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTaaa                                                               >  2:528218/1‑97 (MQ=255)
                                    gCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAg                                                              <  2:2219887/97‑1 (MQ=255)
                                     cACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGt                                                             >  1:750722/1‑97 (MQ=255)
                                     cACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGt                                                             >  1:2228453/1‑97 (MQ=255)
                                      aCCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTg                                                            >  1:3471200/1‑97 (MQ=255)
                                      aCCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTg                                                            >  2:1823570/1‑97 (MQ=255)
                                      aCCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAAACAAAACCAAACGCGACAACACCATCTTCAACAAAAGta                                                            >  2:3036952/1‑96 (MQ=255)
                                       ccGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTgg                                                           <  2:651832/97‑1 (MQ=255)
                                       ccGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTgg                                                           <  1:1723436/97‑1 (MQ=255)
                                       ccGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTgg                                                           <  1:393232/97‑1 (MQ=255)
                                         gCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGaa                                                         >  2:3551665/1‑97 (MQ=255)
                                           aacaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGc                                                       >  1:280486/1‑97 (MQ=255)
                                              aaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCAcc                                                    >  2:1329823/1‑97 (MQ=255)
                                               agcagcAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCg                                                   >  1:23813/1‑97 (MQ=255)
                                               agcagcAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCg                                                   >  1:510696/1‑97 (MQ=255)
                                                 cagcaAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGca                                                 >  2:3454437/1‑97 (MQ=255)
                                                  agcaAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaa                                                >  1:144977/1‑97 (MQ=255)
                                                   gcaAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaac                                               <  2:742743/97‑1 (MQ=255)
                                                    caAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaaca                                              <  2:163117/97‑1 (MQ=255)
                                                     aaaCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAACGTGGAAGCACCGcaacaa                                             >  1:2639709/1‑97 (MQ=255)
                                                      aaCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAag                                            <  2:825366/97‑1 (MQ=255)
                                                      aaCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAag                                            <  1:214266/97‑1 (MQ=255)
                                                       aCGCGACAACACCGTCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAagc                                           <  2:3182605/97‑1 (MQ=255)
                                                       aCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAagc                                           <  1:3365340/97‑1 (MQ=255)
                                                       aCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAagc                                           <  2:571835/97‑1 (MQ=255)
                                                        cgcgACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAGCGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAagca                                          <  1:1984529/97‑1 (MQ=255)
                                                            acaacaCCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCaa                                      >  2:1165375/1‑97 (MQ=255)
                                                              aacaCCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAACAAGCAGCAAAc                                    >  2:963371/1‑97 (MQ=255)
                                                               acacCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcg                                   >  1:17924/1‑97 (MQ=255)
                                                               acacCATCGTCAACTAACGTAGAAGCACCGCAATCATAACCAAACGCGACAACACAATCTTCAACTAAAATGGAAGCATCGCAACACGCAGCAAAcg                                   <  2:1270148/97‑1 (MQ=18)
                                                                cacCATCTTCAACTAAATTAGAAGCACCGTAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcgc                                  >  2:2765172/1‑97 (MQ=255)
                                                                cacCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcgc                                  <  1:2826877/97‑1 (MQ=255)
                                                                 acCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcgcg                                 >  2:1182330/1‑97 (MQ=255)
                                                                 acCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcgcg                                 >  2:2396833/1‑97 (MQ=255)
                                                                   caccTTCAACTAAAGTAGAAGCACCGCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGac                               <  2:3493960/94‑1 (MQ=255)
                                                                   cATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGac                               <  1:580297/97‑1 (MQ=255)
                                                                    aTCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGaca                              >  2:372063/1‑97 (MQ=255)
                                                                    aTCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGaca                              <  1:331964/97‑1 (MQ=255)
                                                                    aTCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGaca                              >  2:328167/1‑97 (MQ=255)
                                                                     tCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaa                             <  1:1817359/97‑1 (MQ=255)
                                                                     tCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaa                             >  1:3393480/1‑97 (MQ=255)
                                                                       ttCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaaca                           >  2:1962031/1‑97 (MQ=255)
                                                                        tCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAacac                          <  1:131298/97‑1 (MQ=255)
                                                                          aaCTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcacca                        >  2:2005464/1‑97 (MQ=255)
                                                                           aCTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcaccac                       <  1:1221697/97‑1 (MQ=255)
                                                                            cTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACaccacc                      <  1:3372327/97‑1 (MQ=255)
                                                                            cTAAAGTAGAAGCACCGCAACCAAGACCAAACGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACaccacc                      <  2:1582541/97‑1 (MQ=255)
                                                                             tAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCt                     >  1:1532328/1‑97 (MQ=255)
                                                                               aaGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTc                   <  1:2599003/97‑1 (MQ=255)
                                                                                aGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCg                  <  1:2083170/97‑1 (MQ=255)
                                                                                 gTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGt                 >  2:2883238/1‑97 (MQ=255)
                                                                                 gTAGAAGCACCGCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGt                 <  2:275390/97‑1 (MQ=255)
                                                                                  tAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTc                >  1:965849/1‑97 (MQ=255)
                                                                                  tAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTc                >  2:3001894/1‑97 (MQ=255)
                                                                                   aGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAACACCGCAACAAGCAGAAAACGCGACAACACCACCTTCGTca               >  2:2451297/1‑96 (MQ=255)
                                                                                    gAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTCCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGCCTa              >  1:1720630/1‑97 (MQ=255)
                                                                                     aaGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTaa             >  2:798673/1‑97 (MQ=255)
                                                                                     aaGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTaa             >  2:2837928/1‑97 (MQ=255)
                                                                                     aaGCACCGCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTaa             <  2:1650062/97‑1 (MQ=255)
                                                                                      aGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAAt            >  2:2212189/1‑97 (MQ=255)
                                                                                      aGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAAt            >  1:3016446/1‑97 (MQ=255)
                                                                                        cACCGCAATCAAAACCAAACGCGGCAACACCATCTTCAACTAAGGTGGAGGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGt          >  1:2189194/1‑97 (MQ=255)
                                                                                        cACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGt          >  1:851497/1‑97 (MQ=255)
                                                                                         aCCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTa         <  1:2521810/97‑1 (MQ=255)
                                                                                         aCCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTa         >  2:230837/1‑97 (MQ=255)
                                                                                           cGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTatg       <  1:451997/97‑3 (MQ=255)
                                                                                            gCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGac      >  1:3157609/1‑97 (MQ=255)
                                                                                             cAATCAAAACCAAACGCGACAACACCATCGTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGaca     <  1:1383434/97‑1 (MQ=255)
                                                                                              aaTCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGacac    <  1:1432213/97‑1 (MQ=255)
                                                                                               aTCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTCATGTAGAcaca   >  2:701207/1‑97 (MQ=255)
                                                                                               aTCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGAcaca   >  1:2015692/1‑97 (MQ=255)
                                                                                                tCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGACACAt  <  1:1637383/97‑1 (MQ=255)
                                                                                                |                                                                                                
GCAAATGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGACACAT  >  BX571857/431409‑431601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑