BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BX571857	2,379,210	G→T	9.5%	A51E (GCA→GAA)	SAS2224 ←	urocanate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BX571857	2,379,210	0	G	T	9.5%	837.8 / 52.0	207	A51E (GCA→GAA)	SAS2224	urocanate hydratase
Reads supporting (aligned to +/- strand): ref base G (89/98); new base T (7/13); total (96/111)
Fisher's exact test for biased strand distribution p-value = 3.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 227 total aligned reads displayed.

ACAGCAACTGGCTTTCCTGATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGTATAACATTCTAAGTACTGCTTCTT  >  BX571857/2379114‑2379306

aCAGCAACTGGCTTTCCTGATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGtg                                                                                                  <  1:2862764/97‑1 (MQ=255)

  ggCAACTGGCTTTCCTGATTGTACTACCATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCGAATTACGtgct                                                                                                <  2:1311191/96‑1 (MQ=255)

  aGCAACTGGCTTTCCTGATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGtgct                                                                                                <  2:1160502/97‑1 (MQ=255)

  aGCAACTGGCTTTCCTGATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGtgct                                                                                                <  1:2154935/97‑1 (MQ=255)

   gCAACTGGCTTTCCTGATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGtgctg                                                                                               >  2:2368110/1‑97 (MQ=255)

   gCAACTGGCTTTCCTGATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGtgctg                                                                                               >  2:3209886/1‑97 (MQ=255)

    cAACTGGCTTTCCTGATTGTACTAACTTTGTCTCATCTGATTCTAATTCTCGTAACGTTTCCTCCATTGCTTCAAAAGCTTCCCAATTACGtgctgc                                                                                              >  1:1031598/1‑97 (MQ=255)

     aaCTGGCTTTCCTGATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTgctgct                                                                                             <  2:758452/97‑1 (MQ=255)

      aCTGGCTTTCCTGATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCtt                                                                                            <  2:1235677/97‑1 (MQ=255)

          gCTTTCCTGATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTCACGTTTTCTCTATTGCTTCTCAAGCTTCCCTATTACGTGCTGCTTTTcc                                                                                        >  2:26205/1‑97 (MQ=255)

          gCTTTCCTGATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTcc                                                                                        >  2:2882788/1‑97 (MQ=255)

            tttCCTGATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCaa                                                                                      <  1:229031/97‑1 (MQ=255)

             ttCCTGATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAAt                                                                                     <  1:2742313/97‑1 (MQ=255)

             ttCCTGATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAAt                                                                                     >  1:3329123/1‑97 (MQ=255)

             ttCCTGATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAAt                                                                                     <  2:2740841/97‑1 (MQ=255)

              tCCTGATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATa                                                                                    >  1:1515624/1‑97 (MQ=255)

              tCCTGATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATa                                                                                    >  2:132941/1‑97 (MQ=255)

              tCCTGATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATa                                                                                    >  2:1718709/1‑97 (MQ=255)

                cTGATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATacc                                                                                  <  2:1988845/97‑1 (MQ=255)

                cTGATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATacc                                                                                  <  2:2273355/97‑1 (MQ=255)

                 tGATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATacca                                                                                 >  2:416572/1‑97 (MQ=255)

                  gATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTTCTGCTTTTCCAATaccac                                                                                <  1:566302/97‑1 (MQ=255)

                    ttGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATAccacca                                                                              >  1:1413446/1‑97 (MQ=255)

                     tGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCAt                                                                             >  2:2660989/1‑97 (MQ=255)

                      gTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATa                                                                            >  1:2303946/1‑97 (MQ=255)

                        aCTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTTCTGCTTTTCCAATACCACCATaaa                                                                          <  2:1746140/97‑1 (MQ=255)

                         cTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAaac                                                                         <  1:3170850/97‑1 (MQ=255)

                         cTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAaac                                                                         >  1:391058/1‑97 (MQ=255)

                         cTAACACTGTCTCATCTGAGTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCGTAaac                                                                         <  2:370041/97‑1 (MQ=255)

                          tAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAaaca                                                                        >  1:2419817/1‑97 (MQ=255)

                           aaCATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTTCTGCTTTTCCAATACCACCATAaacaa                                                                       >  2:897167/1‑97 (MQ=255)

                            aCATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAaacaac                                                                      >  2:1934417/1‑97 (MQ=255)

                              aTTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTa                                                                    >  1:2174857/1‑97 (MQ=255)

                              aTTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTa                                                                    <  1:2808130/97‑1 (MQ=255)

                               ttGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTTCTGCTTTTCCAATACCACCATAAACAACTaa                                                                   <  1:1737817/97‑1 (MQ=255)

                               ttGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTaa                                                                   >  2:2421147/1‑97 (MQ=255)

                                tGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTaaa                                                                  <  1:1141064/97‑1 (MQ=255)

                                 gTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAAt                                                                 >  1:1492464/1‑97 (MQ=255)

                                 gTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAAt                                                                 >  1:471175/1‑97 (MQ=255)

                                 gTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAAt                                                                 >  1:495770/1‑97 (MQ=255)

                                 gTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAAt                                                                 <  1:868897/97‑1 (MQ=255)

                                  tctcATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAAtc                                                                <  1:2624192/97‑1 (MQ=255)

                                   ctcATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAAtct                                                               >  1:1845149/1‑97 (MQ=255)

                                        cTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTgg                                                          >  2:544097/1‑97 (MQ=255)

                                         tGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGt                                                         >  2:3306680/1‑97 (MQ=255)

                                           aTTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCt                                                       >  1:3272591/1‑97 (MQ=255)

                                            ttCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCtt                                                      <  2:2183385/97‑1 (MQ=255)

                                              cTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTc                                                    >  2:3143646/1‑97 (MQ=255)

                                              cTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTc                                                    >  2:610767/1‑97 (MQ=255)

                                              cTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTc                                                    >  1:605514/1‑97 (MQ=255)

                                                  ttCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCa                                                <  1:1332686/97‑1 (MQ=255)

                                                  ttCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCa                                                <  2:1452080/97‑1 (MQ=255)

                                                   tctcGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCaa                                               <  2:245750/97‑1 (MQ=255)

                                                   tctcGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCaa                                               >  1:2751319/1‑97 (MQ=255)

                                                   tctcGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCaa                                               <  1:1240866/97‑1 (MQ=255)

                                                   tctcGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCaa                                               >  1:136886/1‑97 (MQ=255)

                                                   tctcGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCaa                                               >  2:1088452/1‑97 (MQ=255)

                                                    ctcGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAAc                                              <  2:1788756/97‑1 (MQ=255)

                                                    ctcGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAAc                                              <  2:209099/97‑1 (MQ=255)

                                                     tcGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACt                                             <  2:1380693/97‑1 (MQ=255)

                                                     tcGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACt                                             <  1:1279114/97‑1 (MQ=255)

                                                       gTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTc                                           <  1:2651381/97‑1 (MQ=255)

                                                       gTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCCGCTTTTCCAATACCACCTTAAACACCTAAATCTTCTTGTCTTTCAGCAACTTc                                           >  2:1423218/1‑97 (MQ=255)

                                                         aaCGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTg                                         <  1:3353252/97‑1 (MQ=255)

                                                           cGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTggg                                       <  1:934133/97‑1 (MQ=255)

                                                           cGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTggg                                       >  1:309413/1‑97 (MQ=255)

                                                           cGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTggg                                       <  1:2231611/97‑1 (MQ=255)

                                                            gTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGt                                      >  1:2909791/1‑97 (MQ=255)

                                                               ttCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTa                                   <  1:176673/97‑1 (MQ=255)

                                                                tctcTATTGCTTCAAAAGCTTCCCAATTACGTTCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTaa                                  <  2:96546/97‑1 (MQ=255)

                                                                   ctATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAAtt                               >  1:1012537/1‑97 (MQ=255)

                                                                   ctATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAAtt                               <  1:2819458/97‑1 (MQ=255)

                                                                     aTTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAAttgt                             <  1:2592211/97‑1 (MQ=255)

                                                                      ttGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAAttgtt                            <  1:1311827/97‑1 (MQ=255)

                                                                      ttGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAAttgtt                            <  2:1804452/97‑1 (MQ=255)

                                                                      ttGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAAttgtt                            >  1:886933/1‑97 (MQ=255)

                                                                       tGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAAttgttg                           <  1:2886835/97‑1 (MQ=255)

                                                                         cTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGta                         >  1:1823406/1‑97 (MQ=255)

                                                                          ttCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGtat                        <  1:1209185/97‑1 (MQ=255)

                                                                          ttCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGtat                        <  1:159766/97‑1 (MQ=255)

                                                                           tCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGtata                       >  2:2913978/1‑97 (MQ=255)

                                                                            cAAAAGCTTCCCAATTACGTTCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGTATaa                      <  1:796776/97‑1 (MQ=255)

                                                                               aaGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCGGGGTCTAAATTGTTGTATAACAt                   <  1:902837/97‑1 (MQ=255)

                                                                                 gCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGTATAACATTc                 <  1:858210/97‑1 (MQ=255)

                                                                                 gCTTCCCAATTACGTGCTGCTTTTCCAATACCACCAGAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGTATAACATTc                 <  1:2074475/97‑1 (MQ=255)

                                                                                  cTTCCCTATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGTATAACATTCt                <  1:3147936/97‑1 (MQ=255)

                                                                                  cTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGTATAACATTCt                >  2:1262714/1‑97 (MQ=255)

                                                                                    tCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGTATAACATTCTaa              >  2:1543580/1‑97 (MQ=255)

                                                                                     cccAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGTATAACATTCTAAg             <  1:1064289/97‑1 (MQ=255)

                                                                                     cccAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGTATAACATTCTAAg             >  1:2485488/1‑97 (MQ=255)

                                                                                      ccAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGTATAACATTCTAAGt            <  2:1134235/97‑1 (MQ=255)

                                                                                         aTTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGTATAACATTCTAAGTACt         <  2:326414/97‑1 (MQ=255)

                                                                                          ttACGTTCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGTATAACATTCTAAGTACTg        >  1:1966144/1‑97 (MQ=255)

                                                                                          ttACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGTATAACATTCTAAGTACTg        <  2:3128651/97‑1 (MQ=255)

                                                                                           tACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGTATAACATTCTAAGTACTGc       >  1:1428703/1‑97 (MQ=255)

                                                                                            aCGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGTATAACATTCTAAGTACTGct      <  1:949644/97‑1 (MQ=255)

                                                                                            aCGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGACTTTCAGCAACTTCTGGGTCTAAATTGTTGTATAACATTCTAAGTACTGct      >  2:2572146/1‑97 (MQ=255)

                                                                                            aCGTGCTGCTTTTCCAAGACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGTATAACATTCTAAGTACTGct      <  2:2065253/97‑1 (MQ=255)

                                                                                              gTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGTATAACATTCTAAGTACTGcttc    <  2:3166175/97‑1 (MQ=255)

                                                                                                gctgctTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGTATAACATTCTAAGTACTGcttctt  <  2:833301/97‑1 (MQ=255)

ACAGCAACTGGCTTTCCTGATTGTACTAACATTGTCTCATCTGATTCTAATTCTCGTAACGTTTTCTCTATTGCTTCAAAAGCTTCCCAATTACGTGCTGCTTTTCCAATACCACCATAAACAACTAAATCTTCTGGTCTTTCAGCAACTTCTGGGTCTAAATTGTTGTATAACATTCTAAGTACTGCTTCTT  >  BX571857/2379114‑2379306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`