BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BX571857	1,252,438	T→C	100%	intergenic (+358/‑77)	SAS1171 → / → SAS1172	signal recognition particle protein/30S ribosomal protein S16

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BX571857	1,252,438	0	T	C	99.7%	734.6 / ‑6.1	169	intergenic (+358/‑77)	SAS1171/SAS1172	signal recognition particle protein/30S ribosomal protein S16
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (80/88); minor base G (1/0); total (81/88)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 169 total aligned reads displayed.

TTATAACCTATTAACAGTCTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTATAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGATTTTATAAATGGCAGTTAAAATTCG  >  BX571857/1252345‑1252531

  ataACCTATTAACAGTCTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACa                                                                                          <  1:30659/97‑1 (MQ=255)

    aaCCTATTAACAGTCTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACAtt                                                                                        <  2:1176052/97‑1 (MQ=255)

    aaCCTATTAACAGTCTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACAtt                                                                                        <  2:2050595/97‑1 (MQ=255)

    aaCCTATTAACAGTCTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACAtt                                                                                        <  2:3318885/97‑1 (MQ=255)

     aCCTATTAACAGTCTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTc                                                                                       >  2:2395735/1‑97 (MQ=255)

      ccTATTAACAGTCTAATTTAGTAATCACGTAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAGCTCTTTACAAACATTca                                                                                      <  2:1704224/97‑1 (MQ=255)

      ccTATTAACAGTCTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTca                                                                                      >  1:1399365/1‑97 (MQ=255)

       cTATTAACAGTCTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTcac                                                                                     <  1:2278737/97‑1 (MQ=255)

        tcttAACAGTCTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTcaca                                                                                    >  2:35682/3‑97 (MQ=255)

        tATTAACAGTCTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTcaca                                                                                    <  1:2094868/97‑1 (MQ=255)

          ttAACAGTCTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTcacaca                                                                                  >  1:1364208/1‑97 (MQ=255)

           tAACAGTCTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCacacac                                                                                 <  2:2955306/97‑1 (MQ=255)

           tAACAGTCTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCacacac                                                                                 <  1:2610183/97‑1 (MQ=255)

            aaCAGTCTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACAcc                                                                                <  2:602040/97‑1 (MQ=255)

             aCAGTCTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCt                                                                               <  2:2680204/97‑1 (MQ=255)

                gTCTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGtt                                                                            >  2:2985038/1‑97 (MQ=255)

                  cTAATTTATTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTaa                                                                          <  2:255414/97‑1 (MQ=255)

                  cTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTaa                                                                          >  1:2798271/1‑97 (MQ=255)

                  cTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTaa                                                                          <  1:901016/97‑1 (MQ=255)

                  cTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTaa                                                                          <  1:3327555/97‑1 (MQ=255)

                  cTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTaa                                                                          <  1:2508610/97‑1 (MQ=255)

                  cTAATTTAGTAATCACATAATTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGGTaa                                                                          >  2:486839/1‑97 (MQ=255)

                   tAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAat                                                                         <  1:2495808/97‑1 (MQ=255)

                   tAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAat                                                                         <  1:1213551/97‑1 (MQ=255)

                     aTTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAatat                                                                       <  1:2057304/97‑1 (MQ=255)

                     aTTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAatat                                                                       <  2:2871449/97‑1 (MQ=255)

                     aTTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAatat                                                                       <  1:2540191/97‑1 (MQ=255)

                        tAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAAtattat                                                                    <  2:57866/97‑1 (MQ=255)

                         aGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATattatt                                                                   <  1:2240486/97‑1 (MQ=255)

                         aGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATattatt                                                                   <  2:135109/97‑1 (MQ=255)

                           tAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTc                                                                 <  1:2303151/97‑1 (MQ=255)

                           tAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTc                                                                 <  1:3319865/97‑1 (MQ=255)

                            aaTCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCt                                                                <  2:1598928/97‑1 (MQ=255)

                             aTCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCtt                                                               <  1:3258432/97‑1 (MQ=255)

                             aTCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCtt                                                               <  1:1108773/97‑1 (MQ=255)

                             aTCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCtt                                                               >  2:1256342/1‑97 (MQ=255)

                              tCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTg                                                              <  2:1527018/97‑1 (MQ=255)

                              tCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTg                                                              <  2:2012245/97‑1 (MQ=255)

                                acaTAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTa                                                            <  1:73127/97‑1 (MQ=255)

                                 caTAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAg                                                           >  1:1254357/1‑97 (MQ=255)

                                  aTAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGa                                                          >  1:3200726/1‑97 (MQ=255)

                                   tAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGaa                                                         <  1:2978891/97‑1 (MQ=255)

                                   tAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGaa                                                         >  1:306415/1‑97 (MQ=255)

                                   tAGCTATTATTGTAAGAAAAAGGTTCAATAAGTTTCCTGGTAAAGAAATAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGaa                                                         <  1:83291/97‑1 (MQ=255)

                                    aGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGaaa                                                        >  1:535064/1‑97 (MQ=255)

                                       tattatTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATa                                                     >  2:1294421/1‑97 (MQ=255)

                                         ttattGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATaaa                                                   >  1:544968/1‑97 (MQ=255)

                                          tattGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACTCCTGTTAATATTATTTCTTGTAGAAAATaaaa                                                  <  2:1871273/97‑1 (MQ=255)

                                           attGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATaaaaa                                                 >  2:931133/1‑97 (MQ=255)

                                            ttGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAAt                                                <  1:1101708/97‑1 (MQ=255)

                                              gTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTa                                              >  1:1827886/1‑97 (MQ=255)

                                               tAAGAAAAAGGTTCAATACTTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTaa                                             <  1:1469596/97‑1 (MQ=255)

                                                aaGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTaaa                                            >  1:1151649/1‑97 (MQ=255)

                                                 aGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTaaaa                                           <  1:210286/97‑1 (MQ=255)

                                                 aGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTaaaa                                           <  1:2673116/97‑1 (MQ=255)

                                                 aGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTaaaa                                           <  1:273998/97‑1 (MQ=255)

                                                  gAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAAc                                          <  1:2722048/97‑1 (MQ=255)

                                                   aaaaaGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACa                                         <  1:3271822/97‑1 (MQ=255)

                                                   aaaaaGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACa                                         >  2:282763/1‑97 (MQ=255)

                                                    aaaaGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACAt                                        >  1:687557/1‑97 (MQ=255)

                                                     aaaGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATg                                       <  1:1622171/97‑1 (MQ=255)

                                                     aaaGGTTCAATAATTTTCCTGGTAAAGAAAAAACTATTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACAtc                                       >  2:2751180/1‑96 (MQ=255)

                                                      aaGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGa                                      >  2:450994/1‑97 (MQ=255)

                                                       aGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGAc                                     >  2:3080096/1‑97 (MQ=255)

                                                        ggTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACt                                    >  1:2858049/1‑97 (MQ=255)

                                                         gTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACtt                                   >  2:2908952/1‑97 (MQ=255)

                                                         gTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACtt                                   >  2:255759/1‑97 (MQ=255)

                                                          ttCAATAATTTTCCTGGTAAAGAGAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATCAAAATTAAAACATGACTTa                                  >  2:3011798/1‑97 (MQ=255)

                                                          ttCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTa                                  >  1:1213092/1‑97 (MQ=255)

                                                          ttCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTa                                  <  2:2333543/97‑1 (MQ=255)

                                                           tCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTaa                                 >  2:1554505/1‑97 (MQ=255)

                                                            cAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAATTAAAAATTAAAACATGACTTaaa                                >  2:1820333/1‑97 (MQ=255)

                                                              ataatTTTCCTGATAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAgg                              <  2:2652710/97‑1 (MQ=255)

                                                               taatTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGga                             >  1:533958/1‑97 (MQ=255)

                                                                 atTTTCCTGGTAAAGAATAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGgaga                           <  1:1254469/97‑1 (MQ=255)

                                                                 atTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGgaga                           <  1:3155764/97‑1 (MQ=255)

                                                                 atTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGgaga                           <  1:2517895/97‑1 (MQ=255)

                                                                    ttCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGAttt                        >  1:975501/1‑97 (MQ=255)

                                                                     tCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGAtttt                       <  1:2375777/97‑1 (MQ=255)

                                                                          gTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGATTTTATaaa                  >  2:2854487/1‑97 (MQ=255)

                                                                          gTAAAGAAAAAACTCTTTACAAACATCAAAACACCTGTTAATATTATTTCAGGTAGAACTTAAGAATTATAACATGCCTTAAAGGAGATTTTATaaa                  >  2:3275437/1‑97 (MQ=255)

                                                                            aaaGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGATTTTATAAATg                >  1:644492/1‑97 (MQ=255)

                                                                            aaaGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGATTTTATAAATg                >  1:1802416/1‑97 (MQ=255)

                                                                             aaGAAAAAACTCTTTACAAACATTCACAAACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGATTTTATAAATgg               <  1:700287/97‑1 (MQ=255)

                                                                             aaGAAAAAACTCTGTACAAACATGCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGATTTTATAAATgg               <  2:2551508/97‑1 (MQ=255)

                                                                               gAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCCTGTAGAAAATAAAAATTAAAACAGGACTTAAAGGAGATTTTATAAATGGCa             >  1:2064050/1‑97 (MQ=255)

                                                                                 aaaaaCTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGATTTTATAAATGGCAGt           >  1:2946967/1‑97 (MQ=255)

                                                                                 aaaaaCTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGATTTTATAAATGGCAGt           >  2:501062/1‑97 (MQ=255)

                                                                                  aaaaCTCTTTACAAACATTCACACACTTGTTAATATTATTTTGTGTAGAAAATAAAAATTAAAACATGACTTCAAGGAGTTTTTATAAATGGCAGtt          >  2:1910401/1‑97 (MQ=255)

                                                                                  aaaaCTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGATTTTATAAATGGCAGtt          >  1:3310278/1‑97 (MQ=255)

                                                                                   aaaCTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGATTTTATAAATGGCAGTTa         >  1:1226439/1‑97 (MQ=255)

                                                                                   aaaCTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTCAAAGGAGATTTTATAAATGGCAGTTa         >  2:885081/1‑97 (MQ=255)

                                                                                    aaCTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGATTTTATAAATGGCAGTTaa        >  1:2933556/1‑97 (MQ=255)

                                                                                    aaCTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGATTTTATAAATGGCAGTTaa        <  1:2753226/97‑1 (MQ=255)

                                                                                      ctctTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGATTTTATAAATGGCAGTTaaaa      <  2:1101418/97‑1 (MQ=255)

                                                                                      ctctTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGATTTTATAAATGGCAGTTaaaa      >  1:723723/1‑97 (MQ=255)

                                                                                      ctctTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGATTTTATAAATGGCAGTTaaaa      <  2:1988954/97‑1 (MQ=255)

                                                                                        ctTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGATTTTATAAATGGCAGTTAAAAtt    >  1:2436549/1‑97 (MQ=255)

                                                                                         tttACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGATTTTATAAATGGCAGTTAAAATTc   <  1:2440621/97‑1 (MQ=255)

                                                                                          ttACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGATTTTATAAATGGCAGTTAAAATTCg  <  1:3189747/97‑1 (MQ=255)

TTATAACCTATTAACAGTCTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTATAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGATTTTATAAATGGCAGTTAAAATTCG  >  BX571857/1252345‑1252531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`