Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 2,700,131 A→G 6.6% D779D (GAT→GAC SAS2516 ← fibrinogen and keratin‑10 binding surface anchored protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX5718572,700,1310AG 6.6% 766.6 / 4.5 223D779D (GAT→GACSAS2516fibrinogen and keratin‑10 binding surface anchored protein
Reads supporting (aligned to +/- strand):  ref base A (92/108);  new base G (13/5);  total (107/116)
Fisher's exact test for biased strand distribution p-value = 4.71e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 259 total aligned reads displayed.

ATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAA  >  BX571857/2700035‑2700227
                                                                                                |                                                                                                
atcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTatctgaa                                                                                                  <  2:1767304/97‑1 (MQ=255)
atcGCTGTCGGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTTAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTatctgaa                                                                                                  <  2:1748620/97‑1 (MQ=255)
 tcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTatctgaat                                                                                                 >  1:1018879/1‑97 (MQ=255)
     tGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTatctgaatctga                                                                                             <  1:508608/97‑1 (MQ=255)
     tGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTatctgaatctga                                                                                             <  1:804383/97‑1 (MQ=255)
      gtctgagtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGGTAtctgaatctgaa                                                                                            >  1:1492058/1‑97 (MQ=255)
         tgagtctgaATCGCTTTCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGATTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCg                                                                                         <  2:159091/97‑1 (MQ=255)
         tgagtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCTCAGTCTGAGTCTGAGTCTGAATCTGAGTCg                                                                                         >  2:2592234/1‑97 (MQ=11)
         tgagtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGGTGTCTGAGTCTGAGTCGGTATCTGAGTCCGAGTCg                                                                                         >  2:1261519/1‑97 (MQ=18)
           agtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGGTGTCTGGGTCTGAGTCGGGAGCTGAGTCTGAATCggt                                                                                       >  2:2012900/1‑95 (MQ=18)
           agtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCt                                                                                       >  2:860413/1‑97 (MQ=255)
           agtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGcg                                                                                       >  1:1813736/1‑96 (MQ=255)
            gtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCAGTCGGAGTCTGAGTCGCTATCGGAATCTGAATCGcag                                                                                      >  2:22743/1‑95 (MQ=21)
              ctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCAGCCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTgtc                                                                                    >  1:1708346/1‑97 (MQ=255)
               tgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTgtct                                                                                   <  2:2498425/97‑1 (MQ=255)
               tgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCGATCTGAATCGATGTCCGAGACTGAGCCGCTACCTGAGTGTGAATCCCCgtct                                                                                   >  2:2050942/1‑97 (MQ=18)
                gaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTgtctg                                                                                  <  1:2851391/97‑1 (MQ=255)
                gaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTgtctg                                                                                  <  2:2702127/97‑1 (MQ=255)
                 aaTCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTgtctga                                                                                 <  1:1480556/97‑1 (MQ=255)
                 aaTCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTgtctga                                                                                 >  1:1773282/1‑97 (MQ=255)
                   tCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTgtctgagt                                                                               >  1:1076838/1‑97 (MQ=255)
                   tCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTgtctgagt                                                                               <  1:2614634/97‑1 (MQ=255)
                   tCGCTATCGGCTTGTGAGTCGCTGGCTGAGTCTGTGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTgtctgagt                                                                               <  2:1402818/97‑1 (MQ=18)
                    cGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCGCTGTCTGTGTCTGAGTCGCAGTCTGAGTCTGAGTCTGTgtctgggtc                                                                              >  2:469630/1‑97 (MQ=17)
                     gCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTgtctgagtct                                                                             >  2:1893773/1‑97 (MQ=255)
                     gCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTgtctgagtct                                                                             >  1:1284595/1‑97 (MQ=255)
                      ctctCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTgtctgagtctg                                                                            <  2:3013340/94‑1 (MQ=255)
                      cTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTgtctgagtctg                                                                            <  2:2260436/97‑1 (MQ=255)
                      cTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTgtctgagtctg                                                                            <  1:2769932/97‑1 (MQ=255)
                       tATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTgtctgagtctga                                                                           <  1:673622/97‑1 (MQ=255)
                       tATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTgtctgagtctga                                                                           <  1:597507/97‑1 (MQ=255)
                        ttCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGaa                                                                          <  1:1868347/96‑1 (MQ=255)
                        aTCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGaa                                                                          <  1:2291927/97‑1 (MQ=255)
                         tCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAAt                                                                         >  1:1884863/1‑97 (MQ=255)
                         tCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAAt                                                                         >  1:431385/1‑97 (MQ=255)
                            ggttCGGAGGCGGTGGCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCGGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGc                                                                      <  2:2349616/95‑1 (MQ=18)
                             gttCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCt                                                                     <  2:2243710/96‑1 (MQ=255)
                             aTTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGGTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCt                                                                     >  2:735032/1‑97 (MQ=255)
                             aTTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCt                                                                     <  2:1092914/97‑1 (MQ=255)
                              ttCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTa                                                                    <  1:2201992/97‑1 (MQ=255)
                               tCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGACTCTGAATCGCTGTCTGAGTCTGAATCGCtgt                                                                   >  1:1797178/1‑95 (MQ=255)
                                cTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCAGTCTGTGTCTGAGTCGGAATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCGATc                                                                  >  2:1547463/1‑97 (MQ=18)
                                 cgAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCt                                                                 <  2:713730/96‑1 (MQ=255)
                                   aGTCGCTGTCTGAGTCTGAGTCGCTATCTGCATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCGGAGTCGCTATCTGa                                                               >  2:1940286/1‑97 (MQ=255)
                                    gTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGag                                                              >  2:1711312/1‑96 (MQ=255)
                                      cGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGGTATCTGAGTCTGAACCGCGGTCCGAGTCTGAAGCGCTATCTGacgc                                                            >  2:610502/1‑94 (MQ=18)
                                       gCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtct                                                           >  1:1651783/1‑97 (MQ=255)
                                         tGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGCGTCGCTGTCTGAGTCTGAATCGCTATCTGAGtctgg                                                         >  2:2059043/1‑96 (MQ=17)
                                         tGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctga                                                         <  1:451417/97‑1 (MQ=255)
                                          gtctgagtctgaGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGACTCGCTGTCTGAGTCTGAATCGCTAtctgagtctgag                                                        >  2:669519/1‑97 (MQ=255)
                                            ctgagtctgtatCGGTATCTGACTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAAtctgagtc                                                      <  2:1040935/97‑1 (MQ=14)
                                            cggagtctgagtcGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTAGCTGAGTCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtc                                                      <  2:1855270/95‑1 (MQ=255)
                                             tgagtctgagtcGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcg                                                     >  1:947642/1‑97 (MQ=255)
                                             tgagtctgagtcGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGtctgagtcg                                                     >  2:1439184/1‑97 (MQ=255)
                                             ggagtctgagtcGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcg                                                     <  1:327205/96‑1 (MQ=255)
                                              gagtctgagtcGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgc                                                    <  1:2474076/97‑1 (MQ=255)
                                                gtctgagtcGCTATCTGAATCGGTGTCTGAGTCTGAGACGCTATCGGAGTCTGAGTCGCTGTCTGAGGCTGAATCGCGTTCTGATtctgagtcgaag                                                  >  2:2920776/1‑94 (MQ=17)
                                                gtctgagtcGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctg                                                  <  1:1412143/97‑1 (MQ=255)
                                                gtctgagtcGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctg                                                  <  2:2831207/97‑1 (MQ=255)
                                                gtctgagtcGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATcccgagtcgccg                                                  >  1:1229347/1‑95 (MQ=255)
                                                  ctgagtcGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgaggcgcggtc                                                >  2:1157480/1‑97 (MQ=255)
                                                   tgagtcGCTATCTGAATCGCTGTCTGCGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGCATCGCTATCTGAGtctgagtcgctgtct                                               >  2:1316965/1‑97 (MQ=255)
                                                   tgagtcGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgaggcgctgtcg                                               >  2:2958379/1‑96 (MQ=255)
                                                    caatcGCAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctg                                              <  2:523995/94‑1 (MQ=255)
                                                     agtcGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTGTCTGATtctgagtcgctgtctga                                             >  2:933432/1‑97 (MQ=255)
                                                      gtcGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgag                                            <  1:239246/97‑1 (MQ=255)
                                                       tcGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagt                                           <  2:2761160/97‑1 (MQ=255)
                                                         gCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtct                                         >  1:1358066/1‑97 (MQ=255)
                                                         gCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtct                                         <  1:1916063/97‑1 (MQ=255)
                                                         gCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtct                                         <  2:2519403/97‑1 (MQ=255)
                                                            aTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgag                                      <  1:645120/97‑1 (MQ=255)
                                                                  aTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGtctgagtcgctgtctgaggctgagtcgctg                                >  2:190996/1‑97 (MQ=255)
                                                                     gCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgagtcgctgtct                             >  1:2602035/1‑97 (MQ=255)
                                                                        gtctgggtctgaGTCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgagtcgctgtctgag                          <  2:1664751/97‑1 (MQ=255)
                                                                        gtctgagtctgaGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgagtcgctgtctgag                          <  2:2475988/97‑1 (MQ=255)
                                                                            gagtctgagtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGagtcgctgtctgagtctgagtcgctgtctgagtctg                      <  1:2129072/97‑1 (MQ=255)
                                                                              gtctgagtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGaa                    <  1:2174602/97‑1 (MQ=255)
                                                                               tctgagtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAt                   <  1:2978212/97‑1 (MQ=255)
                                                                               tctgagtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAgtcgctgtctgagtctgagtcgctgtctgagtctgact                   >  2:2438858/1‑95 (MQ=255)
                                                                                ctgagtcGCTATCTGAATCTGTATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATc                  <  2:2854368/97‑1 (MQ=255)
                                                                                ctgagtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATc                  >  1:2061552/1‑97 (MQ=255)
                                                                                   agtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCt               <  1:737613/97‑1 (MQ=255)
                                                                                    gtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGtctgagtctgagtcgctgtctgagtctgagtcgctg              >  2:1366236/1‑97 (MQ=255)
                                                                                    gtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTg              <  1:994366/97‑1 (MQ=255)
                                                                                    gtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCGGTCTGAGTCCGAATCGCTg              >  2:2204214/1‑97 (MQ=255)
                                                                                    gtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCta              >  2:1887871/1‑96 (MQ=14)
                                                                                       gCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtct           >  1:117586/1‑97 (MQ=255)
                                                                                         ttgctgaatctgagtCGCTGTCTGAGTCTGATTCGCTATCTGATTCTGAGTCTCTGTCTGAGTCTTAGTCGCTGTCTGAGTCTGAATCGCTGtctga         <  2:377148/94‑1 (MQ=14)
                                                                                         tATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAgtctgagtcgctgtctgagtctgagtcgctgtctga         >  2:787540/1‑97 (MQ=255)
                                                                                         tATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctga         >  1:3022153/1‑97 (MQ=255)
                                                                                         tATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctga         <  1:1609472/97‑1 (MQ=255)
                                                                                          atctgaatctgaATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATc                  >  1:1252035/1‑87 (MQ=255)
                                                                                          atctgaatctgaATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctgaa        <  1:2749434/97‑1 (MQ=255)
                                                                                           tctgaatctgaaTCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctgaat       <  1:2952806/97‑1 (MQ=255)
                                                                                           tctgaatctgaaTCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctgaat       <  2:2398042/97‑1 (MQ=255)
                                                                                             tgaatctgaatcGCTGTCTGAGTCTGAATCGCTATCCGAGTCTGAGTCGCTGCCTGAGTCTGCCTCGCTGTCTGAGCCTGACTCGCCGtctgagtct     >  2:2153975/1‑97 (MQ=11)
                                                                                               aatctgaatcGCTGTCTGAGTCTGCATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctgaatctga   <  1:2385049/97‑1 (MQ=255)
                                                                                               aatctgaatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctgaatctga   <  1:1044572/97‑1 (MQ=255)
                                                                                                atctgaatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctgaatctgaa  <  1:2267304/97‑1 (MQ=255)
                                                                                                atctgaatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctgaatctgaa  <  2:735545/97‑1 (MQ=255)
                                                                                                |                                                                                                
ATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAA  >  BX571857/2700035‑2700227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: