Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 2,700,245 A→G 5.1% D741D (GAT→GAC SAS2516 ← fibrinogen and keratin‑10 binding surface anchored protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX5718572,700,2450AG 5.1% 871.5 / 8.2 249D741D (GAT→GACSAS2516fibrinogen and keratin‑10 binding surface anchored protein
Reads supporting (aligned to +/- strand):  ref base A (110/118);  new base G (12/4);  total (124/125)
Fisher's exact test for biased strand distribution p-value = 6.73e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 253 total aligned reads displayed.

TCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTG  >  BX571857/2700150‑2700341
                                                                                               |                                                                                                
tctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAAt                                                                                                 >  2:2499386/1‑97 (MQ=255)
  tgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCg                                                                                               >  2:1973233/1‑97 (MQ=255)
   gaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGc                                                                                              <  1:696177/97‑1 (MQ=255)
    agtCGCTCTCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCt                                                                                             <  2:2049104/95‑1 (MQ=255)
    aaTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCt                                                                                             <  2:625899/97‑1 (MQ=255)
     aTCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTa                                                                                            <  1:2078314/97‑1 (MQ=255)
     aTCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTa                                                                                            <  1:337306/97‑1 (MQ=255)
     aTCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTa                                                                                            >  1:1900234/1‑97 (MQ=255)
       cGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtc                                                                                          >  1:2341183/1‑97 (MQ=255)
       cGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtc                                                                                          >  1:1281761/1‑97 (MQ=255)
       cGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtc                                                                                          >  1:1794650/1‑97 (MQ=255)
        gCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTAtct                                                                                         >  2:2438848/1‑97 (MQ=255)
        gCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtct                                                                                         <  1:1954412/97‑1 (MQ=255)
         cTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTAtccg                                                                                        <  2:2316033/97‑3 (MQ=1)
          tgtCTGAGTCGGAGTCGCTGTCTGAATCGGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAATCGCTAtctga                                                                                       <  2:482220/95‑1 (MQ=11)
          tATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctga                                                                                       >  1:891830/1‑97 (MQ=255)
          tATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctga                                                                                       >  2:1144803/1‑97 (MQ=255)
          tATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctga                                                                                       <  2:838209/97‑1 (MQ=255)
            tCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctgagt                                                                                     <  2:1547462/97‑1 (MQ=255)
            tCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAATCCGAAGCGCTTCCTGAGGCGGAATCGCGAtctgcat                                                                                     >  2:123650/1‑94 (MQ=14)
              tGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGGGTCGGAAGCGGTGTCTGGGTCGGGAGCGGTAtctgagggt                                                                                   >  2:1121471/1‑94 (MQ=14)
              tGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctgagtct                                                                                   >  1:644942/1‑97 (MQ=255)
              tGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctgagtct                                                                                   <  1:2549172/97‑1 (MQ=255)
               gATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTATCTGAATCTGACTCACTGtctgattctg                                                                                  <  2:1265755/97‑1 (MQ=1)
                aTTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAATCGCTAtctgagtctga                                                                                 >  2:1751232/1‑97 (MQ=255)
                  tctgagtcgctgtctgagtctgagtcgctgtctgagTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATctgagtctgagt                                                                               <  2:809317/97‑1 (MQ=255)
                   ctgagtcgctgtctgagtctgagtcgctgtctgagtCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGCGTCTGACTCGCTATCtgagtctgagtc                                                                              >  2:2698525/1‑97 (MQ=255)
                   ctgagtcgctgtctgagtctgagtcgctgtctgagtCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCtgagtctgagtc                                                                              >  1:1705793/1‑97 (MQ=255)
                       gtcgctgtctgagtccgagtcgctgtctgagtctgaGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTg                                                                          <  2:2852872/97‑1 (MQ=255)
                        tcgctgtctgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGt                                                                         >  2:2757053/1‑97 (MQ=255)
                         cgctgtctgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTc                                                                        >  1:2272705/1‑97 (MQ=255)
                           ctgtctgagtcggagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTg                                                                      <  2:2945892/97‑1 (MQ=255)
                            tgtctgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGa                                                                     >  1:1857768/1‑97 (MQ=255)
                            tgtctgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGa                                                                     <  1:1864565/97‑1 (MQ=255)
                            tgtctgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGa                                                                     <  1:1327580/97‑1 (MQ=255)
                             gtctgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGaa                                                                    <  2:1915643/97‑1 (MQ=255)
                              tctgagtctgagtcgctgtctgcgtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAAt                                                                   <  2:314066/97‑1 (MQ=255)
                              tctgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAAt                                                                   <  1:108905/97‑1 (MQ=255)
                                tgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAATCt                                                                 >  2:2059034/1‑97 (MQ=255)
                                tgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCt                                                                 <  1:2054197/97‑1 (MQ=255)
                                tgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCt                                                                 >  1:1252735/1‑97 (MQ=255)
                                 gagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTg                                                                <  1:2987975/97‑1 (MQ=255)
                                  agtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCCGAGTCGCTGTCTGAAGCTGa                                                               >  2:419831/1‑97 (MQ=11)
                                     ctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTc                                                            <  1:1989232/97‑1 (MQ=255)
                                      tgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCa                                                           >  1:1618789/1‑97 (MQ=255)
                                         gtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCCCTa                                                        >  1:1114688/1‑97 (MQ=255)
                                         gtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTa                                                        <  1:1445727/97‑1 (MQ=255)
                                         gtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTa                                                        <  1:2403756/97‑1 (MQ=255)
                                         gtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCCGAGTCACTGTCTGAATCTGACTCACTa                                                        >  2:907789/1‑97 (MQ=255)
                                          tcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTat                                                       <  2:1817540/97‑1 (MQ=255)
                                           cgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCCCTGTCTGACTCTGAGTCACTatc                                                      >  2:129003/1‑97 (MQ=14)
                                             ctgtctgagtctgaATCGCTGTCTGACTCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctg                                                    <  2:1813604/97‑1 (MQ=255)
                                             ctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctg                                                    <  1:706203/97‑1 (MQ=255)
                                             ctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctg                                                    <  2:2350439/97‑1 (MQ=255)
                                               gtctgagtctgaATCGCTGTCTGAAGCGGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctgaa                                                  <  1:1359383/97‑1 (MQ=255)
                                                 ctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctgaatc                                                <  2:2295403/97‑1 (MQ=255)
                                                         gaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGc                                        <  1:973190/97‑1 (MQ=255)
                                                          aaTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctga                                                   >  2:926147/1‑85 (MQ=255)
                                                            tCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTAt                                     <  1:738687/97‑1 (MQ=39)
                                                            tCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTAt                                     <  2:2834463/97‑1 (MQ=37)
                                                             cGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTAtc                                    >  1:1540375/1‑97 (MQ=39)
                                                             cGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTAtc                                    >  1:1811916/1‑97 (MQ=39)
                                                                tatctgaatctgaaTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAc                                                              <  2:1245676/66‑1 (MQ=255)
                                                                tGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTAtctga                                 >  1:3009690/1‑97 (MQ=38)
                                                                tGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTAtctga                                 <  1:2680507/97‑1 (MQ=38)
                                                                 gTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTAtctgag                                >  1:1663349/1‑97 (MQ=40)
                                                                  tctgaatctgaaTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTAtctgagt                               >  1:2601451/1‑97 (MQ=255)
                                                                   ctgaatcggaatCGCTGTCGGAGTCTGAATCGGTGTCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTAtctgagtc                              <  2:2120620/97‑1 (MQ=14)
                                                                    tgaatctgaatcGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTAtctgagtct                             >  1:636612/1‑97 (MQ=255)
                                                                      aatctgaatcGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctgaatctga                                             >  2:1396904/1‑79 (MQ=38)
                                                                      aatctgaatcGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTAtctgagtctga                           >  2:1882067/1‑97 (MQ=40)
                                                                         ctgaatcGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCtgagtctgagtc                        <  2:1165514/97‑1 (MQ=255)
                                                                          tgaatcGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTc                                                            >  1:1503168/1‑60 (MQ=38)
                                                                          tgaatcGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcg                       >  1:1840192/1‑97 (MQ=255)
                                                                          tgaatcGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcg                       <  1:810455/97‑1 (MQ=255)
                                                                          tgaatcGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcg                       >  2:691474/1‑97 (MQ=40)
                                                                          tgaatcGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcg                       >  1:2046558/1‑97 (MQ=255)
                                                                           gaatcGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcgc                      <  1:1913388/97‑1 (MQ=255)
                                                                             atcTCTGTCTGGGTCCTAATCGCTCTCTGAGTCTAAGTCACTCTCTGAATCTGACTCTCTAACCGCATATGAGTCGCTATCTGAGTCTGAGtcgctg                    >  2:193334/1‑97 (MQ=14)
                                                                                gCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcgctgtct                 >  2:145087/1‑97 (MQ=255)
                                                                                gCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcgctggct                 >  1:2732143/1‑97 (MQ=255)
                                                                                gCTGCCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACCCACTATCTGAATCTGAGTCGCTATCTGAGGCTGAGtcgctgtct                 >  1:336302/1‑97 (MQ=255)
                                                                                  tGTCTGCGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTAACTGAATCTGAGCCGCTATCTGCGTCTGCGtcgctgtccga               >  2:1961314/1‑97 (MQ=14)
                                                                                   gtctgagtctgaATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcgctgtctgag              >  1:278840/1‑97 (MQ=255)
                                                                                   gtctgagtctgaATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcgctgtctgag              <  1:2112273/97‑1 (MQ=255)
                                                                                    tctgagtctgaCTCGCTATCTGAGTCGGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcgctgtctgagt             <  2:3007233/97‑1 (MQ=255)
                                                                                      tgagtctgaATCGCTAGCTGAGTCTGAGTCGCTGACTGAATCTGACTCACTGTGTGCATCGGAGTCGCTATCTGCGTCGGAAtcgctgtctgagtct           >  2:1783727/1‑97 (MQ=11)
                                                                                       gagtctgaATCGCTATCTGTGTCTGAGTCGCTGTCTGAATCTGACTCACTATCTGATTCTGAGTCGCTATCTGAGTCTGAGtcgctgtctgagtctg          <  2:47216/97‑1 (MQ=255)
                                                                                       gagtctgaATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcgctgtctgagtctg          <  1:2887563/97‑1 (MQ=255)
                                                                                        tgtctgaATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcgctgtctgagtctga         <  1:1759284/96‑1 (MQ=255)
                                                                                        agtctgaATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcgctgtctgagtctga         >  1:371998/1‑97 (MQ=255)
                                                                                        agtctgaATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcgctgtctgagtctga         >  1:495019/1‑97 (MQ=255)
                                                                                        agtctgaATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcgctgtctgagtctga         <  2:2201692/97‑1 (MQ=255)
                                                                                           ctgaATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTa                                      >  2:2638012/1‑65 (MQ=37)
                                                                                           ctgaATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcgctgtctgagtctgaatc      >  1:1427113/1‑97 (MQ=255)
                                                                                           ctgaATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcgctgtctgagtctgaatc      >  2:199467/1‑97 (MQ=255)
                                                                                            tgaATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcgctgtctgagtctgaatcg     <  1:2310848/97‑1 (MQ=255)
                                                                                            tgaATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcgctgtctgagtctgaatcg     >  2:765675/1‑97 (MQ=255)
                                                                                            tgaATCGCTATCTGAGTCTGACTCACTGTCTGAATCTGACTCCCTATCTGAATCCGAATCGCTATCTGAGCCTGAGtcgctgtctgagtctgaatcg     >  2:1637478/1‑97 (MQ=11)
                                                                                               aTCGCTATCTGCGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcgctgtctgagtctgaatcgctg  <  1:2469588/97‑1 (MQ=255)
                                                                                               |                                                                                                
TCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTG  >  BX571857/2700150‑2700341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: