Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 431,505 T→C 7.8% S77P (TCA→CCA)  SAS0387 → exotoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX571857431,5050TC 7.8% 705.2 / 2.5 203S77P (TCA→CCA) SAS0387exotoxin
Reads supporting (aligned to +/- strand):  ref base T (91/87);  new base C (8/12);  total (102/101)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 208 total aligned reads displayed.

GCAAATGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGACACAT  >  BX571857/431409‑431601
                                                                                                |                                                                                                
gCAAATGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCACAAt                                                                                                  >  1:424650/1‑97 (MQ=255)
  aaaTGCAACAACACCATCTTCAACGAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACACCACCATCTTCAACTAAAGTAGAAGCACCGCAATCa                                                                                                <  2:2254707/97‑1 (MQ=255)
   aaTGCAACAACACCGTCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCaa                                                                                               <  2:604749/97‑1 (MQ=255)
    aTGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGACGCACCGCAAGCaaa                                                                                              >  2:2769312/1‑97 (MQ=255)
     tGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCaaaa                                                                                             >  2:478672/1‑97 (MQ=255)
     tGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGACTAACGTAGAAACACCGCACCCaaaa                                                                                             >  2:2079182/1‑97 (MQ=11)
      gccacaacaCCATCTTCAACTCAAGTGGAAGCACCGCAACAAGCAGCAAACGCGCCAACACCACCTTCAACTAAAGTAGCAACACCGCAACCACAAc                                                                                            >  2:194505/4‑97 (MQ=14)
       caacaaCACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAAcc                                                                                           >  1:222346/1‑97 (MQ=255)
       caacaaCACAATCTTCAACTAAAGTGGAAGCACCGGAACAAGCAGCAAACGCGACAACACCAACTTCAACTAAAGTAGAAGCACCCCAACAaaaagc                                                                                           >  2:1999448/1‑95 (MQ=17)
        aacaacACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCa                                                                                          <  1:871481/97‑1 (MQ=255)
        aacaacACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCa                                                                                          <  2:2137761/97‑1 (MQ=255)
        aacaacACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCa                                                                                          <  1:674420/97‑1 (MQ=255)
        aacaacACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCAACTTCAACTAAAGTAGAAGCACCGcaaccacaacca                                                                                          >  2:1042516/1‑97 (MQ=255)
           aacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAc                                                                                       >  1:1043695/1‑97 (MQ=255)
           aacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAACCAAAACCAAAc                                                                                       >  1:788923/1‑97 (MQ=255)
           aacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAACACCACAAACAAAACCAAAc                                                                                       >  2:1062902/1‑97 (MQ=255)
             ccccATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcgc                                                                                     <  1:2108570/95‑1 (MQ=255)
             cacCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcgc                                                                                     >  1:1616102/1‑97 (MQ=255)
              acCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcgcg                                                                                    <  2:1743346/97‑1 (MQ=255)
                cATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGac                                                                                  <  2:2603503/97‑1 (MQ=255)
                cATCTTAAACTAGAGTGGAAGCACCACAACAAGCAGCAACCGCCACAACACCATCTTCACCTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGac                                                                                  <  2:534174/97‑1 (MQ=21)
                 aTCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGACGCACCGCAATCAAAACCAAACGCGaca                                                                                 >  2:2465926/1‑97 (MQ=255)
                 aTCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGaca                                                                                 >  1:2336074/1‑97 (MQ=255)
                    ttCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCCTCTTCAACTAAAGTAGACGCACCGCAAACAAAACCACACGCCacaaca                                                                              >  2:2435324/1‑97 (MQ=21)
                      cAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAACCCAAACGCGACAACAcc                                                                            <  1:2932211/97‑1 (MQ=255)
                      cAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACAcc                                                                            >  1:558496/1‑97 (MQ=255)
                      cAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACAcc                                                                            <  2:2340995/97‑1 (MQ=255)
                      cAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACAcc                                                                            <  2:1099748/97‑1 (MQ=255)
                      cAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACAcc                                                                            <  1:137434/97‑1 (MQ=255)
                       aaCTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCa                                                                           <  1:2053303/97‑1 (MQ=255)
                       aaCTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCa                                                                           >  1:2774993/1‑97 (MQ=255)
                         cTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATc                                                                         <  1:1311200/97‑1 (MQ=255)
                         cTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATc                                                                         <  2:2364900/97‑1 (MQ=255)
                          tAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAACCGCGACAACACCATCt                                                                        >  2:2735745/1‑97 (MQ=255)
                          tAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCt                                                                        >  1:2426182/1‑97 (MQ=255)
                          tAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCt                                                                        >  1:2735248/1‑97 (MQ=255)
                            aaGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTc                                                                      >  1:2670407/1‑97 (MQ=255)
                             aGTGGAAGCGCCGGAGCAAGCAGGGAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCa                                                                     <  2:537404/97‑1 (MQ=21)
                             aGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCa                                                                     <  1:2552095/97‑1 (MQ=255)
                             aGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCa                                                                     <  1:227537/97‑1 (MQ=255)
                               tGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAAc                                                                   >  1:1426430/1‑97 (MQ=255)
                                ggAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACt                                                                  >  1:2648546/1‑97 (MQ=255)
                                ggAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACt                                                                  <  1:2515536/97‑1 (MQ=255)
                                ggAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACt                                                                  >  1:1391213/1‑97 (MQ=255)
                                 gAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAACGTAGAAGCACCGCAACCAAAACCAAACGCGACACCACCATCTTCAACTa                                                                 >  2:2969554/1‑97 (MQ=255)
                                    gCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAg                                                              <  1:520209/97‑1 (MQ=255)
                                     cACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAGAACCAAACGCGACAACACCATCTTCAACTAAAGt                                                             <  2:697887/97‑1 (MQ=255)
                                        cGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGa                                                          >  2:2295563/1‑97 (MQ=255)
                                         gCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGaa                                                         <  1:610527/97‑1 (MQ=255)
                                          caacaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGaac                                                        >  2:627848/1‑96 (MQ=255)
                                          caacaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAg                                                        >  1:631222/1‑97 (MQ=255)
                                           aacaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGc                                                       <  2:1188656/97‑1 (MQ=255)
                                           aacaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGc                                                       <  2:1998016/97‑1 (MQ=255)
                                                 cagcaAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGca                                                 >  1:2192856/1‑97 (MQ=255)
                                                   gcaAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaac                                               <  2:2816542/97‑1 (MQ=255)
                                                    caAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaaca                                              >  2:1506676/1‑97 (MQ=255)
                                                    caAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaaca                                              <  2:3026617/97‑1 (MQ=255)
                                                     aaaCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGCAGCACCGcaacaa                                             >  2:732377/1‑97 (MQ=255)
                                                       aCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAagc                                           <  1:1717516/97‑1 (MQ=255)
                                                         gcgACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAagcag                                         >  1:1898303/1‑97 (MQ=255)
                                                           gACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAgcagca                                       <  1:1032052/97‑1 (MQ=255)
                                                              aacaCCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAc                                    >  1:2799264/1‑97 (MQ=255)
                                                                cacCATCTTCAACTAAAGTAGAAGCACCGCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcgc                                  <  2:1823170/97‑1 (MQ=255)
                                                                 acCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcgcg                                 >  1:1209958/1‑97 (MQ=255)
                                                                 acCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcgcg                                 >  1:937150/1‑97 (MQ=255)
                                                                  ccATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGa                                >  1:1495309/1‑97 (MQ=255)
                                                                  ccATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGa                                >  1:731225/1‑97 (MQ=255)
                                                                   cATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGac                               <  1:1128262/97‑1 (MQ=255)
                                                                   cATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGac                               >  2:1213139/1‑97 (MQ=255)
                                                                     ccTTCAACTAAAGTAGAAGCACCGCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaa                             <  2:1549758/96‑1 (MQ=255)
                                                                        taaaCAAAAGAAGAAACACCGCAATCAAAACAAAACACGACAACAACAACTACAACTAAAGTGGAAACACCGCAACAAGCAGCAAACGAGacaacaa                          >  2:408472/3‑96 (MQ=18)
                                                                         cAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcacc                         <  1:174272/97‑1 (MQ=255)
                                                                             tAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCt                     >  1:2847579/1‑97 (MQ=255)
                                                                             tAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCt                     <  1:481615/97‑1 (MQ=255)
                                                                             tAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCt                     >  1:74911/1‑97 (MQ=255)
                                                                              aaaGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCtt                    >  2:1402400/1‑97 (MQ=255)
                                                                                aGTAGAAGCGCCGCAACCACGACCCAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCg                  <  2:1317775/97‑1 (MQ=17)
                                                                                aGTAGAAGCACCGCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCg                  <  2:900178/97‑1 (MQ=255)
                                                                                 gTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGt                 >  2:2169639/1‑97 (MQ=255)
                                                                                 gTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGt                 <  2:2527091/97‑1 (MQ=255)
                                                                                 gTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGt                 >  2:2638296/1‑97 (MQ=255)
                                                                                  tAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTc                >  2:758171/1‑97 (MQ=255)
                                                                                   aGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCt               >  1:1661096/1‑97 (MQ=255)
                                                                                   aGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCt               >  2:1238721/1‑97 (MQ=255)
                                                                                   aGAAGCACCGCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCt               <  2:1898644/97‑1 (MQ=255)
                                                                                     aaGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTaa             <  1:2503223/97‑1 (MQ=255)
                                                                                      aGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAAt            >  1:2187233/1‑97 (MQ=255)
                                                                                      aGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAAt            >  1:1987531/1‑97 (MQ=255)
                                                                                      aGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAAt            <  2:1097586/97‑1 (MQ=255)
                                                                                       gCACCGCAATCAAAACCAAACGCGACAACTCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCCAACGCGACAACACCACCTTCGTCTAATg           <  1:1005802/97‑1 (MQ=255)
                                                                                         aCCGCAATCAACACCAACCGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTa         <  1:366337/97‑1 (MQ=255)
                                                                                         aCCGCAATCAAAACCAACCGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCCCCTTCGTCTAATGTa         >  2:2406452/1‑97 (MQ=255)
                                                                                         aCCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTa         >  2:139265/1‑97 (MQ=255)
                                                                                         aCCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTa         <  1:2659491/97‑1 (MQ=255)
                                                                                           cGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGa       <  2:1879366/97‑1 (MQ=255)
                                                                                           cGCAATCAAAACCAAACGCGACAACACCAGCGTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACGCCACCTTCGTCTAATGTAGa       <  2:952649/97‑1 (MQ=255)
                                                                                            gCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGac      >  1:1274579/1‑97 (MQ=255)
                                                                                             cAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGaca     >  1:1089975/1‑97 (MQ=255)
                                                                                              aaTCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGacac    <  1:2205960/97‑1 (MQ=255)
                                                                                              aaTCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGacac    <  1:2604837/97‑1 (MQ=255)
                                                                                                |                                                                                                
GCAAATGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGACACAT  >  BX571857/431409‑431601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑