Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 843,239 A→G 5.5% N731D (AAC→GAC)  SAS0752 → clumping factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX571857843,2390AG 5.5% 938.9 / 3.7 252N731D (AAC→GAC) SAS0752clumping factor
Reads supporting (aligned to +/- strand):  ref base A (105/129);  new base G (11/7);  total (116/136)
Fisher's exact test for biased strand distribution p-value = 2.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

ACTCAGATAGCGATTCCGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATTCAGATTCCGACAGTGACTCAGATTCCGACAGTGACTCAGACTCAGACAGTG  >  BX571857/843144‑843335
                                                                                               |                                                                                                
actcagatagcgattccgactcagatagcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCaa                                                                                                 <  2:2061506/97‑1 (MQ=255)
 ctcagatagcgattccgactcagatagcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCGAc                                                                                                >  2:1501944/1‑97 (MQ=255)
 ctcagatagcgattccgactcagatagcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAAc                                                                                                >  1:2017328/1‑97 (MQ=255)
  tcagatagcgattccgactcagatagcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACa                                                                                               >  1:2926557/1‑97 (MQ=255)
     gatagcgattccgactcagatagcgaCTCAGATTCAGACAGCGATTCGGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTg                                                                                            <  1:2881915/97‑1 (MQ=255)
       tagcgattccgactcagatagcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTGAc                                                                                          >  1:2325568/1‑97 (MQ=255)
        ggcgatgccgactcagagggcgaCTCAGATTCAGACGGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACt                                                                                         <  2:1409824/96‑1 (MQ=18)
         gcgattccgactcagatagcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTc                                                                                        >  1:270882/1‑97 (MQ=255)
         gcgattccgactcagatagcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTc                                                                                        >  2:3004799/1‑97 (MQ=255)
          ggattccgactcagatagcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTCa                                                                                       <  2:484697/96‑1 (MQ=255)
            attccgactcagatagcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGa                                                                                     <  2:2102136/97‑1 (MQ=255)
              tccgactcagatagcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGAtt                                                                                   >  1:2369049/1‑97 (MQ=255)
              tccgactcagatagcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGAtt                                                                                   >  1:2713329/1‑97 (MQ=255)
               ccgactcagatagcgaCTCAGATTCCGACAGCGAGTCAGATTCAGACAGCGATGCAGTTTCAGATAGCGAGTCAGATTCCGCCAGGGACTCGGATTc                                                                                  >  2:2173751/1‑97 (MQ=17)
               ccgactcagatagcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGATTc                                                                                  >  1:51293/1‑97 (MQ=255)
                cgactcagatagcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGATTcc                                                                                 <  1:1312790/97‑1 (MQ=255)
                 gactcagatagcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGATTCCg                                                                                <  2:639223/97‑1 (MQ=255)
                  actcagatagcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGATTCCGa                                                                               <  2:1830021/97‑1 (MQ=255)
                    tcagatagcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCACGAGTGACTCAGATTCCGACa                                                                             >  2:2001845/1‑97 (MQ=255)
                    tcagatagcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACa                                                                             >  1:1557227/1‑97 (MQ=255)
                      agatagcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGt                                                                           >  1:265404/1‑97 (MQ=255)
                       gatagcgaCTCAGATTCAGACAGCGATTCAGATTCAGACATCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTg                                                                          <  2:1026161/97‑1 (MQ=255)
                       gatagcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTg                                                                          <  1:2554350/97‑1 (MQ=255)
                          agcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACt                                                                       <  2:978017/97‑1 (MQ=255)
                           gcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCGACAGTGACTCAGATTCAGACAGTGACTc                                                                      >  1:973358/1‑97 (MQ=255)
                           gcgaCTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTc                                                                      >  1:359093/1‑97 (MQ=255)
                            cggcTCAGCTTCAGACAGCGATTCAGATTCGGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGGGACGCAGATTCCGACAGTGACTCg                                                                     <  2:1134057/94‑1 (MQ=18)
                               cTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGAt                                                                  >  2:1236111/1‑97 (MQ=255)
                                tcagattcagacagcgattcagattcagacagcgatTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGAtt                                                                 <  1:172189/97‑1 (MQ=255)
                                tcagattcagacagcgattcagattcagacagcgatTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGAtt                                                                 <  1:728602/97‑1 (MQ=255)
                                tcagattcagacagcgattcagattcagacagcgatTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGAtt                                                                 <  1:680841/97‑1 (MQ=255)
                                tcagattcagacagcgattcagattcagacagcgatTCAGATTCAGATAGCGATTCAGATTCAGACAGTGACTCAGATTCCGACAGTGACTCGGact                                                                 >  2:1870241/1‑95 (MQ=255)
                                    attcagacagcgattcagattcagacagcgattcagATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGa                                                             <  1:1012624/97‑1 (MQ=255)
                                    attcagacagcgattcagattcagacagcgattcagATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGa                                                             <  2:1104649/97‑1 (MQ=255)
                                    attcagacagcgattcagattcagacagcgattcagATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGa                                                             <  2:224364/97‑1 (MQ=255)
                                       cagacagcgattcagattcagacagcgattcagattCAGATAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAg                                                          >  1:1997426/1‑97 (MQ=255)
                                        agacagcgattcagattcaggcagcgattcagattcAGATAGCGATTCAGATGCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGc                                                         <  2:1773601/97‑1 (MQ=255)
                                        agacagcgattcagattcagacagcgattcagattcAGATAGCGATTCAGATTCCAACAGTGACTCAGATGCCGACAGTGACTCGGATTCAGATAGc                                                         <  1:1327517/97‑1 (MQ=255)
                                         gacagcgatgcagattcagacagctattcagattcaGATAGCGAGTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCg                                                        <  2:360359/97‑1 (MQ=255)
                                           cagcgattcagattcagacagcgattcagattcagaTAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGAt                                                      >  1:38182/1‑97 (MQ=255)
                                           cagcgattcagattcagacagcgattcagattcagaTAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGAt                                                      >  1:2296036/1‑97 (MQ=255)
                                            agcgattcagattcagacagcgattcagattcagaTAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGAtt                                                     <  2:523743/97‑1 (MQ=255)
                                            agcgattcagattcagacagcgattcagattcagaTAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGAtt                                                     >  1:2522583/1‑97 (MQ=255)
                                            agcgattcagattcagacagcgattcagattcagaTAGCGACTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGAtt                                                     >  2:265682/1‑97 (MQ=255)
                                              cgattcagattcagacagcgattcagattcagaTAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtca                                                   <  2:164718/97‑1 (MQ=255)
                                               gattcagattcagacagcgattcagattcagaTAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcag                                                  >  2:1450806/1‑97 (MQ=255)
                                               gagtcagattcagacagcgattcagattcagaTAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcag                                                  <  2:2511736/94‑1 (MQ=255)
                                                attcagagtcagacagcgactcagattcagaTAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcaga                                                 <  1:136829/97‑1 (MQ=255)
                                                 ttcagattcagacagcgattcagattcagaTAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagat                                                >  1:2821302/1‑97 (MQ=255)
                                                 ttcagattcagacagcgattcagattcagaTAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagat                                                >  2:2321806/1‑97 (MQ=255)
                                                 ttcagattcagacagcgattcagattcagaTAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagat                                                <  1:1959310/97‑1 (MQ=255)
                                                  tcagattcagacagcgattcagattcagaTAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACCGTGCCTCGGATTCAGATAGCGATtcagatt                                               >  2:1560874/1‑97 (MQ=255)
                                                  tcagattcagacagcgattcagattcagaTAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagatt                                               <  1:1217189/97‑1 (MQ=255)
                                                   cagattcagacagcgattcagattcagaTAGCGATTCAGATTCCAACAGTGACGCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattc                                              >  2:1661341/1‑97 (MQ=255)
                                                        tcagtcggcgattcagattcagaCAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgaca                                         <  2:161946/97‑1 (MQ=255)
                                                         cagacagcgattcagattcagaTAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacag                                        >  2:427287/1‑97 (MQ=255)
                                                           gacagcgattcagattcagaTAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtg                                      <  1:831645/97‑1 (MQ=255)
                                                            tccgcgcttcagattcagaTCGCGATTCCGATTCCGACAGCGATTCCGCTTCCGGCAGTGACTCGGATTCAGATAGCGATtcagattccgacagtga                                     <  2:1301828/94‑1 (MQ=17)
                                                                cgattcagattcagaTAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtgactca                                 <  2:1839723/97‑1 (MQ=255)
                                                                cgattcagattcagaTAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattcagacagtgactca                                 >  2:2365803/1‑97 (MQ=255)
                                                                 gattcagattcagaTAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtgactcag                                >  1:2599122/1‑97 (MQ=255)
                                                                 gattcagattcagaTAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtgactcag                                >  1:2089326/1‑97 (MQ=255)
                                                                  attcagattcagaTAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtgactcaga                               >  2:831284/1‑97 (MQ=255)
                                                                  attcagagtcagaGAGCGATTCAGATTCCAACCTGGACTCAGGGGCCGACAGTGACGCGGATTCAGATAGCGATtcagattccgacagtggctcaga                               <  2:930697/97‑1 (MQ=21)
                                                                   ttcagattcagaTAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGACtcagattcagacagcgactccgat                              >  2:2950140/1‑97 (MQ=18)
                                                                    tcagattcagatAGCGATTCAGATTCCAACAGGGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtgactcagatt                             <  2:1967065/97‑1 (MQ=255)
                                                                     cagattcagatAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtgactcagattc                            >  1:2384704/1‑97 (MQ=255)
                                                                     cagattcagatAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagggacccagattc                            >  2:555292/1‑97 (MQ=255)
                                                                      agtttcagatAGCGCTTCAGATTCCGACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtgactcagattcc                           <  2:2374305/94‑1 (MQ=255)
                                                                      agattcagatAGCGATTCAGATTCTAACAGTGGCTCAGATTCGGACAGTGAATCGGATTCAGGTGGCGATtcaggttccgacagtgactcagattcc                           <  1:835840/97‑1 (MQ=18)
                                                                      agattcagatAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtgactcagattcc                           <  1:1756795/97‑1 (MQ=255)
                                                                        attcagatAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtgactcagattccga                         <  1:1112802/97‑1 (MQ=255)
                                                                         ttcagatAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATCGCGATtcagattccgacagtgactcagattccgac                        >  2:1700608/1‑97 (MQ=255)
                                                                         ttcagatAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtgactcagattccgac                        <  1:1915537/97‑1 (MQ=255)
                                                                         ttcagatAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtgactcagattccgac                        <  2:529042/97‑1 (MQ=255)
                                                                         ttcagatAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtgactcagattccgac                        <  1:2266177/97‑1 (MQ=255)
                                                                          tcagatAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtgactcagattccgaca                       <  1:1639722/97‑1 (MQ=255)
                                                                          tcagatAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtgactcagattccgaca                       >  2:86445/1‑97 (MQ=255)
                                                                            agatAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtgactcagattccgacagt                     >  1:144406/1‑97 (MQ=255)
                                                                             gatAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtgactcagattccgacagtg                    <  1:1814917/97‑1 (MQ=255)
                                                                             gatAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtgactcagattccgacagtg                    <  1:867140/97‑1 (MQ=255)
                                                                             gatAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtgactcagattccgacagtg                    >  2:2434378/1‑97 (MQ=255)
                                                                              atAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtgactcagattccgacagtga                   <  2:105915/97‑1 (MQ=255)
                                                                               tAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtgactcagattccgacagtgac                  >  1:1386021/1‑97 (MQ=255)
                                                                               tAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATtcagattccgacagtgactcagattccgacagtgac                  >  2:2801504/1‑97 (MQ=255)
                                                                                 gCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATTCagattccgacagtgactcagattccgacagtgactc                >  1:1697160/1‑97 (MQ=255)
                                                                                 gCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATTCagattccgacagtgactcagattccgacagtgactc                >  1:1267301/1‑97 (MQ=255)
                                                                                 gCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATTCagattccgacagtgactcagattccgacagtgactc                >  2:1683935/1‑97 (MQ=255)
                                                                                     ttCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATTCAGATTCCGACAGTGACTCAGATTCCGACAGTgactcagac            >  1:781616/1‑97 (MQ=255)
                                                                                      tCATATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATTCAGATTCCGACAGTGACTCAGATTCCGACAGTgactcagact           <  2:673897/97‑1 (MQ=255)
                                                                                      tCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATTCAGATTCCGACAGTGACTCAGATTCCGACAGTgactcagact           >  2:1728206/1‑97 (MQ=255)
                                                                                      tCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATTCAGATTCCGACAGTGACTCAGATTCCGACAGCgactcagact           >  1:2991827/1‑97 (MQ=255)
                                                                                       cAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATTCAGATTCCGACAGTGACTCAGATTCCGACAGTgactcagactc          >  1:2182442/1‑97 (MQ=255)
                                                                                       cAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATTCAGATTCCGACAGTGACTCAGATTCCGACAGTgactcagactc          >  1:576686/1‑97 (MQ=255)
                                                                                         gATTCCAACAGTGCCTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATTCAGATTCCGACAGTGACTCAGATTCCGACAGTGactcagacgcag        <  2:2599028/97‑1 (MQ=255)
                                                                                          aTTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATTCAGATTCCGACAGTGACTCAGATTCCGACAGTGActcagactcaga       <  1:217809/97‑1 (MQ=255)
                                                                                           ttCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGCTAGCGATTCAGACTCCGCCAGTGACTCCGATTCCGCCAGTGActccgactcagaa      >  2:762173/1‑96 (MQ=18)
                                                                                           ttCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATTCAGATTCCGACAGTGACTCAGATTCCGACAGTGACtcagactcagac      <  1:553293/97‑1 (MQ=255)
                                                                                           ttCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATTCAGATTCCGACAGTGACTAAGAATCCGACAGTGACtcagactcagac      >  2:2901398/1‑97 (MQ=255)
                                                                                            tCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATTCAGATTCCGACAGTGACTCAGATTCCGACAGTGACTCAGACTCAGACa     >  1:2470412/1‑97 (MQ=255)
                                                                                               |                                                                                                
ACTCAGATAGCGATTCCGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCCAACAGTGACTCAGATTCCGACAGTGACTCGGATTCAGATAGCGATTCAGATTCCGACAGTGACTCAGATTCCGACAGTGACTCAGACTCAGACAGTG  >  BX571857/843144‑843335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑