Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 431,505 T→C 8.2% S77P (TCA→CCA)  SAS0387 → exotoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX571857431,5050TC 8.2% 759.4 / 8.7 215S77P (TCA→CCA) SAS0387exotoxin
Reads supporting (aligned to +/- strand):  ref base T (100/91);  new base C (13/8);  total (115/100)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

AATGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGACACAT  >  BX571857/431412‑431601
                                                                                             |                                                                                                
aaTGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCaa                                                                                               <  2:710091/97‑1 (MQ=255)
aaTGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCAACTAAAGTAGAAGCACCGCAATCaa                                                                                               >  2:1258640/1‑97 (MQ=255)
 aTGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGTAGCAAACGCGACAACACCACCTTCAACTAAAGTAGACACACCACAACCaca                                                                                              >  2:2823017/1‑95 (MQ=11)
 aTGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCaaa                                                                                              <  1:1723612/97‑1 (MQ=255)
  tGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAAACaaaa                                                                                             >  1:1295346/1‑97 (MQ=255)
   gCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAACCAGCAAACGCGACAACACCATCTTCACCTAAAGTAGAAGCACCGCAATCaaaaa                                                                                            >  1:1563686/1‑96 (MQ=255)
    caacaaCACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAAcc                                                                                           >  1:2650282/1‑97 (MQ=255)
     aacaacACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCa                                                                                          <  1:931526/97‑1 (MQ=255)
     aacaacACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCa                                                                                          <  1:2160972/97‑1 (MQ=255)
     aacaacACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCa                                                                                          <  2:2953658/97‑1 (MQ=255)
      acaacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCaa                                                                                         <  2:3496091/97‑1 (MQ=255)
       caacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGACTAAAGTAGAAGCACCACAACCAAAACCaca                                                                                        >  1:1971996/1‑95 (MQ=14)
        aacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAc                                                                                       >  1:775672/1‑97 (MQ=255)
        aacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAc                                                                                       >  1:2500281/1‑97 (MQ=255)
          cacCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGACGCACCGCAACCAAAACCAAAcgc                                                                                     >  2:3079189/1‑97 (MQ=255)
          cacCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcgc                                                                                     <  2:3183650/97‑1 (MQ=255)
           acCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcgcg                                                                                    <  1:817168/97‑1 (MQ=255)
           acCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAACCAAAACCAACcgcg                                                                                    >  2:269745/1‑97 (MQ=255)
           acCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCAACTAAAGTAGACACACCACAATCAAAACCAACcgcc                                                                                    >  2:1803417/1‑96 (MQ=14)
              aTCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGaca                                                                                 >  1:951557/1‑97 (MQ=255)
               tCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGacaa                                                                                <  2:898541/97‑1 (MQ=255)
               tCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGacaa                                                                                <  1:2745053/97‑1 (MQ=255)
               tCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGacaa                                                                                >  2:3046053/1‑97 (MQ=255)
                cTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGacaac                                                                               >  1:1697995/1‑97 (MQ=255)
                cTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGacaac                                                                               <  1:2891621/97‑1 (MQ=255)
                cTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCACAAACAAAACCAACAGCGacaac                                                                               >  2:700556/1‑97 (MQ=21)
                 ttCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGacaaca                                                                              >  1:1026652/1‑97 (MQ=255)
                  tCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAacac                                                                             <  1:2374103/97‑1 (MQ=255)
                  tCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAacac                                                                             <  2:507050/97‑1 (MQ=255)
                  tCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAacac                                                                             <  2:777538/97‑1 (MQ=255)
                   cAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACAcc                                                                            <  1:3376881/97‑1 (MQ=255)
                    aaCTAAAGTGGAAGCACCGCAACAAGCCGCAAACGCGACAACACCATCTACAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCa                                                                           <  2:1958460/97‑1 (MQ=255)
                    aaCTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCa                                                                           <  1:554820/97‑1 (MQ=255)
                     aCTAAAGTGGAAGCACCGCAACCAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAACCAGCACCAAACGCGACAACACCac                                                                          <  2:1170360/97‑2 (MQ=14)
                     aCTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCAt                                                                          <  2:849589/97‑1 (MQ=255)
                     aCTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCAt                                                                          <  1:3612709/97‑1 (MQ=255)
                        aaaGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCACACGCGACAACACCAGCtc                                                                       >  2:1478047/1‑96 (MQ=255)
                        aaaGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCtt                                                                       <  2:1699530/97‑1 (MQ=255)
                         aaGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTc                                                                      <  2:570063/97‑1 (MQ=255)
                          aGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCa                                                                     <  2:2083517/97‑1 (MQ=255)
                          aGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCa                                                                     <  1:2178793/97‑1 (MQ=255)
                          aGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCa                                                                     <  2:489861/97‑1 (MQ=255)
                           gTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCaa                                                                    >  1:431646/1‑97 (MQ=255)
                             ggAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACt                                                                  >  2:324219/1‑97 (MQ=255)
                              gAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTa                                                                 <  2:2897523/97‑1 (MQ=255)
                               aaGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTaa                                                                <  1:2574250/97‑1 (MQ=255)
                                aGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTaaa                                                               <  2:2609359/97‑1 (MQ=255)
                                aGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTaaa                                                               >  1:471448/1‑97 (MQ=255)
                                aGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTaaa                                                               <  2:1241409/97‑1 (MQ=255)
                                  cACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGt                                                             >  1:2378756/1‑97 (MQ=255)
                                  cACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGt                                                             >  2:2503083/1‑97 (MQ=255)
                                   aCCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCCACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTg                                                            >  2:423115/1‑97 (MQ=255)
                                        aacaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGc                                                       <  2:1428492/97‑1 (MQ=255)
                                        aacaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACAAAAGTGGAAGc                                                       >  2:1191282/1‑97 (MQ=255)
                                           aaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCAcc                                                    >  1:332796/1‑97 (MQ=255)
                                            agcagcAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCg                                                   >  2:2182481/1‑97 (MQ=255)
                                              cagcaAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAATACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGca                                                 <  1:1839259/97‑1 (MQ=255)
                                              cagcaAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGca                                                 >  2:2161095/1‑97 (MQ=255)
                                               agcaAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaa                                                >  2:3157750/1‑97 (MQ=255)
                                                gcaAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaac                                               >  1:3193757/1‑97 (MQ=255)
                                                 caAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaaca                                              >  1:2150060/1‑97 (MQ=255)
                                                  aaaCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaacaa                                             <  2:143592/97‑1 (MQ=255)
                                                  aaaCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaacaa                                             >  1:1788285/1‑97 (MQ=255)
                                                  aaaCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaacaa                                             >  1:1440262/1‑97 (MQ=255)
                                                  aaaCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaacaa                                             >  2:2433140/1‑97 (MQ=255)
                                                    aCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAagc                                           >  1:3149981/1‑97 (MQ=255)
                                                     cgcgACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAagca                                          >  2:2643130/1‑97 (MQ=255)
                                                      gcgACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAagcag                                         >  1:2133426/1‑97 (MQ=255)
                                                      gcgACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAagcag                                         >  1:114438/1‑97 (MQ=255)
                                                        gACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAgcagca                                       <  1:2481496/97‑1 (MQ=255)
                                                        gACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAgcagca                                       <  1:3566087/97‑1 (MQ=255)
                                                           aacaCCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAc                                    >  2:1987805/1‑97 (MQ=255)
                                                           aacaCCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAc                                    >  1:2248637/1‑97 (MQ=255)
                                                             cacCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcgc                                  <  1:1242048/97‑1 (MQ=255)
                                                              acCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcgcg                                 >  1:1233909/1‑97 (MQ=255)
                                                               ccATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAACCGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGa                                >  2:2703524/1‑97 (MQ=255)
                                                                cATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGac                               <  1:1979806/97‑1 (MQ=255)
                                                                    ttCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaaca                           >  1:1541388/1‑97 (MQ=255)
                                                                    ttCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaaca                           >  1:2565293/1‑97 (MQ=255)
                                                                      cAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcacc                         >  2:2222271/1‑97 (MQ=255)
                                                                      cAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcacc                         <  2:3338463/97‑1 (MQ=255)
                                                                      cAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCAGCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcacc                         >  2:1019141/1‑97 (MQ=255)
                                                                       aaCTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcacca                        >  2:2094730/1‑97 (MQ=255)
                                                                        aCTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcaccac                       >  2:1391023/1‑97 (MQ=255)
                                                                         cTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACaccacc                      <  2:1282301/97‑1 (MQ=255)
                                                                         cTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACaccacc                      >  2:3107870/1‑97 (MQ=255)
                                                                          tAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACaccaccc                     >  2:3037913/1‑96 (MQ=255)
                                                                          tAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCt                     >  1:3468688/1‑97 (MQ=255)
                                                                          tAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCt                     <  2:1348095/97‑1 (MQ=255)
                                                                          tAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCt                     >  2:2323828/1‑97 (MQ=255)
                                                                            aaGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTc                   <  1:6848/97‑1 (MQ=255)
                                                                             aGTAGAAGCACCGCAATCACAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCg                  <  2:1917595/97‑1 (MQ=255)
                                                                             aGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCg                  <  1:2045990/97‑1 (MQ=255)
                                                                             aGTAGAAGCACCGCAACCAACACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCg                  <  2:380219/97‑1 (MQ=255)
                                                                                 gAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTa              >  1:2629355/1‑97 (MQ=255)
                                                                                    gCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATg           >  1:3264095/1‑97 (MQ=255)
                                                                                     cACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGt          >  2:321872/1‑97 (MQ=255)
                                                                                      aCCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTa         <  1:3639156/97‑1 (MQ=255)
                                                                                          cAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGaca     >  1:615174/1‑97 (MQ=255)
                                                                                            aTCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGAcaca   <  1:3491685/97‑1 (MQ=255)
                                                                                             |                                                                                                
AATGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGACACAT  >  BX571857/431412‑431601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑