Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 431,505 T→C 9.2% S77P (TCA→CCA)  SAS0387 → exotoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX571857431,5050TC 9.2% 934.8 / 13.0 257S77P (TCA→CCA) SAS0387exotoxin
Reads supporting (aligned to +/- strand):  ref base T (111/115);  new base C (19/10);  total (131/126)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

GCAAATGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGACACAT  >  BX571857/431409‑431601
                                                                                                |                                                                                                
 cAAATGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATc                                                                                                 <  1:2511811/97‑1 (MQ=255)
     tGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCaaaa                                                                                             >  2:1543360/1‑97 (MQ=255)
     tGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCaaaa                                                                                             >  1:2341721/1‑97 (MQ=255)
      gCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAACACCGCCATCACAAc                                                                                            >  2:2415398/1‑97 (MQ=255)
        aacaacACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCa                                                                                          <  2:2617615/97‑1 (MQ=255)
        aacaacACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCa                                                                                          <  1:898054/97‑1 (MQ=255)
           aacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCGACTAAAGTAGACGCACCGCCACCAAAACCaccc                                                                                       >  2:1420580/1‑94 (MQ=14)
           aacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTCGAAGCACCGCAATCAAAACCAAAc                                                                                       >  2:3010912/1‑97 (MQ=255)
           aacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCACAACCAAAc                                                                                       >  1:14680/1‑97 (MQ=255)
           aacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAc                                                                                       >  1:2050652/1‑97 (MQ=255)
           aacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAACCACAACCaaca                                                                                       >  2:2695205/1‑95 (MQ=255)
            acacCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcg                                                                                      >  1:1241409/1‑97 (MQ=255)
             cacCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcgc                                                                                     <  2:1401120/97‑1 (MQ=255)
              acCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcgcg                                                                                    >  1:2922945/1‑97 (MQ=255)
              acCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcgcg                                                                                    >  1:2602638/1‑97 (MQ=255)
              acCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcgcg                                                                                    <  1:2163330/97‑1 (MQ=255)
              acCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCAACTAAAGTAGAAGCACCACCACCACAACCAACcgcc                                                                                    >  2:1983776/1‑96 (MQ=14)
               ccATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGa                                                                                   >  1:3411427/1‑97 (MQ=255)
                cATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGac                                                                                  <  1:3137850/97‑1 (MQ=255)
                cATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGac                                                                                  >  1:2273259/1‑97 (MQ=255)
                cATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGac                                                                                  <  2:3401986/97‑1 (MQ=255)
                 aTCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGACGCACCACAACCACAACCAACCGCGaca                                                                                 >  2:1217523/1‑97 (MQ=17)
                 aTCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCAACTAAAGTAGAAACACCGCCACCAAAACCAAACACGaca                                                                                 >  2:2433854/1‑97 (MQ=17)
                  tCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGacaa                                                                                <  1:1189474/97‑1 (MQ=255)
                     tCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAacac                                                                             <  1:2874099/97‑1 (MQ=255)
                       aaCTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCa                                                                           <  1:789376/97‑1 (MQ=255)
                       aaCTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCa                                                                           <  2:2912081/97‑1 (MQ=255)
                        aCTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCAt                                                                          <  2:1669900/97‑1 (MQ=255)
                         cTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCCACCAAAACCAAACGCGACAACACCagc                                                                         >  2:2000179/1‑95 (MQ=255)
                          tAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCt                                                                        <  1:2789939/97‑1 (MQ=255)
                          tAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCt                                                                        <  1:131914/97‑1 (MQ=255)
                          tAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCt                                                                        >  2:3495371/1‑97 (MQ=255)
                           aaaGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCtt                                                                       >  1:2084559/1‑97 (MQ=255)
                            aaGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTc                                                                      <  1:987574/97‑1 (MQ=255)
                              gTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCaa                                                                    <  2:461516/97‑1 (MQ=255)
                              gTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAACCAAAACCACACGCGACAACACCATCTTCaa                                                                    >  2:491966/1‑97 (MQ=255)
                               tGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAAc                                                                   >  2:3432779/1‑97 (MQ=255)
                               tGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCCCCACCAAAAGCAAACACGACAACACCATCCTCAAc                                                                   >  2:2581891/1‑97 (MQ=17)
                                 gAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAAcaa                                                                 >  2:3063284/1‑95 (MQ=255)
                                 gAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTa                                                                 <  2:1520173/97‑1 (MQ=255)
                                 gAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTa                                                                 <  1:3196568/97‑1 (MQ=255)
                                     cACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGt                                                             >  1:1823319/1‑97 (MQ=255)
                                      aCCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTg                                                            >  1:2845938/1‑97 (MQ=255)
                                      aCCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTg                                                            >  2:860293/1‑97 (MQ=255)
                                       ccGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTgg                                                           <  1:2143486/97‑1 (MQ=255)
                                        cGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGa                                                          >  1:2806056/1‑97 (MQ=255)
                                         gCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGaa                                                         >  1:44863/1‑97 (MQ=255)
                                         gCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGaa                                                         >  1:1767982/1‑97 (MQ=255)
                                         gCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGaa                                                         >  2:429891/1‑97 (MQ=255)
                                           aacaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGc                                                       <  2:571997/97‑1 (MQ=255)
                                            acaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCa                                                      <  1:1862092/97‑1 (MQ=255)
                                              aaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCAcc                                                    <  1:1211067/97‑1 (MQ=255)
                                              aaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCAcc                                                    >  2:1697931/1‑97 (MQ=255)
                                               agcagcAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCg                                                   >  1:3020171/1‑97 (MQ=255)
                                                gcagcaAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGc                                                  >  1:1002968/1‑97 (MQ=255)
                                                gcagcaAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGc                                                  <  1:1161404/97‑1 (MQ=255)
                                                gcagcaAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGc                                                  <  1:503581/97‑1 (MQ=255)
                                                gcagcaAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGc                                                  <  2:951534/97‑1 (MQ=255)
                                                     aaaCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaacaa                                             >  1:16275/1‑97 (MQ=255)
                                                       aCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAagc                                           >  2:1324698/1‑97 (MQ=255)
                                                        cgcgACAACGCCATCTTCAACTAAAGTAGCATCACCGCAAACAAAACCACACGCGACAACACCATCAACATCTAAAGTGGAAACACCGCCACAagca                                          >  2:1180598/1‑97 (MQ=18)
                                                        cgcgACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAagca                                          >  2:3185693/1‑97 (MQ=255)
                                                        cgcgACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAagca                                          >  1:699376/1‑97 (MQ=255)
                                                         gcgACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAagcag                                         >  1:1083843/1‑97 (MQ=255)
                                                           gACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAgcagca                                       <  1:336774/97‑1 (MQ=255)
                                                           gACAACACCATCTTCAACTAAAGTAGAAGCACCGCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAgcagca                                       <  2:1924525/97‑1 (MQ=255)
                                                            acaacaCCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCaa                                      <  2:2247392/97‑1 (MQ=255)
                                                             caacaCCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCaaa                                     >  2:2351411/1‑97 (MQ=255)
                                                              aacaCCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAc                                    >  2:1037789/1‑97 (MQ=255)
                                                              aacaCCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAc                                    >  1:2714942/1‑97 (MQ=255)
                                                              aacaCCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAc                                    >  1:2835498/1‑97 (MQ=255)
                                                               acacCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcg                                   >  2:2799330/1‑97 (MQ=255)
                                                                 acCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAACcgcg                                 >  1:1583369/1‑97 (MQ=255)
                                                                 acCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcgcg                                 <  2:1278832/97‑1 (MQ=255)
                                                                   cATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGac                               <  1:1020903/97‑1 (MQ=255)
                                                                   cATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGac                               <  1:104446/97‑1 (MQ=255)
                                                                   cATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGac                               <  1:1667023/97‑1 (MQ=255)
                                                                     ccTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaa                             <  1:387090/96‑1 (MQ=255)
                                                                      cTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaac                            <  1:1291251/97‑1 (MQ=255)
                                                                       ttCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaaca                           >  2:3274227/1‑97 (MQ=255)
                                                                        tCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAacac                          <  1:1981486/97‑1 (MQ=255)
                                                                          aaCTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcacca                        <  2:1102738/97‑1 (MQ=255)
                                                                          aaCTAAAGTAGAAGCACCGCAACCAGAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcacca                        <  2:2279797/97‑1 (MQ=255)
                                                                          aaCTAAAGTAGAAGCACCGCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcacca                        <  2:174482/97‑1 (MQ=255)
                                                                            cTAAAGTAGAAGCACCGCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACaccacc                      <  2:825300/97‑1 (MQ=255)
                                                                             tAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCt                     <  2:1682279/97‑1 (MQ=255)
                                                                               aaGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTc                   >  1:1937804/1‑97 (MQ=255)
                                                                               aaGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTc                   >  2:591477/1‑97 (MQ=255)
                                                                                 gTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGt                 >  2:1800247/1‑97 (MQ=255)
                                                                                   aGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCt               <  1:2777610/97‑1 (MQ=255)
                                                                                   aGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCt               <  1:160163/97‑1 (MQ=255)
                                                                                   aGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCt               <  1:151460/97‑1 (MQ=255)
                                                                                      aGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGCCTAAt            >  1:620080/1‑97 (MQ=255)
                                                                                        cACCGCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGt          <  2:664214/97‑1 (MQ=255)
                                                                                         aCCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTa         <  1:683734/97‑1 (MQ=255)
                                                                                            gCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGac      <  1:471311/97‑1 (MQ=255)
                                                                                            gCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGac      >  1:328056/1‑97 (MQ=255)
                                                                                             caaccacaaccaAACGCGACAACACCAGCTTCAACTAAAGTGGAAGCACCGCAACCAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGaca     <  2:2300677/97‑1 (MQ=18)
                                                                                             cAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGaca     <  2:1467434/97‑1 (MQ=255)
                                                                                              aaTCAAAGCCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGacac    <  1:588553/97‑1 (MQ=255)
                                                                                                |                                                                                                
GCAAATGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGACACAT  >  BX571857/431409‑431601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑