BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BX571857	2,641,524	A→G	8.0%	D114D (GAT→GAC)	SAS2461 ←	putative glyoxalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BX571857	2,641,524	0	A	G	8.0%	1158.3 / 63.6	300	D114D (GAT→GAC)	SAS2461	putative glyoxalase
Reads supporting (aligned to +/- strand): ref base A (151/124); new base G (12/12); total (163/137)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

Only 100 of 306 total aligned reads displayed.

GTGCACATTAAACCTTCATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACT  >  BX571857/2641428‑2641620

gtgCACATTAAACCTTCATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGa                                                                                                  <  2:655829/97‑1 (MQ=255)

gtgCACATTAAACCTTCATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGa                                                                                                  >  2:104381/1‑97 (MQ=255)

gtgCACATTAAACCTTCATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGa                                                                                                  >  2:3062137/1‑97 (MQ=255)

gtgCACATTAAACCTTCATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGa                                                                                                  <  2:2907519/97‑1 (MQ=255)

  gCACATTAAACCTTCATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATc                                                                                                >  1:2455903/1‑97 (MQ=255)

  gCACATTAAACCTTCATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATc                                                                                                <  2:422789/97‑1 (MQ=255)

   cacaTTAAACCTTCATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCt                                                                                               >  2:1010517/1‑97 (MQ=255)

   cacaTTAAACCTTCATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCt                                                                                               >  1:938589/1‑97 (MQ=255)

      aTTAAACCTTCATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTg                                                                                            >  1:23413/1‑97 (MQ=255)

      aTTAAACCTTCATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTg                                                                                            >  2:2652107/1‑97 (MQ=255)

        tAAACCTTCATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGaa                                                                                          <  2:2384649/97‑1 (MQ=255)

         aaaCCTTCATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAAc                                                                                         >  2:1186378/1‑97 (MQ=255)

           aCCTTCATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGGTCTTTGAACAt                                                                                       <  1:2553336/97‑1 (MQ=255)

            ccTTCATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACAtt                                                                                      >  1:3000564/1‑97 (MQ=255)

             cTTCATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGGTCTTTGAACATTg                                                                                     >  2:3417074/1‑97 (MQ=255)

              ttCATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGc                                                                                    <  1:2922420/97‑1 (MQ=255)

              ttCATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGc                                                                                    <  1:2104948/97‑1 (MQ=255)

              ttCATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGc                                                                                    >  1:241502/1‑97 (MQ=255)

                cATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCAc                                                                                  >  1:3281651/1‑97 (MQ=255)

                cATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCAc                                                                                  >  2:1545215/1‑97 (MQ=255)

                cATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCAc                                                                                  <  2:777619/97‑1 (MQ=255)

                     ttGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATaa                                                                             <  2:3434813/97‑1 (MQ=255)

                     ttGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATaa                                                                             <  2:2000899/97‑1 (MQ=255)

                      tGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATaaa                                                                            >  1:3121064/1‑97 (MQ=255)

                      tGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATaaa                                                                            <  2:1648176/97‑1 (MQ=255)

                      tGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATaaa                                                                            <  2:1212022/97‑1 (MQ=255)

                         aTGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGGTCTTTGAACATTGCACCATAAAAcc                                                                         >  1:2855143/1‑97 (MQ=255)

                            ggCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCtt                                                                      <  1:2216493/97‑1 (MQ=255)

                            ggCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCtt                                                                      >  1:1830803/1‑97 (MQ=255)

                             gCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTg                                                                     <  2:35048/97‑1 (MQ=255)

                             gCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTg                                                                     <  1:1148653/97‑1 (MQ=255)

                             gCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTg                                                                     <  2:793056/97‑1 (MQ=255)

                              cTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTgg                                                                    >  2:1560479/1‑97 (MQ=255)

                              cTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTgg                                                                    >  1:1080556/1‑97 (MQ=255)

                              cTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTgg                                                                    >  2:3399865/1‑97 (MQ=255)

                                ttttATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCt                                                                  <  1:2299556/97‑1 (MQ=255)

                                 tttatttaTTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCtt                                                                 >  1:2169032/1‑97 (MQ=255)

                                  ttatttatTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGGTCTTTGAACATTGCACCATAAAACCCTTGGCTTa                                                                >  2:1134986/1‑97 (MQ=255)

                                  ttatttatTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGGTCTTTGAACATTGCACCATAAAACCCTTGGCTTa                                                                <  1:822330/97‑1 (MQ=255)

                                  ttatttatTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTa                                                                <  2:909124/97‑1 (MQ=255)

                                   tatttattCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTAc                                                               >  1:527359/1‑97 (MQ=255)

                                   aatgtattCAGTTGAGAATATCATTCTAGAAGACCAAAAAATTAAAATGGTGTACAACAATATCTTTAAACATTGCACCATAAAACCCTTGGCTTAc                                                               <  2:2053279/93‑1 (MQ=255)

                                    atttattCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACt                                                              >  1:3452555/1‑97 (MQ=255)

                                    atttattCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACt                                                              <  1:1116638/97‑1 (MQ=255)

                                       tattCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGtt                                                           >  1:1134903/1‑97 (MQ=255)

                                       tattCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGtt                                                           >  2:2156778/1‑97 (MQ=255)

                                        attCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGGTCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTg                                                          >  1:3315211/1‑97 (MQ=255)

                                        attCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTg                                                          <  2:1310260/97‑1 (MQ=255)

                                         ttCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTgg                                                         >  1:1280964/1‑97 (MQ=255)

                                          tCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGt                                                        >  2:1960307/1‑97 (MQ=255)

                                          tCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGt                                                        <  2:3272747/97‑1 (MQ=255)

                                          tCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGt                                                        >  2:560686/1‑97 (MQ=255)

                                           cTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGtt                                                       >  1:1980758/1‑97 (MQ=255)

                                           cTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGtt                                                       <  1:192710/97‑1 (MQ=255)

                                            ttttGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTc                                                      <  2:3426472/97‑1 (MQ=255)

                                            ttttGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTc                                                      >  2:1787754/1‑97 (MQ=255)

                                                gagaATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGa                                                  <  2:1114372/97‑1 (MQ=255)

                                                   aaTTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACg                                               <  2:299815/97‑1 (MQ=255)

                                                     tttCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGGTCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGAc                                             <  1:2062717/97‑1 (MQ=255)

                                                     tttCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGAc                                             >  1:3080548/1‑97 (MQ=255)

                                                     tttCAGTTGAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTAAACATTGCACCATAAAACCCTTCGCTTACTGTTGGTTCTTGAACGAc                                             <  2:1155619/97‑1 (MQ=255)

                                                        cATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACtgt                                          <  1:3170481/97‑1 (MQ=255)

                                                        cATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACtgt                                          <  1:3376154/97‑1 (MQ=255)

                                                        cATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACtgt                                          >  1:610984/1‑97 (MQ=255)

                                                           tttAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGcc                                       >  1:437361/1‑97 (MQ=255)

                                                            ttAGCAGCCCAAAAAATTAAAATGGTGTCCATCAAGGTCTTTGCCCATTGCACCATAAACCCCTTGGCTTACTGTTGGTTCTTGAACGCCTGTGCCa                                      <  2:3235728/97‑1 (MQ=255)

                                                            ttAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCa                                      >  2:1608783/1‑97 (MQ=255)

                                                            ttAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCa                                      >  2:2843658/1‑97 (MQ=255)

                                                            ttAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCa                                      >  2:2511077/1‑97 (MQ=255)

                                                                cAGACCAAAAAATTAAAATGGTGTCCATCAAGGTCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCg                                  <  2:386002/97‑1 (MQ=255)

                                                                 aGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGc                                 >  1:2473008/1‑97 (MQ=255)

                                                                 aGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGc                                 <  1:2492308/97‑1 (MQ=255)

                                                                 aGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGc                                 <  1:1694085/97‑1 (MQ=255)

                                                                  gACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGct                                >  1:1306320/1‑97 (MQ=255)

                                                                   aCCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGctt                               <  1:483923/97‑1 (MQ=255)

                                                                    ccAAAAAATTAAAATGGTGTCCATCACGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGcttc                              >  2:2569289/1‑97 (MQ=255)

                                                                    ccAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGcttc                              >  2:913129/1‑97 (MQ=255)

                                                                     cAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGcttct                             <  2:1377598/97‑1 (MQ=255)

                                                                       aaaaaTTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCttt                           >  2:1724043/1‑97 (MQ=255)

                                                                          aaTTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCAc                        <  1:424340/97‑1 (MQ=255)

                                                                          aaTTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCAc                        >  1:1109250/1‑97 (MQ=255)

                                                                           aTTAAGATGGTGTCCATCAAGATCTTTGAGCATTGCACCATAAAGCCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACt                       <  2:60049/97‑1 (MQ=255)

                                                                           aTTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACt                       >  2:229053/1‑97 (MQ=255)

                                                                            ttAAAATGGTGTCCATAAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGCCTGTGCCACACGCTTCTTTTACtt                      >  2:147488/1‑97 (MQ=255)

                                                                              aaaaTGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTa                    >  1:3434923/1‑97 (MQ=255)

                                                                              aaaaTGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTa                    >  1:137667/1‑97 (MQ=255)

                                                                                aaTGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTAtt                  <  1:2732614/97‑1 (MQ=255)

                                                                                aaTGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTAtt                  >  2:3379887/1‑97 (MQ=255)

                                                                                 aTGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTc                 >  2:2403032/1‑97 (MQ=255)

                                                                                   ggTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCAc               >  1:2761061/1‑97 (MQ=255)

                                                                                    gtgtCCATCAAGGTCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCAcc              >  1:2935880/1‑97 (MQ=255)

                                                                                    gtgtCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCAcc              <  2:1894058/97‑1 (MQ=255)

                                                                                    gtgtCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCAcc              >  1:2687473/1‑97 (MQ=255)

                                                                                      gtCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCaa            <  1:1469366/97‑1 (MQ=255)

                                                                                          aTCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTgg        <  1:725964/97‑1 (MQ=255)

                                                                                          aTCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTgg        <  1:567471/97‑1 (MQ=255)

                                                                                            cAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTc      <  1:2255163/97‑1 (MQ=255)

                                                                                            cAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTc      <  1:1048893/97‑1 (MQ=255)

                                                                                               gATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAAc   <  1:1542045/97‑1 (MQ=255)

                                                                                                aTCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAAct  >  2:942741/1‑97 (MQ=255)

GTGCACATTAAACCTTCATCTTTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACT  >  BX571857/2641428‑2641620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`