Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 605,157 T→C 6.0% D1007D (GAT→GAC SAS0521 → bone sialoprotein‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX571857605,1570TC 6.0% 833.9 / 9.0 234D1007D (GAT→GACSAS0521bone sialoprotein‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (104/111);  new base C (13/6);  total (117/117)
Fisher's exact test for biased strand distribution p-value = 1.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

TCAGACTCAGATAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGAC  >  BX571857/605062‑605253
                                                                                               |                                                                                                
tcagactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAtt                                                                                                 <  2:1152073/97‑1 (MQ=40)
 cagactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTc                                                                                                >  2:2699683/1‑97 (MQ=255)
  agactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCa                                                                                               <  1:485260/97‑1 (MQ=255)
  agactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCa                                                                                               <  2:2239646/97‑1 (MQ=255)
    actcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGa                                                                                             <  1:2328393/97‑1 (MQ=255)
     ctcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGAc                                                                                            <  1:257283/97‑1 (MQ=255)
      tcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACa                                                                                           >  2:2468450/1‑97 (MQ=255)
         gaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCg                                                                                        <  2:2927168/97‑1 (MQ=40)
         gaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCg                                                                                        <  1:176119/97‑1 (MQ=255)
         gaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCg                                                                                        <  1:2433279/97‑1 (MQ=40)
          aTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                                                                                       <  2:283744/97‑1 (MQ=39)
           tAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                                                                      >  2:1369838/1‑97 (MQ=255)
           tAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAt                                                                                      >  2:2140835/1‑97 (MQ=255)
             gTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTc                                                                                    >  1:2418542/1‑97 (MQ=255)
               gattcagattcaGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                  <  1:8510/97‑1 (MQ=40)
               gattcagattcaGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                  <  1:3090073/97‑1 (MQ=255)
                attcagattcagATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa                                                                                 <  1:2803658/97‑1 (MQ=255)
                    agattcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCa                                                                             <  2:2646727/97‑1 (MQ=255)
                     ggttcggatAGCGACTCAGCCTCAGATGGCGGCTCAGGTTCGGACAGCGAGTCAGGCTCAGATAGCGGCTCAGATTCAGACAGCGATTCAGACTCAg                                                                            <  2:1889324/95‑1 (MQ=14)
                       ttcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                                                                          <  1:2071565/97‑1 (MQ=255)
                       ttcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                                                                          >  1:2790985/1‑97 (MQ=40)
                        tcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATa                                                                         >  1:3253526/1‑97 (MQ=255)
                        tcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATa                                                                         >  1:602267/1‑97 (MQ=17)
                         cagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                                                        >  2:2304869/1‑97 (MQ=40)
                         cagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAg                                                                        >  2:3276761/1‑97 (MQ=21)
                         cagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACCCAGACAGCGATTCAGACTAAGATAg                                                                        >  2:3234406/1‑97 (MQ=255)
                           gatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCg                                                                      >  1:1001863/1‑97 (MQ=255)
                           gatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCg                                                                      <  2:2604892/97‑1 (MQ=255)
                            atAGCGACTCAGCCTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGa                                                                     <  2:2886022/97‑1 (MQ=255)
                             tAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGAc                                                                    <  1:34188/97‑1 (MQ=255)
                              aGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACt                                                                   <  1:707818/97‑1 (MQ=255)
                               gCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTc                                                                  >  1:3204049/1‑97 (MQ=255)
                                cGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCa                                                                 <  1:2756057/97‑1 (MQ=255)
                                cGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCa                                                                 >  1:1507974/1‑97 (MQ=255)
                                 gactcagactcaGATAGCGACTCCGATTCGGACAGCGATTCAGACTCACATAGCGACTCCGATTCACACAGAGACTCAGACTCAGATAGCGCCACAg                                                                >  2:1488453/1‑97 (MQ=11)
                                   ctcagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGac                                                              >  2:2492619/1‑96 (MQ=255)
                                   ctcagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGac                                                              >  2:965715/1‑96 (MQ=255)
                                   ctcagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAt                                                              <  1:1581170/97‑1 (MQ=255)
                                    tcagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGagt                                                             >  2:221064/1‑95 (MQ=255)
                                    tcagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAtt                                                             >  1:2623984/1‑97 (MQ=40)
                                    tcagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGAtt                                                             >  2:2418742/1‑97 (MQ=255)
                                      agactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactca                                                           >  2:1535998/1‑97 (MQ=255)
                                         ctcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGAc                                                        <  1:888252/97‑1 (MQ=255)
                                          tcagaCAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACa                                                       <  2:2406698/97‑1 (MQ=255)
                                           cagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAg                                                      >  2:3136014/1‑97 (MQ=255)
                                           cagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAg                                                      >  2:1197721/1‑97 (MQ=255)
                                              aTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTga                                                   <  1:2998841/97‑1 (MQ=255)
                                               tAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCCGACTCAGATAGCGACTCAGACTCAGACAGTgac                                                  >  2:3447022/1‑97 (MQ=255)
                                               tAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgac                                                  >  1:1370051/1‑97 (MQ=255)
                                               caGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGAGTCAGATTGAGGCAgagag                                                  >  2:3382764/2‑96 (MQ=11)
                                                aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgact                                                 <  2:2345869/97‑1 (MQ=255)
                                                aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgacc                                                 >  1:2636442/1‑96 (MQ=255)
                                                 gCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGa                                                                     >  2:365479/1‑76 (MQ=255)
                                                   gACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcag                                              >  1:2727165/1‑97 (MQ=255)
                                                   gACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcag                                              <  1:3194857/97‑1 (MQ=255)
                                                      tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcagact                                           <  1:1976191/97‑1 (MQ=255)
                                                       cAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcagactc                                          >  1:3480610/1‑97 (MQ=255)
                                                       cAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcagactc                                          >  1:84046/1‑97 (MQ=255)
                                                          aTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGActcagactcaga                                       <  1:1724933/97‑1 (MQ=255)
                                                           ttCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGAt                                      >  2:2956158/1‑97 (MQ=255)
                                                           ttCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGAt                                      <  1:1092936/97‑1 (MQ=255)
                                                           ttCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGAt                                      >  2:108477/1‑97 (MQ=255)
                                                            tCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATa                                     <  1:1328600/97‑1 (MQ=255)
                                                               gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCg                                  <  1:423633/97‑1 (MQ=255)
                                                                 cAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgac                                <  1:274408/97‑1 (MQ=255)
                                                                 cAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgac                                >  2:606322/1‑97 (MQ=255)
                                                                 cAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgac                                <  1:3383026/97‑1 (MQ=255)
                                                                  aGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgact                               >  1:3301894/1‑97 (MQ=255)
                                                                  aGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgact                               <  2:1653257/97‑1 (MQ=255)
                                                                  aGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGATAGCgact                               >  2:3004456/1‑97 (MQ=255)
                                                                   gCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactc                              >  1:1913723/1‑97 (MQ=255)
                                                                    cGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactca                             <  1:2258306/97‑1 (MQ=255)
                                                                     gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcag                            >  2:1399006/1‑97 (MQ=255)
                                                                     gATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcag                            <  2:653526/97‑1 (MQ=255)
                                                                      aTTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcaga                           >  2:325214/1‑97 (MQ=255)
                                                                            aCTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGActcagactcaga                     >  2:2163752/1‑97 (MQ=255)
                                                                            aCTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGActcagactcaga                     <  2:1832759/97‑1 (MQ=255)
                                                                             cTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACtcagactcagac                    <  2:383282/97‑1 (MQ=255)
                                                                              tCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACa                   <  1:2447632/97‑1 (MQ=255)
                                                                               cAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAg                  >  1:2915770/1‑97 (MQ=255)
                                                                                aGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGt                 >  1:392173/1‑97 (MQ=255)
                                                                                 gacaGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                <  2:1947759/94‑1 (MQ=11)
                                                                                  aTAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGGCTCAGATAGCGACTCAGACTCAGACAGTga               >  2:515499/1‑97 (MQ=255)
                                                                                  aTAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTga               >  2:3421084/1‑97 (MQ=255)
                                                                                     gCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactc            >  1:3468227/1‑97 (MQ=255)
                                                                                     gCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactc            >  2:3041619/1‑97 (MQ=255)
                                                                                      cGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactca           <  2:25437/97‑1 (MQ=255)
                                                                                       gACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcag          >  2:19398/1‑97 (MQ=255)
                                                                                       gACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcag          <  2:808376/97‑1 (MQ=255)
                                                                                       gACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcag          >  1:2123329/1‑97 (MQ=255)
                                                                                          tCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagact       >  1:1860875/1‑97 (MQ=255)
                                                                                          tCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagact       <  1:2189174/97‑1 (MQ=255)
                                                                                          tCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagact       >  1:2736065/1‑97 (MQ=255)
                                                                                            agcttCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagactca     <  2:1693462/94‑1 (MQ=255)
                                                                                            aGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGCCTCAGATAGCGACTCAGACTCAGACAGTgactcagactca     >  2:3493645/1‑97 (MQ=255)
                                                                                            aGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagactca     <  1:938843/97‑1 (MQ=255)
                                                                                            aGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagactca     >  2:1983026/1‑97 (MQ=255)
                                                                                            aGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagactca     <  2:700195/97‑1 (MQ=255)
                                                                                             gATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGactcagactcag    <  1:1238204/97‑1 (MQ=255)
                                                                                               ttCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACtcagactcagac  >  2:2783070/1‑97 (MQ=255)
                                                                                               |                                                                                                
TCAGACTCAGATAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGAC  >  BX571857/605062‑605253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: