Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 431,505 T→C 12.1% S77P (TCA→CCA)  SAS0387 → exotoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX571857431,5050TC12.1% 522.0 / 15.8 157S77P (TCA→CCA) SAS0387exotoxin
Reads supporting (aligned to +/- strand):  ref base T (63/72);  new base C (9/12);  total (72/85)
Fisher's exact test for biased strand distribution p-value = 8.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 160 total aligned reads displayed.

GCAAATGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGACACAT  >  BX571857/431409‑431601
                                                                                                |                                                                                                
 cAAATGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATc                                                                                                 >  1:1615554/1‑97 (MQ=255)
  aaaTGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCa                                                                                                >  1:1519303/1‑97 (MQ=255)
  aaaTGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCa                                                                                                >  1:2143013/1‑97 (MQ=255)
    aTGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCaaa                                                                                              >  1:1485382/1‑97 (MQ=255)
    aTGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCaaa                                                                                              <  2:1862740/97‑1 (MQ=255)
     tGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCaaaa                                                                                             >  1:2223609/1‑97 (MQ=255)
     tGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAACCaaaa                                                                                             >  2:798859/1‑97 (MQ=255)
         acaacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCaa                                                                                         <  1:483949/97‑1 (MQ=255)
         acaacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCaa                                                                                         <  2:1186798/97‑1 (MQ=255)
          caacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCaaa                                                                                        >  1:1244011/1‑97 (MQ=255)
          caacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCaaa                                                                                        >  1:1244022/1‑97 (MQ=255)
            acacCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGACGCACCGCAACCACAACCAACcg                                                                                      >  2:500379/1‑97 (MQ=17)
             cacCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcgc                                                                                     <  1:730453/97‑1 (MQ=255)
             cacCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcgc                                                                                     <  2:2189251/97‑1 (MQ=255)
             cacCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcgc                                                                                     <  2:1055611/97‑1 (MQ=255)
              acCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcgcg                                                                                    <  1:1831339/97‑1 (MQ=255)
                cATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGac                                                                                  >  1:696105/1‑97 (MQ=255)
                cATCTTCAACCATAGTGGAAGCACCGCAACAAGGAGCAAGCGCGACAACCCCACCATCAACTAAAGTAGAAGCCCCGCAATCAAAACCAAAcgcggc                                                                                  <  2:1879936/97‑3 (MQ=18)
                 aTCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAACCAACACCAAACGCGaca                                                                                 >  2:840440/1‑97 (MQ=255)
                 aTCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAACACCGCAACCAAAACCAAACGCGaca                                                                                 >  2:2084535/1‑97 (MQ=255)
                  tCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGacaa                                                                                <  2:1525440/97‑1 (MQ=255)
                    ttCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGacaaca                                                                              >  1:1904744/1‑97 (MQ=255)
                    ttCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGacaaca                                                                              >  1:1150268/1‑97 (MQ=255)
                     tCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAacac                                                                             <  2:518708/97‑1 (MQ=255)
                       aaCTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCa                                                                           <  1:1660277/97‑1 (MQ=255)
                         cTAAAGTGGAAGCACCGCAACGAGCAGCACCCGCGACATCACCCTCCTCAACTAAGGTAGAAGCACCGCCATCAAAACCAAACGCGACAACACCATc                                                                         <  2:270215/97‑1 (MQ=21)
                         cTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGACGCACCGCAACCAAAACCAAACGCGACAACACCATc                                                                         >  2:573990/1‑97 (MQ=255)
                         cTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATc                                                                         <  1:1994348/97‑1 (MQ=255)
                           aaaGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCtt                                                                       >  1:1296124/1‑97 (MQ=255)
                            aaGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAACCGCGACAACACCATCTTc                                                                      <  2:619767/97‑1 (MQ=255)
                             aGTGGAAGCACCGCAACAAGCAGCACACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCa                                                                     <  1:1004057/97‑1 (MQ=255)
                             aGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCa                                                                     <  2:408886/97‑1 (MQ=255)
                               tGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAAc                                                                   >  2:706554/1‑97 (MQ=255)
                                ggAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACt                                                                  >  2:1160724/1‑97 (MQ=255)
                                ggAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACt                                                                  >  1:1158288/1‑97 (MQ=255)
                                   aGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTaaa                                                               >  1:1743221/1‑97 (MQ=255)
                                   aGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTaaa                                                               <  2:595517/97‑1 (MQ=255)
                                   aGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCATCAACTaaa                                                               >  2:1675350/1‑97 (MQ=255)
                                      aCCGGAACAAGCAGCAAACGCGACAACACCATCTCCAACTAAAGTAGAAGCACCGCAATCCAAACCAAACGCGACAAAACAATCTTCAACTAAAGTg                                                            >  2:336833/1‑97 (MQ=21)
                                      aCCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTg                                                            >  2:1449881/1‑97 (MQ=255)
                                       ccGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTgg                                                           <  1:1700494/97‑1 (MQ=255)
                                        cGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGa                                                          <  1:64267/97‑1 (MQ=255)
                                           aacaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGc                                                       <  2:225630/97‑1 (MQ=255)
                                           aacaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGc                                                       <  2:1554579/97‑1 (MQ=255)
                                           aacaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGc                                                       <  1:1682248/97‑1 (MQ=255)
                                           aacaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGc                                                       <  1:1131361/97‑1 (MQ=255)
                                            acaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCa                                                      <  1:694805/97‑1 (MQ=255)
                                            acaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCa                                                      >  1:2174826/1‑97 (MQ=255)
                                             caaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCAc                                                     >  2:1989248/1‑97 (MQ=255)
                                             caaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCAc                                                     >  1:85444/1‑97 (MQ=255)
                                              aaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCAcc                                                    >  2:1782936/1‑97 (MQ=255)
                                              aaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCAcc                                                    >  2:1782727/1‑97 (MQ=255)
                                                gcagcaAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGc                                                  >  2:2265551/1‑97 (MQ=255)
                                                   gcaAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaac                                               <  2:2244033/97‑1 (MQ=255)
                                                   gcaAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaac                                               <  2:975824/97‑1 (MQ=255)
                                                     aaaCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaacaa                                             >  1:786466/1‑97 (MQ=255)
                                                     aaaCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaacaa                                             >  1:1017617/1‑97 (MQ=255)
                                                     aaaCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaacaa                                             <  2:87767/97‑1 (MQ=255)
                                                     aaaCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaacaa                                             >  1:453949/1‑97 (MQ=255)
                                                      aaCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAag                                            <  2:1483261/97‑1 (MQ=255)
                                                      aaCGCGACAAAACAATAATAAACTAAAAAAGAAAAAACGCAATAAAAACCAAACGAGAAAACACCAACTTCAACTAAAGAGGAAGCACCGCAACAag                                            >  2:1621912/1‑97 (MQ=12)
                                                       aCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAagc                                           <  1:2134343/97‑1 (MQ=255)
                                                       aCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAagc                                           >  2:1176798/1‑97 (MQ=255)
                                                           gACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAgcagca                                       <  1:955159/97‑1 (MQ=255)
                                                             caacaCCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCaaa                                     >  1:1075542/1‑97 (MQ=255)
                                                              aacaCCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAc                                    >  1:194939/1‑97 (MQ=255)
                                                              aacaCCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAc                                    >  1:527509/1‑97 (MQ=255)
                                                                 acCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcgcg                                 >  1:564514/1‑97 (MQ=255)
                                                                 acCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcgcg                                 >  2:12259/1‑97 (MQ=255)
                                                                   cATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGac                               >  2:148257/1‑97 (MQ=255)
                                                                   cATCTTCAACTAAAGTAGAAGCACCGCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGac                               <  2:1194403/97‑1 (MQ=255)
                                                                    aTCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCACACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGaca                              >  2:516661/1‑97 (MQ=255)
                                                                     tCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaa                             >  2:1060475/1‑97 (MQ=255)
                                                                      cTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaac                            >  2:139057/1‑97 (MQ=255)
                                                                      cTTCAACTAAAGTAGAAGCACCGCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaac                            <  2:173290/97‑1 (MQ=255)
                                                                       ttCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaaca                           >  1:850601/1‑97 (MQ=255)
                                                                       ttCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaaca                           >  1:850494/1‑97 (MQ=255)
                                                                        tCAACTAAAGTAGAAGCACCGCAATCAGAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAacac                          <  2:1218720/97‑1 (MQ=255)
                                                                        tCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAacac                          <  1:2039193/97‑1 (MQ=255)
                                                                        tCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAacac                          >  2:1538006/1‑97 (MQ=255)
                                                                          aaCTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcacca                        <  2:1309961/97‑1 (MQ=255)
                                                                          aaCTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcacca                        <  1:2267526/97‑1 (MQ=255)
                                                                           aCTAAAGTAGAAGCACCGCAATCAGAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcaccac                       <  2:847010/97‑1 (MQ=255)
                                                                           aCTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcaccac                       <  1:1070226/97‑1 (MQ=255)
                                                                             tAAAGTAGAAGCACCGCAAACAAAACCAAACGCAACAACACCATCTTCCACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCt                     <  1:756951/97‑1 (MQ=255)
                                                                               aaGTAGAAGCACCGCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTc                   <  2:234921/97‑1 (MQ=255)
                                                                                aGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCg                  <  1:1317200/97‑1 (MQ=255)
                                                                                aGTAGAAGCACCGCAACCAAAACCAAACGCGACAACCCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCg                  <  1:1349894/97‑1 (MQ=255)
                                                                                    gAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTa              >  2:135725/1‑97 (MQ=255)
                                                                                    gAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTa              >  1:1568195/1‑97 (MQ=255)
                                                                                    gAAGCACCGCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTa              <  1:1489686/97‑1 (MQ=255)
                                                                                     aaGCACCGCAATCAGAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTaa             <  2:1598251/97‑1 (MQ=255)
                                                                                        cACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGt          >  1:917857/1‑97 (MQ=255)
                                                                                         aCCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTa         >  2:1092585/1‑97 (MQ=255)
                                                                                         aCCGCAACCAAAACCAAACGCGACAACACCATCTTCAACGAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTa         <  2:1805874/97‑1 (MQ=255)
                                                                                             cAATCAGTACCAAACGCGACAACGCCATCGTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGaca     <  2:1695593/97‑1 (MQ=18)
                                                                                             cAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGaca     <  1:173072/97‑1 (MQ=255)
                                                                                             cAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGaca     <  2:698997/97‑1 (MQ=255)
                                                                                               aTCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGAcaca   <  2:899660/97‑1 (MQ=255)
                                                                                                tCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGACACAt  >  2:1991046/1‑97 (MQ=255)
                                                                                                |                                                                                                
GCAAATGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGACACAT  >  BX571857/431409‑431601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑