Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 2,700,209 A→G 5.4% D753D (GAT→GAC SAS2516 ← fibrinogen and keratin‑10 binding surface anchored protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX5718572,700,2090AG 5.4% 949.8 / 11.8 263D753D (GAT→GACSAS2516fibrinogen and keratin‑10 binding surface anchored protein
Reads supporting (aligned to +/- strand):  ref base A (93/145);  new base G (5/13);  total (101/162)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

GTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTA  >  BX571857/2700113‑2700305
                                                                                                |                                                                                                
gtctgagtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGaa                                                                                                  <  1:2133906/97‑1 (MQ=255)
  ctgagtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGACTc                                                                                                >  2:817244/1‑97 (MQ=255)
  ctgagtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATc                                                                                                >  1:1753814/1‑97 (MQ=255)
  ctgagtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCgctgtctgagtctgaatcgctgtctgagtctgaatc                                                                                                >  2:720493/1‑97 (MQ=255)
   tgagtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCg                                                                                               <  2:631253/97‑1 (MQ=255)
   tgagtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCg                                                                                               >  1:2168435/1‑97 (MQ=255)
            atctgaatctgaATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctgaa                                                                                      <  2:1152901/97‑1 (MQ=255)
            atctgaatctgaATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctgaa                                                                                      >  1:1255006/1‑97 (MQ=255)
             tctgaatctgaaTCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGtctgaat                                                                                     >  2:1222880/1‑97 (MQ=255)
             tctgaatctgaaTCGCTGTCTGAGTCTGAATCACTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGGCTGAGCCTGACTCGCTGtctgaaa                                                                                     >  2:2195018/1‑96 (MQ=14)
                gaatctgaatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctgaatctg                                                                                  <  2:1053055/97‑1 (MQ=255)
                     tgaatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGctgtctgaatctgaatcgctgtctgaatctgaatcg                                                                             >  2:628959/1‑97 (MQ=255)
                      gagtcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGc                                                                            <  2:1023322/94‑1 (MQ=255)
                         tcGCTGTCGGAGTCTGACTCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgt                                                                         <  2:1643986/97‑1 (MQ=255)
                          cGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtc                                                                        >  1:792452/1‑97 (MQ=255)
                          cGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtc                                                                        >  1:1930519/1‑97 (MQ=255)
                           gCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtct                                                                       >  2:1688369/1‑97 (MQ=255)
                           gCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtct                                                                       >  2:326865/1‑97 (MQ=255)
                           gCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtct                                                                       <  2:2066565/97‑1 (MQ=255)
                            ccgtctgagtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtctg                                                                      <  2:155152/95‑1 (MQ=255)
                            cTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtctg                                                                      <  1:28754/97‑1 (MQ=255)
                            cTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtctg                                                                      <  1:120104/97‑1 (MQ=255)
                             tGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTgtctga                                                                     >  2:2211319/1‑97 (MQ=255)
                             tGTCGGTGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGGTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTgtctga                                                                     <  2:1335091/97‑1 (MQ=17)
                              gtctgagtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGATTCTGAATCGCTgtctgag                                                                    >  1:525832/1‑97 (MQ=255)
                              gtctgagtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtctgag                                                                    >  1:384501/1‑97 (MQ=255)
                               tctgagtctgaCTCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtctgagt                                                                   <  2:45423/97‑1 (MQ=255)
                               tctgagtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCCGAATCGCTGTCTGAATCTGATTCGCTgtctgaat                                                                   >  2:1184638/1‑95 (MQ=255)
                                ctgagtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtctgagtc                                                                  >  2:84015/1‑97 (MQ=255)
                                  gagtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtctgagtctg                                                                >  1:534462/1‑97 (MQ=255)
                                   agtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtctgagtctga                                                               <  1:2153826/97‑1 (MQ=255)
                                   agtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtctgagtctga                                                               <  2:1441231/97‑1 (MQ=255)
                                      ctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGACTCGCTGTCTGAGTCTGAATc                                                            >  2:1765208/1‑97 (MQ=255)
                                      ctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATc                                                            <  1:634342/97‑1 (MQ=255)
                                        gaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGc                                                          <  1:1824445/97‑1 (MQ=255)
                                           tCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTAt                                                       >  2:504500/1‑97 (MQ=255)
                                           tCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAt                                                       <  1:10445/97‑1 (MQ=255)
                                              ctggctgagtctgagtcgctgtctgagtctgagtcgctgtCTGAGGCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAATCGCTAtctg                                                    <  2:54427/93‑1 (MQ=11)
                                               tATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctga                                                   <  2:651809/97‑1 (MQ=255)
                                                aTCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctgag                                                  <  2:1521943/97‑1 (MQ=11)
                                                aTCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctgag                                                  <  1:214522/97‑1 (MQ=255)
                                                 tCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTctgagtcgctgtctgagtctgagtcgctgtctgagt                                                 >  2:410290/1‑97 (MQ=14)
                                                   tgagtctgagtcgctgtctgagtctgagtcgctgtcTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctgagtct                                               <  2:2162491/97‑1 (MQ=255)
                                                   tGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctgagtct                                               >  1:608595/1‑97 (MQ=255)
                                                   tGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctgagtct                                               >  1:1567858/1‑97 (MQ=255)
                                                   tGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCCGAATCGCta                                                                          >  1:6546/1‑69 (MQ=255)
                                                    gagtctgagtcgctgtctgagtctgagtcgctgtctgagTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctgagtctg                                              <  1:1699997/94‑1 (MQ=255)
                                                    gATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctgagtctg                                              <  1:1686071/97‑1 (MQ=255)
                                                       tctgagtcgctgtctgagtctgagtcgctgtctgagTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAGTCGCTATctgagtctgagt                                           >  2:710436/1‑97 (MQ=255)
                                                       tctgagtcgctgtctgagtctgagtcgctgtctgagTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATctgagtctgagt                                           >  1:371967/1‑97 (MQ=255)
                                                       tctgagtcgctgtctgagtctgagtcgctgtctgagTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATctgagtctgagt                                           >  2:1826437/1‑97 (MQ=255)
                                                       tctgagtcgctgtcggagtctgagtcgctgtctgagTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATctgagtctgagt                                           <  2:462176/97‑1 (MQ=255)
                                                        ctgagtcgctgtctgagtctgagtcgctgtctgagtCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCtgagtctgagtc                                          <  2:668808/97‑1 (MQ=255)
                                                          gagtcgctgtctgagtctgagtcgctgtctggggctGAATCGCTGTCTGAATCTGAATCGCCGGCTGAGTCTGAATCGCGATCTGTGTCTGAGTCAc                                        <  2:1587453/97‑1 (MQ=17)
                                                          gagtcgctgtctgagtctgagtcgctgtctgagtctGAATCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCAc                                        <  1:1303139/97‑1 (MQ=255)
                                                           agtcgctgtctgagtctgagtcgctgtctgagtctgAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACt                                       <  2:425667/97‑1 (MQ=255)
                                                            gtcgctgtctgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTg                                      <  1:796830/97‑1 (MQ=255)
                                                             tcgctgtctgagtctgagtcgctgtctgagtccgaATCGCTGTCTGAATCCGAATCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTGt                                     >  2:312546/1‑97 (MQ=14)
                                                               gctgtctgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCt                                                                           >  1:736898/1‑57 (MQ=255)
                                                                ctgtctgattctgagtctctgtctgcgtctgaCTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCCGAGTCACTATCTg                                  <  2:483182/97‑1 (MQ=11)
                                                                ctgtctgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGTTATCTGAGTCTGAGTCACTGTCTg                                  <  2:1858102/97‑1 (MQ=255)
                                                                ctgtctgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTg                                  <  1:141753/97‑1 (MQ=255)
                                                                 tgtctgagtctgagtcgctgtctgagtctgaATCGCTGtctgag                                                                                      >  1:21256/1‑43 (MQ=17)
                                                                  gtctgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGaa                                >  1:1426814/1‑97 (MQ=255)
                                                                  gtctgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGaa                                <  2:842136/97‑1 (MQ=255)
                                                                  gtctgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCCGAGTCACTATCTGaa                                >  1:881024/1‑97 (MQ=255)
                                                                   tctgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAAt                               <  2:2048567/97‑1 (MQ=255)
                                                                    ctgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATc                              >  2:2012739/1‑97 (MQ=255)
                                                                     tgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCt                             >  2:1488887/1‑97 (MQ=255)
                                                                     cgagtctgagtcgctgtctgagtctgaATCGCTGtctgaatc                                                                                    >  2:286817/2‑42 (MQ=38)
                                                                      gagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTg                            <  1:606052/97‑1 (MQ=255)
                                                                          ctgagtcgctgtctgagtctgaGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAATCGCTATCTGAATCTGACTc                        <  2:58649/97‑1 (MQ=11)
                                                                          ctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTc                        >  1:704646/1‑97 (MQ=255)
                                                                            gagtcgctgtctgggtctgaATCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCAc                      <  2:785614/97‑1 (MQ=255)
                                                                              gtcgctgtctgagtctgaGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTa                    <  2:1127347/97‑1 (MQ=255)
                                                                                cgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatc                  >  1:563722/1‑97 (MQ=255)
                                                                                cgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAAGCTGACTCACTatc                  >  2:1418566/1‑97 (MQ=255)
                                                                                cgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTATCTGAATCTGACTCACTatc                  >  2:938354/1‑97 (MQ=255)
                                                                                  ctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctg                <  2:262618/97‑1 (MQ=255)
                                                                                   tgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctga               <  2:212033/97‑1 (MQ=255)
                                                                                    gtctgagtctgaGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctgaa              <  2:1655003/97‑1 (MQ=255)
                                                                                    gtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctgaa              <  1:1887294/97‑1 (MQ=255)
                                                                                    gtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctgaa              <  2:1967676/97‑1 (MQ=255)
                                                                                      ctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctgaatc            >  1:1350577/1‑97 (MQ=255)
                                                                                       tgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctgaatct           <  1:870453/97‑1 (MQ=255)
                                                                                       tgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctgaatct           <  2:1926172/97‑1 (MQ=255)
                                                                                       tgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctgaatct           <  2:21433/97‑1 (MQ=255)
                                                                                        gagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctgaatctg          <  1:1175347/97‑1 (MQ=255)
                                                                                         agtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctgaatctga         <  1:2093222/97‑1 (MQ=255)
                                                                                         agtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctgaatctga         <  1:1095125/97‑1 (MQ=255)
                                                                                         agtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctgaatctga         <  2:918394/97‑1 (MQ=255)
                                                                                         agtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAATCTGACTCGCTATCTGAGTCTGAGTCGCTGTCTGCATCTGACTCCCTatctggctctgg         >  2:1626562/1‑96 (MQ=14)
                                                                                           tctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGt       >  1:1282295/1‑97 (MQ=255)
                                                                                            ctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctga                                                   >  1:848388/1‑52 (MQ=38)
                                                                                            ctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTc      >  1:1190669/1‑97 (MQ=255)
                                                                                            ctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTc      >  1:204901/1‑97 (MQ=255)
                                                                                             tgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCg     >  1:1000913/1‑97 (MQ=255)
                                                                                              gaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGc    >  1:272925/1‑97 (MQ=255)
                                                                                              gaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGc    <  1:2016542/97‑1 (MQ=255)
                                                                                                aTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTa  <  1:1537664/97‑1 (MQ=39)
                                                                                                |                                                                                                
GTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTA  >  BX571857/2700113‑2700305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: