| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BX571858 | 20,628:1 | +G | 100% | coding (288/306 nt) | pSAS24 → | putative exported protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BX571858 | 20,628 | 1 | . | G | 100.0% | 160.9 / NA | 38 | K96K (AAA→AAG) | pSAS24 | putative exported protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (16/22); total (16/22) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATTGGATTTTTTAGGTTATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAA‑ATATTCCTAGCTTTTTAAAATGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑G > BX571858/20538‑20652 | aTTGGATTTTTTAGGTTATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTc < 1:189731/98‑1 (MQ=255) ttttttAGGTTATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCt < 1:127203/98‑1 (MQ=255) ggTTATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTaaa < 2:166979/98‑1 (MQ=255) ttATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAAt < 1:251758/98‑1 (MQ=255) aTGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGa > 1:141800/1‑98 (MQ=255) tCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtg < 1:91208‑M2/98‑5 (MQ=255) ccTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtga > 2:161036‑M2/1‑93 (MQ=255) aCAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccg < 2:6448‑M2/98‑9 (MQ=255) cAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccga < 2:219265‑M2/98‑10 (MQ=255) cAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccga < 1:85638‑M2/98‑10 (MQ=255) aaTATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtga < 2:219211‑M2/98‑16 (MQ=255) gtgAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaa > 1:59388‑M2/1‑78 (MQ=255) tAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaa > 2:298675‑M2/1‑71 (MQ=255) tAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaa < 2:282287‑M2/98‑28 (MQ=255) aaaTCATAAAGGGAAGTCTGTGCGATCGATAGAATACATTACAAAAGATATTCCTGGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaa < 2:118765‑M2/98‑29 (MQ=255) aaTCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaat < 2:249191‑M2/98‑30 (MQ=255) aTCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatg > 1:320384‑M2/1‑68 (MQ=255) cATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtt > 2:89425‑M2/1‑66 (MQ=255) tAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttt < 2:151337‑M2/98‑35 (MQ=255) gggAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgta > 2:237584‑M2/1‑60 (MQ=255) ggAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaa > 2:171856‑M2/1‑59 (MQ=255) aaaTATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaa < 2:319640‑M2/98‑42 (MQ=255) atatGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaa > 1:125589‑M2/1‑55 (MQ=255) atatGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaa < 1:3394‑M2/98‑44 (MQ=255) tatGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaa < 2:194765‑M2/98‑45 (MQ=255) aatgTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaagcttgaaaatgtttttgtaaaaaag > 2:148205‑M2/2‑54 (MQ=255) tgCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggta < 1:296786‑M2/98‑49 (MQ=255) ttCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaataa < 2:274857‑M2/98‑53 (MQ=255) tCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaataaa < 1:236021‑M2/98‑54 (MQ=255) tCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaataaa < 2:8349‑M2/98‑54 (MQ=255) tCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaataaa < 2:206946‑M2/98‑54 (MQ=255) cGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaataaaa > 1:157254‑M2/1‑44 (MQ=255) tAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaataaaaaca > 2:130961‑M2/1‑41 (MQ=255) aGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaataaaaacat > 1:163013‑M2/1‑40 (MQ=255) gAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaataaaaacatt > 1:205374‑M2/1‑39 (MQ=255) gAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaaaattttaaaaaaaaaaggtaataaaaacatt > 2:121907‑M2/1‑39 (MQ=255) aCATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaataaaaacattttag > 2:141800‑M2/1‑35 (MQ=255) tACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaataaaaacattttagacgg < 2:69101‑M2/98‑68 (MQ=255) aaaag‑atATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaataaaaacattttagacggatg < 1:205312‑M2/97‑71 (MQ=255) a‑aTATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaataaaaacattttagacggatgtaac > 2:166835‑M2/1‑24 (MQ=255) | ATTGGATTTTTTAGGTTATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAA‑ATATTCCTAGCTTTTTAAAATGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑G > BX571858/20538‑20652 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |