Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 1,252,438 T→C 100% intergenic (+358/‑77) SAS1171 → / → SAS1172 signal recognition particle protein/30S ribosomal protein S16

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX5718571,252,4380TC100.0% 43.0 / NA 11intergenic (+358/‑77)SAS1171/SAS1172signal recognition particle protein/30S ribosomal protein S16
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/8);  total (3/8)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TATAACCTATTAACAGTCTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTATAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGATTTT  >  BX571857/1252346‑1252510
                                                                                            |                                                                        
tataACCTATTAACAGTCTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACa                                                                     <  1:188695/98‑1 (MQ=255)
      cTATTAACAGTCTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTcaca                                                               <  1:166244/98‑1 (MQ=255)
                          tAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCt                                           <  2:317386/98‑1 (MQ=255)
                                 aTAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCCCACACCTGTTACTATTATTTCTTGTAGaa                                    >  2:57726/1‑98 (MQ=255)
                                   atttattaTTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGaaaa                                  <  2:159291/96‑1 (MQ=255)
                                   aGTTATTATTGTAAGAAAAATGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGaaaa                                  <  2:159421/98‑1 (MQ=255)
                                          attGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAAt                           >  1:246228/1‑98 (MQ=255)
                                                            aataatTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAgg         <  1:188521/98‑1 (MQ=255)
                                                              taatTTTCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGgag       <  2:290251/98‑1 (MQ=255)
                                                                  tttCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGAttt   <  1:71143/98‑1 (MQ=255)
                                                                   ttCCTGGTAAAGAAAAAACTCTTTACAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGAtttt  >  1:325726/1‑98 (MQ=255)
                                                                                            |                                                                        
TATAACCTATTAACAGTCTAATTTAGTAATCACATAGTTATTATTGTAAGAAAAAGGTTCAATAATTTTCCTGGTAAAGAAAAAACTCTTTATAAACATTCACACACCTGTTAATATTATTTCTTGTAGAAAATAAAAATTAAAACATGACTTAAAGGAGATTTT  >  BX571857/1252346‑1252510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑