| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BX571857 | 1,868,342 | G→A | 100% | G129D (GGT→GAT) | SAS1720 → | putative membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BX571857 | 1,868,342 | 0 | G | A | 100.0% | 71.0 / NA | 18 | G129D (GGT→GAT) | SAS1720 | putative membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/7); total (11/7) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTTGAAACTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGGTAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCAATGTCT > BX571857/1868251‑1868430 | gTTGAAACTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAaaga > 2:86798/1‑98 (MQ=255)gTTGAAACTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAaaga > 2:295146/1‑98 (MQ=255) ttGAAACTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAaagaa < 2:307447/98‑1 (MQ=255) tATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCAAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTaa > 2:273178/1‑98 (MQ=255) ttGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGt > 1:74665/1‑98 (MQ=255) ttGGTCCAGCTATCACGCCAACAAGTGCTGAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGt < 2:174135/98‑1 (MQ=255) cATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATg < 2:42062/98‑1 (MQ=255) cgcgCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAAc > 2:214400/1‑98 (MQ=255) aacaaATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAAt < 2:81355/98‑1 (MQ=255) acaaATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATc < 1:229753/98‑1 (MQ=255) tAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATa > 1:233538/1‑98 (MQ=255) tAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATa > 2:320656/1‑98 (MQ=255) tttGTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGa < 1:96298/98‑1 (MQ=255) gTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGc > 1:322032/1‑98 (MQ=255) gTCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGc > 1:322015/1‑98 (MQ=255) tCGTTGCTGATGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCa > 1:203319/1‑98 (MQ=255) aTGTGACGATTAAAAACAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACa < 2:290918/98‑1 (MQ=255) aaaaCAAAGATAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCAATGTCt > 1:221902/1‑98 (MQ=255) | GTTGAAACTATGAAATCAGTTGGTCCATCTATCGCGCCAACAAATGCTAAAGGTACATTTGTCGTTGCTGATGTGACGATTAAAAACAAAGGTAAAGAAGCGTTAACAATTGATAGTTCAATGTTTAAACTGAAATCTGGTGATAAAACATTTGAAGCAGATAATACAGGCTCAATGTCT > BX571857/1868251‑1868430 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |