New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	BX571857	= 2700094	165 (0.930)	22 (0.130)	7/182	15.0	12.6%	coding (2374/2718 nt)	SAS2516	fibrinogen and keratin‑10 binding surface anchored protein
	?	BX571857	2700107 =	149 (0.880)					coding (2361/2718 nt)	SAS2516	fibrinogen and keratin‑10 binding surface anchored protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCCGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  BX571857/2699994‑2700094 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcgctGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGA  >  BX571857/2700107‑2700202                                                                                                                                                                                                       TCCGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGT                                                                                                      >  2:1520211/1‑97  CCGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTC                                                                                                     <  1:1300932/97‑1  CCGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTC                                                                                                     <  1:1606612/97‑1  CCGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTC                                                                                                     <  2:2008980/97‑1  CCGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTC                                                                                                     >  1:2255296/1‑97  CCGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCCGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTC                                                                                                     >  1:219551/1‑97   CGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCG                                                                                                    <  1:2536981/97‑1    GAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGC                                                                                                   <  1:72752/97‑1     AGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCT                                                                                                  <  1:1145814/97‑1     AGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCT                                                                                                  <  2:2580942/97‑1      GTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTG                                                                                                 >  1:1087951/1‑97        CGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGGCTGAGGCGGAGTCGCcatc                                                                                               >  2:2628680/1‑93        CGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATGCTGGGTCGCTGTCTGAGTCTGAGTCGCgatc                                                                                               >  2:2628693/1‑93        CGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGACTCGCTGTC                                                                                               >  2:1906173/1‑97                 AGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGA                                                                                      <  1:1672546/97‑1                    CTGGGTCGCCGTCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATC                                                                                   <  2:1913584/97‑1                      ggGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGGATCGCTATCTGATTCGGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGC                                                                                 <  2:676820/95‑1                        GTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTg                                                                               >  2:2016806/1‑96                         TCGCTGTCTCAATCTGAGTCGCTGTCTGTGTCTGAATCGCTGTCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCCGAATCGCTAT                                                                              <  2:189538/97‑1                            CTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTG                                                                           >  1:1613463/1‑97                                CTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGCCTGAATCGCTATCTGAAgc                                                                       >  2:2430754/1‑95                                 TGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCT                                                                      >  1:2308642/1‑97                                     TCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAATCGCGATCTGAGTCTGAGTCGCTGTCTGCATCTGggg                                                                  >  2:1671124/1‑94                                      CTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATC                                                                 >  1:1366349/1‑97                                             GCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCT                                                          <  2:148727/97‑1                                                          gagTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAATCGC                                             <  2:1583832/94‑1                                                                     TGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAACCTGAGTCGCTGTCTGAG                                                       <  2:1178356/76‑1                                                                     TGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAACCTGAGTCGCTGTCTGAG                                                       >  1:1178356/1‑76                                                                                   TGTCTGAGTCTGTGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGTGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGA                    <  2:2101563/97‑1                                                                                         taTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGA              <  2:2612595/95‑1                                                                                         taTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGA              <  1:300734/95‑1                                                                                            CTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAatc           <  2:578052/97‑4                                                                                             TGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCG          <  2:645723/97‑1                                                                                                GTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTG       <  2:2524371/97‑1                                                                                                  CGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTC     >  1:7674/1‑97                                                                                                  CGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTC     >  2:713906/1‑97                                                                                                  CGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGCCTGAGTCGCTGgc     >  2:660827/1‑95                                                                                                   GCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCT    >  2:2208546/1‑97                                                                                                     TGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGA  >  1:519156/1‑97                                                                                                     TGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGA  >  1:2390586/1‑97                                                                                                     TGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGA  <  2:983574/97‑1                                                                                                     TGTCTGAGTCGGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGA  <  2:2653695/97‑1                                                                                                                                                                                                       TCCGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  BX571857/2699994‑2700094 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcgctGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGA  >  BX571857/2700107‑2700202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.