Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BX571857	1,937,435	A→G	7.6%	noncoding (62/90 nt)	tRNA Ser anticodon TGA_ Cove score 61.04 ←	tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BX571857	1,937,435	0	A	G	7.6%	288.3 / 4.0	80	noncoding (62/90 nt)	tRNA Ser anticodon TGA_ Cove score 61.04	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (42/29);  new base G (3/4);  total (46/34)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGAACCCGCGATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCGAACCTGCG  >  BX571857/1937339‑1937531                                                                                                 |                                                                                                 tCGGACCCGCGCTCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACCCTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGa                                                                                                  <  2:519051/97‑1 (MQ=255) tCGAACCCGCGATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGa                                                                                                  >  2:1156972/1‑97 (MQ=255) tCGAACCCGCGATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGa                                                                                                  >  2:127063/1‑97 (MQ=255) tCGAACCCGCGATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGa                                                                                                  >  2:141232/1‑97 (MQ=255)  cGAACCCGCGATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAg                                                                                                 <  1:860665/97‑1 (MQ=255)    aaCCCGCGATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGcc                                                                                               <  1:746257/97‑1 (MQ=255)      cccGCGATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGt                                                                                             >  2:1097889/1‑97 (MQ=255)      cccGCGATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGt                                                                                             >  1:745514/1‑97 (MQ=255)       ccGCGATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGtt                                                                                            <  2:1051762/97‑1 (MQ=255)       ccGCGATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGtt                                                                                            >  1:659072/1‑97 (MQ=255)        cgcgATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTa                                                                                           <  2:177780/97‑1 (MQ=255)        cgcgATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTa                                                                                           >  1:543160/1‑97 (MQ=255)        cgcgATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTa                                                                                           <  1:559331/97‑1 (MQ=255)          cgATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCCTTAGTAAAACGGAGGAAGCGGGATTCGAACCCCCGCGCGCCGTTCAg                                                                                         >  2:378947/1‑97 (MQ=25)            aTCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGcc                                                                                       <  1:873235/97‑1 (MQ=255)             tctcCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGccc                                                                                      >  1:1481254/1‑97 (MQ=255)              ctcCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGcccc                                                                                     >  2:90316/1‑97 (MQ=255)              ctcCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGcccc                                                                                     >  1:648095/1‑97 (MQ=255)               tcCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCt                                                                                    >  2:455365/1‑97 (MQ=255)                      tGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGtt                                                                             <  2:1047938/97‑1 (MQ=255)                       gACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAGCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGttt                                                                            <  2:1186786/97‑1 (MQ=255)                       gACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGttt                                                                            >  1:207110/1‑97 (MQ=255)                         caggcaggCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTc                                                                          >  1:720923/1‑97 (MQ=255)                         caggcaggCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTc                                                                          >  1:512279/1‑97 (MQ=255)                          aggcaggcGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCa                                                                         >  1:557453/1‑97 (MQ=255)                           ggcaggcGTGCTGACCGCTCCCCCACGAGACCATTAGTAAGGCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCaa                                                                        <  2:1218432/97‑1 (MQ=17)                            gcaggcGTGTTAACCGCTCCACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAg                                                                       <  2:1565663/97‑1 (MQ=255)                            gcaggcGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAg                                                                       <  1:807367/97‑1 (MQ=255)                              aggcGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGAc                                                                     >  1:981377/1‑97 (MQ=255)                               ggcGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGAcc                                                                    <  1:619974/97‑1 (MQ=255)                               ggcGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGAcc                                                                    <  1:245188/97‑1 (MQ=255)                                gcGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCg                                                                   <  2:136362/97‑1 (MQ=255)                                 cGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGa                                                                  <  2:828177/97‑1 (MQ=255)                                 cGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGa                                                                  >  1:422260/1‑97 (MQ=255)                                    gtTAACCGCTACACTGCGAGACCAATAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATcc                                                               <  1:1293443/97‑1 (MQ=255)                                     ttAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATccc                                                              >  1:1323961/1‑97 (MQ=255)                                     ttAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATccc                                                              >  1:455660/1‑97 (MQ=255)                                     ttAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATccc                                                              >  1:96330/1‑97 (MQ=255)                                        aCCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTc                                                           <  1:438094/97‑1 (MQ=255)                                        aCCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTc                                                           <  1:438074/97‑1 (MQ=255)                                         ccGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCa                                                          <  1:921617/97‑1 (MQ=255)                                          cGCTACACTACGAGACCATTAGTAAAGCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAg                                                         <  1:1497195/97‑1 (MQ=255)                                          cGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGGTTTCAAGACCGATCCCTTCAg                                                         >  2:207488/1‑97 (MQ=255)                                              acacTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCgg                                                     >  2:213920/1‑97 (MQ=255)                                                       gaCCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGGCGGTGTTCAAGACCGATCCCCTCAGCCGGACTAgggca                                            >  2:1118577/1‑95 (MQ=14)                                                        aCCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGCCGGTTTTCAAGACCGATCCCTTCCGCCGGCCTTGGGTac                                           >  2:1315679/1‑96 (MQ=14)                                                         ccATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTCTTCAAGACCGATCCCTTCAGCCGGACTTGGGTAtt                                          >  2:1351004/1‑97 (MQ=255)                                                          cATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATtc                                         >  1:1203137/1‑97 (MQ=255)                                                           aTTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATtcc                                        >  2:1409012/1‑97 (MQ=255)                                                            ttAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATtcct                                       >  1:781793/1‑97 (MQ=40)                                                             tAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATtcctc                                      >  2:148685/1‑97 (MQ=39)                                                                tAAAGCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCaa                                   <  1:1283856/97‑1 (MQ=18)                                                                  aaaCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCaaaa                                 >  2:1467355/1‑97 (MQ=38)                                                                   ggcGGAGGAAGAGGGATTCGAACCCCCGCGGGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAAt                                <  2:1287474/95‑1 (MQ=16)                                                                    aCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAAtt                               <  2:1333436/97‑1 (MQ=38)                                                                    aCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAAtt                               >  2:986027/1‑97 (MQ=37)                                                                       gaggaAGAGGGATTCGAACCCCCGCGGCCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATtata                            <  2:477186/97‑1 (MQ=255)                                                                       gaggaAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATtata                            >  2:905748/1‑97 (MQ=40)                                                                        aggaAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTatat                           <  2:113571/97‑1 (MQ=255)                                                                         ggaAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATg                          <  1:611665/97‑1 (MQ=255)                                                                         gcaggagGGATTCGAACCCCCGCGACCCGTTAAGCCCCTGTCGGTTTTCAAGACCGAGCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATg                          <  1:877804/93‑1 (MQ=14)                                                                           aaGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGa                        <  1:137903/97‑1 (MQ=255)                                                                            agagGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGaa                       >  2:746388/1‑96 (MQ=11)                                                                            agagGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGAc                       >  1:801179/1‑97 (MQ=255)                                                                             gagGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGAcc                      <  1:480466/97‑1 (MQ=255)                                                                             gagGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGAcc                      >  1:127987/1‑97 (MQ=255)                                                                             gagGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGAcc                      >  1:918635/1‑97 (MQ=255)                                                                              agGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCt                     >  2:790677/1‑97 (MQ=255)                                                                               gggATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCtt                    >  1:906838/1‑97 (MQ=11)                                                                               gggATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCtt                    >  2:468896/1‑97 (MQ=255)                                                                                 gATTCGAACCCCCGCGGCCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTCGc                  >  2:878150/1‑97 (MQ=255)                                                                                 gATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGc                  >  2:685136/1‑97 (MQ=255)                                                                                    tCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAgg               <  2:1275854/97‑1 (MQ=255)                                                                                     cGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGa              >  2:111033/1‑97 (MQ=255)                                                                                        aCCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTc           >  1:963105/1‑97 (MQ=255)                                                                                         cccccGCGCGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCg          <  2:1293953/97‑1 (MQ=255)                                                                                         cccccGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGGCTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCg          >  2:1290672/1‑97 (MQ=255)                                                                                         cccccGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCg          <  1:69231/97‑1 (MQ=255)                                                                                         cccccGCGAGCCGTTAAGCCCCTGTCGGCTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCg          >  1:1039674/1‑97 (MQ=255)                                                                                          ccccGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCGa         >  2:194338/1‑97 (MQ=255)                                                                                          ccccGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCGa         >  1:364077/1‑97 (MQ=255)                                                                                           cccGCGGGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCGaa        <  2:1498760/97‑1 (MQ=255)                                                                                           cccGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCGaa        <  1:1206863/97‑1 (MQ=255)                                                                                             cgcgAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGCTCCCTTCAGCCGGACTTGGGTATTCCTCCACAATTATATGGACCTTGCAGGACTCGAAcc      >  1:1405542/1‑97 (MQ=255)                                                                                                 aGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCGAACCTGCg  >  1:1246730/1‑97 (MQ=255)                                                                                                 aGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCGAACCTGCg  <  1:960511/97‑1 (MQ=255)                                                                                                 |                                                                                                 TCGAACCCGCGATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCGAACCTGCG  >  BX571857/1937339‑1937531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑