| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BX571857 | 1,937,436 | G→C | 6.6% | noncoding (61/90 nt) | tRNA Ser anticodon TGA_ Cove score 61.04 ← | tRNA‑Ser |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BX571857 | 1,937,436 | 0 | G | C | 6.6% | 314.9 / 6.1 | 81 | noncoding (61/90 nt) | tRNA Ser anticodon TGA_ Cove score 61.04 | tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base G (44/31); new base C (3/3); total (47/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGAACCCGCGATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCGAACCTGCGA > BX571857/1937340‑1937532 | cGAACCCGCGATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAg < 1:860665/97‑1 (MQ=255) aaCCCGCGATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGcc < 1:746257/97‑1 (MQ=255) cccGCGATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGt > 2:1097889/1‑97 (MQ=255) cccGCGATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGt > 1:745514/1‑97 (MQ=255) ccGCGATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGtt < 2:1051762/97‑1 (MQ=255) ccGCGATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGtt > 1:659072/1‑97 (MQ=255) cgcgATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTa > 1:543160/1‑97 (MQ=255) cgcgATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTa < 1:559331/97‑1 (MQ=255) cgcgATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTa < 2:177780/97‑1 (MQ=255) cgATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCCTTAGTAAAACGGAGGAAGCGGGATTCGAACCCCCGCGCGCCGTTCAg > 2:378947/1‑97 (MQ=25) aTCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGcc < 1:873235/97‑1 (MQ=255) tctcCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGccc > 1:1481254/1‑97 (MQ=255) ctcCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGcccc > 2:90316/1‑97 (MQ=255) ctcCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGcccc > 1:648095/1‑97 (MQ=255) tcCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCt > 2:455365/1‑97 (MQ=255) tGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGtt < 2:1047938/97‑1 (MQ=255) gACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAGCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGttt < 2:1186786/97‑1 (MQ=255) gACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGttt > 1:207110/1‑97 (MQ=255) caggcaggCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTc > 1:720923/1‑97 (MQ=255) caggcaggCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTc > 1:512279/1‑97 (MQ=255) aggcaggcGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCa > 1:557453/1‑97 (MQ=255) ggcaggcGTGCTGACCGCTCCCCCACGAGACCATTAGTAAGGCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCaa < 2:1218432/97‑1 (MQ=17) gcaggcGTGTTAACCGCTCCACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAg < 2:1565663/97‑1 (MQ=255) gcaggcGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAg < 1:807367/97‑1 (MQ=255) aggcGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGAc > 1:981377/1‑97 (MQ=255) ggcGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGAcc < 1:619974/97‑1 (MQ=255) ggcGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGAcc < 1:245188/97‑1 (MQ=255) gcGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCg < 2:136362/97‑1 (MQ=255) cGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGa < 2:828177/97‑1 (MQ=255) cGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGa > 1:422260/1‑97 (MQ=255) gtTAACCGCTACACTGCGAGACCAATAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATcc < 1:1293443/97‑1 (MQ=255) ttAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATccc > 1:96330/1‑97 (MQ=255) ttAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATccc > 1:1323961/1‑97 (MQ=255) ttAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATccc > 1:455660/1‑97 (MQ=255) aCCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTc < 1:438074/97‑1 (MQ=255) aCCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTc < 1:438094/97‑1 (MQ=255) ccGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCa < 1:921617/97‑1 (MQ=255) cGCTACACTACGAGACCATTAGTAAAGCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAg < 1:1497195/97‑1 (MQ=255) cGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGGTTTCAAGACCGATCCCTTCAg > 2:207488/1‑97 (MQ=255) acacTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCgg > 2:213920/1‑97 (MQ=255) gaCCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGGCGGTGTTCAAGACCGATCCCCTCAGCCGGACTAgggca > 2:1118577/1‑95 (MQ=14) aCCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGCCGGTTTTCAAGACCGATCCCTTCCGCCGGCCTTGGGTac > 2:1315679/1‑96 (MQ=14) ccATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTCTTCAAGACCGATCCCTTCAGCCGGACTTGGGTAtt > 2:1351004/1‑97 (MQ=255) cATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATtc > 1:1203137/1‑97 (MQ=255) aTTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATtcc > 2:1409012/1‑97 (MQ=255) ttAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATtcct > 1:781793/1‑97 (MQ=40) tAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATtcctc > 2:148685/1‑97 (MQ=39) tAAAGCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCaa < 1:1283856/97‑1 (MQ=18) aaaCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCaaaa > 2:1467355/1‑97 (MQ=38) ggcGGAGGAAGAGGGATTCGAACCCCCGCGGGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAAt < 2:1287474/95‑1 (MQ=16) aCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAAtt > 2:986027/1‑97 (MQ=37) aCGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAAtt < 2:1333436/97‑1 (MQ=38) gaggaAGAGGGATTCGAACCCCCGCGGCCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATtata < 2:477186/97‑1 (MQ=255) gaggaAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATtata > 2:905748/1‑97 (MQ=40) aggaAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTatat < 2:113571/97‑1 (MQ=255) ggaAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATg < 1:611665/97‑1 (MQ=255) gcaggagGGATTCGAACCCCCGCGACCCGTTAAGCCCCTGTCGGTTTTCAAGACCGAGCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATg < 1:877804/93‑1 (MQ=14) aaGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGa < 1:137903/97‑1 (MQ=255) agagGGATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGaa > 2:746388/1‑96 (MQ=11) agagGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGAc > 1:801179/1‑97 (MQ=255) gagGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGAcc > 1:127987/1‑97 (MQ=255) gagGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGAcc > 1:918635/1‑97 (MQ=255) gagGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGAcc < 1:480466/97‑1 (MQ=255) agGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCt > 2:790677/1‑97 (MQ=255) gggATTCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCtt > 1:906838/1‑97 (MQ=11) gggATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCtt > 2:468896/1‑97 (MQ=255) gATTCGAACCCCCGCGGCCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTCGc > 2:878150/1‑97 (MQ=255) gATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGc > 2:685136/1‑97 (MQ=255) tCGAACCCCCGCGGCCCGTTAAGGCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAgg < 2:1275854/97‑1 (MQ=255) cGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGa > 2:111033/1‑97 (MQ=255) aCCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTc > 1:963105/1‑97 (MQ=255) cccccGCGCGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCg < 2:1293953/97‑1 (MQ=255) cccccGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGGCTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCg > 2:1290672/1‑97 (MQ=255) cccccGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCg < 1:69231/97‑1 (MQ=255) cccccGCGAGCCGTTAAGCCCCTGTCGGCTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCg > 1:1039674/1‑97 (MQ=255) ccccGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCGa > 2:194338/1‑97 (MQ=255) ccccGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCGa > 1:364077/1‑97 (MQ=255) cccGCGGGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCGaa < 2:1498760/97‑1 (MQ=255) cccGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCGaa < 1:1206863/97‑1 (MQ=255) cgcgAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGCTCCCTTCAGCCGGACTTGGGTATTCCTCCACAATTATATGGACCTTGCAGGACTCGAAcc > 1:1405542/1‑97 (MQ=255) aGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCGAACCTGCg < 1:960511/97‑1 (MQ=255) aGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCGAACCTGCg > 1:1246730/1‑97 (MQ=255) gCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCGAACCTGCGa > 1:909656/1‑97 (MQ=255) | CGAACCCGCGATCTCCTGCGTGACAGGCAGGCGTGTTAACCGCTACACTACGAGACCATTAGTAAAACGGAGGAAGAGGGATTCGAACCCCCGCGAGCCGTTAAGCCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCGGACTTGGGTATTCCTCCAAAATTATATGGACCTTGCAGGACTCGAACCTGCGA > BX571857/1937340‑1937532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |