| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BX571858 | 20,628:1 | +G | 100% | coding (288/306 nt) | pSAS24 → | putative exported protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BX571858 | 20,628 | 1 | . | G | 100.0% | 51.9 / NA | 13 | K96K (AAA→AAG) | pSAS24 | putative exported protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (5/8); total (5/8) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTATAAATTGGATTTTTTAGGTTATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAA‑ATATTCCTAGCTTTTTAAAATGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑G > BX571858/20532‑20652 | ttATAAATTGGATTTTTTAGGTTATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACaaaa < 2:323406/97‑1 (MQ=255)ttATAAATTGGATTTTTTAGGTTATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACaaaa < 2:323428/97‑1 (MQ=255) aaaTTGGATTTTTTAGGTTATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACaaaagata < 2:153659/97‑5 (MQ=255) tATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAAt > 2:376810/1‑97 (MQ=255) aTGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATg < 1:273505/97‑1 (MQ=255) cTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtga < 2:354609‑M2/97‑6 (MQ=255) gAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagt > 1:181728‑M2/1‑84 (MQ=255) tatGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgat > 2:102622‑M2/1‑81 (MQ=255) aaaTCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaa > 2:295215‑M2/1‑70 (MQ=255) tCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAACGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgttataacttgaaaatg < 1:124456‑M2/97‑31 (MQ=255) tCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatg < 1:364723‑M2/97‑31 (MQ=255) gggAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgt < 2:346342‑M2/97‑38 (MQ=255) atGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaa < 2:8308‑M2/97‑45 (MQ=255) aGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaagggaataaaaaca < 2:335280‑M2/97‑58 (MQ=255) aTACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaataaaaacattt < 1:87050‑M2/97‑61 (MQ=255) cATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaataaaaacattttag > 1:215448‑M2/1‑34 (MQ=255) | TTATAAATTGGATTTTTTAGGTTATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAA‑ATATTCCTAGCTTTTTAAAATGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑G > BX571858/20532‑20652 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |