| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BX571857 | 797,809 | C→T | 31.8% | Q14* (CAA→TAA) | SAS0715 → | putative helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BX571857 | 797,809 | 0 | C | T | 31.8% | 28.3 / 22.1 | 22 | Q14* (CAA→TAA) | SAS0715 | putative helicase |
| Reads supporting (aligned to +/- strand): ref base C (10/5); new base T (5/2); total (15/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
TCAGTACATTCCAAGAAAATTGTATATTGCAGAAATTGTATATCACTGGGTCGAATGGATAATGTAACAAGATATAAAATAACAGAGAGTTCGCAAAGTTCATCACAAGCATATTATCATCTCTCATTTGAATTGTCGGAACAGCAGTCTTATGCCTCAGAACATATTGTTCGAGCCATTAGAAAGAG > BX571857/797716‑797903 | tCAGTACATTCCAAGAAAATTGTATATTGCAGAAATGGTATATCACTGGGTCGAATGGATAATGTAACAAGATATAAAATAACAGAGAGTTCGTaaa < 1:136575/97‑1 (MQ=255) cATTCCAAGAAAATTGTATATTGCAGAAATTGTATATCACTGGGTCGAATGGATAATGTAACAAGATATAAAATAACAGAGAGTTCGTAAAGTtcat > 1:130560/1‑97 (MQ=255) ccAAGAAAATTGTATATTGCAGAAATTGTATATCACTGGGTCGAATGGATAATGTAACAAGATATAAAATAACAGAGAGTTCGTAAAGTTCATcaca > 1:47763/1‑97 (MQ=255) aaGAAAATTGTATATTGCAGAAATTGTATATCACTGGGTCGAATGGATAATGTAACAAGATATAAAATAACAGAGAGTTCGCAAAGTTCATCACAAg < 1:110603/97‑1 (MQ=255) aGAAAATTGTATATTGCAGAAATTGTATATCACTGGGTCGAATGGATAATGTAACAAGATATAAAATAACAGAGAGTTCGCAAAGTTCATCACAAGc > 1:204498/1‑97 (MQ=255) gTATATTGCAGAAATTGTATATCACTGGGTCGAATGGATAATGTAACAAGATATAAAATAACAGAGAGTTCGCAAAGTTCATCACAAGCATATTatc > 2:102744/1‑97 (MQ=255) ttGCAGAAATTGTATATCACTGGGTCGAATGGATAATGTAACAAGATATAAAATAACAGAGAGTTCGTAAAGTTCATCACAAGCATATTATCAtctc > 2:111086/1‑97 (MQ=255) tGTATATCACTGGGTCGAATGGATAATGTAACAAGATATAAAATAACAGAGAGTTCGCAAAGTTCATCACAAGCATATTATCATCTCTCATTTGAAt < 1:305826/97‑1 (MQ=255) aaTGGATAATGTAACAAGATATAAAATAACAGAGAGTTCGCAAAGTTCATCACAAGCATATTATCATCTCGCATTTGAATTGTCGGAACAGCAGTCt < 1:17426/97‑1 (MQ=255) aTGGATAATGTAACAAGATATAAAATAACAGAGAGTTCGTAAAGTTCATCACAAGCATATTATCATCTCTCATTTGAATTGTCGGAACAGCAGTCtt < 1:196472/97‑1 (MQ=255) ggATAATGTAACAAGATATAAAATAACAGAGAGTTCGTAAAGTTCATCACAAGCATATTATCATCTCTCATTTGAATTGTCGGAACAGCAGTCTTAt > 1:75871/1‑97 (MQ=255) ggATAATGTAACAAGATATAAAATAACAGAGAGTTCGTAAAGTTCATCACAAGCATATTATCATCTCTCATTTGAATTGTCGGAACAGCAGTCTTAt > 1:5189/1‑97 (MQ=255) gATAATGTAACAAGATATAAAATAACAGAGAGTTCGCAAAGTTCATCACAAGCATATTATCATCTCTCATTTGAATTGTCGGAACAGCAGTCTTATg < 1:192532/97‑1 (MQ=255) aTAATGTAACAAGATATAAAATAACAGAGAGTTCGCAAAGTTCATCACAAGCATATTATCATCTCTCATTTGAATTGTCGGAACAGCAGTCTTATGc > 2:327062/1‑97 (MQ=255) gTAACAAGATATAAAATAACAGAGAGTTCGCAAAGTTCATCACAAGCATATTATCATCTCTCATTTGAATTGTCGGAACAGCAGTCTTATGCCTCAg > 1:260798/1‑97 (MQ=255) ataAAATAACAGAGAGTTCGCAAAGTTCATCACAAGCATATTATCATCTCTCATTTGAATTGTCGGAACAGCAGTCTTATGCCTCAGAACATATTGt > 2:309234/1‑97 (MQ=255) taAAATAACAGAGAGTTCGCAAAGTTCATCACAAGCATATTATCATCTCTCATTTGAATTGTCGGAACAGCAGTCTTATGCCTCAGAACATATTGtt > 1:340049/1‑97 (MQ=255) taAAATAACAGAGAGTTCGCAAAGTTCATCACAAGCATATTATCATCTCTCATTTGAATTGTCGGAACAGCAGTCTTATGCCTCAGAACATATTGtt > 2:11669/1‑97 (MQ=255) aaaaTAACAGAGAGTTCGCAAAGTTCATCACAAGCATATTATCATCTCTCATTTGAATTGTCGGAACAGCAGTCTTATGCCTCAGAACATATTGTTc < 2:283518/97‑1 (MQ=255) aaCAGAGAGTTCGCAAAGTTCATCACAAGCATATTATCATCTCTCATTTGAATTGTCGGAACAGCAGTCTTATGCCTCAGAACATATTGTTCGAGcc > 2:341641/1‑97 (MQ=255) aCAGAGAGTTCGCAAAGTTCATCACAAGCATATTATCATCTCTCATTTGAATTGTCGGAACAGCAGTCTTATGCCTCAGAACATATTGTTCGAGCCa > 1:279021/1‑97 (MQ=255) cGCAAAGTTCATCACAAGCATATTATCATCTCTCATTTGAATTGTCGGAACAGCAGTCTTATGCCTCAGAACATATTGTTCGAGCCATTAGAAagag > 2:201728/1‑97 (MQ=255) | TCAGTACATTCCAAGAAAATTGTATATTGCAGAAATTGTATATCACTGGGTCGAATGGATAATGTAACAAGATATAAAATAACAGAGAGTTCGCAAAGTTCATCACAAGCATATTATCATCTCTCATTTGAATTGTCGGAACAGCAGTCTTATGCCTCAGAACATATTGTTCGAGCCATTAGAAAGAG > BX571857/797716‑797903 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |