| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BX571858 | 20,628:1 | +G | 100% | coding (288/306 nt) | pSAS24 → | putative exported protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BX571858 | 20,628 | 1 | . | G | 100.0% | 182.3 / NA | 41 | K96K (AAA→AAG) | pSAS24 | putative exported protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (23/18); total (23/18) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGGATTTTTTAGGTTATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAA‑ATATTCCTAGCTTTTTAAAATGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑G > BX571858/20540‑20652 | tgtaTTTTTTAGGTTATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTcc < 2:2051716/94‑1 (MQ=255)tGGATTTTTTAGGTTATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTcc > 2:1282309/1‑97 (MQ=255)tGGATTTTTTAGGTTATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTcc > 1:222285/1‑97 (MQ=255) ggATTTTTTAGGTTATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCt < 1:1030541/97‑1 (MQ=255) ggATTTTTTAGGTTATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTACGATAGAATACATTACAAAAGATATTccc > 1:961309/1‑96 (MQ=255) aTTTTTTAGGTTATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAg > 2:891359/1‑97 (MQ=255) ttttttAGGTTATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGc > 1:648954/1‑97 (MQ=255) ttttAGGTTATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTACTAGCtt > 2:673292/1‑97 (MQ=255) ttttAGGTTATGATCCTACAAAAGAATATGTGAAAGTAAAACATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCtt < 1:1078364/97‑1 (MQ=255) tAGGTTATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCttttt > 1:289567/1‑97 (MQ=255) aTGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATg > 2:1337888/1‑97 (MQ=255) tGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGa > 2:823433/1‑97 (MQ=255) cTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtga > 2:1396700‑M2/1‑92 (MQ=255) cTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtga < 2:2108718‑M2/97‑6 (MQ=255) cTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGCTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtga < 2:1548549‑M2/97‑6 (MQ=255) aaaGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgat > 1:708858‑M2/1‑87 (MQ=255) aaGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgata > 2:1419858‑M2/1‑86 (MQ=255) taaGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgat > 2:1368465‑M2/1‑81 (MQ=255) atGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgata > 1:2060477‑M2/1‑80 (MQ=255) tgtgAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataa < 1:1418374‑M2/97‑19 (MQ=255) tgtgAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataa < 1:1418338‑M2/97‑19 (MQ=255) aaaGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaact < 1:2058790‑M2/97‑23 (MQ=255) aaTCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaa > 2:871740‑M2/1‑69 (MQ=255) aaTCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaa > 2:90984‑M2/1‑69 (MQ=255) tCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatg < 1:1324600‑M2/97‑31 (MQ=255) aTAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtt > 2:1575372‑M2/1‑65 (MQ=255) aaGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgttttt < 1:1125551‑M2/97‑36 (MQ=255) aGGGAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttg < 1:942902‑M2/97‑37 (MQ=255) gggAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgt < 2:931177‑M2/97‑38 (MQ=255) gggAAATATGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgt < 2:178467‑M2/97‑38 (MQ=255) atatGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaa > 2:2123348‑M2/1‑55 (MQ=255) tatGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaa < 2:1545381‑M2/97‑44 (MQ=255) tatGTGCGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaa < 1:2126323‑M2/97‑44 (MQ=255) cGTTCGATAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaa < 1:691300‑M2/97‑50 (MQ=255) aTAGAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaataaaaa > 1:631877‑M2/1‑42 (MQ=255) tAGGATACACTACAAAAGATATTCCTAGCTTATTAAAATGAggtgacctatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaataaaaac > 2:11809‑M2/1‑41 (MQ=255) gAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaataaaaacat > 1:47407‑M2/1‑39 (MQ=255) gAATACATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaataaaaacat > 1:1149369‑M2/1‑39 (MQ=255) aCATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaataaaaacatttta < 2:77857‑M2/97‑63 (MQ=255) cATTACAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaataaaaacattttag > 1:934542‑M2/1‑34 (MQ=255) aCAAAAGATATTCCTAGCTTTTTAAAATGAggtgaccgatagtgataaaacttgaaaatgtttttgtaaaaaaaggtaataaaaacattttagacgg < 2:15408‑M2/97‑68 (MQ=255) | TGGATTTTTTAGGTTATGATCCTACAAAAGAATATGTGAAAGTAAATCATAAAGGGAAATATGTGCGTTCGATAGAATACATTACAAAA‑ATATTCCTAGCTTTTTAAAATGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑G > BX571858/20540‑20652 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |