BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BX571857	848,832	T→G	21.6%	G227G (GGT→GGG)	SAS0756 →	thermonuclease precursor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BX571857	848,832	0	T	G	21.6%	258.3 / 87.1	106	G227G (GGT→GGG)	SAS0756	thermonuclease precursor
Reads supporting (aligned to +/- strand): ref base T (21/62); new base G (4/19); total (25/81)
Fisher's exact test for biased strand distribution p-value = 5.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 106 total aligned reads displayed.

CTAACAATACACATGAACAACTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCAATTCAGAATTAAAAA  >  BX571857/848738‑848926

cTAACAATACACATGAACAACTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCa                                                                                              <  1:864021/97‑1 (MQ=255)

  aaCAATACACATGAACAACTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAGTATTTGGAGCGAAGACAACGCTGATTCAGGTCaat                                                                                            <  1:191885/97‑1 (MQ=255)

  aaCAATACACATGAACAACTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCaat                                                                                            <  1:191905/97‑1 (MQ=255)

   aCAATACACATGAACAACTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCaata                                                                                           >  1:292221/1‑97 (MQ=255)

     aaTACACATGAACAACTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCaataat                                                                                         >  1:405169/1‑97 (MQ=255)

     aaTACACATGAACAACTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCaataat                                                                                         <  1:2013857/97‑1 (MQ=255)

     aaTACACATGAACAACTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGGCaataat                                                                                         <  1:1157818/97‑1 (MQ=255)

      aTACACATGAACAACTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATg                                                                                        >  2:1176932/1‑97 (MQ=255)

      aTACACATGAACAACTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATg                                                                                        >  2:1658724/1‑97 (MQ=255)

       tACACATGAACAACTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGc                                                                                       >  1:455532/1‑97 (MQ=255)

       tACACATGAACAACTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGc                                                                                       >  2:74009/1‑97 (MQ=255)

        acacATGAACAACTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGGCAATAATGCt                                                                                      >  1:644698/1‑97 (MQ=255)

          acaTGAACAACTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCa                                                                                    <  1:1325789/97‑1 (MQ=255)

            aTGAACAACTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGGCAATAATGCTCAtt                                                                                  <  2:1080040/97‑1 (MQ=255)

               aacaacTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTa                                                                               <  2:760263/97‑1 (MQ=255)

               aacaacTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGGCAATAATGCTCATTGTa                                                                               <  1:1663566/97‑1 (MQ=255)

                 caacTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTaaa                                                                             >  1:1281542/1‑97 (MQ=255)

                  aacTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTaaaa                                                                            <  2:35613/97‑1 (MQ=255)

                   acTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAg                                                                           <  2:1207276/97‑1 (MQ=255)

                   acTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAg                                                                           >  1:1125401/1‑97 (MQ=255)

                   acTTATAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAg                                                                           <  2:1288250/97‑1 (MQ=255)

                     ttttAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGGCAATAATGCTCATTGTAAAAgtg                                                                         <  1:191142/97‑1 (MQ=255)

                      tttAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAgtgt                                                                        <  1:230840/97‑1 (MQ=255)

                      tttAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAgtgt                                                                        <  1:1910393/97‑1 (MQ=255)

                      tttAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGGCAATAATGCTCATTGTAAAAgtgt                                                                        <  1:475209/97‑1 (MQ=255)

                       ttAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGGCAATAATGCTCATTGTAAAAGTGTc                                                                       <  1:1721585/97‑1 (MQ=255)

                         aaGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCAc                                                                     <  1:1435560/97‑1 (MQ=255)

                            aaaaaGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCActgc                                                                  >  2:305098/1‑97 (MQ=255)

                            aaaaaGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCActgc                                                                  <  2:309878/97‑1 (MQ=255)

                             aaaaGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCActgct                                                                 <  1:1438241/97‑1 (MQ=255)

                             aaaaGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCActgct                                                                 <  1:1438233/97‑1 (MQ=255)

                             aaaaGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCActgct                                                                 <  1:243045/97‑1 (MQ=255)

                                  tGAAGCACAGGCGAAAGAAGAGAAATTAAATATTTGGGGCGAAGACAGCGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAg                                                            <  2:42218/97‑1 (MQ=255)

                                  tGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAg                                                            <  1:1178473/97‑1 (MQ=255)

                                   gAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGt                                                           <  1:100267/97‑1 (MQ=255)

                                   gAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGt                                                           <  2:51142/97‑1 (MQ=255)

                                    aaGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTg                                                          >  1:279269/1‑97 (MQ=255)

                                     aGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTgg                                                         <  1:1290355/97‑1 (MQ=255)

                                       cacaAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCa                                                       >  1:1826207/1‑97 (MQ=255)

                                        acaAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCAc                                                      <  2:904084/97‑1 (MQ=255)

                                         caAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACt                                                     <  2:1095888/97‑1 (MQ=255)

                                         caAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACt                                                     <  1:1944311/97‑1 (MQ=255)

                                         caAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGGCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACt                                                     <  2:1443884/97‑1 (MQ=255)

                                          aaGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACtt                                                    <  2:359898/97‑1 (MQ=255)

                                           aGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACttt                                                   >  2:24002/1‑97 (MQ=255)

                                            gCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACtttt                                                  <  1:1198604/97‑1 (MQ=255)

                                             cGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTta                                                 <  1:843543/97‑1 (MQ=255)

                                              gAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTtat                                                <  1:1565271/97‑1 (MQ=255)

                                               aaaaaaGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTtata                                               <  1:1948608/97‑1 (MQ=255)

                                               aaaaaaGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTtata                                               <  2:103857/97‑1 (MQ=255)

                                                aaaaaGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATaa                                              >  1:666591/1‑97 (MQ=255)

                                                 aaaaGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAAt                                             >  2:673462/1‑97 (MQ=255)

                                                 aaaaGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAAt                                             <  2:1621705/97‑1 (MQ=255)

                                                 aaaaGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAAt                                             <  2:1118079/97‑1 (MQ=255)

                                                 aaaaGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGGCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAAt                                             <  1:524382/97‑1 (MQ=255)

                                                  aaaGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGGCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAAtt                                            <  2:320293/97‑1 (MQ=255)

                                                   aaGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAAttt                                           >  1:1008956/1‑97 (MQ=255)

                                                    agagAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGGCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAAtttt                                          >  1:1598193/1‑97 (MQ=255)

                                                       gaAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGGCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTgg                                       <  1:1262187/97‑1 (MQ=255)

                                                         aaTTAAATATTTGGAGCGAAGACAACGCTGATTCAGGGCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGAt                                     <  1:186274/97‑1 (MQ=255)

                                                            tAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCAc                                  <  2:1390452/97‑1 (MQ=255)

                                                            tAAATATTTGGAGCGAAGACAACGCTGATTCAGGGCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCAc                                  <  2:1424819/97‑1 (MQ=255)

                                                              aaTATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGa                                <  1:76083/97‑1 (MQ=255)

                                                                 ttttGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGatat                             <  1:1798811/96‑1 (MQ=255)

                                                                  tttGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATg                            <  2:383346/97‑1 (MQ=255)

                                                                  tttGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATg                            <  2:1780802/97‑1 (MQ=255)

                                                                  tttGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATg                            <  1:2088017/97‑1 (MQ=255)

                                                                  tttGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATg                            <  2:1401368/97‑1 (MQ=255)

                                                                  tttGGAGCGAAGACAACGCTGATTCAGGGCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATg                            >  1:2094732/1‑97 (MQ=255)

                                                                    tGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGAt                          >  2:1190826/1‑97 (MQ=255)

                                                                    tGGAGCGAAGACAACGCGGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGAt                          >  2:711879/1‑97 (MQ=255)

                                                                      gAGCGAAGACAACGCTGATTCAGGGCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGAttt                        <  1:307653/97‑1 (MQ=255)

                                                                         cGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATttatt                     <  2:529328/97‑1 (MQ=255)

                                                                            aGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATc                  <  2:2102488/97‑1 (MQ=255)

                                                                            aGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATc                  <  2:2063605/97‑1 (MQ=255)

                                                                            aGACAACGCTGATTCAGGGCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATc                  <  2:1741716/97‑1 (MQ=255)

                                                                             gACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCa                 <  2:1426809/97‑1 (MQ=255)

                                                                             gACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCa                 <  1:844875/97‑1 (MQ=255)

                                                                             gACAACGCTGATTCAGGGCAATAATGCGCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCa                 <  1:134192/97‑1 (MQ=255)

                                                                              aCAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCaa                <  1:1386306/97‑1 (MQ=255)

                                                                              aCAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCaa                <  1:1741599/97‑1 (MQ=255)

                                                                               cAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCAAt               <  1:74009/97‑1 (MQ=255)

                                                                               cAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCAAt               <  2:1154561/97‑1 (MQ=255)

                                                                                aaCGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCAAtt              <  1:390417/97‑1 (MQ=255)

                                                                                aaCGCTGATTCAGGGCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCAAtt              <  1:1622254/97‑1 (MQ=255)

                                                                                 aCGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCAATTc             >  1:409866/1‑97 (MQ=255)

                                                                                  cGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCAATTCa            <  2:1417373/97‑1 (MQ=255)

                                                                                   gCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCAATTCAg           <  1:1041202/97‑1 (MQ=255)

                                                                                   gCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCAATTCAg           <  2:1742980/97‑1 (MQ=255)

                                                                                    cTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCAATTCAGa          <  1:2039583/97‑1 (MQ=255)

                                                                                    cTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCAATTCAGa          <  1:731210/97‑1 (MQ=255)

                                                                                    cTGATTCAGGGCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCAATTCAGa          <  1:500016/97‑1 (MQ=255)

                                                                                       aTTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCAATTCAGAAtt       >  2:1861086/1‑97 (MQ=255)

                                                                                       aTTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCAATTCAGAAtt       >  2:1861079/1‑97 (MQ=255)

                                                                                         tCAGGGCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCAATTCAGAATTaa     <  1:382272/97‑1 (MQ=255)

                                                                                          cAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCAATTCAGAATTaaa    >  1:1882978/1‑97 (MQ=255)

                                                                                          cAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCAATTCAGAATTaaa    <  1:954315/97‑1 (MQ=255)

                                                                                          cAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCAATTCAGAATTaaa    <  1:1814832/97‑1 (MQ=255)

                                                                                           aGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGAACTTTTATAATTTTTGGATCACGTTATGATTTATTATCAATTCAGAATTaaaa   <  2:1836350/97‑1 (MQ=255)

                                                                                            ggTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCAATTCAGAATTaaaaa  <  2:279658/97‑1 (MQ=255)

CTAACAATACACATGAACAACTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAGAGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAATGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCACTTTTATAATTTTTGGATCACGATATGATTTATTATCAATTCAGAATTAAAAA  >  BX571857/848738‑848926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`