Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 605,157 T→C 11.6% D1007D (GAT→GAC SAS0521 → bone sialoprotein‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX571857605,1570TC11.6% 943.9 / 37.2 287D1007D (GAT→GACSAS0521bone sialoprotein‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (126/121);  new base C (33/5);  total (159/128)
Fisher's exact test for biased strand distribution p-value = 1.80e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 299 total aligned reads displayed.

ACTCAGACTCAGATAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACA  >  BX571857/605060‑605254
                                                                                                 |                                                                                                 
   cagactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCa                                                                                                >  1:1276593/1‑98 (MQ=255)
   cagactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCa                                                                                                <  2:190143/98‑1 (MQ=40)
   cagactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCa                                                                                                >  1:2966154/1‑98 (MQ=255)
    agactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGactcagactcag                                                                                               >  2:45639/1‑98 (MQ=255)
     gactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGActcagactcaga                                                                                              >  1:1155974/1‑98 (MQ=255)
     gactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGa                                                                                              <  1:853440/98‑1 (MQ=255)
     gactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGa                                                                                              >  1:199026/1‑98 (MQ=255)
       ctcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACa                                                                                            <  1:3447685/98‑1 (MQ=255)
       ctcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACa                                                                                            >  2:548659/1‑98 (MQ=37)
       ctcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACa                                                                                            >  2:3228763/1‑98 (MQ=255)
        tcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGAATCGGACAGCGATTCAGACTCAGATAGCGACGCAGATTCCGACAg                                                                                           >  1:2928414/1‑98 (MQ=255)
         cagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGc                                                                                          >  1:786409/1‑98 (MQ=255)
         cagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGc                                                                                          >  2:395960/1‑98 (MQ=255)
            aTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                                                                       >  1:2061981/1‑98 (MQ=255)
             tAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAtc                                                                                      >  2:3121319/1‑97 (MQ=255)
              aGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTc                                                                                     >  2:3638701/1‑98 (MQ=37)
                   ttcagattcagaTAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACt                                                                                >  1:2540355/1‑98 (MQ=255)
                   ttcagattcagaCAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACt                                                                                <  2:1508707/98‑1 (MQ=25)
                    tcagattcagatGGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                                                               <  2:2935353/98‑1 (MQ=255)
                    tcagattcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTc                                                                               >  2:895944/1‑98 (MQ=18)
                    tcagattcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAGTCAGACTc                                                                               >  2:615481/1‑98 (MQ=255)
                    tcagattcagatAGCGACTCAGACTCAGATAGCGACTCAGACTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGAcgc                                                                               >  2:768436/1‑96 (MQ=11)
                       gattcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGa                                                                            <  2:2489262/98‑1 (MQ=255)
                        attcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGAt                                                                           >  2:2813732/1‑98 (MQ=21)
                         ttcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATa                                                                          <  1:738826/98‑1 (MQ=255)
                         ttcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATa                                                                          >  2:1232471/1‑98 (MQ=255)
                          tcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                                                         >  2:3612963/1‑98 (MQ=255)
                          tcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                                                         <  1:1475659/98‑1 (MQ=255)
                          tcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                                                         <  1:883703/98‑1 (MQ=255)
                                aGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTc                                                                   >  1:1686889/1‑98 (MQ=255)
                                aGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTc                                                                   >  2:1771124/1‑98 (MQ=255)
                                  cGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAg                                                                 <  1:1940496/98‑1 (MQ=255)
                                    actcagactcagATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAt                                                               >  1:3552296/1‑98 (MQ=255)
                                    actcagactcagATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGac                                                               >  2:1141956/1‑97 (MQ=255)
                                     ctcagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAtt                                                              >  1:185718/1‑98 (MQ=255)
                                     ctcagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAtt                                                              >  1:1740227/1‑98 (MQ=255)
                                     ctcagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGact                                                              >  2:869430/1‑96 (MQ=255)
                                     ctcagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAtt                                                              >  2:3465459/1‑98 (MQ=255)
                                      tcagactcagacAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTc                                                             <  2:599380/98‑1 (MQ=255)
                                      tcagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTc                                                             >  2:3186217/1‑98 (MQ=255)
                                      tcagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTc                                                             >  2:659353/1‑98 (MQ=255)
                                         gactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGActcagactcaga                                                          >  2:1296386/1‑98 (MQ=255)
                                          actcagaTAGCGACTCAGATTNGGACAGCGATNCAGNCTCAGATAGCGACTCAGACTCAGACAGCGATTCAGANTCAGATANCGACTCAGATACAGaa                                                         >  2:1862553/1‑97 (MQ=14)
                                          actcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGAc                                                         >  1:2343683/1‑98 (MQ=255)
                                           ctcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACa                                                        <  1:1782204/98‑1 (MQ=255)
                                           ctcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACa                                                        >  1:300940/1‑98 (MQ=255)
                                           ctcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACa                                                        >  1:288125/1‑98 (MQ=255)
                                            tcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAg                                                       >  1:3157172/1‑98 (MQ=255)
                                             cagcTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGt                                                      <  2:1808407/98‑1 (MQ=255)
                                              ggaTAGCGACACAGACTCGGAGAGCGAGTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATGCAGACAGTg                                                     <  2:3416146/97‑1 (MQ=14)
                                                aTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgac                                                   >  1:959424/1‑98 (MQ=255)
                                                      aCTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcagac                                             <  1:1427120/98‑1 (MQ=255)
                                                       cTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagact                                            >  2:1604719/1‑98 (MQ=255)
                                                       cTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACACAGATTCAGACAGTgactcaggcc                                            >  1:2842040/1‑95 (MQ=255)
                                                        tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcagactc                                           <  1:3288332/98‑1 (MQ=255)
                                                        tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagactc                                           >  2:697360/1‑98 (MQ=255)
                                                         cAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcagactca                                          <  1:104100/98‑1 (MQ=255)
                                                         cAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCgactcagactca                                          >  1:348377/1‑98 (MQ=18)
                                                           gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGActcagactcaga                                        >  2:3317104/1‑98 (MQ=255)
                                                            aatCGGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGAt                                       <  2:2401962/96‑1 (MQ=255)
                                                            aTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGAt                                       >  1:4778/1‑98 (MQ=255)
                                                            aTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGActcagactcagac                                       >  2:1042978/1‑97 (MQ=255)
                                                            aTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGAt                                       >  2:1557038/1‑98 (MQ=255)
                                                             ttCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATa                                      >  1:394402/1‑98 (MQ=255)
                                                              tCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGATAg                                     >  2:2633913/1‑98 (MQ=255)
                                                                  aCAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgac                                 >  1:2661010/1‑98 (MQ=255)
                                                                   cAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgact                                >  1:3040331/1‑98 (MQ=255)
                                                                    aGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactc                               >  1:2153208/1‑98 (MQ=255)
                                                                     gCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactca                              >  1:2507996/1‑98 (MQ=255)
                                                                      cGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcag                             <  1:574438/98‑1 (MQ=255)
                                                                       gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcaga                            <  1:1583150/98‑1 (MQ=255)
                                                                       gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcaga                            <  1:531097/98‑1 (MQ=255)
                                                                         ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcagact                          >  1:3524465/1‑98 (MQ=255)
                                                                         ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcagact                          >  1:2295224/1‑98 (MQ=255)
                                                                         ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcagact                          >  1:3370928/1‑98 (MQ=255)
                                                                         ctCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGATAGCgactcagact                          >  2:1667852/2‑98 (MQ=255)
                                                                          gcaaaCTCAGAAAGCGATTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcagactc                         <  2:2160668/94‑1 (MQ=14)
                                                                           cAGCCTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcagactca                        <  2:492488/98‑1 (MQ=255)
                                                                            aGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGactcagactcag                       >  1:2589876/1‑98 (MQ=255)
                                                                            aGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGactcagactcag                       <  1:2368041/98‑1 (MQ=255)
                                                                             gACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGAGACTCAGACTCAGATAGCGActcagactcaga                      >  2:2563959/1‑98 (MQ=11)
                                                                             gACACAGATAGCGGCTCAGATTCAGGCAGCGATTGAGACTCAGATGGCGACTCAGATTCAGGCAGTGACTCAGACTCAGATAGCGActcagactcaga                      <  2:349249/98‑1 (MQ=14)
                                                                               cTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACa                    >  2:2140949/1‑98 (MQ=255)
                                                                               cTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACa                    <  1:806614/98‑1 (MQ=255)
                                                                                tCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAg                   <  2:358792/98‑1 (MQ=255)
                                                                                tCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGATAGCGAATCAGACTCAGACaa                   >  2:1100201/1‑97 (MQ=255)
                                                                                    aTAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgac               >  2:1421229/1‑98 (MQ=255)
                                                                                    aTAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgac               >  2:1382382/1‑98 (MQ=255)
                                                                                     tAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgact              >  1:1672178/1‑98 (MQ=255)
                                                                                        cGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcag           >  2:3001042/1‑98 (MQ=255)
                                                                                        cGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcag           >  1:685797/1‑98 (MQ=255)
                                                                                        cGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcag           <  1:2188274/98‑1 (MQ=255)
                                                                                        cGACTCAGATTCAGACAGCGATGCAGCCGCGGACAGCGACTCAGATTCAGACAGCGACTCAGACGCAGATCGCGACTCAGACTCAGACAGTgactcag           <  2:1717639/98‑1 (MQ=11)
                                                                                        cGACTCAGATTCAGACAGCGATACAGACTCAGATAGCGACTCAGATCCAGACAGTGACTCAGACTCAGAAAGCGACTCAGACTCAGACAGTgactcag           <  2:3335993/98‑1 (MQ=14)
                                                                                         gACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcaga          <  1:3636723/98‑1 (MQ=255)
                                                                                         gACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcaga          <  2:2037476/98‑1 (MQ=255)
                                                                                          attcagactcagacAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACACCGACGCAGACTCAGACAGCgactcagac         >  2:2271352/3‑98 (MQ=11)
                                                                                            tCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagactc       <  2:2507562/98‑1 (MQ=255)
                                                                                              aGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGactcagactcag     >  1:1014166/1‑98 (MQ=255)
                                                                                                aTTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACtcagactcagac   <  2:197663/98‑1 (MQ=255)
                                                                                                 |                                                                                                 
ACTCAGACTCAGATAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACA  >  BX571857/605060‑605254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: