Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 605,193 T→C 6.0% D1019D (GAT→GAC SAS0521 → bone sialoprotein‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX571857605,1930TC 6.0% 1010.0 / 14.0 279D1019D (GAT→GACSAS0521bone sialoprotein‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (126/129);  new base C (18/3);  total (146/133)
Fisher's exact test for biased strand distribution p-value = 1.22e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 303 total aligned reads displayed.

ACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAGCGACTCAGACTCAGATA  >  BX571857/605096‑605290
                                                                                                 |                                                                                                 
actcagactcagATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAt                                                                                                   <  2:1763182/98‑1 (MQ=25)
 ctcagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAtt                                                                                                  >  1:1740227/1‑98 (MQ=255)
  tcagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTc                                                                                                 >  2:3186217/1‑98 (MQ=255)
    agactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAg                                                                                               >  1:1890996/1‑98 (MQ=255)
     gactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGa                                                                                              >  2:2552194/1‑98 (MQ=255)
      actcagaTAGCGACTCAGATTNGGACAGCGATNCAGNCTCAGATAGCGACTCAGACTCAGACAGCGATTCAGANTCAGATANCGACTCAGATACAGaa                                                                                             >  2:1862553/1‑97 (MQ=14)
      actcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGAc                                                                                             >  1:2343683/1‑98 (MQ=255)
       ctcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACa                                                                                            >  1:288060/1‑98 (MQ=255)
        tcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAg                                                                                           >  1:3157172/1‑98 (MQ=255)
         cagcTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGt                                                                                          <  2:1808407/98‑1 (MQ=255)
          agaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTg                                                                                         <  1:3230360/98‑1 (MQ=255)
            aTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgac                                                                                       >  1:959424/1‑98 (MQ=255)
             caGCGATTCAGACTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgact                                                                                      <  2:3450417/97‑1 (MQ=255)
              aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactc                                                                                     >  1:919938/1‑98 (MQ=255)
                 gACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcaga                                                                                  >  1:783992/1‑98 (MQ=255)
                    tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagactc                                                                               >  2:697360/1‑98 (MQ=255)
                     cAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcagactca                                                                              >  1:710822/1‑98 (MQ=255)
                        aTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGAt                                                                           >  1:1073799/1‑98 (MQ=255)
                        aTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGActcagactcagac                                                                           >  2:1042978/1‑97 (MQ=255)
                         ttCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATa                                                                          >  1:394402/1‑98 (MQ=255)
                               cAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgact                                                                    >  1:1586548/1‑98 (MQ=255)
                                aGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactc                                                                   >  1:2153208/1‑98 (MQ=255)
                                  cGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcag                                                                 >  1:2400724/1‑98 (MQ=255)
                                   gATTCAGACTCAGATAGCGGCTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcaga                                                                <  1:1458783/98‑1 (MQ=255)
                                   gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcaga                                                                <  1:828572/98‑1 (MQ=255)
                                   gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcaga                                                                <  1:531097/98‑1 (MQ=255)
                                    aTTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcagac                                                               <  2:1395906/98‑1 (MQ=255)
                                      tCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcagactc                                                             >  1:3492948/1‑98 (MQ=255)
                                        aGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGactcagactcag                                                           >  1:2589876/1‑98 (MQ=255)
                                         gACACAGATAGCGGCTCAGATTCAGGCAGCGATTGAGACTCAGATGGCGACTCAGATTCAGGCAGTGACTCAGACTCAGATAGCGActcagactcaga                                                          <  2:349249/98‑1 (MQ=14)
                                           cTCAGATAGTGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACa                                                        <  2:1137429/98‑1 (MQ=255)
                                           cTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACa                                                        >  1:254326/1‑98 (MQ=255)
                                             cAGATAGCGACTCAGAGTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGt                                                      <  2:2216604/98‑1 (MQ=255)
                                              aGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                                                     <  2:1631697/98‑1 (MQ=255)
                                                aTAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgac                                                   >  2:502695/1‑98 (MQ=255)
                                                aTAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgac                                                   >  1:1680558/1‑98 (MQ=255)
                                                 tAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgact                                                  <  1:3034783/98‑1 (MQ=255)
                                                 gaGCGACTCAGCTTCAGACAGCGATTCAGTCTCAGATAGCGACTCAGATTCAGACAGTGACGCAGACTCAGATAGCGACTCAGACTCAGACAGTgact                                                  <  1:1992720/97‑1 (MQ=14)
                                                    cGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcag                                               >  2:3001042/1‑98 (MQ=255)
                                                    cGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcag                                               <  1:2188274/98‑1 (MQ=255)
                                                    cGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcag                                               <  2:427985/98‑1 (MQ=255)
                                                    cGACTCAGATTCAGACAGCGATGCAGCCGCGGACAGCGACTCAGATTCAGACAGCGACTCAGACGCAGATCGCGACTCAGACTCAGACAGTgactcag                                               <  2:1717639/98‑1 (MQ=11)
                                                    cGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcag                                               <  2:128200/98‑1 (MQ=255)
                                                     gACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcaga                                              <  2:2037476/98‑1 (MQ=255)
                                                      attcagactcagacAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACACCGACGCAGACTCAGACAGCgactcagac                                             >  2:2271352/3‑98 (MQ=11)
                                                       cTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagact                                            >  2:1461382/1‑98 (MQ=255)
                                                          aGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGactcagactcag                                         >  1:1014166/1‑98 (MQ=255)
                                                            actCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGactcagactcag                                                           <  1:3534406/76‑1 (MQ=14)
                                                            actCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGactcagactcag                                                           >  2:3534406/3‑78 (MQ=14)
                                                            aTTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACtcagactcagac                                       >  2:3322269/1‑98 (MQ=255)
                                                            aTTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACtcagactcagac                                       <  2:197663/98‑1 (MQ=255)
                                                             ttCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACCCAGACTCAGACa                                      >  1:1832500/1‑98 (MQ=255)
                                                              tCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAg                                     <  1:1039840/98‑1 (MQ=255)
                                                                aGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCg                                   >  2:2803844/1‑98 (MQ=255)
                                                                aGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCg                                   <  1:1491288/98‑1 (MQ=255)
                                                                  aCAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCgat                                 >  2:471395/1‑98 (MQ=255)
                                                                  aCAGCGATCCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCgat                                 >  1:868087/1‑98 (MQ=255)
                                                                   cAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCgatt                                >  2:1565131/1‑98 (MQ=255)
                                                                   cAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCgatt                                >  1:1226286/1‑98 (MQ=255)
                                                                    aGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCgattc                               >  2:258533/1‑98 (MQ=255)
                                                                    aGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCgattc                               <  1:267788/98‑1 (MQ=255)
                                                                    aGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCgattc                               >  2:2625960/1‑98 (MQ=255)
                                                                     gCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagac                                             >  2:39002/1‑83 (MQ=255)
                                                                     gCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCgattca                              <  1:465831/98‑1 (MQ=255)
                                                                      cGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCgattcag                             >  2:2503593/1‑98 (MQ=255)
                                                                      cGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCgattcag                             <  1:2254509/98‑1 (MQ=255)
                                                                      cGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCgattcag                             >  1:2475025/1‑98 (MQ=255)
                                                                      cGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCgattcag                             <  2:1512490/98‑1 (MQ=255)
                                                                      cGATGCAGACACAGAGAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGCCTCAGACAGTGACTCAGACTCAGACAGCgattcag                             <  2:2936326/98‑1 (MQ=17)
                                                                       gATTCAGCCTCAGATAGCGCCTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCgattcaga                            <  2:3622809/98‑1 (MQ=14)
                                                                       gATGCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCgattcaga                            <  2:3378205/98‑1 (MQ=255)
                                                                        aTTCAGGCTCAGATAGCGACGCAGAGTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCgattcagat                           <  2:2595061/98‑1 (MQ=255)
                                                                        aTTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCgattcagat                           <  1:643668/98‑1 (MQ=255)
                                                                        aTTCAGACTCAGAAAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGactcagactcag                                                           >  2:100722/1‑66 (MQ=11)
                                                                         ttCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCgattcagatt                          >  1:3420618/1‑98 (MQ=255)
                                                                          tCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCgattcagattc                         >  1:573655/1‑98 (MQ=255)
                                                                            agccTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGattcagattcag                       <  2:2447174/95‑1 (MQ=255)
                                                                             gACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGAttcagattcaga                      >  1:2625635/1‑98 (MQ=255)
                                                                               cTCAGATAGCGACGCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATa                    <  2:2157268/98‑1 (MQ=255)
                                                                                tCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAg                   <  2:1530778/98‑1 (MQ=255)
                                                                                tCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAg                   <  1:1771124/98‑1 (MQ=255)
                                                                                 cAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGACTCAGATAGc                  >  2:1912157/1‑98 (MQ=255)
                                                                                  aGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAGCg                 <  1:1946565/98‑1 (MQ=255)
                                                                                   gATAGCGACTCAGAATCAGACAGTGAATCAGACTCAGATAGCGACTCAGACTCCGCGAGGGACTCAGACTCAGAGAGCGATTCCGATTCGGATAgtgc                >  2:3080647/1‑95 (MQ=14)
                                                                                    aTAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAGCgac               >  1:3595962/1‑98 (MQ=255)
                                                                                     tAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAGCgact              <  1:62665/98‑1 (MQ=255)
                                                                                     gggCGACGCAGACTCAGACAGTGACTCAGATTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAGCgact              <  2:3588520/96‑1 (MQ=17)
                                                                                      aGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAGCgactc             >  1:2861831/1‑98 (MQ=255)
                                                                                      aGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAGCgactc             >  1:2915778/1‑98 (MQ=255)
                                                                                       gCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAGCgactca            <  1:375983/98‑1 (MQ=255)
                                                                                        cGACTCAGATTCAGCCCGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAGCgactcag           <  2:2356332/98‑1 (MQ=255)
                                                                                         gACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAGCgactcaga          <  2:673885/98‑1 (MQ=255)
                                                                                           cTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAGCgactcagact        <  1:2711930/98‑1 (MQ=255)
                                                                                           cTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAGCgactcagact        <  2:882542/98‑1 (MQ=255)
                                                                                           cTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAGCgactcagact        >  2:598880/1‑98 (MQ=255)
                                                                                            tCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAGCgactcagactc       >  2:9145/1‑98 (MQ=255)
                                                                                              aGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAGCGactcagactcag     <  2:1312151/98‑1 (MQ=255)
                                                                                              aGATTCAGACAGTGACACAGACTCAGAAAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAGCGactcagactcag     <  1:3322899/98‑1 (MQ=255)
                                                                                               gATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAGCGActcagactcaga    <  1:2968707/98‑1 (MQ=255)
                                                                                                 ttCACACAGTGACTCAGACTCAGATAGCGACCCACACACAGACAGTGACTCAGACTCGGACAGCGAATCGGAGTCAGATAGCGACAAAGACTCAGATa  >  2:2693209/1‑98 (MQ=17)
                                                                                                 |                                                                                                 
ACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAGCGACTCAGACTCAGATA  >  BX571857/605096‑605290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: