Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 2,700,227 A→G 5.7% D747D (GAT→GAC SAS2516 ← fibrinogen and keratin‑10 binding surface anchored protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX5718572,700,2270AG 5.7% 614.8 / 2.3 171D747D (GAT→GACSAS2516fibrinogen and keratin‑10 binding surface anchored protein
Reads supporting (aligned to +/- strand):  ref base A (64/94);  new base G (10/2);  total (74/97)
Fisher's exact test for biased strand distribution p-value = 5.29e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 200 total aligned reads displayed.

TCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTG  >  BX571857/2700132‑2700323
                                                                                               |                                                                                                
tctgaatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTcgctgtctgagtctgaatcgctgtctgagtctgaat                                                                                                 >  2:2298262/1‑97 (MQ=255)
tctgaatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATctgaatctgaat                                                                                                 >  2:85617/1‑97 (MQ=255)
 ctgaatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCtgaatctgaatc                                                                                                <  1:2330397/97‑1 (MQ=255)
  tgaatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCg                                                                                               >  1:1798450/1‑97 (MQ=255)
  tgaatcGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCACTGTCTGAGTCTGAATCGCTGTCTGAATCTGAGTCg                                                                                               >  2:483704/1‑97 (MQ=255)
    aatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCt                                                                                             <  2:547519/97‑1 (MQ=255)
    aatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCt                                                                                             <  1:2436403/97‑1 (MQ=255)
    aatcGCTGTCTGAATCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCt                                                                                             <  2:2027944/97‑1 (MQ=255)
     gtcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTg                                                                                            <  2:1700473/96‑1 (MQ=255)
     atcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTg                                                                                            <  2:1301344/97‑1 (MQ=255)
     atcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTg                                                                                            <  1:1816556/97‑1 (MQ=255)
     atcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTg                                                                                            <  1:1595791/97‑1 (MQ=255)
      tcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTctgagtctgaatcgctgtctgagtctgaatcgctgt                                                                                           >  1:1220717/1‑97 (MQ=255)
        gCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGGGTCTGAATCGCTGTCTGAATCGGAATCGCTgtct                                                                                         >  2:820482/1‑97 (MQ=255)
        gCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAGTCGCTgtct                                                                                         >  2:274904/1‑97 (MQ=255)
        gCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCGGAATCGCTGGCTGAAGCTGAGTCGCTggct                                                                                         >  2:1077747/1‑97 (MQ=14)
          tGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtctga                                                                                       <  2:1624252/97‑1 (MQ=255)
          tGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGAAGTCTGAATCTGAATCGCTgtctga                                                                                       >  2:119783/1‑97 (MQ=255)
           gtctgagtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTgtctgag                                                                                      >  2:2155082/1‑97 (MQ=11)
           gtctgagtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTgtctgag                                                                                      <  1:2155082/97‑1 (MQ=11)
             ctgagtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtctgagtc                                                                                    <  1:279578/97‑1 (MQ=255)
                cgtccgaATCGCTATCCGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtctgagtctga                                                                                 <  2:1512579/96‑1 (MQ=255)
                agtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtctgagtctga                                                                                 <  1:1876360/97‑1 (MQ=255)
                agtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtctgagtctga                                                                                 <  2:1150561/97‑1 (MQ=255)
                agtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCCGAATCGCTatctgaacctga                                                                                 >  2:1733382/1‑97 (MQ=17)
                 gtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGGGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGaa                                                                                <  1:2138443/97‑1 (MQ=255)
                 gtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAatcgctgtctgagtctgaatcgctgtctgagtctgag                                                                                >  1:2209772/1‑96 (MQ=255)
                 gtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGACTCTGAATCGCTGTCTGAGTCTGaa                                                                                <  2:1489860/97‑1 (MQ=255)
                    tgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCCGAATCGCTatctgaacct                                                                                   <  1:10817/91‑5 (MQ=17)
                       aTCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGATTCGCTa                                                                          <  1:1033630/97‑1 (MQ=255)
                        tCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAt                                                                         >  1:1655842/1‑97 (MQ=255)
                        tCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAt                                                                         >  1:1496077/1‑97 (MQ=255)
                         cGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCGGAATCGcggtc                                                                        >  2:1089953/1‑93 (MQ=17)
                          gCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtct                                                                       >  1:2180091/1‑97 (MQ=255)
                            tttCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctga                                                                     <  2:756407/95‑1 (MQ=255)
                            tATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctga                                                                     <  1:482542/97‑1 (MQ=255)
                            tATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctga                                                                     <  1:841696/97‑1 (MQ=255)
                            caTCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctga                                                                     <  2:1709602/96‑1 (MQ=255)
                             aTCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctgag                                                                    <  1:1868194/97‑1 (MQ=255)
                             aTCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctgag                                                                    <  1:1967063/97‑1 (MQ=255)
                             aTCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctgag                                                                    >  1:1023056/1‑97 (MQ=255)
                             aTCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctgag                                                                    <  1:631874/97‑1 (MQ=255)
                             aTCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAACCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctgag                                                                    >  2:134967/1‑97 (MQ=14)
                               cTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGCATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAGTCGCTAtctgagtc                                                                  >  2:1874341/1‑97 (MQ=255)
                               cTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTAtctgagcc                                                                  >  2:1037383/1‑95 (MQ=255)
                                tGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGACTCGCTGtctgagtgt                                                                 >  2:947009/1‑95 (MQ=17)
                                tGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctgagtct                                                                 >  2:1200354/1‑97 (MQ=255)
                                tGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctgagtct                                                                 >  1:498301/1‑97 (MQ=255)
                                 gATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctgagtctg                                                                <  1:1733742/97‑1 (MQ=255)
                                 gATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCCGAATCGCTGTCTGAATCTGAATCACTGtctgaatctg                                                                <  1:1153751/97‑1 (MQ=11)
                                  aTTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctgagtctga                                                               >  1:1326552/1‑97 (MQ=255)
                                    tctgagtcgctgtctgagtctgcgtcgctgtctgagTCTGAATCGCTGTCTGAACCTGAATCGCTGTCTGAGTCTGAATCGCTATctgagtctgagt                                                             >  1:1771365/1‑97 (MQ=255)
                                     ctgagtcgctgtctgagtctgagtcgctgtctgagtCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCtgagtctgagtc                                                            <  1:1718594/97‑1 (MQ=255)
                                     ctgagtcgctgtctgagtctgagtcgctgtctgagtCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCtgagtctgagtc                                                            >  2:1598027/1‑97 (MQ=255)
                                     ctgagtcgctgtctgagtctgagtcgctgtctgagtCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCtgagtctgagtc                                                            >  1:1288941/1‑97 (MQ=255)
                                     ctgagtcgctgtctgagtctgagtcgctgtctgagtCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCtgagtctgagtc                                                            <  1:1693500/97‑1 (MQ=255)
                                       gagtcgctgtctgagtctgagtcgctgtctgagtctGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCAc                                                          <  2:1964901/97‑1 (MQ=255)
                                       gagtcgctgtctgagtctgagtcgctgtctgagtctGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCAc                                                          <  2:1181360/97‑1 (MQ=255)
                                       gagtcgctgtctgagtctgagtcgctgtctgagtctGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCAc                                                          <  1:1979545/97‑1 (MQ=255)
                                                 ctgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAGTCTGAATCGCTGTCTGAGCCTGAGTCGCTCTCTGACTCTGAGTCACTAGCTGAGTc                                                >  2:242761/1‑97 (MQ=11)
                                                 ctgagtctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGCATCGCTATCTGAGTCTGAGTCACTGCCTGCATc                                                >  2:359055/1‑97 (MQ=255)
                                                   gagtctgtgtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTg                                              <  2:1849530/97‑1 (MQ=255)
                                                      tctgagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACt                                           <  2:1217002/97‑1 (MQ=255)
                                                         gagtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCAc                                        <  2:1776619/97‑1 (MQ=255)
                                                           gtcgctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTa                                      >  1:64447/1‑97 (MQ=255)
                                                               ctgtctgagtctgaGTCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctg                                  <  2:166364/97‑1 (MQ=255)
                                                               ctgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctg                                  <  1:768869/97‑1 (MQ=255)
                                                                tgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctga                                 <  1:69213/97‑1 (MQ=255)
                                                                tgtctgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctga                                 <  1:58114/97‑1 (MQ=255)
                                                                 gcctgcgtctgaATCGCCGCCTCAATCTGAATCGCTGTCCGCGTCTGAATCGCTATCTGACTCCGCGTCCCCGTCCGCCTCCGACTCACCatctgcc                                >  2:600083/3‑95 (MQ=2)
                                                                   ctgagtctgaATCGCTGTCTGAATCTGAATCGCTgtctg                                                                                        <  2:2189527/39‑1 (MQ=255)
                                                                    tgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctgaatct                             >  1:2042217/1‑97 (MQ=255)
                                                                    tgagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctgaatcc                             >  2:1915836/1‑96 (MQ=255)
                                                                     gagtctgaGTCGCTGTCTGAATCTGATTCGCTGTCGGTGTCTGAGTCTCTATCGCAGTCTGAGTCACTGTCTGAATCTGACTCACTatctgaatctg                            <  1:1278171/97‑1 (MQ=11)
                                                                     gagtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctgaatctg                            <  1:1395074/97‑1 (MQ=255)
                                                                      agtctgaGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctgaatctga                           <  2:1790010/97‑1 (MQ=37)
                                                                       gtctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAg                          <  1:2060135/97‑1 (MQ=255)
                                                                        tctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGt                         <  1:451526/97‑1 (MQ=255)
                                                                         ctgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTc                        >  2:1892617/1‑97 (MQ=255)
                                                                          tgaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCg                       >  1:1312344/1‑97 (MQ=255)
                                                                           gaATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGc                      <  2:1398127/97‑1 (MQ=255)
                                                                            aaTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCt                     >  1:2274161/1‑97 (MQ=255)
                                                                             aTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGACTCGCTATCTGAATCTGAGTCGCTa                    >  1:1945119/1‑97 (MQ=255)
                                                                             aTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGCCTGAGTCACTGTCTGAATCTGACTCACGATCTGAATCTGAGTCGcga                    >  1:2151064/1‑95 (MQ=255)
                                                                              tCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTAt                   <  2:2147104/97‑1 (MQ=255)
                                                                                gCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTAtct                 >  1:8081/1‑97 (MQ=39)
                                                                                 cTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTAtctg                <  2:2140535/97‑1 (MQ=37)
                                                                                  tGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGACTCACTATCTGAATCCGAGTCGCTAtctga               >  2:446362/1‑97 (MQ=255)
                                                                                  tGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTAtctga               >  1:1193392/1‑97 (MQ=39)
                                                                                   gTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTAtctgag              >  2:382741/1‑97 (MQ=255)
                                                                                      tgaatctgaatcGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGCCTCACTATCTGAATCTGAGTCGCTAtctgagtct           >  2:864308/1‑97 (MQ=255)
                                                                                      tgaatctgaatcGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTAtctgagtct           <  1:2020945/97‑1 (MQ=255)
                                                                                        gatctgagtcGCTGTCTGAGTCTGAGTCGCTATCTGAGGCTGAGGCGCTGTCTGAATCTGACTCACTGTCTGAATCTGAGTCGCTAtctgagtctga         <  2:263547/96‑1 (MQ=11)
                                                                                        attctgagtcGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTAtctgagtctga         <  2:679448/95‑1 (MQ=255)
                                                                                        aatctgaatcGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTAtctgagtctga         <  1:1635980/97‑1 (MQ=255)
                                                                                         atctgaatcGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTatctgaa                                >  1:2097822/1‑73 (MQ=35)
                                                                                              aatcGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGACTCGCTATCTGAATCGGAGGCGCTATCTGAGTCTGAAtcgct   >  2:629089/1‑97 (MQ=11)
                                                                                              aatcGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcgct   <  2:2209095/97‑1 (MQ=255)
                                                                                               atcGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTAtctgag              >  1:410492/1‑85 (MQ=37)
                                                                                               atcGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGtcgcta  >  1:1301058/1‑96 (MQ=255)
                                                                                               |                                                                                                
TCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCACTGTCTGAATCTGACTCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTG  >  BX571857/2700132‑2700323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: