Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 431,505 T→C 7.4% S77P (TCA→CCA)  SAS0387 → exotoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX571857431,5050TC 7.4% 600.4 / 4.7 174S77P (TCA→CCA) SAS0387exotoxin
Reads supporting (aligned to +/- strand):  ref base T (71/84);  new base C (10/6);  total (82/92)
Fisher's exact test for biased strand distribution p-value = 2.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

AAATGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGACACAT  >  BX571857/431411‑431601
                                                                                              |                                                                                                
aaaTGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAACACCGCAAACa                                                                                                >  2:1599211/1‑97 (MQ=255)
 aaTGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCaa                                                                                               <  2:2358498/97‑1 (MQ=255)
 aaTGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCaa                                                                                               <  1:1804246/97‑1 (MQ=255)
  aTGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCaaa                                                                                              <  1:2222590/97‑1 (MQ=255)
  aTGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCaaa                                                                                              <  2:1570560/97‑1 (MQ=255)
   tGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCAACTAAAGTAGAAGCAccacaaccacaa                                                                                             >  2:1637196/1‑97 (MQ=14)
    gCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAAc                                                                                            <  2:832550/97‑1 (MQ=255)
     caacaaCACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAAcc                                                                                           >  1:103242/1‑97 (MQ=255)
     caacaaCACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAAcc                                                                                           >  1:1764848/1‑97 (MQ=255)
        caacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCaaa                                                                                        >  1:253086/1‑97 (MQ=255)
         aacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGACACAACACAACCACAACCaaca                                                                                       >  2:1338482/1‑95 (MQ=11)
         aacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAc                                                                                       >  1:1997137/1‑97 (MQ=255)
           cacCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcgc                                                                                     <  2:684633/97‑1 (MQ=255)
           cacCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAACCAAAACCAAAcgc                                                                                     >  2:1965868/1‑97 (MQ=255)
            acCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcgcg                                                                                    <  2:2262300/97‑1 (MQ=255)
                tCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGacaa                                                                                >  1:1321946/1‑97 (MQ=255)
                 cTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGacaac                                                                               <  2:2042046/97‑1 (MQ=255)
                 cTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGacaac                                                                               <  1:1207935/97‑1 (MQ=255)
                   tCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCCACTAAAGTAGACACATCGCAATCAAAAACAAAAACGACAacac                                                                             >  2:1155335/1‑97 (MQ=17)
                    cAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACAcc                                                                            <  1:595348/97‑1 (MQ=255)
                      aCTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCAt                                                                          <  1:2305499/97‑1 (MQ=255)
                      aCTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCAt                                                                          <  1:190220/97‑1 (MQ=255)
                       cTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATc                                                                         <  2:2296779/97‑1 (MQ=255)
                        tAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCt                                                                        <  1:226921/97‑1 (MQ=255)
                        tAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCt                                                                        >  1:21440/1‑97 (MQ=255)
                          aaGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTc                                                                      <  1:2402730/97‑1 (MQ=255)
                           aGTGGAAGCACCGCAACAGGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCa                                                                     <  2:387187/97‑1 (MQ=255)
                           aGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCa                                                                     <  1:1170547/97‑1 (MQ=255)
                            gTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCaa                                                                    >  1:1055695/1‑97 (MQ=255)
                            gTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCaa                                                                    <  1:356467/97‑1 (MQ=255)
                             tGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAAc                                                                   >  1:1228569/1‑97 (MQ=255)
                             tGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAAc                                                                   >  1:480508/1‑97 (MQ=255)
                             tGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAAc                                                                   <  2:1159460/97‑1 (MQ=255)
                              ggAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACt                                                                  >  1:800727/1‑97 (MQ=255)
                              ggAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACCCGACAACACCATCTTCAACt                                                                  >  1:197275/1‑97 (MQ=255)
                                aaGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTaa                                                                >  2:774756/1‑97 (MQ=255)
                                aaGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTaa                                                                <  2:227462/97‑1 (MQ=255)
                                 aGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTaaa                                                               >  2:911427/1‑97 (MQ=255)
                                 aGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTaaa                                                               <  2:557385/97‑1 (MQ=255)
                                  gCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAg                                                              >  1:1535802/1‑97 (MQ=255)
                                    aCCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTg                                                            <  1:206141/97‑1 (MQ=255)
                                      cGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGa                                                          >  2:2059688/1‑97 (MQ=255)
                                      cGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGa                                                          <  1:620615/97‑1 (MQ=255)
                                      cGCAACAACCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAAGCAACACCAAACGCGACAACTCCATCTTCAACTAAAGTGGa                                                          <  2:1415548/97‑1 (MQ=18)
                                        caacaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAg                                                        >  2:500339/1‑97 (MQ=255)
                                        caacaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAg                                                        >  1:1209856/1‑97 (MQ=255)
                                         aacaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGc                                                       >  2:247957/1‑97 (MQ=255)
                                            aaacagcaAACGCGACAACACCATCTTCAACTAAAGTGGAACCACAGAAATAAAAACCAAAAGCGACAAAAACATATTAAACTAAAGTGGAAGCAcc                                                    >  2:1111544/4‑97 (MQ=17)
                                                   aaaCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaacaa                                             >  2:1053328/1‑97 (MQ=255)
                                                   aaaCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaacaa                                             >  1:645076/1‑97 (MQ=255)
                                                      cgcgACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAagca                                          >  1:2040053/1‑97 (MQ=255)
                                                       gcgACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAagcag                                         >  2:1012818/1‑97 (MQ=255)
                                                         gACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAgcagca                                       <  1:2084516/97‑1 (MQ=255)
                                                           caacaCCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCaaa                                     <  2:1386859/97‑1 (MQ=255)
                                                           caacaCCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCaaa                                     >  1:1226476/1‑97 (MQ=255)
                                                             acacCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcg                                   >  1:1725042/1‑97 (MQ=255)
                                                             acacCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcg                                   >  2:515795/1‑97 (MQ=255)
                                                              caccacCTTCAACTAAAGTAGAAGCACCGCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcgc                                  <  2:188678/97‑1 (MQ=255)
                                                               acCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcgcg                                 >  1:1463652/1‑97 (MQ=255)
                                                                ccATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGa                                >  2:1355099/1‑97 (MQ=255)
                                                                 cATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGac                               <  1:1091421/97‑1 (MQ=255)
                                                                  aTCTTCAACTAATCTAGAAGCACCGCAATCAAAACCAAACGCAACAACACCATCTCCAACTAAAGTGCAAGCACCGCAACAAGCAGCAAACGCGaca                              >  2:2389888/1‑97 (MQ=18)
                                                                  aTCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGaca                              <  2:2130986/97‑1 (MQ=255)
                                                                  aTCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGaca                              <  1:1651769/97‑1 (MQ=255)
                                                                  aTCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGaca                              <  1:1553544/97‑1 (MQ=255)
                                                                   ccTTCAACTAAAGTAGAAGCACCGCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaa                             <  2:968116/96‑1 (MQ=255)
                                                                   ccTTCAACTAAAGGAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaa                             <  2:1999832/96‑1 (MQ=255)
                                                                    cTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaac                            <  1:1521878/97‑1 (MQ=255)
                                                                    cTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaac                            <  1:768046/97‑1 (MQ=255)
                                                                     ttCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaaca                           >  2:2079976/1‑97 (MQ=255)
                                                                     ttCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaaca                           >  2:1887705/1‑97 (MQ=255)
                                                                       cAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcacc                         >  1:1316904/1‑97 (MQ=255)
                                                                         aCTAAAGTGGAAGCACCGCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcaccac                       <  2:1907791/97‑1 (MQ=255)
                                                                         aCTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcaccac                       <  1:1822467/97‑1 (MQ=255)
                                                                         aCTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcaccac                       <  1:1475043/97‑1 (MQ=255)
                                                                         aCTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcaccac                       <  1:69601/97‑1 (MQ=255)
                                                                          cTAAAGTAGAAGCACCGCAAGAAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACaccacc                      <  2:1785550/97‑1 (MQ=255)
                                                                           tAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAACCGCGACAACACCACCt                     >  2:1395245/1‑97 (MQ=255)
                                                                           tAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCt                     >  1:1583527/1‑97 (MQ=255)
                                                                           tAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCt                     <  1:1640184/97‑1 (MQ=255)
                                                                           tAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCt                     >  1:1955532/1‑97 (MQ=255)
                                                                            aaaGTAGAAGCACCGCAATCAGAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCtt                    <  2:1193086/97‑1 (MQ=255)
                                                                            aaaGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCtt                    <  1:433240/97‑1 (MQ=255)
                                                                             aaGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTc                   >  2:1324208/1‑97 (MQ=255)
                                                                             aaGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTc                   <  1:87915/97‑1 (MQ=255)
                                                                              aGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCg                  <  2:254844/97‑1 (MQ=255)
                                                                              aGTAGAAACACCGCAACCAGAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCg                  <  2:1722581/97‑1 (MQ=255)
                                                                               gTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGt                 <  1:2238219/97‑1 (MQ=255)
                                                                                  gAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTa              >  1:2176965/1‑97 (MQ=255)
                                                                                   aaGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTaa             <  1:2218894/97‑1 (MQ=255)
                                                                                   aaGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTaa             <  1:1359974/97‑1 (MQ=255)
                                                                                      cACCGCAACCAGAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGt          <  2:59909/97‑1 (MQ=255)
                                                                                           cAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGaca     <  1:335391/97‑1 (MQ=255)
                                                                                           cAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGaca     <  1:975917/97‑1 (MQ=255)
                                                                                           cAATCAAAACCAAAAGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGaca     <  2:51688/97‑1 (MQ=255)
                                                                                           cAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGaca     <  1:2278660/97‑1 (MQ=255)
                                                                                            aaTCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGacac    <  1:2256556/97‑1 (MQ=255)
                                                                                            aaTCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGacac    <  1:1599622/97‑1 (MQ=255)
                                                                                             aTCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGAcaca   >  1:1756414/1‑97 (MQ=255)
                                                                                              tCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAAAGTAGACACAt  >  2:1294204/1‑97 (MQ=255)
                                                                                              |                                                                                                
AAATGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGACACAT  >  BX571857/431411‑431601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑