Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 596,994 T→C 8.7% D863D (GAT→GAC SAS0519 → putative surface anchored protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX571857596,9940TC 8.7% 534.8 / 10.2 148D863D (GAT→GACSAS0519putative surface anchored protein
Reads supporting (aligned to +/- strand):  ref base T (73/58);  new base C (2/12);  total (77/71)
Fisher's exact test for biased strand distribution p-value = 3.92e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

TTCAGATACAGATAGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGATTCAGATACAGATAGCGAT  >  BX571857/596898‑597090
                                                                                                |                                                                                                
ttCAGATACAGATAGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCgat                                                                                                  <  2:380869/97‑1 (MQ=255)
 tCAGATACAGATAGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCgatt                                                                                                 >  2:932783/1‑97 (MQ=255)
 tCAGATACAGATAGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCgatt                                                                                                 >  2:1041625/1‑97 (MQ=255)
  cagatacagataGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCgattc                                                                                                >  2:1017597/1‑97 (MQ=255)
  cagatacagataGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCgattc                                                                                                >  2:280289/1‑97 (MQ=255)
  cagatacagataGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCgattc                                                                                                >  1:1863532/1‑97 (MQ=255)
   agatacagataGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCgattca                                                                                               <  2:1907134/97‑1 (MQ=255)
   agatacagataGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCgattca                                                                                               <  2:1849195/97‑1 (MQ=255)
    gatacagataGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCgattcag                                                                                              <  2:852605/97‑1 (MQ=255)
       acagataGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCgattcagatt                                                                                           <  2:300517/97‑1 (MQ=255)
       acagataGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCgattcagatt                                                                                           <  2:1673567/97‑1 (MQ=255)
       acagataGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCgattcagatt                                                                                           <  1:1842984/97‑1 (MQ=255)
        cagataGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCgattcagattc                                                                                          >  2:1442357/1‑97 (MQ=255)
         agataGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCgattcagattca                                                                                         <  2:2434076/97‑1 (MQ=255)
         agataGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCgattcagattca                                                                                         <  2:2188091/97‑1 (MQ=255)
          gataGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGattcagattcag                                                                                        <  1:2245807/97‑1 (MQ=255)
           ataGCGATTCAGGCTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGAttcagattcaga                                                                                       <  1:1495081/97‑1 (MQ=255)
           ataGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGAttcagattcaga                                                                                       <  1:2409809/97‑1 (MQ=255)
           ataGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGAttcagattcaga                                                                                       <  2:2128165/97‑1 (MQ=255)
            taGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGATTCAGATTCAGAc                                                                                      >  1:569199/1‑97 (MQ=255)
             aGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGATTCAGATTCAGACa                                                                                     <  2:1500997/97‑1 (MQ=255)
             aGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGATTCAGATTCAGACa                                                                                     <  2:362716/97‑1 (MQ=255)
              gCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGATTCAGATTCAGACAg                                                                                    >  1:1352905/1‑97 (MQ=255)
                gATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCg                                                                                  >  1:873642/1‑97 (MQ=255)
                gATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCg                                                                                  <  2:811975/97‑1 (MQ=255)
                   tCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACt                                                                               <  1:1158681/97‑1 (MQ=255)
                   tCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACt                                                                               >  2:809361/1‑97 (MQ=255)
                   tCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACt                                                                               >  1:530313/1‑97 (MQ=255)
                     aGACTCGGGTAGCGGCTCAGACGCGGATAGCGAGTCAGACTCAGATAGCGATGCAGACTCAGACTCGGATAGCGATGCAGATGCAGACGGCGACTCa                                                                             <  2:1899779/97‑1 (MQ=21)
                      gACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAg                                                                            <  1:269201/97‑1 (MQ=255)
                       acgcGGAGAGCGAGGCAGACTCGGATAGCGATGCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGa                                                                           <  2:2282839/94‑1 (MQ=25)
                          cGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTc                                                                        >  2:1814848/1‑97 (MQ=255)
                           ggATAGCGACTCTGACTCCGATATGGTTCGAGGCTCAGATAGCGATACAGACTCAGCCTCGGAGAGCGACTCAGATTCAGACAGCGACTCAGATTCg                                                                       <  2:11150/97‑1 (MQ=18)
                           ggATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATCGCGACCCAGACTCAGCCTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCg                                                                       <  2:279270/97‑1 (MQ=25)
                           ggATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCg                                                                       <  1:1812745/97‑1 (MQ=255)
                             aTAGCGACTCAGCCTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGa                                                                     <  2:1046997/97‑1 (MQ=21)
                              tAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACGCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGAc                                                                    >  2:2097913/1‑97 (MQ=255)
                              tAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACCCAGACTCGGATAGCGATTCAGATCCAGACAGCGACTCAGATTCGGAc                                                                    >  2:1113077/1‑97 (MQ=255)
                                gCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAg                                                                  >  1:2260907/1‑97 (MQ=255)
                                 cGACTCAGACTCAGACACCGACTCCGACTCAGACACCGACTCAGACTCAGACTCGGACAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGc                                                                 <  2:1272486/97‑1 (MQ=17)
                                  gACTCCGACTCGGATAGCGAGTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCg                                                                <  1:1889331/97‑1 (MQ=255)
                                   ccgcAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGACGCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGa                                                               <  2:602484/94‑1 (MQ=25)
                                   aCTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGa                                                               <  1:702629/97‑1 (MQ=255)
                                     tCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGAtt                                                             >  1:1243401/1‑97 (MQ=255)
                                          cTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGAc                                                        >  2:1432084/1‑97 (MQ=255)
                                               atagcgattcagactcagatagcgattcagactcagACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGa                                                   <  1:2034197/97‑1 (MQ=255)
                                               atagcgattcagactcagaaagcgattcagactcagACGCGGAGAGCGATGCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGa                                                   <  1:2034190/97‑1 (MQ=21)
                                                tcgcgatgcagtctcagatagcgattcagactcagaTTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGAt                                                  <  1:2417925/95‑1 (MQ=21)
                                                 agcgatacagactcagatggcgattgagactcagaCTCGGAAAGCGAGTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATa                                                 <  1:1360627/97‑1 (MQ=18)
                                                  gcgattcagactcagatagcgattcagactcagaCTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAg                                                >  2:636757/1‑97 (MQ=255)
                                                  gcgattcagactcagatagcgattcagactcagaCTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAg                                                >  1:1150609/1‑97 (MQ=255)
                                                  gcgattcagactcagatagcgattcagactcagaCTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAg                                                >  1:302208/1‑97 (MQ=255)
                                                    gattcagactcagatagcgattcagactcagaCTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCg                                              <  1:652731/97‑1 (MQ=255)
                                                    gattcagactcagatagcgattcagactcagaCTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCg                                              >  1:1214111/1‑97 (MQ=255)
                                                       tcagactcagatagcgattcagactcagaCTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCgact                                           >  1:780604/1‑97 (MQ=255)
                                                       tcagactcagatagcgattcagactcagaCTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCgact                                           >  2:2214826/1‑97 (MQ=255)
                                                       tcagactcagatagcgattcagactcagaCTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCgact                                           >  1:780600/1‑97 (MQ=255)
                                                       tcagactcagatagcgattcagactcagaCTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCgact                                           >  1:1429241/1‑97 (MQ=255)
                                                       tcagactcagatagcgattcagactcagaCTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGAgact                                           >  2:1612345/1‑97 (MQ=255)
                                                        cagactcagatagcgattcagactcagaCTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCgactc                                          >  2:1832107/1‑97 (MQ=255)
                                                            ctcagatagcgattcagactcagaCTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCgactcagac                                      >  2:1198322/1‑97 (MQ=255)
                                                                gatagcgattcagactcagaCTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGactcagactcag                                  <  1:966809/97‑1 (MQ=255)
                                                                  tagcgattcagactcagaCTCGGATAGCGAGTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACACAGATAGCGACTCAGACTCAGaa                                >  1:2225392/1‑97 (MQ=255)
                                                                   agcgattcagactcagaCTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGaaa                               <  1:1668737/97‑1 (MQ=255)
                                                                   agcgattcagactcagaCTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGaaa                               <  1:1018162/97‑1 (MQ=255)
                                                                    gcgattcagactcagaCTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAg                              >  1:874106/1‑97 (MQ=255)
                                                                    gcgattcagactcagaCTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAg                              >  1:1788674/1‑97 (MQ=255)
                                                                     cgatgcagactcagaCTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGt                             <  1:2159545/97‑1 (MQ=255)
                                                                     cgatacagactcagaTTCTGACAGCGACTCAGACTCAGACAGCGATTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGt                             <  2:1553144/97‑1 (MQ=11)
                                                                      gattcagactcagaCTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTg                            >  2:2084865/1‑97 (MQ=255)
                                                                        ttcagactcagaCTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGAt                          >  2:535082/1‑97 (MQ=255)
                                                                          cagactcagactCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGc                        >  1:2318452/1‑97 (MQ=255)
                                                                           agactcagactcGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGcc                       <  1:2327115/97‑1 (MQ=255)
                                                                            gactcagactcaGATAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCg                      <  1:1000336/97‑1 (MQ=255)
                                                                            gactcagactcGGATAGCGATTCGGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCg                      <  1:812439/97‑1 (MQ=255)
                                                                            gactcagactcGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCg                      >  2:1251286/1‑97 (MQ=255)
                                                                             cctccgactcGGACAGCGACTCAGATGCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGa                     <  2:976583/96‑1 (MQ=14)
                                                                             cctcagactcGGACAGCGATTCAGACTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGa                     <  2:2403510/96‑1 (MQ=255)
                                                                              ctcagactcGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGAt                    >  1:1059009/1‑97 (MQ=255)
                                                                              ctcagactcGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGAt                    >  2:1088262/1‑97 (MQ=255)
                                                                               tcagactcGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGAtt                   <  1:2010320/97‑1 (MQ=255)
                                                                                cagactcGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGATTc                  >  1:512284/1‑97 (MQ=255)
                                                                                 agactcGGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGATTca                 <  1:380103/97‑1 (MQ=255)
                                                                                 agacgcGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGATTca                 <  2:2097644/97‑1 (MQ=255)
                                                                                  gactcGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGATTcag                <  1:411753/97‑1 (MQ=255)
                                                                                  gactcGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGATTcag                <  1:2234768/97‑1 (MQ=255)
                                                                                   tctcGGACCGCGACTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGATTcaga               <  2:175829/96‑1 (MQ=14)
                                                                                    ctcGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGATTcagat              >  1:622355/1‑97 (MQ=255)
                                                                                     tcGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGATTcagata             >  1:595894/1‑97 (MQ=255)
                                                                                       ggATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGATTcagataca           >  1:790303/1‑97 (MQ=255)
                                                                                       agacaGCGATTCAGACTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGATTcagataca           <  2:1318335/93‑1 (MQ=255)
                                                                                         aTAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGATTcagatacaga         <  1:1731436/97‑1 (MQ=255)
                                                                                          caGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGATTcagatacagat        <  2:503296/96‑1 (MQ=255)
                                                                                            gCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGATTCAGATACAGATAg      >  1:468536/1‑97 (MQ=255)
                                                                                            gCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGATTCAGATACAGATAg      >  1:422030/1‑97 (MQ=255)
                                                                                            gCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGATTCAGATACAGATAg      >  1:278220/1‑97 (MQ=255)
                                                                                            gCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGATTCAGATACAGATAg      >  1:2180536/1‑97 (MQ=255)
                                                                                             cGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGATTCAGATACAGATAGc     <  1:809521/97‑1 (MQ=255)
                                                                                               attcagattcagACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGATTCAGATACAGATAGCGa   <  1:1105184/97‑1 (MQ=255)
                                                                                                ttcagattcagaCAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGATTCAGATACAGATAGCGAt  >  1:1720511/1‑97 (MQ=255)
                                                                                                |                                                                                                
TTCAGATACAGATAGCGATTCAGACTCGGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGAAAGTGATGCCGATTCAGATACAGATAGCGAT  >  BX571857/596898‑597090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: