Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 605,157 T→C 5.2% D1007D (GAT→GAC SAS0521 → bone sialoprotein‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX571857605,1570TC 5.2% 679.6 / 3.4 182D1007D (GAT→GACSAS0521bone sialoprotein‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (76/93);  new base C (9/3);  total (86/96)
Fisher's exact test for biased strand distribution p-value = 6.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 187 total aligned reads displayed.

TCAGACTCAGATAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGAC  >  BX571857/605062‑605253
                                                                                               |                                                                                                
tcagactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAtt                                                                                                 >  1:2213209/1‑97 (MQ=255)
tcagactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAtt                                                                                                 >  2:160099/1‑97 (MQ=255)
 cagactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTc                                                                                                <  1:993648/97‑1 (MQ=255)
  agactcagaTCGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCa                                                                                               <  2:2331468/97‑1 (MQ=255)
   gactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAg                                                                                              <  1:2019909/97‑1 (MQ=255)
    actcagaAAGTGATTCAGATGCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGa                                                                                             <  1:1101469/97‑1 (MQ=255)
      tcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACa                                                                                           >  1:1674194/1‑97 (MQ=255)
       cagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAg                                                                                          >  2:758676/1‑97 (MQ=255)
        agaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGc                                                                                         <  2:1316264/97‑1 (MQ=255)
         gaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCg                                                                                        <  1:513313/97‑1 (MQ=255)
          aTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCCGACAGCGa                                                                                       >  2:283493/1‑97 (MQ=255)
          aTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGa                                                                                       >  2:2294665/1‑97 (MQ=255)
           tAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAt                                                                                      >  2:1037525/1‑97 (MQ=255)
            aGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAtt                                                                                     <  2:2144578/97‑1 (MQ=39)
            aGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAtt                                                                                     <  2:1445692/97‑1 (MQ=39)
             gTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTc                                                                                    >  1:1699828/1‑97 (MQ=255)
             gTGATTCAGAGTCAGGTAGCGACTCGGACTCAGATAGCCACTCAGACTCGGACAGCGATCCAGACTCTGATAGCGACTCAGATCCAGACACCCATTc                                                                                    >  2:1555116/1‑97 (MQ=11)
               gattcagattcaGATAGCGACTCAGACTCAGATAGGGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                  <  2:425743/97‑1 (MQ=255)
               gattcagattcaGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                  <  1:146904/97‑1 (MQ=255)
               gattcagattcaGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                  <  2:713279/97‑1 (MQ=25)
                attcagattcagATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa                                                                                 >  1:678303/1‑97 (MQ=255)
                 ttcagattcagaTAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                                                                                <  2:923301/97‑1 (MQ=255)
                  tcagattcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACGCAGATAGCGACTCAGATTCAGACAGCGACTCAGACt                                                                               >  2:1938489/1‑97 (MQ=255)
                    agattcatatAGCGACTCAGACTCAGAGAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCa                                                                             <  1:697756/97‑1 (MQ=255)
                    agattcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCa                                                                             <  2:1475622/97‑1 (MQ=255)
                    agattcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCa                                                                             <  1:2193274/97‑1 (MQ=255)
                    agattcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCa                                                                             <  2:1394266/97‑1 (MQ=255)
                    agattcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCa                                                                             <  1:1343234/97‑1 (MQ=255)
                     gattcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAg                                                                            <  1:439870/97‑1 (MQ=255)
                      attcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGa                                                                           <  1:1246738/97‑1 (MQ=255)
                      attcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGa                                                                           <  1:1090580/97‑1 (MQ=255)
                       ttcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                                                                          <  1:480414/97‑1 (MQ=255)
                       ttcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                                                                          <  2:115469/97‑1 (MQ=255)
                        tcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGGTTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATa                                                                         >  1:1330869/1‑97 (MQ=255)
                        tcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATa                                                                         >  2:632230/1‑97 (MQ=255)
                           gatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCg                                                                      <  2:851021/97‑1 (MQ=255)
                            atAGCGACTCAGCCGCAGATAGCGACTCCGATTCGGACAGCGATTCAGGCTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGa                                                                     <  2:1960134/97‑1 (MQ=14)
                             caGCGACTCAGACTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGAc                                                                    <  2:2062838/96‑1 (MQ=255)
                                 gactcagactcaGATAGCGACTCAGAGTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAg                                                                <  2:959531/97‑1 (MQ=255)
                                  actcagactcagATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGa                                                               >  1:838780/1‑97 (MQ=255)
                                  actcagactcagATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGa                                                               <  2:326070/97‑1 (MQ=255)
                                   ctcagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAt                                                              <  1:521870/97‑1 (MQ=255)
                                    tcagactcagaTAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGact                                                             >  2:252455/1‑95 (MQ=9)
                                      agactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCa                                                           <  1:641240/97‑1 (MQ=255)
                                      agactcagaTAGCGACACCGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCa                                                           <  2:442915/97‑1 (MQ=255)
                                        actcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAGTCAGa                                                         >  1:1223847/1‑97 (MQ=255)
                                         ctcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGAc                                                        <  2:1535116/97‑1 (MQ=255)
                                          tcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACa                                                       >  1:2161232/1‑97 (MQ=255)
                                           cagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAg                                                      >  2:2074637/1‑97 (MQ=255)
                                             gaTAGCGACTCAGATTCGGACAGCGATTCCGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTg                                                    <  2:352238/97‑1 (MQ=255)
                                             gaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTg                                                    <  1:601978/97‑1 (MQ=255)
                                             gaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTg                                                    <  1:1743551/97‑1 (MQ=255)
                                              aTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGTTTCAGACAGTga                                                   <  1:956646/97‑1 (MQ=255)
                                               tAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgac                                                  >  2:1422983/1‑97 (MQ=255)
                                                aGCGACTCAGTTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGGTAGCGACGCAGATTCAGACAGTgact                                                 >  1:2396533/1‑97 (MQ=255)
                                                aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgact                                                 >  1:336185/1‑97 (MQ=255)
                                                  cGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactca                                               <  1:2063648/97‑1 (MQ=255)
                                                   gACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcag                                              >  1:1915045/1‑97 (MQ=255)
                                                   gACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcag                                              >  1:1648888/1‑97 (MQ=255)
                                                   gACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcag                                              <  1:2271051/97‑1 (MQ=255)
                                                   gACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcag                                              >  2:1494018/1‑97 (MQ=255)
                                                     cTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCgactcagac                                            >  2:2420405/1‑97 (MQ=25)
                                                       cAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcagactc                                          >  1:503851/1‑97 (MQ=40)
                                                       cAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagactc                                          >  2:1751085/1‑97 (MQ=255)
                                                       cAGATTCAGACAGAGATGCAGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcagactc                                          <  2:1769659/97‑1 (MQ=11)
                                                           ttCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGAt                                      <  1:2427889/97‑1 (MQ=255)
                                                            tCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATa                                     >  1:61086/1‑97 (MQ=255)
                                                              ggACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGc                                   <  1:1535125/97‑1 (MQ=255)
                                                                   gCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCAGATAGCgactc                              >  2:1642090/1‑97 (MQ=11)
                                                                    cGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactca                             >  2:869762/1‑97 (MQ=255)
                                                                     gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcag                            >  1:853247/1‑97 (MQ=255)
                                                                     gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcag                            <  1:925729/97‑1 (MQ=255)
                                                                     gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgacccag                            >  2:1092507/1‑97 (MQ=255)
                                                                       ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcagac                          >  1:169664/1‑97 (MQ=255)
                                                                       ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcagac                          >  2:1732929/1‑97 (MQ=255)
                                                                       ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcagac                          >  2:577563/1‑97 (MQ=255)
                                                                        tcagactcagacAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGa                                                               >  1:339328/1‑59 (MQ=255)
                                                                         cAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcagactc                        >  1:1286095/1‑97 (MQ=255)
                                                                           gACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGactcagactcag                      >  2:1031958/1‑97 (MQ=255)
                                                                             cTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACtcagactcagac                    >  1:1202809/1‑97 (MQ=255)
                                                                               cAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAg                  <  1:1198931/97‑1 (MQ=255)
                                                                                aGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGt                 <  1:1755501/97‑1 (MQ=255)
                                                                                 gATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                <  1:241379/97‑1 (MQ=255)
                                                                                    aGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgact             >  2:948351/1‑97 (MQ=255)
                                                                                    aGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgact             <  2:167306/97‑1 (MQ=255)
                                                                                     gCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactc            >  2:2255521/1‑97 (MQ=255)
                                                                                     gCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactc            >  1:658257/1‑97 (MQ=255)
                                                                                      cGACTCAGATTCCGACAGCGAATCAGACTCAGACAGCGACTCAGACTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactca           <  2:1842119/97‑1 (MQ=14)
                                                                                      cGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactca           <  1:1879186/97‑1 (MQ=255)
                                                                                      cGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactca           <  2:2108707/97‑1 (MQ=255)
                                                                                       gACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcag          <  1:1556901/97‑1 (MQ=255)
                                                                                       gACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcag          <  1:892276/97‑1 (MQ=255)
                                                                                           cAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagactc      >  1:390709/1‑97 (MQ=255)
                                                                                           cAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagactc      >  1:2032895/1‑97 (MQ=255)
                                                                                            aGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagactca     >  2:496185/1‑97 (MQ=255)
                                                                                            aGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagactca     >  1:1425048/1‑97 (MQ=255)
                                                                                            aGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagactca     <  2:1159989/97‑1 (MQ=255)
                                                                                              aTTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTCACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGActcagactcaga   >  2:2044587/1‑97 (MQ=255)
                                                                                               ttCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACtcagactcagac  <  2:2028913/97‑1 (MQ=255)
                                                                                               ttCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACtcagactcagac  >  1:1938070/1‑97 (MQ=255)
                                                                                               |                                                                                                
TCAGACTCAGATAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGAC  >  BX571857/605062‑605253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: