Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 431,505 T→C 9.5% S77P (TCA→CCA)  SAS0387 → exotoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX571857431,5050TC 9.5% 1105.3 / 16.7 311S77P (TCA→CCA) SAS0387exotoxin
Reads supporting (aligned to +/- strand):  ref base T (126/147);  new base C (19/15);  total (146/165)
Fisher's exact test for biased strand distribution p-value = 3.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 315 total aligned reads displayed.

GCAAATGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGACACA  >  BX571857/431409‑431600
                                                                                                |                                                                                               
gCAAATGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAAt                                                                                                 >  1:4128597/1‑97 (MQ=255)
  aaaTGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCa                                                                                               <  1:4535834/97‑1 (MQ=255)
   aaTGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCaa                                                                                              <  1:38614/97‑1 (MQ=255)
   aaTGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCaa                                                                                              <  2:2174035/97‑1 (MQ=255)
   aaTGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCaa                                                                                              <  2:1985592/97‑1 (MQ=255)
    aTGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCaaa                                                                                             <  2:468718/97‑1 (MQ=255)
    aTGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAACACCACAACCaca                                                                                             >  2:2482048/1‑95 (MQ=17)
      gCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCCATCACAAc                                                                                           >  2:1875842/1‑97 (MQ=255)
       caacaaCACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGACGCACCGCAATCAAAAcc                                                                                          >  1:1170903/1‑97 (MQ=255)
        aacaacACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCa                                                                                         <  1:2149792/97‑1 (MQ=255)
        aacaacACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCa                                                                                         <  1:1003450/97‑1 (MQ=255)
         acaacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCaa                                                                                        <  1:4273692/97‑1 (MQ=255)
         acaacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCAACTAAAGTAGACGCACCGCAATCAAAACCaa                                                                                        >  2:4525562/1‑97 (MQ=255)
          caacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCaaa                                                                                       >  1:1081041/1‑97 (MQ=255)
          caacaCCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCAACTAAAGTAGACACACCACCaccaaaaccaaa                                                                                       >  2:1492893/1‑97 (MQ=11)
            acacCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcg                                                                                     >  1:3515940/1‑97 (MQ=255)
            acacCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcg                                                                                     >  1:1590751/1‑97 (MQ=255)
             cacCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcgc                                                                                    <  1:634018/97‑1 (MQ=255)
             cacCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcgc                                                                                    <  2:4374217/97‑1 (MQ=255)
              acCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAAcgcg                                                                                   >  1:4626287/1‑97 (MQ=255)
              acCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAACCAAAACCACAcgcc                                                                                   >  2:4018063/1‑96 (MQ=255)
                cATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGac                                                                                 <  1:3695331/97‑1 (MQ=255)
                 aTCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCAACTAAAGTAGACACACCACCACCAAAACCAAACACcaca                                                                                >  2:1226133/1‑97 (MQ=11)
                  tCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGacaa                                                                               <  2:2247436/97‑1 (MQ=255)
                  tCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGacaa                                                                               <  1:4200059/97‑1 (MQ=255)
                  tCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGacaa                                                                               <  1:2873478/97‑1 (MQ=255)
                   cTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGacaac                                                                              >  1:1882027/1‑97 (MQ=255)
                    ttCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGacaaca                                                                             >  1:4341245/1‑97 (MQ=255)
                       aaCTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCa                                                                          <  1:770288/97‑1 (MQ=255)
                       aaCTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCa                                                                          <  1:1625306/97‑1 (MQ=255)
                        aCTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCAt                                                                         <  1:1543745/97‑1 (MQ=255)
                           aaaGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAACCAAAACCAAACGCGACAACACCATCtt                                                                      >  2:2718761/1‑97 (MQ=255)
                            aaGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTc                                                                     <  1:4431521/97‑1 (MQ=255)
                             aGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCACAACCAAACGCGACAACACCAACTTCa                                                                    >  2:4328556/1‑97 (MQ=255)
                             aGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCa                                                                    <  2:1461627/97‑1 (MQ=255)
                             aGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCa                                                                    <  2:2134031/97‑1 (MQ=255)
                              gTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCaa                                                                   >  2:1110574/1‑97 (MQ=255)
                              gTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCaa                                                                   >  2:4265767/1‑97 (MQ=255)
                                ggAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAAca                                                                 >  2:367328/1‑96 (MQ=255)
                                ggAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACt                                                                 <  2:3053040/97‑1 (MQ=255)
                                ggAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACt                                                                 >  1:2563553/1‑97 (MQ=255)
                                ggAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAACCAAAACCAAACGCGACAACACCATCTTCAAca                                                                 >  2:1632235/1‑96 (MQ=255)
                                 gAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCACACGCGACAACACCATCTTCAACTa                                                                >  1:480997/1‑97 (MQ=255)
                                 gAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTa                                                                >  1:1332946/1‑97 (MQ=255)
                                  aaGCGCCGCGACAAGCAGCAAACGCGACAACACCAGCTGCAACTAAAGGAGGAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTaa                                                               <  1:2903327/97‑1 (MQ=21)
                                  aaGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTaa                                                               >  1:2947397/1‑97 (MQ=255)
                                   aGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTaaa                                                              >  2:1927112/1‑97 (MQ=255)
                                     cACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGt                                                            >  1:2500650/1‑97 (MQ=255)
                                      aCCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTg                                                           <  2:3286932/97‑1 (MQ=255)
                                      aCCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTg                                                           <  2:3227244/97‑1 (MQ=255)
                                        cGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGa                                                         >  2:2765237/1‑97 (MQ=255)
                                        cGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGa                                                         >  2:1060924/1‑97 (MQ=255)
                                          caacaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTGAAGTGGAAg                                                       <  2:3928254/97‑1 (MQ=255)
                                          caacaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAg                                                       <  2:2079623/97‑1 (MQ=255)
                                          caacaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAg                                                       <  2:3845658/97‑1 (MQ=255)
                                          caacaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAg                                                       >  1:317024/1‑97 (MQ=255)
                                            acaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCa                                                     <  2:2681668/97‑1 (MQ=255)
                                            acaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCa                                                     <  2:886101/97‑1 (MQ=255)
                                            acaaGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCa                                                     <  2:1358995/97‑1 (MQ=255)
                                               agcagcAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCg                                                  >  2:3113045/1‑97 (MQ=255)
                                                 cagcaAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGGGGAAGCACCGca                                                >  1:1284551/1‑97 (MQ=255)
                                                  agcaAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaa                                               >  2:804672/1‑97 (MQ=255)
                                                  agcaAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGcaa                                               >  2:2872116/1‑97 (MQ=255)
                                                      aaCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAag                                           >  2:4041207/1‑97 (MQ=255)
                                                       aCGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAagc                                          <  2:719137/97‑1 (MQ=255)
                                                           gACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAgcagca                                      <  1:1902596/97‑1 (MQ=255)
                                                            acaacaCCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCaa                                     <  2:3083094/97‑1 (MQ=255)
                                                            acaacaCCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCaa                                     <  1:2459006/97‑1 (MQ=255)
                                                                 acCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcgcg                                >  1:1432795/1‑97 (MQ=255)
                                                                 acCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcgcg                                <  1:2748477/97‑1 (MQ=255)
                                                                 acCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcgcg                                >  1:2012550/1‑97 (MQ=255)
                                                                 acCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAAcgcc                                >  2:4204771/1‑96 (MQ=255)
                                                                     tCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaa                            <  1:2640466/97‑1 (MQ=255)
                                                                      cTTCAACTAAAGTAGAAGCACCGCAATCAGAACCAAACGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGacaac                           <  2:3788704/97‑1 (MQ=255)
                                                                        tCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAacac                         >  1:1746659/1‑97 (MQ=255)
                                                                        tCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAacac                         >  1:1510380/1‑97 (MQ=255)
                                                                        tCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAacac                         <  1:718023/97‑1 (MQ=255)
                                                                        tCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAacac                         >  1:3757954/1‑97 (MQ=255)
                                                                        tCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAacac                         <  1:4033254/97‑1 (MQ=255)
                                                                        tCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAacac                         <  1:1642442/97‑1 (MQ=255)
                                                                         cAACTAAAGTAGAAGCACCGCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcacc                        <  2:4632984/97‑1 (MQ=255)
                                                                         cAACTAAAGTAGAAACACCGCAATCAAAACCAAACGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACTAGCAGCAAACGCGACAAcacc                        <  1:1570227/97‑1 (MQ=255)
                                                                          aaCTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcacca                       >  2:4141640/1‑97 (MQ=255)
                                                                           aCTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAAcaccac                      <  1:3912776/97‑1 (MQ=255)
                                                                           aCTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACCAAAGCGGAAGCACCGCAACAAGCAGCAAACGCGACAAcaccac                      >  2:1014570/1‑97 (MQ=255)
                                                                             tAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCt                    >  1:1953106/1‑97 (MQ=255)
                                                                             tAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCt                    >  1:1235221/1‑97 (MQ=255)
                                                                             tAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCt                    <  1:211959/97‑1 (MQ=255)
                                                                              aaaGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCtt                   >  2:4540173/1‑97 (MQ=255)
                                                                                agcaGAAGCACCGCAACCAAGACCAAACGCGACAACACCAGCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCg                 <  2:1711164/94‑1 (MQ=17)
                                                                                aGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCg                 <  1:3630158/97‑1 (MQ=255)
                                                                                  tAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTc               >  2:1172527/1‑97 (MQ=255)
                                                                                  tAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTc               >  1:2343919/1‑97 (MQ=255)
                                                                                         aCCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTa        >  1:875352/1‑97 (MQ=255)
                                                                                         aCCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTa        >  1:3362232/1‑97 (MQ=255)
                                                                                           cGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGa      <  1:3226855/97‑1 (MQ=255)
                                                                                           cGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGa      >  1:4062364/1‑97 (MQ=255)
                                                                                           cGCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGa      <  2:614234/97‑1 (MQ=255)
                                                                                            gCAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGac     <  2:346359/97‑1 (MQ=255)
                                                                                             cAACCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGaca    <  2:825485/97‑1 (MQ=255)
                                                                                                |                                                                                               
GCAAATGCAACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCATCTTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACAACACCATCTTCAACTAAAGTGGAAGCACCGCAACAAGCAGCAAACGCGACAACACCACCTTCGTCTAATGTAGACACA  >  BX571857/431409‑431600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑