Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 2,700,167 T→G 5.1% E767D (GAA→GAC SAS2516 ← fibrinogen and keratin‑10 binding surface anchored protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX5718572,700,1670TG 5.1% 1633.8 / 19.0 469E767D (GAA→GACSAS2516fibrinogen and keratin‑10 binding surface anchored protein
Reads supporting (aligned to +/- strand):  ref base T (194/236);  new base G (24/5);  total (226/243)
Fisher's exact test for biased strand distribution p-value = 7.60e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 482 total aligned reads displayed.

GTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAG  >  BX571857/2700071‑2700263
                                                                                                |                                                                                                
  cGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGGTGTCGGAGTCTGAATCGCTGTCTGAtgc                                                                                                >  2:4550820/1‑95 (MQ=17)
   gCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtct                                                                                               >  1:2193723/1‑97 (MQ=255)
     tGTCTGAGTCTGAGTCGCTATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctga                                                                                             <  2:4435744/97‑1 (MQ=255)
      gtctgagtctgaGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCGGAGTCTGAAGCGCTATCTGAGtctggg                                                                                            >  2:236629/1‑95 (MQ=18)
        ctgagtctgagtCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtc                                                                                          <  1:4105157/97‑1 (MQ=255)
        ctgagtctgagtCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtc                                                                                          <  1:1441725/97‑1 (MQ=255)
           agtctgagtcGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgct                                                                                       <  1:2110810/97‑1 (MQ=255)
             tctgagtcGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgt                                                                                     <  2:3510326/97‑1 (MQ=255)
               tgagtcGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtct                                                                                   >  1:3586437/1‑97 (MQ=255)
                     gCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTGTCTGAGtctgagtcgctgtctgagtct                                                                             >  2:3076456/1‑97 (MQ=255)
                      cTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctg                                                                            <  1:3118528/97‑1 (MQ=255)
                      cTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctg                                                                            <  1:2736568/97‑1 (MQ=255)
                       tATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctga                                                                           <  2:1330645/97‑1 (MQ=255)
                        aTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgag                                                                          >  2:3842917/1‑97 (MQ=255)
                        aTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctctctgagtctggg                                                                          >  2:1598904/1‑95 (MQ=255)
                          cTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgagtc                                                                        <  1:1073035/97‑1 (MQ=255)
                          cTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgagtc                                                                        >  1:3231578/1‑97 (MQ=255)
                           tGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgagtcg                                                                       >  1:1841913/1‑97 (MQ=255)
                            gAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgagtcgc                                                                      <  1:712166/97‑1 (MQ=255)
                             agtCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgagtcgct                                                                     <  2:1120614/95‑1 (MQ=255)
                             aaTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgagtcgct                                                                     <  1:1325540/97‑1 (MQ=255)
                              aTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgagtcgctg                                                                    <  1:847802/97‑1 (MQ=255)
                               tCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTGTCTGAAtctgagtcgctgtctgagtctgagtcgctgt                                                                   >  2:4117425/1‑97 (MQ=255)
                               tCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgagtcgctgt                                                                   >  1:473606/1‑97 (MQ=255)
                                cGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgagtcgctgtc                                                                  >  2:1167213/1‑97 (MQ=255)
                                 gCTGTCTGAGTCTGAGTCGCTATNTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagacgctgtctgagtctgagtcgctgtct                                                                 >  1:734934/1‑97 (MQ=255)
                                 gCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgagtcgctgtct                                                                 >  2:293410/1‑97 (MQ=255)
                                 gCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgagtcgctgtct                                                                 <  1:328108/97‑1 (MQ=255)
                                  cTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgagtcgctgtctg                                                                <  1:1024246/97‑1 (MQ=255)
                                  cTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgagtcgctgtctg                                                                <  1:4499622/97‑1 (MQ=255)
                                  cTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgagtcgctgtctg                                                                <  2:3047802/97‑1 (MQ=255)
                                  cTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgagtcgctgtctg                                                                <  2:1039151/97‑1 (MQ=255)
                                   tGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgagtcgctgtctga                                                               >  1:2913666/1‑97 (MQ=255)
                                   tGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgagtcgctgtctga                                                               >  1:4317910/1‑97 (MQ=255)
                                    gtctgagtctgaGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctgagtctgagtcgctgtctgag                                                              >  2:4222354/1‑97 (MQ=255)
                                     tctgagtctgagTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTctgagtcgctgtctgagtctgagtcgctgtctgagt                                                             >  1:4609734/1‑97 (MQ=255)
                                     tctgagtctgagTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTctgagtcgctgtctgagtctgagtcgctgtctgagt                                                             >  1:4230316/1‑97 (MQ=255)
                                     tctgagtctgagTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTctgagtcgctgtctgagtctgagtcgctgtctgagt                                                             <  1:2443092/97‑1 (MQ=255)
                                       tgagtctgagtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTgagtcgctgtctgagtctgagtcgctgtctgagtct                                                           >  2:3388676/1‑97 (MQ=255)
                                       tgagtctgagtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTgagtcgctgtctgagtctgagtcgctgtctgagtct                                                           >  2:212565/1‑97 (MQ=255)
                                        gagtctgagtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGagtcgctgtctgagtctgagtcgctgtctgagtctg                                                          >  1:3355652/1‑97 (MQ=255)
                                         agtctgagtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAgtcgctgtctgagtctgagtcgctgtctgagtctga                                                         <  2:1555645/97‑1 (MQ=255)
                                          gtctgagtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGaa                                                        <  1:3966219/97‑1 (MQ=255)
                                           tctgagtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgc                                                                                        >  1:150795/1‑64 (MQ=37)
                                            ctgagtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATc                                                      >  1:2265995/1‑97 (MQ=255)
                                               agtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCt                                                   >  2:67679/1‑97 (MQ=255)
                                                gtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTg                                                  <  1:296056/97‑1 (MQ=255)
                                                gtcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTg                                                  <  1:1747028/97‑1 (MQ=255)
                                                 tcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCtat                                                 >  1:1222495/1‑95 (MQ=255)
                                                 tcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGt                                                 >  1:3915964/1‑97 (MQ=255)
                                                 tcGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGGCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCtat                                                 >  2:188572/1‑95 (MQ=255)
                                                  cGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATtctgagtcgctgtctg                                                                                  >  1:2319907/1‑63 (MQ=37)
                                                  cGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtc                                                >  2:2897593/1‑97 (MQ=255)
                                                    cTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctg                                              <  2:3185763/97‑1 (MQ=255)
                                                     tATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctga                                             <  2:3305959/97‑1 (MQ=255)
                                                     tATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctga                                             >  2:593166/1‑97 (MQ=255)
                                                      atctgaatctgaGTCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctgaa                                            >  2:2591012/1‑97 (MQ=255)
                                                         tgaatctgaatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctgagtct                                         >  2:2205923/1‑97 (MQ=255)
                                                         tgaatctgaatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctgaatct                                         <  1:154828/97‑1 (MQ=255)
                                                         tgaatctgaatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctgaatct                                         <  1:4479416/97‑1 (MQ=255)
                                                         tgaatctgaatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGtctgagtcc                                         >  2:789836/1‑96 (MQ=11)
                                                         tgaatctgaatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGACTCGCTGTCTGAGTCTGAGTCGCTAtctgaatct                                         >  2:3405218/1‑97 (MQ=255)
                                                          gaatctgaatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctgaatctg                                        <  2:3567798/97‑1 (MQ=255)
                                                           aatctgaatcGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCGGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGtctgaatctga                                       <  2:1832171/97‑1 (MQ=255)
                                                           aatctgaatcGCTGTCTGAGTCTGAATCGCTATCTGACTCTGAGTCGCTGTCTGAGTCTGAGTCGCTCTCCGAGTCTGCATCGCCCgctgattctga                                       >  2:1450655/1‑97 (MQ=14)
                                                             tctgaatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTctgaatctgaat                                     >  2:2016576/1‑97 (MQ=255)
                                                              ctgaatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCtgaatctgaatc                                    <  2:2509615/97‑1 (MQ=255)
                                                              ctgaatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCtgaatctgaatc                                    <  2:3135641/97‑1 (MQ=255)
                                                               tgaatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCg                                   >  1:1686123/1‑97 (MQ=255)
                                                                gaatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGc                                  <  2:539279/97‑1 (MQ=255)
                                                                gaatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGc                                  <  1:3813307/97‑1 (MQ=255)
                                                                 gatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCt                                 <  2:4013787/96‑1 (MQ=255)
                                                                 aatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCt                                 <  2:1415183/97‑1 (MQ=255)
                                                                 aatcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCt                                 <  1:531935/97‑1 (MQ=255)
                                                                   tcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgt                               <  2:227724/97‑1 (MQ=255)
                                                                   tcGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGACTCGCTGTCGGCGTCTGAGTCGCTGGCTGAGTCCGCGTCGCTgt                               >  2:910104/1‑97 (MQ=11)
                                                                     gCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtct                             >  1:3056997/1‑97 (MQ=255)
                                                                        gtctgagtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtctgag                          >  1:3318184/1‑97 (MQ=255)
                                                                        gtctgagtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtctgag                          <  1:3490955/97‑1 (MQ=255)
                                                                        gtctgagtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtctgag                          >  1:2817228/1‑97 (MQ=255)
                                                                             tgtctggGTCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtctgagtctga                     <  2:4366522/96‑1 (MQ=255)
                                                                             agtctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTgtctgagtctga                     <  2:752797/97‑1 (MQ=255)
                                                                              gtctgaATCGCTATCTGATTCGGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGaa                    <  2:1668286/97‑1 (MQ=255)
                                                                                ctgaTTCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATc                  <  1:1157196/97‑1 (MQ=255)
                                                                                ctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATc                  <  1:1935055/97‑1 (MQ=255)
                                                                                ctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATc                  >  2:466169/1‑97 (MQ=255)
                                                                                ctgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATc                  >  2:3930033/1‑97 (MQ=255)
                                                                                 tgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCg                 >  1:2626242/1‑97 (MQ=255)
                                                                                 tgaATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAGTCg                 >  2:364877/1‑97 (MQ=255)
                                                                                  gagtCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGc                <  2:1942687/94‑1 (MQ=255)
                                                                                   aaTCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCt               <  1:366166/97‑1 (MQ=255)
                                                                                    gtCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTa              <  2:2736958/96‑1 (MQ=255)
                                                                                     tCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCtgt             >  2:3787897/1‑95 (MQ=255)
                                                                                      cGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTg                                                  >  1:4177283/1‑59 (MQ=255)
                                                                                       gCTGTCTGGTTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtct           <  2:280282/97‑1 (MQ=255)
                                                                                         tATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCCGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGCCTGAATCGCTAtctga         >  1:3094450/1‑97 (MQ=255)
                                                                                          aTCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctgag        <  1:134447/97‑1 (MQ=255)
                                                                                           tCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCCGAATCGCt                                 >  2:1228608/1‑71 (MQ=255)
                                                                                             tGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctgagtct     <  1:2543831/97‑1 (MQ=255)
                                                                                                ttCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTAtctgagtctgag  <  1:4392842/97‑1 (MQ=255)
                                                                                                |                                                                                                
GTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAG  >  BX571857/2700071‑2700263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: