Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,559,158 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,559,1580AT99.1% 513.0 / NA 222intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base T (103/114);  total (105/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,559,1590GC99.1% 517.9 / NA 222intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (104/115);  minor base A (0/2);  total (105/117)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,559,1600TA99.6% 525.4 / NA 222intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (104/117);  minor base G (1/0);  total (105/117)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 222 total aligned reads displayed.

CGCTGATTGCGCAAAGCTATACCGACAGCTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTAGTCTAAACAACGAGAATGCGGATA‑TT‑AGCAGC‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACAT  >  CP042583/1558883‑1559437
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                        
 gcTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACgag                                                                                                                                                                                                                                                                              >  1:1921235/1‑289 (MQ=255)
   tGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGaa                                                                                                                                                                                                                                                                            <  1:1632129/289‑1 (MQ=255)
     aTTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAAt                                                                                                                                                                                                                                                                           >  2:1980677/1‑288 (MQ=255)
     aTTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAAt                                                                                                                                                                                                                                                                           >  1:387461/1‑288 (MQ=255)
                                        aCCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTcagcag                                                                                                                                                                                                                                    <  1:421584/289‑1 (MQ=255)
                                          cGAATCGGCGGGCTATTTTTAAAAATCTGACTAAACTGTTTTCCCGCGCCCTGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATACCTTCTAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTTGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAgcagca                                                                                                                                                                                                                                  <  1:1371309/289‑1 (MQ=255)
                                          cGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAgcagca                                                                                                                                                                                                                                  <  2:375811/289‑1 (MQ=255)
                                                ggcgggcgATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACg                                                                                                                                                                                                                            <  1:269687/289‑1 (MQ=255)
                                                       gATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGaa                                                                                                                                                                                                                     <  2:1061982/289‑1 (MQ=255)
                                                       gATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTAATTTGTT‑TACTAAACAACGAAAAATGGGATATTT‑CGTTGC‑TTG‑GAGGAAGCATTCAGGAGCAAATaactgggaaat                                                                                                                                                                                                                    >  1:1102912/1‑279 (MQ=255)
                                                         tttttGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAata                                                                                                                                                                                                                   <  1:751875/289‑1 (MQ=255)
                                                              gAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACt                                                                                                                                                                                                              <  2:610522/289‑1 (MQ=255)
                                                                  aTCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAgg                                                                                                                                                                                                          <  1:1998128/289‑1 (MQ=255)
                                                                  aTCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAgg                                                                                                                                                                                                          <  1:113027/289‑1 (MQ=255)
                                                                  aTCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAgg                                                                                                                                                                                                          <  2:69903/289‑1 (MQ=255)
                                                                           accgtttttCCCTCGCCCGGAACTTTACCCAAAAATTAATGTTGCCTTTTATCG‑TTTTGATAAACTCAAATTTATCCAATGTTCGTTTTGGTCATAATATGGGCGTTTTGTTTCCCATTCAGGTTGGCGGCCGCCTTAATACGCTCAG‑GCAAAATGGCGAATTAATTGGTCGTCTCGGCGTTGTTGAG‑TTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGAt                                                                                                                                                                                                <  2:1102912/284‑1 (MQ=255)
                                                                            accgTTTTCCCTCGCCCGGCATCTTAACCATTAGTTAATGTTCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGAt                                                                                                                                                                                                <  2:155185/286‑1 (MQ=255)
                                                                            acTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAACTACGTGGCGAATCTCACTTAGGTGATGTTGAt                                                                                                                                                                                                >  2:1763356/1‑289 (MQ=255)
                                                                            acTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGAt                                                                                                                                                                                                <  1:958794/289‑1 (MQ=255)
                                                                            acTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGAt                                                                                                                                                                                                <  1:1571962/289‑1 (MQ=255)
                                                                                   tCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGtatt                                                                                                                                                                                         >  2:134284/1‑289 (MQ=255)
                                                                                     ccTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGtattat                                                                                                                                                                                       >  2:56434/1‑289 (MQ=255)
                                                                                       tCGCCCTGCATCTTAACCATAGTATAATTATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATTGTGGCCATCTGTTTCTCATTCATGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCTAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATcc                                                                                                                                                                                     <  1:1334660/289‑1 (MQ=255)
                                                                                       tCGCCCGGCATCTTAACCCTAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGAGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATcc                                                                                                                                                                                     <  2:736642/289‑1 (MQ=255)
                                                                                        cGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTACACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTTATGTTGATTTGTATTATccc                                                                                                                                                                                    >  1:907100/1‑289 (MQ=255)
                                                                                          cccGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAGCTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑tttcgt                                                                                                                                                                                                                                                             >  1:1813264/1‑212 (MQ=255)
                                                                                          cccGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAGCTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑tttcgt                                                                                                                                                                                                                                                             <  2:1813264/216‑5 (MQ=255)
                                                                                          cccGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATg                                                                                                                                                                                                     >  1:778053/1‑270 (MQ=255)
                                                                                          cccGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTAATTTATATTATCCggg                                                                                                                                                                                  >  2:1784497/1‑289 (MQ=255)
                                                                                               cATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGcc                                                                                                                                                                              <  1:694661/288‑1 (MQ=255)
                                                                                                 tCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGt                                                                                                                                                                           <  1:1760239/289‑1 (MQ=255)
                                                                                                    tAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGTCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGcc                                                                                                                                                                              <  2:36347/283‑1 (MQ=255)
                                                                                                       ccATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGc                                                                                                                                                                     <  1:1784497/289‑1 (MQ=255)
                                                                                                                aaTGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGaa                                                                                                                                                            >  1:1466002/1‑289 (MQ=255)
                                                                                                                  tGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGAt                                                                                                                                                                                                <  1:890271/251‑1 (MQ=255)
                                                                                                                  tGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGAt                                                                                                                                                                                                >  2:890271/1‑251 (MQ=255)
                                                                                                                   gATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTc                                                                                                                                                         <  1:1684548/289‑1 (MQ=255)
                                                                                                                        cgTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGAt                                                                                                                                                      >  1:1438775/1‑287 (MQ=255)
                                                                                                                        cgTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGAt                                                                                                                                                      >  1:978344/1‑287 (MQ=255)
                                                                                                                            tATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGGGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCGGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGaa                                                                                                                                                >  1:1144160/1‑289 (MQ=255)
                                                                                                                              tCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAaac                                                                                                                                              <  1:626295/289‑1 (MQ=255)
                                                                                                                               gattttGGTTAACTTAAAATTATT‑CATGGTTCGTTTTGGTCAAATTAGTGGGGATCTTTTTCTCATCAAGTTTGGCGGGGGCCTT‑ATAAGCTGCAGGCAAAATGGCGAGGTAATTGGTCTTCTGGTGGGTGATGAGTTTTTGGTTTTTCCACTAAAAACGGAGAAGGCGGTTA‑TT‑TGGTGG‑CTGCGAGAACGCATTCAGCGGCAAATACGTGGGGAATAGCACTTAGTTGAGTTTGATTGGTA‑TATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAaacaa                                                                                                                                            <  2:1592544/284‑1 (MQ=255)
                                                                                                                                gATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAaacaa                                                                                                                                            <  2:680162/289‑1 (MQ=255)
                                                                                                                                  tCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAAcc                                                                                                                                          <  2:81033/289‑1 (MQ=255)
                                                                                                                                           cTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGAc                                                                                                                                 >  2:521223/1‑289 (MQ=255)
                                                                                                                                            ttCAAAATAAT‑CAATGATCGTTTTGGTCATAAGAGTGTGGATCTGTTTCTCATTCAGGTTGGCAAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGCGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACa                                                                                                                                <  2:778529/289‑1 (MQ=255)
                                                                                                                                            gtCAAATTACA‑CTATGATCGTTTTGGTCATAATAGTGGCGATCTGGTTCTCATTCAGGTTGGCGAGCGCCTGCATACGCTCCA‑GCAAGATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACa                                                                                                                                <  2:1133440/288‑1 (MQ=255)
                                                                                                                                                aaTTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑‑T‑ATCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCAAGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTg                                                                                                                             >  2:512287/1‑288 (MQ=255)
                                                                                                                                                      t‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCa                                                                                                                      >  2:1483485/1‑289 (MQ=255)
                                                                                                                                                              tCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCAtgctgc                                                                                                                <  2:1448528/288‑1 (MQ=255)
                                                                                                                                                              tCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATCCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCAtgctgc                                                                                                                <  1:530539/288‑1 (MQ=255)
                                                                                                                                                                        cATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCTAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGa                                                                                                     <  2:1204523/289‑1 (MQ=255)
                                                                                                                                                                        cATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCg                                                                                                      <  1:1483485/288‑1 (MQ=255)
                                                                                                                                                                         ataataGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGAt                                                                                                    >  1:869336/1‑289 (MQ=255)
                                                                                                                                                                           aataGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGATCGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTCATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGATGCTACTCCGGAAACACCAGATGTAGACAGGGTCCTGCACGCTCACGATATTGCGacgc                                                                                                  >  2:1623126/1‑286 (MQ=255)
                                                                                                                                                                                tGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAg                                                                                             >  2:1687208/1‑289 (MQ=255)
                                                                                                                                                                                tGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAg                                                                                             >  1:1230763/1‑289 (MQ=255)
                                                                                                                                                                                 ggCGATCTGTTTCTCATTCAGGTTGGCGAGAGACCTAATACGCTCCA‑GCAAAATGGCGAAGTCATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGATTCACTCAACAACGAGAATGCCGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCTGCAGCAAATACGTGGAGAATCTCACTTAGGTGATATTGCTTTGTATTATCCCGGTGACAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCATGCATGCTGCCGATATTGCGATGTATCAg                                                                                             >  1:1254812/1‑288 (MQ=255)
                                                                                                                                                                                  gCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTAGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCCGCCGATCTTGCGATGTATCAGga                                                                                           >  2:1370841/1‑289 (MQ=255)
                                                                                                                                                                                     aTCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGga                                                                                                                                                                                                                        <  2:1832946/161‑1 (MQ=255)
                                                                                                                                                                                     aTCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAat                                                                                                                                                                                                                    <  1:1950414/165‑1 (MQ=255)
                                                                                                                                                                                     aTCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAat                                                                                                                                                                                                                    >  2:1950414/1‑165 (MQ=255)
                                                                                                                                                                                     aTCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCGCTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCCAAGCAGGAAGTACCCTGCATGCTGCACATGTTGCGATGTATCGGGAGaa                                                                                        >  2:1279790/1‑289 (MQ=255)
                                                                                                                                                                                     aTCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATcc                                                                                                                                                                                     >  1:1406943/1‑196 (MQ=255)
                                                                                                                                                                                     aTCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATcc                                                                                                                                                                                     <  2:1406943/196‑1 (MQ=255)
                                                                                                                                                                                     aTCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGaa                                                                                        >  1:105584/1‑289 (MQ=255)
                                                                                                                                                                                     aTCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGaa                                                                                        >  1:250760/1‑289 (MQ=255)
                                                                                                                                                                                              tcATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACaa                                                                               <  1:579719/289‑1 (MQ=255)
                                                                                                                                                                                                       ttGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGaaa                                                                        <  1:622780/287‑1 (MQ=255)
                                                                                                                                                                                                               cgcCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGc                                                                                                       >  2:1975513/1‑248 (MQ=255)
                                                                                                                                                                                                               cgcCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGc                                                                                                       <  1:1975513/248‑1 (MQ=255)
                                                                                                                                                                                                                gcCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGt                                                                                                                              <  1:1163835/224‑1 (MQ=255)
                                                                                                                                                                                                                gcCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGc                                                                                                       <  1:1650357/247‑1 (MQ=255)
                                                                                                                                                                                                                gcCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTACACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTg                                                              >  1:329113/1‑288 (MQ=255)
                                                                                                                                                                                                                            tCCA‑GCAAACTGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATC‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGAGGGATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCCCATCCac                                                  >  2:1162244/1‑287 (MQ=255)
                                                                                                                                                                                                                            tCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAacac                                                                                 <  1:927074/257‑1 (MQ=255)
                                                                                                                                                                                                                            tCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAgc                                                 >  1:839676/1‑289 (MQ=255)
                                                                                                                                                                                                                            tCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAgc                                                 >  1:1233664/1‑289 (MQ=255)
                                                                                                                                                                                                                            tCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCGGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACCCCTTTTATCGCACATTCatc                                                 >  2:1178705/1‑287 (MQ=255)
                                                                                                                                                                                                                            tCCA‑GCAAAATGGCCAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACCAAACAACGTGAATGCGGATA‑TT‑TCGTCC‑CTGCCAGAACCCATTCAGCAGCAACTACGTGGAGAATATCACTTAGGTGATGTTGATTTATATTATCCCGGTGCCAGTCTTGGCCTCGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCACGCTGCCGACATTGCGATGTATCACGAGAAAAACCACAAACAGAAACCCCCTTTTGTCCCGCATCCAg                                                  >  2:1246600/1‑288 (MQ=255)
                                                                                                                                                                                                                             ccA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAgcg                                                <  2:839676/289‑1 (MQ=255)
                                                                                                                                                                                                                              cA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACAGAAACATAAAACACCTTTTGTCGCGCATCCAgc                                                 >  1:475931/1‑287 (MQ=255)
                                                                                                                                                                                                                                   aaaaTTGCGAAGTTATTGGTGGTCTCGGCGGTGATGGGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTa                                             <  2:1438775/287‑1 (MQ=255)
                                                                                                                                                                                                                                   aaaaTGGCGAAGTTATTGTGCGTTCCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTa                                             <  2:1842668/287‑1 (MQ=255)
                                                                                                                                                                                                                                       tGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGGCCATCCAGCTCTACAtt                                         >  2:1675936/1‑287 (MQ=255)
                                                                                                                                                                                                                                       tGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACAAAAACAAAAAACACCATTTGTCGCGCAAcctctgcacttaaac                                       >  2:623008/1‑276 (MQ=255)
                                                                                                                                                                                                                                         gggAACTTATGGGTCGTCTGGGAGGGGATGAGTTTGTAGGTGTTTAACTCAACAACGAGAATGCGGACA‑TT‑TCGTCG‑CTGAGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATCTCGACTTTTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAAAAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAAAAGAAAACACCATTCGTCGAGCATCCAGCGCTACATTCCt                                      <  2:851711/286‑1 (MQ=255)
                                                                                                                                                                                                                                           gAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAg                                   <  1:731651/289‑1 (MQ=255)
                                                                                                                                                                                                                                            aaGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAg                                   <  2:921229/288‑1 (MQ=255)
                                                                                                                                                                                                                                            aaGTTATTGGCCGCTACGGAGGTGATGAGTTTTTAGTTGTGTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAg                                   <  2:1453417/288‑1 (MQ=255)
                                                                                                                                                                                                                                             atgtcttGGTCGGTTCGGCGGTGATGAGTTTTTAGTTGTTTAACTAAACAACGAGAATGCGGATA‑TT‑TCTTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGc                                 <  2:978344/284‑1 (MQ=255)
                                                                                                                                                                                                                                             aGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTATCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAgg                                  <  1:1597077/288‑1 (MQ=255)
                                                                                                                                                                                                                                                      tcgtcTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTcac                         <  1:142208/288‑1 (MQ=255)
                                                                                                                                                                                                                                                         tctcGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACAt                       >  1:281911/1‑287 (MQ=255)
                                                                                                                                                                                                                                                           tcGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCtt                   >  2:330182/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                ggTGATGGGTTTTTAGTTGTTACACTAAACAACGAGAATGCGGATA‑TTGAGTCG‑‑CGGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGa               <  2:871292/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                    atgaGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAaacaa                                                                                                                                            <  2:1008022/159‑1 (MQ=255)
                                                                                                                                                                                                                                                                    atgaGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAaacaa                                                                                                                                            >  1:1008022/1‑159 (MQ=255)
                                                                                                                                                                                                                                                                        gTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGAt       <  1:373798/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                           tttAGTTGTTTCACTAAACAACGAGAATGCGGATA‑TT‑TCGTCG‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACa   >  1:1508928/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                        
CGCTGATTGCGCAAAGCTATACCGACAGCTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAAT‑CAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCA‑GCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTAGTCTAAACAACGAGAATGCGGATA‑TT‑AGCAGC‑CTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACAT  >  CP042583/1558883‑1559437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑