BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP042583	508,473	3 bp→CGA	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP042583	508,473	0	G	C	99.6%	562.6 / NA	236	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (98/137); minor base A (0/1); total (98/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP042583	508,474	0	C	G	99.6%	553.4 / NA	236	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (97/138); minor base T (1/0); total (98/138)
Fisher's exact test for biased strand distribution p-value = 4.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
*	CP042583	508,475	0	T	A	99.6%	558.9 / NA	236	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (98/137); minor base G (0/1); total (98/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

GGCGATTCCGCTGAGCGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAAAGCACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCGCTCATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑ACTACTA‑CTGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑TGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGGGTGGTGCCACGTTGTTTGGGTT  >  CP042583/508196‑508754

|||

ggcgATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTACTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCgg                                                                                                                                                                                                                                                                                     <  2:2151953/288‑1 (MQ=255)

    aTTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGAtt                                                                                                                                                                                                                                                                                >  2:367308/1‑289 (MQ=255)

         gcgttcggtgtTTTCCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATg                                                                                                                                                                                                                                                                           <  2:375108/282‑1 (MQ=255)

                    tttGCGCCGATCCCTTCCCACATACACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAg                                                                                                                                                                                                                                                                <  2:523536/289‑1 (MQ=255)

                       gcgcCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAgcgc                                                                                                                                                                                                                                                             >  2:1273648/1‑289 (MQ=255)

                       gcgcCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGAAATTGCGGTGATTTGATGGCGCCCACGAgcgc                                                                                                                                                                                                                                                             >  2:1724243/1‑289 (MQ=255)

                             aTCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTAt                                                                                                                                                                                                                                                       >  2:1231810/1‑289 (MQ=255)

                                  ttCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGa                                                                                                                                                                                                                                                  >  1:489298/1‑289 (MQ=255)

                                       acaTAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAg                                                                                                                                                                                                                                              >  1:64271/1‑288 (MQ=255)

                                        caTAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTc                                                                                                                                                                                                                                            >  1:2241410/1‑289 (MQ=255)

                                            aaCTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATg                                                                                                                                                                                                                                                                           <  2:2303108/254‑1 (MQ=255)

                                             aCTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCgcggc                                                                                                                                                                                                                                       >  2:834294/1‑289 (MQ=255)

                                             aCTGATTCGGCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCTGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATGTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCgcggc                                                                                                                                                                                                                                       <  2:2635868/289‑1 (MQ=255)

                                                 aTTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGAt                                                                                                                                                                                                                                    >  1:1401398/1‑288 (MQ=255)

                                                 aTTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATg                                                                                                                                                                                                                                   >  2:721117/1‑289 (MQ=255)

                                                       ttctgtgtggcacagaagGATTGGACGTTGCTGGCAGCAGCGGAACGCCTGGGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAAGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTg                                                                                                                                                                                                                              <  2:340418/275‑1 (MQ=255)

                                                               tGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt                                                                                                                                                                                                                     <  1:1772557/289‑1 (MQ=255)

                                                               tGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt                                                                                                                                                                                                                     <  1:2611697/289‑1 (MQ=255)

                                                               tGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt                                                                                                                                                                                                                     <  1:2121817/289‑1 (MQ=255)

                                                               tGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt                                                                                                                                                                                                                     <  1:2137841/289‑1 (MQ=255)

                                                               tGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt                                                                                                                                                                                                                     <  1:1262961/289‑1 (MQ=255)

                                                               tGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCAGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt                                                                                                                                                                                                                     <  1:1965933/289‑1 (MQ=255)

                                                                 ccAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt                                                                                                                                                                                                                     <  1:263496/287‑1 (MQ=255)

                                                                 ccAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGAGGGCGTCCCAGACCGCGCTTATGACGAATAGTCGGGGCTTGTGGTTGATTACGCGTa                                                                                                                                                                                                                    >  2:1335890/1‑288 (MQ=255)

                                                                    agaagGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt                                                                                                                                                                                                                     >  2:1865926/1‑284 (MQ=255)

                                                                          aatcgaCGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGa                                                                                                                                                                                                           <  1:1889497/283‑1 (MQ=255)

                                                                                   tGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt                                                                                                                                                                                                                     <  1:2716958/269‑1 (MQ=255)

                                                                                               cTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAAt                                                                                                                                                                                           <  2:873971/283‑1 (MQ=255)

                                                                                                      ccTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCg                                                                                                                                                                              <  1:1685692/289‑1 (MQ=255)

                                                                                                         gcgcCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCCCGGCGATCCGTTGATTACGCTGACCTTCCAGACACGTTACCGGCCAATCACCttttggccgtaa                                                                                                                                                                           >  2:2494122/1‑281 (MQ=255)

                                                                                                         gcgcCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCCGTGATTTGATGGCGTCCACGATCGCGATTATGACGGATTGTCGCGTCGATGTGTTTATTACGCGTGCCTTCCAGACAGGTCACCGGCACATCACCTTTTCAGCGTaa                                                                                                                                                                           >  2:1948549/1‑289 (MQ=255)

                                                                                                          cgcCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAAt                                                                                                                                                                          >  1:1372662/1‑289 (MQ=255)

                                                                                                            ccAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACCCGTACCTTCCAGACAGGTCACCGGCACAAAACCTTTTAAGCGTAAttt                                                                                                                                                                        >  2:790677/1‑289 (MQ=255)

                                                                                                                cTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTCCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTTATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGGTGCTTCAGCGTACCTTCCAGACAGGTCAACGGCAAATAAACTTGTCAGCGTAATTTTa‑at                                                                                                                                                                   >  1:1188865/1‑288 (MQ=255)

                                                                                                                 tttcgctattTAACCGTCCAGCCGCGGGAATTTTGACGGTCGGCTAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTa‑at                                                                                                                                                                   <  1:822848/282‑2 (MQ=255)

                                                                                                                       tattTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTa‑atgatgatg                                                                                                                                                            <  2:2105682/289‑9 (MQ=255)

                                                                                                                       tattTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTa‑atgatgat                                                                                                                                                             <  2:1500774/288‑8 (MQ=255)

                                                                                                                          ttAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTa‑atgatgatgag                                                                                                                                                          >  1:1850003/1‑278 (MQ=255)

                                                                                                                           tAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt                                                                                                                                                                                                                     >  2:1036291/1‑229 (MQ=255)

                                                                                                                                 tCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATg                                                                                                                                                                                                                                                                           <  1:599474/169‑1 (MQ=255)

                                                                                                                                 tCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATg                                                                                                                                                                                                                                                                           >  2:599474/1‑169 (MQ=255)

                                                                                                                                          gggAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATg                                                                                                                                       <  1:1946713/289‑1 (MQ=255)

                                                                                                                                           tcagtatggccGGTCGACAAACACCGGTAGCCGGCCGTTAGATTGCTTCTGGCGCAGCAGTTCGTCGCTGTCCATTCCCAGTTCGTTGCCAATTTTATTATCGTCCCATCCGAGCTGCCTAAATTCTCGTACGATTTCCGACGTGGCGGTGATTTGAGGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTTCGCGTACCTTCCAGACAGGTCATCTGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATg                                                                                                                                       <  2:1545974/278‑1 (MQ=255)

                                                                                                                                           ggAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATg                                                                                                                                                                                                                                                      <  1:1512873/180‑1 (MQ=255)

                                                                                                                                           ggAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTa‑atgatgatgagc                                                                                                                                                         <  1:1443778/272‑12 (MQ=255)

                                                                                                                                           ggAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCG‑TGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTa‑at                                                                                                                                                                   >  2:1425102/1‑260 (MQ=255)

                                                                                                                                               tatTGACGGTCGGCAAACACCTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGt                                                                                                                                  <  1:708023/289‑1 (MQ=255)

                                                                                                                                               tatTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGt                                                                                                                                  <  2:2713860/289‑1 (MQ=255)

                                                                                                                                               tatTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACGTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGt                                                                                                                                  <  1:1035492/289‑1 (MQ=255)

                                                                                                                                                     cGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGaaaa                                                                                                                             <  1:2467023/288‑1 (MQ=255)

                                                                                                                                                        tCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCa                                                                                                                          >  2:2660117/1‑288 (MQ=255)

                                                                                                                                                         cGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATCTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGCTGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCAt                                                                                                                         >  1:200243/1‑288 (MQ=255)

                                                                                                                                                           gCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGAtt                                                                                                                                                                                                                                                         >  1:1313897/1‑161 (MQ=255)

                                                                                                                                                           gCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATcc                                                                                                                       >  2:2416233/1‑288 (MQ=255)

                                                                                                                                                                  acTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTa‑atgatgat                                                                                                                                                             >  1:234119/1‑238 (MQ=255)

                                                                                                                                                                  acTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTGCGTCGCTGTCCATTCCCAGCGCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGAACCTTCCAGACAGGTCACCGGCACATGACCTTTTAAGCGTAATTTTATT‑GATGATG‑AT‑CCTTTACCAATTTCCTGCCCGTGAAAACCATCCCCGCttc                                                                                                                >  2:429321/1‑287 (MQ=255)

                                                                                                                                                                    tctTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCa                                                                                                             <  2:1619214/289‑1 (MQ=255)

                                                                                                                                                                      ttGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATg                                                                                                           <  1:2702832/289‑1 (MQ=255)

                                                                                                                                                                             ccGTTAATTTGGTTCAGGTGCAGCACTTCGTCGCTGGCCACTCCCAGCTCGTTGCCAATTTTCCTATCGTCCCATACCAACTGGCTAAGTTCACGGACGATTTCCGACAGGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCAttt                                                                                                     <  2:2257330/288‑1 (MQ=255)

                                                                                                                                                                                ttAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTa                                                                                                  >  1:1582345/1‑288 (MQ=255)

                                                                                                                                                                                   aTTTGTTCCAGGCGAAGAGCTCCGTCTCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCg                                                                                              <  2:2241410/289‑1 (MQ=255)

                                                                                                                                                                                          tCAGGCGCAGAACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTAtgtgt                                                                                       <  1:163777/289‑1 (MQ=255)

                                                                                                                                                                                               cgcAGCAGCTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGGCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGAcc                                                                                   <  2:58983/288‑1 (MQ=255)

                                                                                                                                                                                                  agcaCTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCAttttt                                                                                                   >  2:2472310/1‑269 (MQ=255)

                                                                                                                                                                                                  agcaCTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGa                                                                                     <  1:514548/283‑1 (MQ=255)

                                                                                                                                                                                                      cTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑tggg                                                                          <  1:367308/289‑1 (MQ=255)

                                                                                                                                                                                                      cTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑tggg                                                                          <  1:560826/289‑1 (MQ=255)

                                                                                                                                                                                                        tcgtctCTGTACATTCCCATCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTTAGTTCACGGACGATTTCCGACATGGCCGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑tgggtt                                                                        <  2:1375394/289‑1 (MQ=255)

                                                                                                                                                                                                        tcgtcgCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTa‑at                                                                                                                                                                   >  2:1308745/1‑200 (MQ=255)

                                                                                                                                                                                                        tcgtcgCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTa‑at                                                                                                                                                                   <  1:1308745/201‑2 (MQ=255)

                                                                                                                                                                                                            cgCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑tgggttgggt                                                                    >  2:1675580/1‑289 (MQ=255)

                                                                                                                                                                                                              cTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATTCACGATTTCCATGGCCTTTTTATCCGGATGTTTGACCACAA‑TGGGTTGGGTaa                                                                  >  2:560826/1‑289 (MQ=255)

                                                                                                                                                                                                              cTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCg                                                                                              >  1:1912036/1‑262 (MQ=255)

                                                                                                                                                                                                              cTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCg                                                                                              <  2:1912036/262‑1 (MQ=255)

                                                                                                                                                                                                                gaccATTCCCAGGTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGGTCAAGGACGATTTCCGACATGGCGTTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑TGGGTTGGGTaaaa                                                                <  2:2335800/287‑1 (MQ=255)

                                                                                                                                                                                                                    aTTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑TGGGTTGGGTAAAACCAt                                                            <  1:185928/289‑1 (MQ=255)

                                                                                                                                                                                                                     ttCCCAGCTCTTTGCCAATTTTATTATCGTCCCATACCAACTGGCTAAGTTCACGGACGATTTCCGACATGTCGGTGCTTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATTACCTTTTAAGCGTAATTTTA‑ATGCTTCT‑‑GAGCCTTTATCAATTTCATTGCCGTGATAACCATCCACGTTTTTCTTGGAATTTTTATTCGGATGTGTTACTACAACagggttggacaacacact                                                            >  2:1957445/1‑279 (MQ=255)

                                                                                                                                                                                                                      tCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTATGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATgg                                                                                                                                      >  2:672909/1‑214 (MQ=255)

                                                                                                                                                                                                                           gCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTTACCACAA‑TGGGTTGGGTAAAACCATCTATTTc                                                     >  2:1567770/1‑289 (MQ=255)

                                                                                                                                                                                                                                  gCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCCTCCACGATTTCCATTGCTTTTTTATCCGTATGTTGGACCACCA‑TTGGTTGGTTAACACCATCTATTTCAATGGAt                                              >  2:500943/1‑289 (MQ=255)

                                                                                                                                                                                                                                  gCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑TGGGTTGGGTAAAACCATCTATTTCAATGGAt                                              >  1:58519/1‑289 (MQ=255)

                                                                                                                                                                                                                                                         ccaACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑TGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCggg                       <  1:105468/289‑1 (MQ=255)

                                                                                                                                                                                                                                                           aaCTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑TGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGggtg                     <  1:1020595/289‑1 (MQ=255)

                                                                                                                                                                                                                                                           aaCTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑TGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGggtg                     <  1:790677/289‑1 (MQ=255)

                                                                                                                                                                                                                                                             cTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTT‑‑A‑ATGATTATGAGCCTTTACCAATTTCATTGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATCGGTGACCACAA‑TGGGTTGGGTAAAACCATCTTTTTCAATGGATGTTTGCAGCTGTTTTTTTTCTggtgtt                   >  2:2623813/1‑287 (MQ=255)

                                                                                                                                                                                                                                                                gCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑tggg                                                                          <  1:759656/231‑1 (MQ=255)

                                                                                                                                                                                                                                                                gCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑TGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGGGTGGTGc                 >  2:1178490/1‑288 (MQ=255)

                                                                                                                                                                                                                                                                gCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑TGGGTTGGGTAAAACCATCTATTTCAAGGGATTTTTGCAGCAGTTTTTTTTCGggttgttc                 >  2:2006822/1‑286 (MQ=255)

                                                                                                                                                                                                                                                                  tAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGa                                                                                     >  1:2552885/1‑219 (MQ=255)

                                                                                                                                                                                                                                                                  tAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑TGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGGGTGGTGCCa               <  1:148072/288‑1 (MQ=255)

                                                                                                                                                                                                                                                                  tAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑TGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGGGTGGTGCCa               <  2:631641/288‑1 (MQ=255)

                                                                                                                                                                                                                                                                  tAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑TGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGGGTGGTGCCAc              <  1:1178490/289‑1 (MQ=255)

                                                                                                                                                                                                                                                                    aGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑TGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGGGTGGTGCCACgt            <  1:927195/289‑1 (MQ=255)

                                                                                                                                                                                                                                                                       tCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑TGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGGGTGGTGCCACgttgt         <  1:2416233/289‑1 (MQ=255)

                                                                                                                                                                                                                                                                          cGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACcaca                                                                                <  1:475021/216‑1 (MQ=255)

                                                                                                                                                                                                                                                                          cGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACcaca                                                                                >  2:475021/1‑216 (MQ=255)

                                                                                                                                                                                                                                                                          cGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑TGGGTTGGGTAAAACCa                                                             >  1:1307558/1‑234 (MQ=255)

                                                                                                                                                                                                                                                                          cGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑TGGGTTGGGTAAAACCATCTATTTCaa                                                   <  2:1034243/244‑1 (MQ=255)

                                                                                                                                                                                                                                                                             aCGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑TGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGGGTGGTGCCACGTTGTTTggg    <  1:902916/288‑1 (MQ=255)

                                                                                                                                                                                                                                                                              cGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑TGATGAT‑GAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑TGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGGGTGGTGCCACGTTGTTTGGGtt  >  2:1847327/1‑289 (MQ=255)

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GGCGATTCCGCTGAGCGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAAAGCACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCGCTCATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTA‑A‑ACTACTA‑CTGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAA‑TGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGGGTGGTGCCACGTTGTTTGGGTT  >  CP042583/508196‑508754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`