| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,434,266 | Δ1 bp | 8.0% | coding (125/1248 nt) | PP_1255 ← | cis‑4‑hydroxy‑D‑proline oxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,434,266 | 0 | A | . | 8.0% | 141.6 / 7.7 | 50 | coding (125/1248 nt) | PP_1255 | cis‑4‑hydroxy‑D‑proline oxidase |
| Reads supporting (aligned to +/- strand): ref base A (21/23); new base . (2/2); total (24/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01 | |||||||||||
CATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAGGGCACAGGCGACGCCGACAATGCCGGCGCCCACCACGGCGATATCGGTTTCAGCGGTTTCGACCATGCTCA > NC_002947/1434118‑1434395 | cATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAg > 1:400302/1‑150 (MQ=255) ccTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑gg < 2:500590/150‑1 (MQ=255) ccTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑gg > 1:631049/1‑150 (MQ=255) gCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCt > 1:669151/1‑150 (MQ=255) gCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCC‑GGCCTGGgcgcgt < 2:423588/150‑3 (MQ=255) cAGTTACTTCCAGTCCAGGCGCAGCCGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTg < 1:130183/150‑1 (MQ=255) ccagtccagGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGAc < 1:746271/150‑1 (MQ=255) cagtccagGCGCAGTGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGAcc > 1:462776/1‑150 (MQ=255) tccagGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGc < 2:400302/150‑1 (MQ=255) gCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGAt > 2:164576/1‑150 (MQ=255) cGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATg < 1:164576/150‑1 (MQ=255) ccATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCt > 2:148166/1‑150 (MQ=255) aTCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTgg > 2:560000/1‑150 (MQ=255) tggTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCCATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCgg < 1:175313/149‑1 (MQ=255) ggTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCGGCCT‑GGgcgc < 1:23192/112‑1 (MQ=255) ggTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCC‑GGCCTGGgcgc > 2:23064/1‑112 (MQ=255) ggTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCC‑GGCCTGGgcgc > 2:23192/1‑112 (MQ=255) ggTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCC‑GGCCTGGgcgc < 1:23064/112‑1 (MQ=255) gTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGc > 1:561965/1‑150 (MQ=255) gTCGAGCAGCATGCGAGGCAGGCGCTTGAGGAGCGACAGGTCGGCAATCGGTAATACTTGCTCGGTTGCCAGGTGCCCGGCATTCCAGAACGACATGGTGCGCTC‑GTTCCGTTCGCGGGCGACCACCGTTACCCGATGGCCCTGGCggga > 2:175313/1‑149 (MQ=255) cagcaTGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTc > 1:526468/1‑114 (MQ=255) cagcaTGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTc < 2:526468/114‑1 (MQ=255) cccaTGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGc < 1:142470/148‑1 (MQ=255) gcaTGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGGCGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCa < 2:405637/150‑1 (MQ=255) tGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAggg < 2:631049/150‑1 (MQ=255) gAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCg > 1:143470/1‑133 (MQ=255) gAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCg < 2:143470/133‑1 (MQ=255) ggcaggcGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAGGGCACAg > 2:270830/1‑150 (MQ=255) gcaggcGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAGGGCACAgg < 1:270830/150‑1 (MQ=255) tGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAGGGCACAGGCGACGCCGa < 1:148166/150‑1 (MQ=255) gAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAGGGCACAGGCGACGCCGAc > 2:453874/1‑150 (MQ=255) gATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGGCAGGTGCCCGGCATTGCCGAAGGGGGCGACCAGGCC‑GGGCGCCTGGCGGGCGACAAGAGTTACTCGATGGCCCTGGAGGGCAAGGTGAAGGGAACAGGGGACGCCGAAAAt > 2:309662/1‑150 (MQ=255) aTCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAggg > 1:704929/1‑130 (MQ=255) aTCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAggg < 2:704929/130‑1 (MQ=255) ggCAATCGGGAATACTTGCTGGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAGGGCACAGGCGACGCCGACAATGCCGGCGCccac > 1:161284/1‑150 (MQ=255) cGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAGGGCACAGGCGACGCCGACAATGCCGGCGCCCACCACGGc < 1:227623/150‑1 (MQ=255) gAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAGGGCACAGGCGACGCCGACAATGCCGGCGCCCACCACGGCGat < 2:561965/150‑1 (MQ=255) ttGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGcc < 2:201895/55‑1 (MQ=255) ttGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGcc > 1:201895/1‑55 (MQ=255) gCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAgg < 2:752126/101‑1 (MQ=255) gCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAgg > 1:752126/1‑101 (MQ=255) ggTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAGGGCACAGGCGACGCCGACAATGCCGGCGCCCACCACGGCGATATCGGTTTCAGc > 1:371472/1‑150 (MQ=255) gTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAGGGCACAGGCGACGCCGACAATGCCGGCGCCCACCACGGCGATATCGGTTTCAGCg < 1:560000/150‑1 (MQ=255) cAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAGGGCACAGGCGACGCCGACAATGCCGGCGCCCACCACGGCGATATCGGTTTCAGCGGTTTc < 2:16825/150‑1 (MQ=255) gctGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGCCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAGGGCACAGGCGACGCCGACAATGCCGGCGCCCACCACGGCGATATCGGTTTCAGCGGTTTCGa < 1:309662/148‑1 (MQ=255) cccGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGGCAGTTGCAGGGCACAGGCGCCGGCTACAATGCTGGCGCCCACCACGGCGATATCGGTTTCAGCGGTTTCGACCAt > 1:776588/1‑150 (MQ=255) cccGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAGGGCACAGGCGACGCCGACAATGCCGGCGCCCACCACGGc < 2:416849/123‑1 (MQ=255) cccGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAGGGCACAGGCGACGCCGACAATGCCGGCGCCCACCACGGc > 1:416849/1‑123 (MQ=255) ccGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAGGGCACAGGCGACGCCGACAATGCCGGCGCCCACCACGGCGATATCGGTTTCAGCGGTTTCGACCATg > 2:746945/1‑150 (MQ=255) cATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAGGGCACAGGCGACGCCGACAATGCCGGCGCCCACCACGGCGATATCGGTTTCAGCGGTTTCGACCATGCTCa < 2:462776/150‑1 (MQ=255) | CATGGCCTTGGGCAGGTACTTCCAGTCCAGGCGCAGCGGGCCCATCGGGTCGAGCAGCATGCGAGGCAGGCGCTTGAGGATCGACAGGTCGGCAATCGGGAATACTTGCTCGGTTGCCAGGTGCCCGGCATTGCCGAAGGACGCGCCCAGGCC‑GGGCGCCTGGCGGTCGACCAGCGTTACCCGATGGCCCTGGCGGGCCAGTTGCAGGGCACAGGCGACGCCGACAATGCCGGCGCCCACCACGGCGATATCGGTTTCAGCGGTTTCGACCATGCTCA > NC_002947/1434118‑1434395 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |