| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,263,401 | Δ1 bp | 17.2% | coding (1189/1635 nt) | PP_2861 ← | methyl‑accepting chemotaxis transducer |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,263,401 | 0 | T | . | 17.2% | 77.5 / 12.7 | 29 | coding (1189/1635 nt) | PP_2861 | methyl‑accepting chemotaxis transducer |
| Reads supporting (aligned to +/- strand): ref base T (11/13); new base . (3/2); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01 | |||||||||||
CTTGACCGCAGCGTCCGACTGACTCTGCAGGTGCTCGATCATGGTATGAATCTCGGCCGTCGATTGTGCTGTACGCTGAGCCAACAGTCGCACCTCGTCGGCGACCACGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCGGCAATGGAACGAATCACGTCCAGCACGCCGCCGATGCGCGTGCTGTGGCCGGCCAGGTCGCGGATTACCTGTACGGCCTCATCGATGGTCAGCGACAGGCGGTC > NC_002947/3263252‑3263539 | cTTGACCGCAGCGTCCGACTGACTCTGCAGGTGCTCGATCATGGTATGAATCTCGGCCGTCGATTGTGCTGTACGCTGAGCCAACAGTCGCACCTCGTCGGCGACCACGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGAt > 1:308740/1‑150 (MQ=255) aCCGTAGCGTCCGACTGACTCTGCAGGTGCTCGAGCATGGTATGAATCTCGGCCGTCGATTGTGCTGTACGCTGAGCCAACAGTCGCACCTCGTCGGCGACCACGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATggcg < 1:61358/150‑1 (MQ=255) tCGATCATGGTATGAATCTCGGCTGTCGATTGTGCTGTACGCTGAGCCAACAGTCGCACCTCGTCGGCGACCACGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCg > 2:91418/1‑150 (MQ=255) cGATCATGGTATGAATCTCGGCCGTCGATTGTGCTGTACGCTGAGCCAACAGTCGCACCTCGTCGGCGACCACGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCgg < 2:559502/150‑1 (MQ=255) aTCTCGGCCGTCGATTGTGCTGTACGCTGAGCCAACAGTCGCACCTCGTCGGCGACCACGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCGGCAATGGAACGAATc < 2:737221/150‑1 (MQ=255) tctcGGCCGTCGATTGTGCTGTACGCTGAGCCAACAGTCGCACCTCGTCGGCGACCACGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCGGCAATGGAACGAATCa > 2:94445/1‑150 (MQ=255) ctcGGCCGTCGATTGTGCTGTACGCTGAGCCAACAGTCGCACCTCGTCGGTGCCCTTTTGAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCGGCAATGGAACGAATCAc > 2:669380/1‑150 (MQ=37) tGTACGCTGAGCCAACAGTCGCACCTCGTCGGCGACCACGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCGGCAATGGAACGaa > 1:583379/1‑128 (MQ=255) tGTACGCTGAGCCAACAGTCGCACCTCGTCGGCGACCACGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCGGCAATGGAACGaa < 2:583379/128‑1 (MQ=255) tACGCTGAGCCAACAGTCGCACCTCGTCGGCGACCACGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCGGCAATGGAACGAATCACGTCCAGCACGCCGCCGATgc < 2:308740/150‑1 (MQ=255) gCTGAGCCAACAGTCGCACCTCGTCGGCGACCACGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCGGCAATGGAACGAATCACGTCCAGCACGCCGCCGATGcgcg > 1:510833/1‑150 (MQ=255) ccAACAGTCGCACCTCGTCGGCGACCACGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCCATCGA‑GGCGGCacggg > 2:750942/1‑77 (MQ=18) ccAACAGTCGCACCTCGTCGGCGACCACGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCCATCGA‑GGCGGCacggg < 1:750942/81‑5 (MQ=18) ccAACAGTCGCACCTCGTCGGCGACCACGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCGGCAATGGAACGAATCACGTCCAGCACGCCGCCGATGCGCGTGCtgt < 2:742591/150‑1 (MQ=255) tcgtcgGCGACCACGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCGGCAATGGAACGAATCACGTCCAGCACGCCGCCGATGCGCGTGCTGTGGCCGGCCacagca < 1:91418/150‑6 (MQ=255) cgtcgGCGACCACGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCGGCAATGGAACGAATCACGTCCAGCACGCCGCCGATGCGCGTGCTGTGGCCGGCCAGGTcgc < 2:510833/150‑1 (MQ=255) cACGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCgg < 1:704176/80‑1 (MQ=255) cACGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCgg > 2:704176/1‑80 (MQ=255) cGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCCATCGA‑GGCGGCac < 1:54482/51‑2 (MQ=14) cGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCCATCGA‑GGCGGCac > 2:54482/1‑50 (MQ=14) cGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGt < 2:306918/70‑1 (MQ=255) cGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGt > 1:306918/1‑70 (MQ=255) ccccGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCGGCAATGGAACGAATCACGTCCAGCACGCCGCCGATGCGCGTGCtgtg > 2:427300/1‑116 (MQ=255) ccccGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCGGCAATGGAACGAATCACGTCCAGCACGCCGCCGATGCGCGTGCtgtg < 1:427300/116‑1 (MQ=255) cGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCGGCAATGGAACGAATCACGTCCAGCACGCCGCCGATGCGCGTGCtgt > 2:527813/1‑112 (MQ=255) cGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCGGCAATGGAACGAATCACGTCCAGCACGCCGCCGATGCGCGTGCtgt < 1:527813/112‑1 (MQ=255) cccGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCGGCAATGGAACGAATCACGTCCAGCACGCCGCCGATGCGCGTGCTGTGGCCGGCCAGGTCGCGGATTACCTGTACGGCCTCATCGATGGTCAGCGACAGGc > 1:466594/1‑150 (MQ=255) tGCCGCCGTCGA‑GGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCGGCAATGGAACGAATCACGTCCAGCACGCCGCCGATGCGCGTGCTGTGGCCGGCCAGGTCGCGGATTACCTGTACGGCCTCATCGATGGTCAGCGACAGGCGGTc > 1:116455/1‑150 (MQ=255) gATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCGGCAATGGAACGAATCACGTCCAGCACGCCGCCGATGCGCGTGCTGTGGCCGGCCAGGTCGCGGATTACCTGTACGGCCTCATCGAt > 2:199348/1‑123 (MQ=255) gATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCGGCAATGGAACGAATCACGTCCAGCACGCCGCCGATGCGCGTGCTGTGGCCGGCCAGGTCGCGGATTACCTGTACGGCCTCATCGAt < 1:199348/123‑1 (MQ=255) | CTTGACCGCAGCGTCCGACTGACTCTGCAGGTGCTCGATCATGGTATGAATCTCGGCCGTCGATTGTGCTGTACGCTGAGCCAACAGTCGCACCTCGTCGGCGACCACGGCAAAGCCCCGGCCTTGCTCGCCAGCCCGTGCCGCC‑TCGATGGCGGCATTCAGCGCCAGCAGGTTGGTCTGTTCGGCAATGGAACGAATCACGTCCAGCACGCCGCCGATGCGCGTGCTGTGGCCGGCCAGGTCGCGGATTACCTGTACGGCCTCATCGATGGTCAGCGACAGGCGGTC > NC_002947/3263252‑3263539 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |