| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,644,643 | Δ1 bp | 12.1% | intergenic (+73/‑56) | yhiN → / → PP_4954 | FAD/NAD(P)‑binding domain‑containing oxidoreductase/membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,644,643 | 0 | C | . | 12.1% | 93.6 / 8.8 | 33 | intergenic (+73/‑56) | yhiN/PP_4954 | FAD/NAD(P)‑binding domain‑containing oxidoreductase/membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (12/17); new base . (2/2); total (14/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01 | |||||||||||
CACCGGCCACCTGGGCGGCTTCAACTTCCAGTGGGCTTGGGCATCGGCGAACGCCGCCGCGCAGTTCGTGTAGAGTAGAATCCTTTCAGCAGCACGGAACGCTTCTTGCTGGCCCGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCGCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCGAGCTCGTTCCGTCAGTCACTGCGTCGCCTGTGGGGCCAAGACAAGTTCAGCTACAGCATCCGGGTCACCATTGCC > NC_002947/5644498‑5644782 | cACCGGCCACCTGGGCGGCTTCAACTTCCAGTGGGCTTGGGCATCGGCGAACGCCGCCGCGCAGTTCGTGTAGAGTAGAATCCTTTCAGCAGCACGGAACGCTTCTTGCTGGCCCGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCGccc < 1:134036/150‑1 (MQ=255) aCCGGCCACCTGGGCGGCTTCAACTTCCAGTGGGCTTGGGCATCGGCGAACGCCGCCGCGCAGTTCGTGTGGAGTAGAATCGTTTCAGCAGCCCGGAACGCTTCTTGCTGGCCCGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCGcccc > 1:278451/1‑150 (MQ=255) cGGCCACCTGGGCGGCTTCAACTTCCAGTGGGCTTGGGCATCGGCGAACGCCGCCGCGCAGTTCGTGTAGAGTAGAATCCTTTCAGCAGCACGGAACGCTTCTTGCTGGCCCGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCGCCCCaa < 2:639466/150‑1 (MQ=255) aCTTCCAGTGGGCTTGGGCATCGGCGAACGCCGCCGCGCAGTTCGTGTAGAGTAGAATCCTTTCAGCAGCACGGAACGCTTCTTGCTGGCCCGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCGCCCCAATAACCGGCCCTGAAAGGTAt > 2:499894/1‑150 (MQ=255) ggCATCGGCGAACGCCGCCGCGCAGTTCGTGTAGAGTAGAATCCTTTCAGCAGCACGGAACGCTTCTTGCTGGCCCGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCGCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGcc < 2:191988/150‑1 (MQ=255) gCATCGGCTCAGGCCGCCGCGCAGTTGGTGTAGAGTAGAAGGCTTTCAGCAGCACGGAACGCTTCTTGCTGGCTGGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCGCCCCGAGAACCGGGCCTGAAAGGTATTGTTCTGGTCgccgccg < 2:102160/150‑2 (MQ=255) ggCGAACGCCGCCGCGCAGTTCGTGTAGAGTAGAATCCTTTCAGCAGCACGGAACGCTTCTTGCTGGCCCGTGCTTGGCGCCGTTATTGGGGCGCGCAGCG‑GCCCCAAt < 1:148905/109‑1 (MQ=39) ggCGAACGCCGCCGCGCAGTTCGTGTAGAGTAGAATCCTTTCAGCAGCACGGAACGCTTCTTGCTGGCCCGTGCTTGGCGCCGTTATTGGGGCGCGCAGCG‑GCCCCAAt > 2:148905/1‑109 (MQ=39) cGAACGCCGCCGCGCAGTTCGTGTAGAGTAGAATCCTTTCAGCAGCACGGAACGCTTCTTGCTGGCCCGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCGCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGc < 2:288563/150‑1 (MQ=255) aCGCCGCCGCGCAGTTCGTGTAGAGTAGAATCCTTTCAGCAGCACGGAACGCTTCTTGCTGGCCCGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCGCCCCAATAACCGGc > 2:681728/1‑111 (MQ=255) aCGCCGCCGCGCAGTTCGTGTAGAGTAGAATCCTTTCAGCAGCACGGAACGCTTCTTGCTGGCCCGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCGCCCCAATAACCGGc < 1:681728/111‑1 (MQ=255) gtgtAGAGTAGAATCCTTTCAGCAGCACGGAACGCTTCTTGCTGGCCCGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCGCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCGAGCTCGt > 2:33400/1‑150 (MQ=255) gtctagagtagaAAACTTTCAGCACCAAGGAGCGCTTCTTGCTGGCCCGTGCTTGGCGCCGGTATTAGGGC‑CGCTGCGCGCCCCAATAACCGGCCCGGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCAAGCTCGt < 2:278451/147‑1 (MQ=255) aaTCCTTTCAGCAGCACGGAACGCTTCTTGCTGGCCCGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCGCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCGAGCTCGTTCCgtcagtca > 2:18056/1‑150 (MQ=255) cGGAACGCTTCTTGCTGGCCCGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCGCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCGAGCTCGTTCCGTCAGTCACTGCGTCGCCTGTggg < 1:554965/150‑1 (MQ=255) aaCGCTTCTTGCTGGCCCGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCGCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCGAGCTCGTTCCgtca < 2:396083/127‑1 (MQ=255) aaCGCTTCTTGCTGGCCCGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCGCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCGAGCTCGTTCCgtca > 1:396083/1‑127 (MQ=255) ttcttGCTGGCCCGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCGCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCGAGCTCg < 2:775225/114‑1 (MQ=255) ttcttGCTGGCCCGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCGCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCGAGCTCg > 1:775225/1‑114 (MQ=255) gCTGGCCCGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCGCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATCTCATCGAGCTCGTTCCGTCAGTCACTGCGTCGCCTGTGGGGCCAAGACAAGtt > 1:149985/1‑150 (MQ=255) gCCCGTGTTTGTCGCCGTTATTGGGGC‑CGCTGCGCGCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCGAGCTCGTTCCGTCAGTCACTGCGTCGCCTGTGGGGCCAAGACAAGTTCAGc < 1:33400/150‑1 (MQ=255) gCCCGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCGCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCGAGCTCGTTCCGTCAGTCACTGCGTCGCCTGTGGGGCCAAGACAAGTTCAGc > 1:561146/1‑150 (MQ=255) gCCCGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCGCCACAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCGAGCTCGTTCCGTCAGTCACTGCGTCGCCTGTGGGGCCAAGACAAGTTCAGc < 1:758066/150‑1 (MQ=255) cGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCACCATACCAACCGGCCCTGAAAGGTATTCTTCTCATCACCGACTGGCAGGCCATAATGTCATCGAGCTCGTTCCGTCAGTCACTGCGTCGCCTGTGGGGCCAAGACACGTTCAGCTAc < 1:209362/150‑1 (MQ=255) cGTTATTGGGGC‑CGCTGCGCGCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCGAGCTCGTTCCGTCAGTCACTGCGTCGCCTGTGGGACCAAGACAAGTTCAGCTACAGCATcc < 2:738696/145‑1 (MQ=255) cGTTATTGGGGC‑CGCTGCGCGCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCGAGCTCGTTCCGTCAGTCACTGCGTCGCCTGTGGGACCAAGACAAGTTCAGCTACAGCATcc > 1:738696/1‑145 (MQ=255) gTTATTGGGGC‑CGCTGCGCGCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCGAGCTCGTTCCGTCAGTCACTGCGTCGCCTGTGGGGc > 2:214554/1‑119 (MQ=255) gTTATTGGGGC‑CGCTGCGCGCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCGAGCTCGTTCCGTCAGTCACTGCGTCGCCTGTGGGGc < 1:214554/119‑1 (MQ=255) ttATTGGGGCGCGCAGCG‑GCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCGAGCTCGTTCCGTCAGTCACTGCGTCGCCTGTgggg > 2:94103/1‑117 (MQ=39) ttATTGGGGCGCGCAGCG‑GCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCGAGCTCGTTCCGTCAGTCACTGCGTCGCCTGTgggg < 1:94103/117‑1 (MQ=39) ggC‑CGCTGCGCGCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCGAGCTCGTTCCGTCAGTCACTGCGTCGCCTGTGGGGCCAAGACAAGTTCAGCTACAGCATCCGGGTCACCATTGcc < 1:499894/150‑1 (MQ=255) gCTGCGCGCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCGAGCTCGTTCCGTCAGTCACTGCGTCGCCTGTGGGGCCAAGACAAGTTCAGCTa < 2:705270/124‑1 (MQ=255) gCTGCGCGCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCGAGCTCGTTCCGTCAGTCACTGCGTCGCCTGTGGGGCCAAGACAAGTTCAGCTa > 1:705270/1‑124 (MQ=255) | CACCGGCCACCTGGGCGGCTTCAACTTCCAGTGGGCTTGGGCATCGGCGAACGCCGCCGCGCAGTTCGTGTAGAGTAGAATCCTTTCAGCAGCACGGAACGCTTCTTGCTGGCCCGTGCTTGGCGCCGTTATTGGGGC‑CGCTGCGCGCCCCAATAACCGGCCCTGAAAGGTATTCTTCTGATCACCGCCTGGCAGGCCATCATGTCATCGAGCTCGTTCCGTCAGTCACTGCGTCGCCTGTGGGGCCAAGACAAGTTCAGCTACAGCATCCGGGTCACCATTGCC > NC_002947/5644498‑5644782 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |