| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,661,297 | Δ1 bp | 10.5% | coding (871/1701 nt) | ligB → | DNA ligase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,661,297 | 0 | G | . | 10.5% | 112.6 / 8.8 | 38 | coding (871/1701 nt) | ligB | DNA ligase B |
| Reads supporting (aligned to +/- strand): ref base G (14/20); new base . (2/2); total (16/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.51e-01 | |||||||||||
GACTGGGTTTTCCGGACAGCCAGCTTTACAGCATCGCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAGGGTCACGCCCGTTCTTCACGTGCAAC > NC_002947/5661151‑5661432 | gACTGGGTTTTCCGGACAGCCAGCTTTACAGCATCGCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAgc > 1:39828/1‑150 (MQ=255) ggTTTTCCGGACAGCCAGCTTTACAGCATCGCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑gg < 2:664718/150‑1 (MQ=255) ttttCCGGACAGCCAGCTTTACAGCATCGCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCa > 1:361677/1‑150 (MQ=255) tttCCGGACAGCCAGCTTTACAGCATCGCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAg < 1:140388/150‑1 (MQ=255) cAGCATCGCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGAt < 2:39828/150‑1 (MQ=255) cAGCATCGCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCAAAGGCGCCATACTGGAt < 1:509833/150‑1 (MQ=255) cATCGCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATcgc > 1:163167/1‑150 (MQ=255) aTCGCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATcgag > 1:657112/1‑148 (MQ=255) cGCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGc < 2:361677/150‑1 (MQ=255) gCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCAGAGAAGGACAAGCCCTGCCGCGGGGGACGCGGGCGCCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATAATGGATGGCGGcc > 1:379487/1‑150 (MQ=255) ccATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑gg < 2:763011/119‑1 (MQ=255) ccATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCACTCGGCCACCTGCCGAGCGCT‑gg > 1:763011/1‑119 (MQ=255) gACACGCTGGAGGAAGTAGCACACTGGCGCGAGCACTGGTCTTGATATGCGGGGGTGGTGGTGAGCGTCGTTGTGCTCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAg < 1:692442/150‑1 (MQ=255) tGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCt < 2:152460/150‑1 (MQ=255) tGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCt < 1:204914/150‑1 (MQ=255) gaggaTGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTAt < 2:512095/150‑1 (MQ=255) agcaCACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGc > 1:263911/1‑150 (MQ=255) gcaCACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGcc > 2:76407/1‑150 (MQ=255) gcgcGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGa > 2:644832/1‑150 (MQ=255) cgcgAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGaa < 2:501742/150‑1 (MQ=255) tGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGAc > 2:736288/1‑150 (MQ=255) gTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGt > 1:45577/1‑150 (MQ=255) tCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATcgcg < 2:490541/100‑1 (MQ=255) tCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATcgcg > 1:490541/1‑100 (MQ=255) gctctgcCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCAAAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCgg < 2:379487/150‑1 (MQ=255) cTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCg < 1:76407/150‑1 (MQ=255) ccACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTg < 2:680025/150‑1 (MQ=255) aCCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGc < 2:163167/150‑1 (MQ=255) aTCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGAGCG‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAGGGTCACGccc > 1:461621/1‑150 (MQ=255) gcCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTCCGGGTCGGCCGCACTGGCAGGGTCACGCCCGttcttc < 1:644832/150‑1 (MQ=255) gcCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGAGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAGGGTCACGCCCGttcttc < 2:657112/150‑1 (MQ=255) gggCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAGGGTCACGcc < 2:283814/138‑1 (MQ=255) gggCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAGGGTCACGcc > 1:283814/1‑138 (MQ=255) gTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAGGGTCACGCCCGTTCTTCACGTGCAAc < 1:736288/150‑1 (MQ=255) ttggccTGCC‑AGCGCTCGGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAggg < 1:521503/118‑1 (MQ=255) ttggccTGCC‑AGCGCTCGGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAggg > 2:521503/5‑122 (MQ=255) ttggccTGCC‑AGCGCTCGGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAggg > 2:521544/5‑122 (MQ=255) gtgtccTGCC‑AGCGCTCGGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAggg < 1:521544/118‑1 (MQ=255) gAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAg > 2:792480/1‑61 (MQ=255) gAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAg < 1:792480/61‑1 (MQ=255) | GACTGGGTTTTCCGGACAGCCAGCTTTACAGCATCGCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAGGGTCACGCCCGTTCTTCACGTGCAAC > NC_002947/5661151‑5661432 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |