Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,337,837:1 +T 9.8% coding (998/1047 nt) PP_2934 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,337,8371.T 9.8% 147.5 / 9.9 41coding (998/1047 nt)PP_2934hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (19/18);  new base T (2/2);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGCCCACTGCGATGCCGAGCAGTACGCGCGAATGCCGGCGCTGTGCAAGGACGCCGACGGCGTCGACCGCCGGTATATCCATCATTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAGGGCAGCCACGCAGAAGATCGAGGCATACCCCAGGTGTACCGCCACGGCGCCCATCACCGGGCCGGCAATGGCCAA  >  NC_002947/3337695‑3337985
                                                                                                                                               |                                                                                                                                                    
aTGGCCCACTGCGATGCCGAGCAGTACGCGCGAATGCCGGCGCTGTGCAAGGACGCCGACGGCGTCGACCGCCGGTATATCCATCATTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAg                                                                                                                                               >  2:725807/1‑150 (MQ=255)
aTGGCCCACTGCGATGCCGAGCAGTACGCGCGAATGCCGGCGCTGTGCAAGGACGCCGACGGCGTCGACCGCCGGTATATCCATCATTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCC‑GGCGCTGGCGACCCCAGCGCCTGGCTGaa                                                                                                                                               <  1:733259/150‑2 (MQ=255)
      cACTGCGATGCCGAGCAGTACGCGCGAATGCCGGCGCTGTGCAAGGACGCCGACGGCGTCGACCGCCGGTATATCCATCATTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCa                                                                                                                                         <  1:346526/150‑1 (MQ=255)
           cGATGCCGAGCAGTACGCGCGAATGCCGGCGCTGTGCAAGGACGCCGACGGCGTCGACCGCCGGTATATCCATCATTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGc                                                                                                                                    <  2:79637/150‑1 (MQ=255)
                  gAGCAGTACGCGCGAATGCCGGCGCTGTGCAAGGACGCCGACGGCGTCGACCGCCGGTATATCCATCATTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGa                                                                                                                                                >  2:90428/1‑131 (MQ=255)
                  gAGCAGTACGCGCGAATGCCGGCGCTGTGCAAGGACGCCGACGGCGTCGACCGCCGGTATATCCATCATTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGa                                                                                                                                                <  1:90428/131‑1 (MQ=255)
                   aGCAGTACGCGCGAATGCCGGCGCTGTGCAAGGACGCCGACGGCGTCGACCGCCGGTATATCCATCATTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGgcg                                                                                                                            <  1:615803/150‑1 (MQ=255)
                        tACGCGCGAATGCCGGCGCTGTGCAAGGACGCCGACGGCGTCGACCGCCGGTATATCCATCATTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCGGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGcgcgccg                                                                                                                       >  2:18971/1‑150 (MQ=255)
                           gcgcgAATGCCGGCGCTGTGCAAGGACGCCGACGGCGTCGACCGCCGGTATATCCATCATTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGcgcgccgcgc                                                                                                                    >  2:93284/1‑150 (MQ=255)
                           gcgcgAATGCCGGCGCTGTGCAAGGACGCCGACGGCGTCGACCGCCGGTATATCCATCATTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGcgcgccgcgc                                                                                                                    >  1:348795/1‑150 (MQ=255)
                                         gcTGTGCAAGGACGCCGACGGCGTCGACCGCCGGTATATCCATCATTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTa                                                                                                      <  1:664691/150‑1 (MQ=255)
                                           tgtgCAAGGACGCCGACGGCGTCGACCGCCGGTATATCCATCATTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAgg                                                                                                    >  2:159965/1‑150 (MQ=255)
                                                      gCCGACGGCGTCGACCGCCGGTATATCCATCATTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGc                                                                                         <  2:348795/150‑1 (MQ=255)
                                                        cGACGGCGTCGACCGCCGGTATATCCATCATTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCa                                                                                       >  2:165/1‑150 (MQ=255)
                                                                tCGACCGCCGGTATATCCATCATTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGc                                                                               >  2:368039/1‑150 (MQ=255)
                                                                  gACCGCCGGTATATCCATCATTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAg                                                                             >  2:10063/1‑150 (MQ=255)
                                                                   aCCGCCGGTATATCCATCATTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAgg                                                                            >  2:522681/1‑150 (MQ=255)
                                                                                 catcatTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGc                                                                                         <  1:656096/123‑1 (MQ=255)
                                                                                 catcatTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGc                                                                                         >  2:656096/1‑123 (MQ=255)
                                                                                  atcatTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAGGGCAGCCACGCagaag                                                             >  1:574222/1‑150 (MQ=255)
                                                                                   tcatTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTGAGCCGACCCCGGCCAAGGCGAGCAGGGCAGCCACGCagaaga                                                            >  1:7718/1‑150 (MQ=255)
                                                                                      ttCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGc                                                                                         <  1:673009/118‑1 (MQ=255)
                                                                                      ttCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGc                                                                                         >  2:673009/1‑118 (MQ=255)
                                                                                         gCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAGGGCAGCCACGCAGAAGATCGAgg                                                      >  1:757281/1‑150 (MQ=255)
                                                                                         gCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAGGGCAGCCACGCAGAAGATCGAgg                                                      <  1:692218/150‑1 (MQ=255)
                                                                                           tGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAGGGCAGCCACGCAGAAGATCGAGGCa                                                    <  1:349718/150‑1 (MQ=255)
                                                                                                   gaAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAGGGCAGCCACGCAGAAGATCGAGGCATACCCCAg                                            <  1:368039/150‑1 (MQ=255)
                                                                                                     aaCGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAGGGCAGCCACGCAGAAGATCGAGGCATACCCCAGgt                                          <  1:332429/150‑1 (MQ=255)
                                                                                                         gCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAGGGCAGCCACGCAGAAGATCGAGGCATACCCCAGGTGTAc                                      <  1:165/150‑1 (MQ=255)
                                                                                                          cAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAGGGCAGCCACGCAGAAGATCGAGGCATACCCCAGGTGTAcc                                     >  2:390876/1‑150 (MQ=255)
                                                                                                                  ttcAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAGGGCATCCACGCAGAAGATCGAGGCATACCCCAGGTGTACCGCCACGgc                             <  2:577607/150‑1 (MQ=255)
                                                                                                                    tcagcAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAGGGCAGCTACGCAGAAGATCGa                                                        <  2:430105/117‑1 (MQ=255)
                                                                                                                    tcagcAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAGGGCAGCTACGCAGAAGATCGa                                                        >  1:430105/5‑121 (MQ=255)
                                                                                                                     aGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCATGGCATCCACGCAGAAGATCGAGGCATACCCCAGGTGTACCGCCACGGCGcc                          >  2:688616/1‑150 (MQ=255)
                                                                                                                        ccggCGCTGGCGACCCCAGCGCCTGGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAGGGCAGCCACGCAGAAGATCGAGGCATACCCCAGGTGTACCGCCACGGCGCCCa                        >  2:213730/3‑150 (MQ=255)
                                                                                                                        ccggCGCTGGCGACCCCAGCGCCTGGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACACCGGCCAAGGCGAGCAGGGCAGCCACGCAGAAGATCGAGGCATACCCCAGGTGTACCGCCACGGCGCCCa                        >  2:89281/3‑150 (MQ=255)
                                                                                                                         aGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAGGGCAGCCACGCAGAAGATCGAGGCATACCCCAGGTGTACCGCCACGGCGCCCATc                      >  2:251231/1‑150 (MQ=255)
                                                                                                                                   gtggCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAGGGCCGCCACGCAGCAGATCGAGGCATAACCCAGGTGTACCGCCTCGGCGCCCATGACCGGGCCgg            <  2:50507/147‑1 (MQ=255)
                                                                                                                                       ccAGCGCCTGGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAGGGCAGCCACGCAGAAGATCGAGGCATACCCCAGGTGTACCGCCACGGCGCCCATCACCGGGCCGGCaa         <  1:213730/150‑1 (MQ=255)
                                                                                                                                       ccAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAGGGCAGCCACGCAGAAGATCGAGGCATACCCAAGGTGTACCGCCACGGCGCCCATCACCGGGCCGGCAAt        <  2:56241/150‑1 (MQ=255)
                                                                                                                                         aGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAGGGCAGCCACGCAGAAGATCGAGGCATACCCCAGGTGTACCGCCACGGCGCCCATCACCGGGCCGGCAATgg      <  2:574222/150‑1 (MQ=255)
                                                                                                                                             cc‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAGGGCAGCCACGCAGAAGATCGAGGCATACCCCAGGTGTACCGCCACGGCGCCCATCACCGGGCCGGCAATGGCCaa  <  1:159965/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
ATGGCCCACTGCGATGCCGAGCAGTACGCGCGAATGCCGGCGCTGTGCAAGGACGCCGACGGCGTCGACCGCCGGTATATCCATCATTCGCTGCAGCGAGAAACGGCAGCGTTCTTCAGCCAGGCGCTGGGGTCGCCAGCGCC‑GGCTGAGCGTTCAGCTGCGACGGGCGCGCCGCGCCAGCCACAACGTTAGGCCGACCCCGGCCAAGGCGAGCAGGGCAGCCACGCAGAAGATCGAGGCATACCCCAGGTGTACCGCCACGGCGCCCATCACCGGGCCGGCAATGGCCAA  >  NC_002947/3337695‑3337985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑