| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,842,949:1 | +G | 16.4% | intergenic (+48/‑249) | ccoP‑II → / → PP_4259 | cbb3‑type cytochrome c oxidase subunit/iron‑sulfur cluster‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,842,949 | 1 | . | G | 16.4% | 164.8 / 34.1 | 55 | intergenic (+48/‑249) | ccoP‑II/PP_4259 | cbb3‑type cytochrome c oxidase subunit/iron‑sulfur cluster‑binding protein |
| Reads supporting (aligned to +/- strand): ref base . (25/21); new base G (4/5); total (29/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
GATGCCGGCCCAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAACCCATACTGCCAT > NC_002947/4842799‑4843098 | gATGCCGGCCCAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑AGGATCCt < 1:774393/150‑1 (MQ=255) cGGCCCAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTa > 1:241166/1‑150 (MQ=255) ccAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAg < 1:240008/150‑1 (MQ=255) aGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTAAAAGGCATTGATAAACAATACT‑TGGCTGCCAAGGCTGATGAAGTCAGGCCTGGACTCTTCGCGGGTAAAGCCGCTCCTACACAGGATCCT‑TGTAGGAGCg < 1:779362/150‑1 (MQ=255) ggAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑AGGATCCTGTGTAGGAGCgg < 2:707875/150‑1 (MQ=255) gAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑AGGATCCTGTGTAGGAGCggg < 1:313245/150‑1 (MQ=255) gCAACGACAAGCTGCACTTGCTGGCGGCTTACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCACTCCTACACAGGATCCT‑TGTCGGAGCGGGTTTACCCGCGagg > 2:793517/1‑148 (MQ=255) cAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAaga < 1:691854/150‑1 (MQ=255) aCGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAgg < 1:113453/150‑1 (MQ=255) cGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGc < 2:241166/150‑1 (MQ=255) gACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGTATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGcc > 2:647136/1‑150 (MQ=255) gACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑AGGATCCTGTGTAGGAGCgg < 1:128159/131‑1 (MQ=255) gACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑AGGATCCTGTGTAGGAGCgg > 2:128159/1‑131 (MQ=255) aGGTGCACTTGCTGGCGGCTTACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTa > 2:705443/1‑150 (MQ=255) ttGCTGGCGGCTTACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTAccc < 1:487871/127‑1 (MQ=255) ttGCTGGCGGCTTACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTAccc > 2:487871/1‑127 (MQ=255) tGGCGGCTTACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGc < 1:682365/148‑1 (MQ=255) tGGCGGCTTACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGc > 2:682365/1‑148 (MQ=255) tGGCGGCTTACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCAt > 2:323521/1‑150 (MQ=255) gcggcTTACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCAttt > 2:678838/1‑150 (MQ=255) ttACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTAc > 2:788544/1‑132 (MQ=255) ttACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTAc < 1:788544/132‑1 (MQ=255) cAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTtctc < 1:196308/139‑1 (MQ=255) cAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTtctc > 2:196308/1‑139 (MQ=255) gC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGCTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACGGGCAGAGCATTTCTCGCAGGCAAGTGAc > 2:299737/1‑150 (MQ=255) c‑cTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGAcc < 1:323521/150‑1 (MQ=255) cACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGCATTCT‑GGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCa > 1:450482/1‑150 (MQ=255) ccAGGCTGAGCCGGCCAAGACTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCaa > 1:277853/1‑149 (MQ=255) ccAGGCTGAGCCGGCCAAGACTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCaa < 2:277853/149‑1 (MQ=255) cGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCg < 1:69676/96‑1 (MQ=255) cGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCg > 2:69676/1‑96 (MQ=255) ggCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATAGCGCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAacac > 2:688845/1‑150 (MQ=255) gCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACg > 1:274717/1‑150 (MQ=255) cTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACCCAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTCCTCGGAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGt < 1:299737/150‑1 (MQ=255) cTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGt > 1:446030/1‑150 (MQ=255) tGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGtt < 1:678838/150‑1 (MQ=255) caggcCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCAc < 2:450482/150‑1 (MQ=255) ccgatctctctTCGCGGGTAACCCCGCTCTTACGGAGGGTCCT‑TGTAGGAGCGGGTTTTCCGGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACCCCCGCCATAACACGTTTCATACCACGaa < 2:48815/143‑1 (MQ=37) tctTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGTCGGTGATCAATTGACACCCGCCATAACACGGTTCATACCACGAAcagatcgg > 1:48815/1‑143 (MQ=37) aaCCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACTTTTCATACCACGAACCCAACGCGACTAAAGGt > 2:797086/1‑148 (MQ=255) aaCCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACTTTTCATACCACGAACCCAACGCGACTAAACGt < 1:797086/148‑1 (MQ=255) ccGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAg > 1:404650/1‑150 (MQ=255) ccGCTCCTACA‑AGGATCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAg > 2:97063/1‑150 (MQ=255) ctcTCATACACACGAGCCT‑TGTAGGAGCGGGTTTACCCGCGAAGACCCGGGCAGTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGt < 1:647136/148‑1 (MQ=255) gCTCCTACA‑AGGATCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAAtt > 2:547575/1‑93 (MQ=21) gCTCCTACA‑AGGATCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTg > 1:310806/1‑150 (MQ=255) gCTCCTACA‑AGGATCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTGCTCGCAGGCAAGTGACCTGGATCAAtt < 1:547575/93‑1 (MQ=21) cTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTCTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGaa < 1:705443/150‑1 (MQ=255) tACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAAc > 1:459594/1‑150 (MQ=255) ctacagggATCCTGTGTAGGAGCGAGTTTACCCGGGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGaa < 2:310806/144‑1 (MQ=255) acacAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAAcc > 2:438010/1‑150 (MQ=255) acaGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAAt < 1:797625/84‑1 (MQ=255) acaGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAAt > 2:797625/1‑84 (MQ=255) acaGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCAc > 2:304947/1‑114 (MQ=255) acaGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCAc < 1:304947/114‑1 (MQ=255) acaGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACCGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAACCCa > 2:157141/1‑150 (MQ=255) t‑tGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAACCCATACTGCCAt > 2:465316/1‑150 (MQ=255) | GATGCCGGCCCAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGC‑CTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAACCCATACTGCCAT > NC_002947/4842799‑4843098 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |