Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,661,303:1	+C	11.1%	coding (877/1701 nt)	ligB →	DNA ligase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,661,303	1	.	C	11.1%	124.0 / 10.0	36	coding (877/1701 nt)	ligB	DNA ligase B
Reads supporting (aligned to +/- strand):  ref base . (13/19);  new base C (2/2);  total (15/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTTTTCCGGACAGCCAGCTTTACAGCATCGCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAGGGTCACGCCCGTTCTTCACGTGCAAC  >  NC_002947/5661156‑5661432                                                                                                                                                     |                                                                                                                                  ggTTTTCCGGACAGCCAGCTTTACAGCATCGCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑gg                                                                                                                                 <  2:664718/150‑1 (MQ=255)   ttttCCGGACAGCCAGCTTTACAGCATCGCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCa                                                                                                                               >  1:361677/1‑150 (MQ=255)    tttCCGGACAGCCAGCTTTACAGCATCGCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAg                                                                                                                              <  1:140388/150‑1 (MQ=255)                        cAGCATCGCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGAt                                                                                                          <  2:39828/150‑1 (MQ=255)                        cAGCATCGCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCAAAGGCGCCATACTGGAt                                                                                                          <  1:509833/150‑1 (MQ=255)                           cATCGCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATcgc                                                                                                       >  1:163167/1‑150 (MQ=255)                            aTCGCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATcgag                                                                                                      >  1:657112/1‑148 (MQ=255)                              cGCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGc                                                                                                    <  2:361677/150‑1 (MQ=255)                               gCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCAGAGAAGGACAAGCCCTGCCGCGGGGGACGCGGGCGCCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATAATGGATGGCGGcc                                                                                                   >  1:379487/1‑150 (MQ=255)                                ccATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑gg                                                                                                                                 <  2:763011/119‑1 (MQ=255)                                ccATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCACTCGGCCACCTGCCGAGCGCT‑gg                                                                                                                                 >  1:763011/1‑119 (MQ=255)                                     gACACGCTGGAGGAAGTAGCACACTGGCGCGAGCACTGGTCTTGATATGCGGGGGTGGTGGTGAGCGTCGTTGTGCTCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAg                                                                                             <  1:692442/150‑1 (MQ=255)                                            tGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCt                                                                                      <  1:204914/150‑1 (MQ=255)                                            tGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCt                                                                                      <  2:152460/150‑1 (MQ=255)                                              gaggaTGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTAt                                                                                    <  2:512095/150‑1 (MQ=255)                                                      agcaCACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGc                                                                            >  1:263911/1‑150 (MQ=255)                                                       gcaCACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGcc                                                                           >  2:76407/1‑150 (MQ=255)                                                               gcgcGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGa                                                                   >  2:644832/1‑150 (MQ=255)                                                                cgcgAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGaa                                                                  <  2:501742/150‑1 (MQ=255)                                                                         tGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGAc                                                         >  2:736288/1‑150 (MQ=255)                                                                           gTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGt                                                       >  1:45577/1‑150 (MQ=255)                                                                              tCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATcgcg                                                                                                      <  2:490541/100‑1 (MQ=255)                                                                              tCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATcgcg                                                                                                      >  1:490541/1‑100 (MQ=255)                                                                                     gctctgcCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCAAAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCgg                                             <  2:379487/150‑1 (MQ=255)                                                                                             cTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCg                                     <  1:76407/150‑1 (MQ=255)                                                                                                  ccACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTg                                <  2:680025/150‑1 (MQ=255)                                                                                                    aCCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGc                              <  2:163167/150‑1 (MQ=255)                                                                                                                aTCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGAGCG‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAGGGTCACGccc                  >  1:461621/1‑150 (MQ=255)                                                                                                                       gcCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTCCGGGTCGGCCGCACTGGCAGGGTCACGCCCGttcttc           <  1:644832/150‑1 (MQ=255)                                                                                                                       gcCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGAGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAGGGTCACGCCCGttcttc           <  2:657112/150‑1 (MQ=255)                                                                                                                           gggCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAGGGTCACGcc                   <  2:283814/138‑1 (MQ=255)                                                                                                                           gggCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAGGGTCACGcc                   >  1:283814/1‑138 (MQ=255)                                                                                                                                gTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAGGGTCACGCCCGTTCTTCACGTGCAAc  <  1:736288/150‑1 (MQ=255)                                                                                                                                    ttggccTGCC‑AGCGCTCGGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAggg                          <  1:521503/118‑1 (MQ=255)                                                                                                                                    ttggccTGCC‑AGCGCTCGGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAggg                          >  2:521544/5‑122 (MQ=255)                                                                                                                                    ttggccTGCC‑AGCGCTCGGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAggg                          >  2:521503/5‑122 (MQ=255)                                                                                                                                    gtgtccTGCC‑AGCGCTCGGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAggg                          <  1:521544/118‑1 (MQ=255)                                                                                                                                              gAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAg                                                                             >  2:792480/1‑61 (MQ=255)                                                                                                                                              gAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAg                                                                             <  1:792480/61‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                  GGTTTTCCGGACAGCCAGCTTTACAGCATCGCCATCGACACGCTGGAGGATGCAGCACACTGGCGCGAGCACTGGTATCGCTCTGCTCTGCCCTTCGCCACCGACGGTGTGATCCTGCGCCAGGGCAGTCGGCCACCTGCCGAGCGCT‑GGCAGGCCAAGGCGCCATACTGGATCGCGGCCTGGAAGTACCCCTATGCACAAGCCTTGGCCGAAGTACGCGACGTGCGCTTTCGGGTCGGCCGCACTGGCAGGGTCACGCCCGTTCTTCACGTGCAAC  >  NC_002947/5661156‑5661432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑