Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 566,891 G→A 10.5% L870L (CTG→TTG)  uvrA ← uvrABC excinuclease protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947566,8910GA10.5% 97.8 / 3.7 38L870L (CTG→TTG) uvrAuvrABC excinuclease protein A
Reads supporting (aligned to +/- strand):  ref base G (16/18);  new base A (2/2);  total (18/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

CCGCAGGCAATGATCTGCCCACCCTTGGAACCGCCCTCAGGCCCCAGGTCAACCAGCCAGTCGGCAGTCTTGATCACATCCAGGTTATGCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAGGTCTTGCCGGTATCGCGCTTGGACAGTTCGCGCGACAGCTTTACCCGCTGCGCCTCACCGCCGGACAACGTGGTCGCTGATTGC  >  NC_002947/566746‑567030
                                                                                                                                                 |                                                                                                                                           
ccGCAGGCAATGATCTGCCCACCCTTGGAACCGCCCTCAGGCCCCAGGTCAACCAGCCAGTCGGCAGTCTTGATCACATCCAGGTTATGCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGc                                                                                                                                         >  2:194517/1‑150 (MQ=255)
ccGCAGGAAATGATCTGCCCACCCTTGGAACCGCCCTCAGGCCCCAGGTCAACCAGCCAGTCGGCAGTCTTGATCACATCCAGGTTATGCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGc                                                                                                                                         <  1:593108/150‑1 (MQ=255)
 cGCAGGCAATGATCTGCCCACCCTTGGAACCGCCCTCAGGCCCCAGGTAAACCAGCCAGTCGGCAGTCTTGATTACATCCAGGTTATGCTCGATCCCGCCCCCGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCt                                                                                                                                        <  1:259024/150‑1 (MQ=255)
      gCAATGATCTGCCCACCCTTGGAACCGCCCTCAGGCCCCAGGTCAACCAGCCAGTCGGCAGTCTTGATCACATCCAGGTTATGCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAACTGCTGGacg                                                                                                                                   <  2:706554/150‑3 (MQ=255)
        aaTGATCTGCCCACCCTTGGAACCGCCCTCAGGCCCCAGGTCAACCAGCCGGTCGGCAGTCTTGATCACATCCAGGTTATGCTCGATCACCACCACGGTATGGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATc                                                                                                                                 >  1:446218/1‑150 (MQ=255)
        aaTGATCTGCCCACCCTTGGAACCGCCCTCAGGCCCCAGGTCAACCAGCCAGTCGGCAGTCTTGATCACATCCAGGTTATGCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATc                                                                                                                                 >  2:340208/1‑150 (MQ=255)
                   cACCCTTGGAACCGCCCTCAGGCCCCAGGTCAACCAGCCAGTCGGCAGTCTTGATCACATCCAGGTTATGCTCGATCACCACCACGGTATTGGCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCa                                                                                                                      >  1:8359/1‑150 (MQ=255)
                    aCCCTTGGAACCGCCCTCAGGCCCCAGGTCAACCAGCCAGTCGGCAGTCTTGATCACATCCAGGTTATGCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAg                                                                                                                     <  2:597728/150‑1 (MQ=255)
                    aCCCTTGGAACCGCCCTCAGGCCCCAGGTCAACCAGCCAGTCGGCAGTCTTGATCACATCCAGGTTATGCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAACTGCTGGatgtat                                                                                                                                >  2:553229/1‑135 (MQ=255)
                    aCCCTTGGAACCGCCCTCAGGCCCCAGGTCAACCAGCCAGTCGGCAGTCTTGATCACATCCAGGTTATGCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAACTGCTGGacgtat                                                                                                                                <  1:553229/139‑6 (MQ=255)
                        ttGGAACCGCCCTCAGGCCCCAGGTCAACCAGCCAGTCGGCAGTCTTGATCACATCCAGGTTATGCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCg                                                                                                                 >  2:101367/1‑150 (MQ=255)
                          ggAACCGCCCTCAGGCCCCAGGTCAACCAGCCAGTCGGCAGTCTTGATCACATCCAGGTTATGCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCggt                                                                                                               <  2:320475/150‑1 (MQ=255)
                                gCCCTCAGGCCCCAGGTCAACCAGCCAGTCGGCAGTCTTGATCACATCCAGGTTATGCTCGATCCACACCACGTGATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAgg                                                                                                         <  1:96342/150‑1 (MQ=255)
                                        gCCCCAGGTCAACCAGCCGGTCGGCAGTCTTGATCACATCCAGGTTATGCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGa                                                                                                 <  2:446218/150‑1 (MQ=255)
                                         ccccAGGTCAACCAGCCAGTCGGCAGTCTTGATCACATCCAGGTTATGCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAg                                                                                                >  2:111965/1‑150 (MQ=255)
                                          cccAGGGCCAGCACCCAGTTGCCAGTCTTGATCACAGCCAGGTTATGGTCGATCACCACCACGGTATTGCCGGGGCCGCGCAGGCGAGGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGGTCGTCGAgg                                                                                               <  2:128111/150‑1 (MQ=255)
                                               gTCAACCAGCCAGTCGGCAGTCTTGATCACATCCAGGTTATGCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTa                                                                                          >  2:237107/1‑150 (MQ=255)
                                                   aCCAGCCAGTCGGCAGTCTTGATCACATCCAGGTTATGCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAg                                                                                      <  1:237107/150‑1 (MQ=255)
                                                     cagccagTCGGCAGTCTTGATCACATCCAGGTTATGCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTAAAAGGt                                                                                    >  1:36782/1‑150 (MQ=255)
                                                                     ttGATCACATCCAGGTTATGCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCTATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAGGTCTTGCCGGTATCgcgc                                                                    >  2:75752/1‑150 (MQ=255)
                                                                      tGATCACATCCAGGTTATGCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAGGTCTTGCCGGTATCGCGCt                                                                   >  1:723865/1‑150 (MQ=255)
                                                                        aTCACATCCAGGTTATGCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAGGTCTTGCCGGTATCGCGCTTg                                                                 <  1:111965/150‑1 (MQ=255)
                                                                             aTCCAGGTTATGCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAGGTCTTGCCGGTATCgcgc                                                                    <  1:107098/142‑1 (MQ=255)
                                                                             aTCCAGGTTATGCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAGGTCTTGCCGGTATCgcgc                                                                    >  2:107098/1‑142 (MQ=255)
                                                                                        gCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAGGTCTTGCCGGTATCGCGCtt                                                                  >  2:168997/1‑133 (MQ=255)
                                                                                        gCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAGGTCTTGCCGGTATCGCGCtt                                                                  <  1:168997/133‑1 (MQ=255)
                                                                                          tCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAGGTCTTGCCGGTATCGCGCTTGGACAGTTCGCGCGACAGc                                               <  2:723865/150‑1 (MQ=255)
                                                                                                   accacGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAGGTCTTGCCGGTATCGCGCTTGGACAGTTCGCGCGACAGCTGTACCCGc                                      >  1:632520/1‑150 (MQ=255)
                                                                                                     cacGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAGGTCTTGCCGGTATCGCGCTTGGa                                                               >  1:559014/1‑123 (MQ=255)
                                                                                                     cacGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAGGTCTTGCCGGTATCGCGCTTGGa                                                               <  2:559014/123‑1 (MQ=255)
                                                                                                      acGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAGGTCTTGCCGGTATCGCGCTTGGACAGTTCGCGCGACAGCTTTACCCGCTgc                                   <  1:194517/150‑1 (MQ=255)
                                                                                                                    gTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAGGTCTTGCCGGTATCGCGCTTGGACAGTTCGCGCGACAGCTTTACCCGCTGCGCCTCACCGCCGGa                     >  1:522059/1‑150 (MQ=255)
                                                                                                                    gTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAGGTCTTGCCGGTATCGCGCTTGGACAGTTCGCGCGACAGCTTTACCCGCTGCGCCTCACCGCCGGa                     <  1:101367/150‑1 (MQ=255)
                                                                                                                              cGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAGGTCTTGCCGGTATCGCGCTTGGACAGTTCGCGCGACAGCTTTACCCGCTGCGCCTCACCGCCGGACAACGTGGTc           <  2:522059/150‑1 (MQ=255)
                                                                                                                              cGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAGGTCTTGCCGCTATCGCGCTTACACAGTTAGCGTGACGGATGTACCCGCGGTGCCGTAGCGCGGGACAACGTGGTc           <  2:8359/150‑1 (MQ=255)
                                                                                                                                    aaTACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTtc                                                                                                      >  1:434541/1‑53 (MQ=255)
                                                                                                                                    aaTACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTtc                                                                                                      <  2:434541/53‑1 (MQ=255)
                                                                                                                                       atatCCAGCAACTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAGGTCTTGCCGGTATCGCGCTTGGACAGTTCGCGCGACAGCTTTACCCGCTGCGCCTCACCGCCGGACAACGTGGTCGCTGATTGc  >  2:467651/4‑150 (MQ=255)
                                                                                                                                            cagcagTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAGGTCTTGCCGGTATCGCGCTTg                                                                 >  1:164436/1‑82 (MQ=255)
                                                                                                                                            cagcagTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAGGTCTTGCCGGTATCGCGCTTg                                                                 <  2:164436/82‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                           
CCGCAGGCAATGATCTGCCCACCCTTGGAACCGCCCTCAGGCCCCAGGTCAACCAGCCAGTCGGCAGTCTTGATCACATCCAGGTTATGCTCGATCACCACCACGGTATTGCCGTGGTCGCGCAGGCGATGCAATACGTCCAGCAGTTGCTGGATATCGGCAAAGTGCAGGCCGGTGGTAGGTTCGTCGAGGATGTACAAGGTCTTGCCGGTATCGCGCTTGGACAGTTCGCGCGACAGCTTTACCCGCTGCGCCTCACCGCCGGACAACGTGGTCGCTGATTGC  >  NC_002947/566746‑567030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑