| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 571,620 | G→A | 14.7% | intergenic (+43/+48) | ssb → / ← PP_0486 | single‑stranded DNA‑binding protein/GntR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 571,620 | 0 | G | A | 14.7% | 77.6 / 7.1 | 34 | intergenic (+43/+48) | ssb/PP_0486 | single‑stranded DNA‑binding protein/GntR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (16/13); new base A (3/2); total (19/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01 | |||||||||||
GCCCAGCGCCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGCGATGC > NC_002947/571476‑571765 | gCCCAGCGCCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGcc < 2:722124/150‑1 (MQ=255) ccAGCGCCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCt > 1:713149/1‑150 (MQ=255) cgcCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAACTGCCCTGGGt < 1:769184/150‑1 (MQ=255) cGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGttttt < 2:779475/150‑1 (MQ=255) aaCAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTa < 2:151395/150‑1 (MQ=255) ccagcgccGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTAt > 2:113131/1‑150 (MQ=255) cgcAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCAt > 1:343849/1‑150 (MQ=255) aCTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGc > 2:767294/1‑150 (MQ=255) ttCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGa > 2:288480/1‑150 (MQ=255) tCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGaa < 1:113131/150‑1 (MQ=255) ttCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCgg > 1:322202/1‑130 (MQ=255) ttCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCgg < 2:322202/130‑1 (MQ=255) ttCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCcagcag > 2:494301/1‑150 (MQ=255) atatTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGc < 2:343849/150‑1 (MQ=255) gTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGAAGGCTTGCCAGCTTGGTCa > 2:558505/1‑150 (MQ=255) ttCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAAt > 1:692333/1‑125 (MQ=255) ttCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAAt < 2:692333/125‑1 (MQ=255) cTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAggg < 1:288480/150‑1 (MQ=255) agagCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAgg > 1:507263/1‑143 (MQ=255) agagCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAgg < 2:507263/143‑1 (MQ=255) agagCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGc > 2:535803/1‑150 (MQ=255) gagCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGcc > 1:569624/1‑150 (MQ=255) gagCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGcc < 2:713149/150‑1 (MQ=255) gggCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCtt > 2:427932/1‑150 (MQ=255) cAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCAt > 1:114189/1‑150 (MQ=255) aaaaaCCCAGGGCAACTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATc > 2:251156/3‑150 (MQ=255) aCCCAGGGGAGTTGCCCTGGGTTTTTTTATGGACAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTGCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAggg < 2:40452/150‑1 (MQ=255) aCCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAggg > 2:196544/1‑150 (MQ=255) ccAGGGCAACTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTgg > 2:30943/1‑136 (MQ=255) ccAGGGCAACTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTgg < 1:30943/136‑1 (MQ=255) ccAGGGCAACTGCCCTGGGTTTTTTTATGGGCAGCAACGCGTATCAGTCAGCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGCg > 2:751885/1‑150 (MQ=255) ggCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCTGCTGGGTGCCATCAGGGCGATgc < 2:349842/150‑1 (MQ=255) aGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTgg < 2:604499/129‑1 (MQ=255) aGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTgg > 1:604499/1‑129 (MQ=255) | GCCCAGCGCCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGAACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGCGATGC > NC_002947/571476‑571765 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |