Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 624,515 C→A 9.9% G132V (GGC→GTC)  PP_0537 ← transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947624,5150CA 9.9% 115.9 / 2.1 40G132V (GGC→GTC) PP_0537transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (18/18);  new base A (2/2);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

GTTTACCCTGAGCATGCCGTTTTGCAGCAGGGCATAGGTTTCGCCCTCGACCACCTGGGTCATG‑CCCAGGTCGATGGCCAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGCCCACCGGCGACAGCAAGCCGTTGCGCACTTCGTAGTAGGGCACCTTCAATACCCGGCCACGATGTTCCGACAGCGGGGTAAGCGGGTTGGCG  >  NC_002947/624370‑624664
                                                                                                                                                  |                                                                                                                                                     
gttTACCCTGAGCATGCCGTTTTGCAGCAGGGCATAGGTTTCGCCCTCGACCACCTGGGTCATG‑CCCAGGTCGATGGCCAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGc                                                                                                                                                   >  2:231219/1‑150 (MQ=255)
gttTACCCTGAGCATGCCGTTTTGCAGCAGGGCATAGGTTTCGCCCTCGACCACCTGGGTCATG‑CCCAGGTCGATGGCCAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGc                                                                                                                                                   <  1:236781/150‑1 (MQ=255)
     cccTGAGCATGCCGTTTTGCAGCAGGGCATAGGTTTCGCCCTCGACCACCTGGGTCATG‑CCCAGGTCGATGGCCAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGca                                                                                                                                              >  1:770474/1‑150 (MQ=255)
       cTGAGCATGCCGTTTTGCAGCAGGGCATAGGTTTCGCCCTGGAGAACATGAGTCATG‑CCCAGGTCGATGGCCAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGcaca                                                                                                                                            >  1:138921/1‑150 (MQ=255)
                   ttttGCAGCAGGGCATAGGTTTCGCCCTCGACCACCTGGGTCATG‑CCCAGGTCGATGGCCAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTc                                                                                                                                <  2:138921/150‑1 (MQ=255)
                      tGCAGCAGGGCATAGGTTTCGCCCTCGACCACCTGGGTCATG‑CCCAGGTCGATGGCCAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAgg                                                                                                                             <  1:789676/150‑1 (MQ=255)
                            agGGCATAGGTTTCGCCCTCGACCACCTGGGTCATG‑CCCAGGTCGATGGCCAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTGAAGGGCttgg                                                                                                                       <  2:770474/150‑3 (MQ=255)
                            agGGCATAGGTTTCGCCCTCGACCACCTGGGTCATG‑CCCAGGTCGATGGCCAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGACGGCTTGCACACCCAAGCCCTTCAGGGCtttt                                                                                                                       >  1:286816/1‑150 (MQ=255)
                              ggCATAGGTTTCGCCCTCGACCACCTGGGTCATG‑CCCAGGTCGATGGCCAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGc                                                                                                                     <  1:68181/150‑1 (MQ=255)
                                cATAGGTTTCGCCCTCGACCACCTGGGTCATG‑CCCAGGTCGATGGCCAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGACGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCg                                                                                                                   >  1:461103/1‑150 (MQ=255)
                                      tttCGCCCTCGACCACCTGGGTCATG‑CCCAGGTCGATGGCCAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGACGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAgc                                                                                                             <  2:461103/150‑1 (MQ=255)
                                      tttCGCCCTCCACCACCTGGGTCATG‑CCAAGGTCGATGGCAAGGGTAGCTTGCAGCGGAATCAGCGAGAGAATGTTGCTGGCCGGCATTACCAGGCAGATTTCGTCGCCGGCTTGCACACCCAAGCCCTTCAGGGCGTTTGCCGGCAAgc                                                                                                             >  2:179592/1‑150 (MQ=255)
                                               catcgaccTGGG‑CATGACCCAGGTCGATGGCCAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAACCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGtgct                                                                                                    >  1:3989/6‑150 (MQ=255)
                                                gACCACCTGGGTCATG‑CCCAGGTCGATGGCCAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGtgctt                                                                                                   >  1:241066/1‑150 (MQ=255)
                                                   caccTGGGTCATG‑CCCAGGTCGATGGCCAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGtgcttgct                                                                                                <  2:703609/150‑1 (MQ=255)
                                                    accTGGG‑CATGACCCAGGTCGATGGCCAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTtgctg                                                                                               >  1:529869/1‑150 (MQ=255)
                                                      cTGGGTCATG‑CCCAGGTCGATGGCCAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGcc                                                                                             >  1:600544/1‑150 (MQ=255)
                                                                  ccAGGTCGATGGCCAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGCCCACCGGCGACa                                                                                  >  2:249586/1‑150 (MQ=255)
                                                                  ccAGGTCGATGGCCAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGCCAACCGGCGACa                                                                                  >  2:283281/1‑150 (MQ=255)
                                                                              ccAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGCCCACCGGCGACAGCAAGCCGTTgc                                                                      <  2:447038/150‑1 (MQ=255)
                                                                               cAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGCCCACCGGCGACAGCAAGCCGTTgcg                                                                     >  1:466020/1‑150 (MQ=255)
                                                                               cAGGGTAGCTTACAGCGGCATCAGCGGTAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAAGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGCCCACCGGCGACAGCAAGCCGTTgcg                                                                     >  2:647237/1‑150 (MQ=255)
                                                                                     aGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGCCCACCGGCGACAGCAAGCCGTTGCGCACTTc                                                               <  2:600544/150‑1 (MQ=255)
                                                                                            aGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGCCCACCGGCGACAGCAAGCCGTTGCGCACTTCGtagtag                                                        <  1:249586/150‑1 (MQ=255)
                                                                                             gCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGcaca                                                                                                                                            <  2:190300/65‑1 (MQ=255)
                                                                                             gCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGcaca                                                                                                                                            >  1:190300/1‑65 (MQ=255)
                                                                                                            gCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACCCCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGCCCACCGGCGACAGCAAGCCGTTGCGCACTTCGTAGTAGGGCACCTTCAATAccc                                        <  1:698356/150‑1 (MQ=255)
                                                                                                            gCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGCCCACCGGCGACAGCAAGCCGTTGCGCACTTCGTAGTAGGGCACCTTCAATAccc                                        <  2:466020/150‑1 (MQ=255)
                                                                                                                 ttGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGCCCACCGGCGACAGCAAGCCGTTGCGCACTTCGTAGTAGGGCACCTGCAATACCCGGCCa                                   <  2:529869/150‑1 (MQ=255)
                                                                                                                     tGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGCCCACCGGCGACAGCAAGCCGTTGCGCACTTCGTAGTAGGGCACCTTCAATAccc                                        <  2:219118/141‑1 (MQ=255)
                                                                                                                     tGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGCCCACCGGCGACAGCAAGCCGTTGCGCACTTCGTAGTAGGGCACCTTCAATAccc                                        >  1:219118/1‑141 (MQ=255)
                                                                                                                     tGGCCGGCATTGCCAGGCAGATTGCGTTGACGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGCCCACCGGCGACAGCAAGCCGTTGCGCACTTCGTAGTAGGGCACCTTCAATACCCGGCCACGAt                               <  2:286816/150‑1 (MQ=255)
                                                                                                                        ccGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGCCCACCGGCGACAGCAAGCCGTTGCGCACTTCGTAGTAGGGCACCTTCAATACCCGGCCACGATGtt                            >  2:106383/1‑150 (MQ=255)
                                                                                                                           gCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGCCCACCGGCGACAGCAAGCCGTTGCGCACTTCGTAGTAGGGCACCTTCAATACCCGGCCACGATGTTCCg                         >  2:664/1‑150 (MQ=255)
                                                                                                                                  cAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGCCCACCGGCGACAGCAAGCCGTTGCGCACTTCGTAGTAGGGCACCTTCAATACCCGGCCACGATGTTCCGACAGCgg                  <  1:231219/150‑1 (MQ=255)
                                                                                                                                  cAGGAAGATTGCGTTGCCGGCTTGCACACCCACGCCCTTCAGGGCTTTTGCCGGTCAACGCAGGTGCTTGCTGCCCACCGGCGACAGCAAGCCGTTGCGCACTTCGTAGTAGGGCACCTTCAATACCCGGCCACGATTTTCCGACAGCgg                  <  1:131365/150‑1 (MQ=255)
                                                                                                                                     gCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTGAAGGGCttg                                                                                                                        >  2:387622/1‑44 (MQ=255)
                                                                                                                                     gCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTGAAGGGCttg                                                                                                                        <  1:387622/45‑2 (MQ=255)
                                                                                                                                         aTTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGCCCACCGGCGACAGCAAGCCGTTGCGCACTTCGTAGTAGGGCACCTTCAATACCCGGCCACGATGTTCCGACAGCGGGGTAAGc           >  1:113307/1‑150 (MQ=255)
                                                                                                                                             cGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGCCCACCGGCGACAGCAAGCCGTTGCGCACTTCGTAGTAGGGCACCTTCAATACCCGGCCACGATGTTCCGACAGCGGGGTAAGCGGGt       <  2:241066/150‑1 (MQ=255)
                                                                                                                                                  ccGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGCCCACCGGCGACAGCAAGCCGTTGCGCACTTCGTAGTAGGGCACCTTCAATACCCGGCCACGATGTTCCGACAGCGGGGTAAGCGGGTTggcg  >  1:288844/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GTTTACCCTGAGCATGCCGTTTTGCAGCAGGGCATAGGTTTCGCCCTCGACCACCTGGGTCATG‑CCCAGGTCGATGGCCAGGGTAGCTTGCAGCGGAATCAGCGGGAGCATGTTGCTGGCCGGCATTGCCAGGCAGATTGCGTTGCCGGCTTGCACACCCAAGCCCTTCAGGGCTTTTGCCGGCAAGCGCAGGTGCTTGCTGCCCACCGGCGACAGCAAGCCGTTGCGCACTTCGTAGTAGGGCACCTTCAATACCCGGCCACGATGTTCCGACAGCGGGGTAAGCGGGTTGGCG  >  NC_002947/624370‑624664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑