| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 644,144 | G→C | 9.5% | A292A (GCC→GCG) | acoA ← | acetoin:2,6‑dichlorophenolindophenol oxidoreductase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 644,144 | 0 | G | C | 9.5% | 128.7 / 2.8 | 42 | A292A (GCC→GCG) | acoA | acetoin:2,6‑dichlorophenolindophenol oxidoreductase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base G (20/18); new base C (2/2); total (22/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCTTCGAA > NC_002947/643998‑644284 | tttcttGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCg > 1:376011/1‑150 (MQ=255) tGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGAt < 1:562350/150‑1 (MQ=255) ccATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCCGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCgg > 2:212744/1‑140 (MQ=255) ccATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCgg < 1:212744/140‑1 (MQ=255) ccATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCCCGCGTCGag < 1:272682/148‑2 (MQ=255) ccATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCCCGCGTCGag > 2:272682/1‑147 (MQ=255) cATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCCATGGc < 2:177505/150‑1 (MQ=255) ttCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGTGTGCGGTGGCTTGGGATCGGATTTGGCGCGGCAAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGtt < 1:215929/150‑1 (MQ=255) cTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATg > 2:581586/1‑135 (MQ=255) cTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATg < 1:581586/135‑1 (MQ=255) cTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCg > 2:386999/1‑150 (MQ=255) gttgttCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGAGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGc > 1:579720/1‑150 (MQ=255) ttgttCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGcc > 1:791470/1‑150 (MQ=255) ttGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGc > 1:185062/1‑150 (MQ=255) gCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCTGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCgg < 2:777844/150‑1 (MQ=255) tCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGggt > 1:174043/1‑150 (MQ=255) gggTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTc > 2:722826/1‑150 (MQ=255) ggTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCt > 2:64232/1‑150 (MQ=255) ggCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCttgtt < 2:395714/150‑1 (MQ=255) ggCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCttgtt < 2:174043/150‑1 (MQ=255) aCGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGa < 2:228360/150‑1 (MQ=255) gTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGaaa > 1:81379/1‑150 (MQ=255) aGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAAtt < 2:376011/150‑1 (MQ=255) aGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAAtt < 1:386999/150‑1 (MQ=255) gACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTg > 1:423092/1‑150 (MQ=255) tgGCTTGGGATCGGATTTGGCGCGGAGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAg < 2:579720/150‑1 (MQ=255) gCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCa > 1:588650/1‑72 (MQ=255) gCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCa < 2:588650/72‑1 (MQ=255) gCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGt > 1:347766/1‑150 (MQ=255) ggATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGc < 1:668240/109‑1 (MQ=255) ggATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGc > 2:668240/1‑109 (MQ=255) gcgcGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAggg < 1:64232/150‑1 (MQ=255) gcgcGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAggg < 2:423092/150‑1 (MQ=255) gAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCAt < 2:82378/100‑1 (MQ=255) gAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCAt > 1:82378/1‑100 (MQ=255) ttCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAg < 2:554625/131‑1 (MQ=255) ttCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAg > 1:554625/1‑131 (MQ=255) cAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTAGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGcc > 2:461005/1‑150 (MQ=255) gTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCTTc > 2:499653/1‑150 (MQ=255) catCGACGCCCGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGt > 2:117963/3‑105 (MQ=255) catCGACGCCCGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGt < 1:117963/103‑1 (MQ=255) cTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAg > 2:3191/1‑102 (MQ=255) cTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAg < 1:3191/102‑1 (MQ=255) cTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCTTCGaa > 1:144452/1‑150 (MQ=255) | TTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCTTCGAA > NC_002947/643998‑644284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |