| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 735,628 | C→T | 10.9% | intergenic (‑51/+82) | PP_0627 ← / ← PP_0628 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 735,628 | 0 | C | T | 10.9% | 120.2 / 5.8 | 46 | intergenic (‑51/+82) | PP_0627/PP_0628 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (21/20); new base T (2/3); total (23/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
GGCCGGAAGGGCCTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGC > NC_002947/735486‑735773 | ggCCGGAAGGGCCTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCGAACGCTGTAGCGTTTGCGCAgt < 2:474620/150‑2 (MQ=255) cGGAAGGGCCTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGgcgc < 2:455462/150‑1 (MQ=255) gAAGGGCCTGCGCTCGACTTGGTGCTGGGGGGCTCAGCCTGACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCtt < 1:640138/150‑1 (MQ=255) gCCTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAAc > 2:638003/1‑150 (MQ=255) cTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACaa > 2:475121/1‑150 (MQ=255) tGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACaaa < 1:128417/150‑1 (MQ=255) aCTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACaaa < 2:715991/142‑1 (MQ=255) aCTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACaaa > 1:715991/1‑142 (MQ=255) ttGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCtt < 2:184032/132‑1 (MQ=255) ttGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCtt > 1:184032/1‑132 (MQ=255) gTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTc < 2:570918/150‑1 (MQ=255) ggggggCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATcc > 1:604930/1‑150 (MQ=255) tCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACaaa > 2:643261/1‑125 (MQ=255) tCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACaaa < 1:643261/125‑1 (MQ=255) tCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGgcgc > 1:424212/1‑150 (MQ=255) aGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGCCTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAg > 1:347732/1‑150 (MQ=255) tCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACg > 1:458556/1‑150 (MQ=255) aTCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGcc > 1:122610/1‑150 (MQ=255) tCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCa > 1:712291/1‑150 (MQ=255) cGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCAt < 2:424212/150‑1 (MQ=255) gATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCg < 2:604930/150‑1 (MQ=255) gtCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCGATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGc > 2:546052/2‑150 (MQ=255) ccGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCAc < 1:638003/150‑1 (MQ=255) ggTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTgg < 2:581943/150‑1 (MQ=255) ttCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAGATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGCCGCTGCACTGGAt > 2:280852/1‑150 (MQ=255) ggCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCaaa < 1:616417/150‑1 (MQ=255) cGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGa < 2:472830/150‑1 (MQ=255) aTGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCt < 1:546052/150‑1 (MQ=255) ggtggGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTAc < 2:347732/150‑1 (MQ=255) gCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGa > 1:95191/1‑150 (MQ=255) tCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGaa > 1:642103/1‑148 (MQ=255) tCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGaa < 2:642103/148‑1 (MQ=255) ggTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGAtgc > 1:328392/1‑150 (MQ=255) tAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGAtgctg > 2:459536/1‑150 (MQ=255) tttGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGccc > 2:262776/1‑150 (MQ=255) tgACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGc > 2:582207/1‑81 (MQ=255) tgACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGc < 1:582207/81‑1 (MQ=255) aCTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTg > 2:319441/1‑150 (MQ=255) cTGCGCGAACGCTGTAGCGTTTGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGa < 2:436921/114‑1 (MQ=39) cTGCGCGAACGCTGTAGCGTTTGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGa > 1:436921/1‑114 (MQ=39) cTGCGCGAACGCTGTAGCGTTTGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCg > 1:76334/1‑125 (MQ=255) cTGCGCGAACGCTGTAGCGTTTGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCg < 2:76334/125‑1 (MQ=255) aaaCGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCa < 1:319441/150‑1 (MQ=255) aaCGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAg < 1:262776/150‑1 (MQ=255) gCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGCCGAAGCTGCGGCCCAAGGGACCGCGCGGGAAAGcctg > 2:769749/1‑148 (MQ=255) cGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGgc < 1:475121/150‑1 (MQ=255) | GGCCGGAAGGGCCTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGC > NC_002947/735486‑735773 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |