| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 740,479 | C→G | 11.5% | G51R (GGG→CGG) | pilC ← | type IV fimbrial assembly protein PilC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 740,479 | 0 | C | G | 11.5% | 93.9 / 2.3 | 33 | G51R (GGG→CGG) | pilC | type IV fimbrial assembly protein PilC |
| Reads supporting (aligned to +/- strand): ref base C (15/13); new base G (2/2); total (17/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCTGCGCCGCATCGCAACCACTGCGGCCCATCACCTCGAACGCCTGCAGCAGCGGCACGCCGGCCTTGAGCAACGTCGCCAACTGCCGGCTGAACCCCGCCGGGTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCTGCGCCCGGGTGTTTGGCCGCTGACCGGCGCGCCGTTGGCGTCGGTGCCGTGCCAGTGGTAGAGAAGTGAAGA > NC_002947/740334‑740620 | gccTGCGCCGCATCGCAACCACTGCGGCCCATCACCTCGAACGCCTGCAGCAGCGGCACGCCGGCCTTGAGCAACGTCGCCAACTGCCGGCTGAACCCCGCCGGGTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCCGCTg < 1:630267/150‑1 (MQ=255) ccgcATCGCAACCACTGCGGCCCATCACCTCGAACGCCTGCAGCAGCGGCACGCCGGCCTTGAGCAACGTCGCCAACTGCCGGCTGAACCCCGCCGGGTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCGGGCTggcgttca < 2:611718/150‑6 (MQ=255) ccATCACCTCGAACGCCTGCAGCAGCGGCACGCCGGCCTTGAGCAACGTCGCCAACTGCCGGCTGAACCCCGCCGGGTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGc > 2:28494/1‑150 (MQ=255) cTCGAACGCCTGCAGCAGCGGCACGCCGGCCTTGAGCAACGTCGCCAACTGCCGGCTGAACCCCGCCGGGTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCg > 2:314273/1‑150 (MQ=255) cTGCAGCAGCGGCACGCCGGCCTTGAGCAACGTCGCCAACTGCCGGCTGAACCCCGCCGGGTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGccc > 2:750683/1‑150 (MQ=255) cTGCAGCAGCGGCACGCCGGCCTTGAGCAACGTCGCCAACTGCCGGCTGAACCCCGCCGGGTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGccc > 1:267679/1‑150 (MQ=255) ccTTGAGCAACGTCGCCAACTGCCGGCTGAACCCCGCCGGGTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGc < 1:167173/150‑1 (MQ=255) cTTGAGCAACGTCGCCAACTGCCGGCTGAACCCCGCCGGGTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCt > 2:482038/1‑150 (MQ=255) aGCAACGTCGCCAACTGCCGGCTGAACCCCGCCGGGTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCTgcgc > 2:600756/1‑150 (MQ=255) aaCGTCGCCAACTGCCGGCTGAACCCCGCCGGGTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCGGGCTggcgttc < 1:758519/84‑5 (MQ=255) aaCGTCGCCAACTGCCGGCTGAACCCCGCCGGGTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCGGGCTggcgttc > 2:758519/1‑80 (MQ=255) cGTCGCCAACTGCCGGCTGAACCCCGCCGGGTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCTGCGCCCggg > 2:787689/1‑150 (MQ=255) ccAACTGCCGGCTGAACCCCGCCGGGTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCTGCGCCCGGGTGttt < 1:28494/150‑1 (MQ=255) aaCTGCCGGCTGAACCCCGCCGGGTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCTGCGCCCGGGTGTTTgg < 1:151843/150‑1 (MQ=255) gCTGAACCCCGCCGGGTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCTGCGCCCGGGTGTTTGGCCGCTGAc < 1:750683/150‑1 (MQ=255) tGAACCCCGCCGGGTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCTGCGCCCGGGTGTTTGGCCGCTGACCg > 2:101317/1‑150 (MQ=255) ccccGCCGGGTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCGGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCTGGGCCCGGGTGTTTGGCCGCTGACAGgcgg > 1:305754/1‑149 (MQ=255) ccgccgGGTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATACGCCGGGCTGCGCCCGGGTGGTTGGCCGCTGACCGgcgcgc > 2:298139/1‑150 (MQ=255) gggTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCACGGCGCGCACATAAGCCGGGCTGCGCCCGGGTGTTTGGCCGCTGACCGGCGCGCCGTTg < 1:101317/150‑1 (MQ=255) gTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCTGCGCCCGGGTGTTTGGCCGCTGACCGGCGCGCCGTTGGc < 1:147772/150‑1 (MQ=255) tCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCAGGCTGCGCCCGGGTGTTTGGCCGCTGACCGGCGCGCCGTTGGCg > 2:575547/1‑150 (MQ=255) aGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCTGCGCCCgg > 1:640539/1‑116 (MQ=255) aGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCTGCGCCCgg < 2:640539/116‑1 (MQ=255) cTTTGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCTGCGCCCGGGTGTTTGGCCGCTGACCGGCGCGCCGTTGGCGTCGGTGCCGTGCCAGTg > 2:433573/1‑150 (MQ=255) tttGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCTGCGCCCGGGTGTTTGGc < 1:68816/109‑1 (MQ=255) tttGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCTGCGCCCGGGTGTTTGGc > 2:68816/1‑109 (MQ=255) tttGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCTGCGCCCGGGTGTTTGGCCGCTGACCGGCGCGCCGTTGGCGTCGGTGCCGTGCCAGTgg < 1:600756/150‑1 (MQ=255) gCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCTGCGCCCGGGTGTTTGGCCGCTGACCGGCGCGCCGTTGGCGTCGGTGCCGTGCCAGTGGTaga > 2:587646/1‑150 (MQ=255) aGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCTGCGCCCGGGTGTTTGGCCGCTGACCGGCGCGCCGTTGGCGTCGGTGCTGTTGCTGTGGTAGAGaa < 2:793568/150‑1 (MQ=255) aaCGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCTGCGCCCgg > 2:499651/1‑88 (MQ=255) aaCGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCTGCGCCCgg < 1:499651/88‑1 (MQ=255) aaCGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCTGCGCCCGGGTGTTTGGCCGCTGACCGGCGCGCCGTTGGCGTCGGTGCCGTGCCAGTGGTAGAGAAGTGaa < 1:314273/150‑1 (MQ=255) cGCCAGCCAGCTGGCCGCGCGAAAACTCGCCACCGTGATGCCCTAGCGAATCAGCCCGGCGCGCAGATAAGCTGGGCTGCGCCCGGGTGTTTGGCCGCTGACCGGCGCGCCGTTGGCGTCGGTGCCGTGCCAGTGGTAGAGAAGTGAAGa < 2:151471/150‑1 (MQ=255) | GCCTGCGCCGCATCGCAACCACTGCGGCCCATCACCTCGAACGCCTGCAGCAGCGGCACGCCGGCCTTGAGCAACGTCGCCAACTGCCGGCTGAACCCCGCCGGGTCAGCCTTTTCGCGCCGCTTTGGCAAGCTGAACGCCAGCCCGCTGGCCGGCCGCAAACTCGCCACCGTGATGCCCTGGCGAATCAGCCCGGCGCGCACATAAGCCGGGCTGCGCCCGGGTGTTTGGCCGCTGACCGGCGCGCCGTTGGCGTCGGTGCCGTGCCAGTGGTAGAGAAGTGAAGA > NC_002947/740334‑740620 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |