Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 105,000 G→A 15.2% intergenic (+100/‑207) PP_0099 → / → cynT hypothetical protein/carbonic anhydrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947105,0000GA15.2% 49.6 / 4.4 27intergenic (+100/‑207)PP_0099/cynThypothetical protein/carbonic anhydrase
Reads supporting (aligned to +/- strand):  ref base G (10/12);  new base A (2/2);  total (13/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01

TTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGAATTAAGTTTCAGTTAAGTTTTCCCCGCTAGCGTAGGTAACAAGTCCACACCGACTGTGATCTATGGCCCGTGCGTGTCATCTAA  >  NC_002947/104865‑105138
                                                                                                                                       |                                                                                                                                          
ttttGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCAACCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTc                                                                                                                              >  1:217467/1‑150 (MQ=255)
       cACCGGGTGGGCAGATGGCGCTGTTATGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCtt                                                                                                                       >  1:455533/1‑150 (MQ=255)
                                           cccaccccCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCGGTTCTAGAGCCTTCGAAtt                                                                                   >  2:523321/1‑150 (MQ=255)
                                           cccaccccCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGAAtt                                                                                   <  2:217467/150‑1 (MQ=255)
                                             caccccCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCTAATTCCCGCCTTTTTCATCTGGATCGCTCGTTATAGAGCCTTCGAATTaa                                                                                 >  2:154000/1‑150 (MQ=255)
                                               ccccccACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGAATTAAGt                                                                               >  1:701109/1‑150 (MQ=255)
                                                   ccACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGAATTAAGTTTCa                                                                           <  1:523321/150‑1 (MQ=255)
                                                      caGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAg                                                                                                                                 >  2:494149/1‑93 (MQ=255)
                                                      caGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAg                                                                                                                                 <  1:494149/93‑1 (MQ=255)
                                                             cTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGAATTAAGTTTCAGTTAAGtttt                                                                 >  2:372102/1‑150 (MQ=255)
                                                               gcgcATTGCTCAAAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGAATTAAGTTTCAGTTAAGTTTTcc                                                               <  1:43406/150‑1 (MQ=255)
                                                                     tGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGAATTAAGTTTCAGTTAAGTTTTCCCCGCTa                                                         <  2:701109/150‑1 (MQ=255)
                                                                       cTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTACTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGAATTAAGTTTCAGTTAAGTTTTCCCCGCTAGc                                                       >  2:558934/1‑150 (MQ=255)
                                                                       cTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTACTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGAATTAAGTTTCAGTTAAGTTTTCCCCGCCAGc                                                       >  2:558781/1‑150 (MQ=255)
                                                                        tCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGAATTAAGttt                                                                             <  1:323199/127‑1 (MQ=255)
                                                                        tCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGAATTAAGttt                                                                             >  2:323199/1‑127 (MQ=255)
                                                                            aaCGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGAATTAAg                                                                                <  2:202729/120‑1 (MQ=255)
                                                                            aaCGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGAATTAAg                                                                                >  1:202729/1‑120 (MQ=255)
                                                                                  aCTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGACTTAACTTTCAGTTAAGTTTTCCCCGCTAGCGTAGGTAACaa                                            <  1:154000/150‑1 (MQ=255)
                                                                                           aacCAATAATTGCGCTAATACATATTGGCAACAAATGGATTACTTCGATTATCCAGTTCCCGCCTTTTGCGTCGGGCACGCAAGGCGAAGAGTCTTTGAATTAAGTTTCAGTTAAGTTTTCCCCGCTAGCGTAGGTAATAATACCACAcc                                   >  1:258373/1‑150 (MQ=255)
                                                                                                 taatTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGAATTAAGTTTCAGTTAAGTTTTCCCCGCTAGCGTAGGTAACAAGTCCACACCGACtgt                             <  1:797670/150‑1 (MQ=255)
                                                                                                        gcTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGAATTAAGTTTCAGTTAAGTTTTCCCCGCTAGCGTAGGTAACAAGTCCACACCGACTGTGATCTAt                      <  1:402504/150‑1 (MQ=255)
                                                                                                        gcTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGAATTAAGTTTCAGTTAAGTTTTCCCCGCTAGCGTAGGTAACAAGTCCACACCGACTGTGATCTAt                      <  2:455533/150‑1 (MQ=255)
                                                                                                          tAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGAATTAAGTTTCAGTTAAGTTTTCCCCGCTAGCGTAGGTAACAAGTCCACACCGACTGTGATCTATgg                    >  2:276481/1‑150 (MQ=255)
                                                                                                                      gCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGAATTAAGTTTCAGTTAAGTTTTCCCCGCTAGCGTAGGTAACAAGTCCACACCGACTGTGATCTATGGCCCGTGCGTGTc        <  1:276481/150‑1 (MQ=255)
                                                                                                                            gATGTTTTTTTACTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGAATTAAGTTTCAGTTAAGTTTTCCCCGCTAGCGTAGGTAACAAGTCCACACCGACTGTGATCTATGGCCCGTGCGTGTCATCTaa  <  1:558934/150‑1 (MQ=255)
                                                                                                                            gATGTTTTTTTACTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGAATTAAGTTTCAGTTAAGTTTTCCCCGCCAGCGTAGGTAACAAGTCCACACCGACTGTGATCTATGGCCCGTGCGTGTCATCTaa  <  1:558781/150‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                          
TTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTTCATCTGGAACGCCCGTTCTAGAGCCTTCGAATTAAGTTTCAGTTAAGTTTTCCCCGCTAGCGTAGGTAACAAGTCCACACCGACTGTGATCTATGGCCCGTGCGTGTCATCTAA  >  NC_002947/104865‑105138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑