| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 743,628 | A→G | 11.1% | I237I (ATT→ATC) | PP_0636 ← | cold shock DNA‑binding domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 743,628 | 0 | A | G | 11.1% | 95.2 / 3.4 | 36 | I237I (ATT→ATC) | PP_0636 | cold shock DNA‑binding domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (12/20); new base G (2/2); total (14/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
CCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCGTGTTTGAATGCTCCGGGTGGTACCAGGCTGGTTACGCCGGTATCCCAACCTGCGGGCTGGGCGTTTTCATTTTCGATGCGAGCTAA > NC_002947/743483‑743774 | ccGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATgg < 1:215063/150‑1 (MQ=255) gggAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGAATCAAGCTAGCTTGACGGTCACGCTAAGCCGAGTGAGGCATTGGATCCTGCTGGCGACGCATTTGCTTAGCTAAGTCCACAATGCCGa > 2:340494/1‑150 (MQ=255) ctactTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCa > 2:705337/1‑150 (MQ=255) gtgGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAAtgtg < 2:589257/150‑1 (MQ=255) tgGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTgg < 1:50618/150‑1 (MQ=255) tGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGTTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATTGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCa < 2:65765/150‑1 (MQ=255) tGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGGTGGGCGGTCACCCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCa < 2:188840/150‑1 (MQ=255) aGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGa < 1:436837/150‑1 (MQ=255) ggAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCa < 2:780791/150‑1 (MQ=255) gCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGaa < 1:753044/150‑1 (MQ=255) ggCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCgtgt < 2:397198/150‑1 (MQ=255) gCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACGATGGCGAAGCCCAGGATTTTCATGa > 1:408829/1‑111 (MQ=255) gCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACGATGGCGAAGCCCAGGATTTTCATGa > 1:408993/1‑111 (MQ=255) gCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACGATGGCGAAGCCCAGGATTTTCATGa < 2:408993/111‑1 (MQ=255) gCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACGATGGCGAAGCCCAGGATTTTCATGa < 2:408829/111‑1 (MQ=255) ttCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCGTGTTTGAAt > 1:780354/1‑150 (MQ=255) agctagctTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCgtgt < 1:118987/140‑1 (MQ=255) agctagctTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCgtgt > 2:118987/1‑140 (MQ=255) ctTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCGTGTTTGAATGCTCCGggtg < 1:69137/150‑1 (MQ=255) ttGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCGTGTTTGAATGCTCCGggt > 2:504155/1‑148 (MQ=255) ttGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCGTGTTTGAATGCTCCGggt < 1:504155/148‑1 (MQ=255) gAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCAt > 2:505754/1‑74 (MQ=255) gAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCAt < 1:505754/74‑1 (MQ=255) aGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCGTGTTTGAATGCTCCGGGTGGTACCAGGCTGGTTACGCCgg > 1:705542/1‑150 (MQ=255) agAGGAATTCGCGCCTCCTAGCGACGCAGCTGCTTAGCTAACGCAAGAATGCGGGGGCCGAGGATTTTCATGGATGTGGCCGTCGAGCTCAGGAGCAGAATAGCTCCGTGTGTGAATGCGCCGGGTGGGACCAGGCTGGTTACGCCGGTa > 2:311679/2‑150 (MQ=255) ggCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCGTGTTTGAATGCTCCGGGTGGTACCAGGCTGGTTACGCCGGTATcc < 2:705542/150‑1 (MQ=255) ttCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCGTGTTTGAATGCTCCGGGTGGTACCAGGCTGGTTACGCCGGTATCCCAAc < 1:720382/150‑1 (MQ=255) aTCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCGTGTTTGAATGCTCCGGGTGGTACCAGGCTGGTTACGCCGGTATCCCAACCTGc > 2:138462/1‑150 (MQ=255) cTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCACAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCGTGTTTGAATGCTCCGTGTGGTACCAGGCTGGTTACGCCGGTATCCCAACCTGCGGGCTg < 1:576036/150‑1 (MQ=255) aCGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCGTGTTTGAATGCTCCGGGTGGTACCAGGCTGGTTACGcc > 2:270798/1‑123 (MQ=255) aCGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCGTGTTTGAATGCTCCGGGTGGTACCAGGCTGGTTACGcc < 1:270798/123‑1 (MQ=255) gCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCGTGTTTGAATGCTCCGGGTGGTACCAGGCTGGTTACGCCGGTATCCCAACCTGCGGGCTGGGCGtttt < 2:780354/150‑1 (MQ=255) gCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTc > 1:527391/1‑53 (MQ=255) gCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTc < 2:527391/53‑1 (MQ=255) tAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCGTGTTTGAATGCTCCGGGTGGTACCAGGCTGGTTACGCCGGTATCCCAACCTGCGGGCTGGGCGTTTTCATTTTCGa < 1:705337/150‑1 (MQ=255) ccACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCGTGTTTGAATGCTCCGGGTGGTACCAGGCTGGTTACGCCGGTATCCCAACCTGCGGGCTGGGCGTTTTCATTTTCGATGCGAGCTa > 1:742260/1‑150 (MQ=255) cacaATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCGTGTTTGACTGATGCGGGTGGTACCAAGACGCTTAAGGATCTCGCCATACCCTCAGTTTGGGCGTTTTCATTTTCGATGCGAGCTaa > 1:575296/1‑150 (MQ=255) cacaATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCGTGTTTGAATGCTCCGGGTGGTACCAGGCTGGtt > 2:587451/1‑97 (MQ=255) cacaATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCGTGTTTGAATGCTCCGGGTGGTACCAGGCTGGtt < 1:587451/97‑1 (MQ=255) | CCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATTTGCTTAGCTAAGGCCACAATGGCGAAGCCCAGGATTTTCATGAATGTGGCCGTCCAGCTCAGGAGCAGAATAGCTCCGTGTTTGAATGCTCCGGGTGGTACCAGGCTGGTTACGCCGGTATCCCAACCTGCGGGCTGGGCGTTTTCATTTTCGATGCGAGCTAA > NC_002947/743483‑743774 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |