Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 897,774 C→G 10.3% I401M (ATC→ATG)  PP_0779 → methyl‑accepting chemotaxis transducer/sensory box protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947897,7740CG10.3% 110.4 / 3.3 39I401M (ATC→ATG) PP_0779methyl‑accepting chemotaxis transducer/sensory box protein
Reads supporting (aligned to +/- strand):  ref base C (17/17);  new base G (2/2);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

GCCGCCCGTGCCGGTGAGCAAGGGCGGGGCTTTGCCGTCGTTGCCGACGAAGTGCGCCAACTGGCCTCTCGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAGCGTAATTGTCGAGATCCAGGACGGGGCGAAGCGGGTGGTGGATGTGGTGGGGCAGTTTTCCAGCCGGCTGAGTCTTTGATATGGCTTGGG  >  NC_002947/897628‑897923
                                                                                                                                                  |                                                                                                                                                     
gccgccCGTGCCGGTGAGCAAGGGCGGGGCTTTGCCGTCGTTGCCGACGAAGTGCGCCAACTGGCCTCTCGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGatcatc                                                                                                                                                    <  1:537112/150‑1 (MQ=255)
       gTGCCGGTGAGCAAGGGCGGGGCTTTGCCGTCGTTGCCGACGAAGTGCGCCAACTGGCCTCTCGCACCAGCCCCGCCACCGAAGAACTCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCGGTGTCGATCAGCGACACCa                                                                                                                                             <  2:8551/150‑1 (MQ=255)
       gTGCCGGTGAGCAAGGGCGGGGCTTTGCCGTCGTTGCCGACGAAGTGCGCCAACTGGCCTCTCGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCa                                                                                                                                             <  1:486809/150‑1 (MQ=255)
                  cAAGGGCGGGGCTTTGCCGTCGTTGCCGACGAAGTGCGCCAACTGGCCTCTCGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATGATCGacactg                                                                                                                                             <  1:616749/139‑3 (MQ=255)
                  cAAGGGCGGGGCTTTGCCGTCGTTGCCGACGAAGTGCGCCAACTGGCCTCTCGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATGATCGacactg                                                                                                                                             >  2:616749/1‑137 (MQ=255)
                     gggCGGGGCTTTGCCGTCGTTGCCGACGAAGTGCGCAAACTGGCCTCTCGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGcc                                                                                                                               <  1:41591/150‑1 (MQ=255)
                         ggggCTTTGCCGTCGTTGCCGACGAAGTGCGCCAACTGGCCTCTCGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAAc                                                                                                                           <  2:760518/150‑1 (MQ=255)
                             cTTTGCCGTCGTTGCCGACGAAGTGCGCCAACTGGCCTCTCGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAggg                                                                                                                       >  1:385520/1‑150 (MQ=255)
                             cTTTGCCGTCGTTGCCGACGAAGTGCGCCAACTGGCCTCTCGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAggg                                                                                                                       <  1:89395/150‑1 (MQ=255)
                                  ccGTCGTTGCCGACGAAGTGCGCCAACTGGCCTCTCGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGgctgg                                                                                                                  >  1:727407/1‑150 (MQ=255)
                                  ccGTCGTTGCCGACGAAGTGCGCCAACTGGCCTCTCGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGgctgg                                                                                                                  >  2:684512/1‑150 (MQ=255)
                                    gtcgtTGCCGACGAAGTGCGCCAACTGGCCTCTCGCACCAGCCCCGCCACCGAAGAAATCGCCCGGGGGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCCAGCGCCAAGCCGAACAGGGgctggcg                                                                                                                >  2:283222/1‑150 (MQ=255)
                                            cgacgaAGTGCGCCAACTGGCCTCTCGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGa                                                                                                                                                  <  1:742749/108‑1 (MQ=255)
                                            cgacgaAGTGCGCCAACTGGCCTCTCGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGa                                                                                                                                                  >  2:742749/1‑108 (MQ=255)
                                            cgacgaAGTGCGCCAACTGGCCTCTCGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGa                                                                                                        <  2:727407/150‑1 (MQ=255)
                                             gacgaAGTGCGCCAACTGGCCTCTCGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGAt                                                                                                       >  1:52813/1‑150 (MQ=255)
                                                      cgcCAACTGGCCTCTCGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTGGATCATCGGCACCAACAGTCGCGATGCCGAACAGGGGCTGGCGGTGGCGGATGAGACGTGc                                                                                              >  2:105165/1‑150 (MQ=255)
                                                        ccAACTGGCCTCTCGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAg                                                                                            >  2:489996/1‑150 (MQ=255)
                                                             tGGCCTCTCGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGcc                                                                                                                               <  2:124509/110‑1 (MQ=255)
                                                             tGGCCTCTCGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGcc                                                                                                                               >  1:124509/1‑110 (MQ=255)
                                                                  tctcGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCCTGCTCACCATCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAGCGTAATTGTc                                                                                  >  1:56356/1‑150 (MQ=255)
                                                                  tctcGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAGCGTAATTGTc                                                                                  >  2:766857/1‑150 (MQ=255)
                                                                  tctcGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAGCGTAATTGTc                                                                                  >  2:582870/1‑150 (MQ=255)
                                                                    tcGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGgctggc                                                                                                                 >  2:296449/1‑117 (MQ=255)
                                                                    tcGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGgctggc                                                                                                                 <  1:296449/117‑1 (MQ=255)
                                                                        aCCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAGCGTAATTGTCGAGATc                                                                            >  2:667986/1‑150 (MQ=255)
                                                                                          gaaATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAGCGTAATTGTCGAGATCCAGGACGGGGCGAAGCgg                                                          <  1:489996/150‑1 (MQ=255)
                                                                                                    gggTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAGCGTAATTGTCGAGATCCAGGACGGGGCGAAGCGGGTGGTGGAtg                                                >  1:355428/1‑150 (MQ=255)
                                                                                                         gtACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAGCGTAATTGTCGAGATCCAGGACGGGGCGAAGCGGGTGGTGGATgtggtg                                           <  1:547826/150‑1 (MQ=255)
                                                                                                          gaCAGCAGGACGAACAACTGGCGCAGGAAGCAGTGTCGCTATTCGACACCAGAAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAGCGTAATTGTCGAGATCCAGGACGGGGCGAAGCGGGTGGTGGATGtggtgg                                          <  2:330085/149‑1 (MQ=255)
                                                                                                           aCAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAGCGTAATTGTCGAGATCCAGGACGGGGCGAAGCGGGTGGTGGATGTGGTggg                                         <  1:582870/150‑1 (MQ=255)
                                                                                                            cagcagAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAGCGTAATTGTCGAGATCCAGGACGGGGCGAAGCGGGTGGTGGATGTGGTgggg                                        <  1:766857/150‑1 (MQ=255)
                                                                                                            cagcagAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAGCGTAATTGTCGAGATCCAGGACGGGGCGAAGCGGGTGGTGGATGTGGTgggg                                        <  2:52813/150‑1 (MQ=255)
                                                                                                               cagAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAGCGTAATTGTCGAGATCCAGGACGGGGCGAAGCGGGTGGTGGATGTGGTGGGGCAg                                     <  1:684512/150‑1 (MQ=255)
                                                                                                                         aacTGGCGCAGGCAGAAGGGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAGCGTAATTGTCGAGATCCAGGACGGGGCGAAGCGGGTGGTGGATGTGGTGGGGCAGTTTTCCAGcc                           >  1:786904/1‑150 (MQ=255)
                                                                                                                                         agTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAGCGTAATTGTCGAGATCCAGGACGGGGCGAAGCGGGTGGTGGATGTGGTGAGGCAGTTTTCCAGCCGGCTGAGTCTTTGata           <  1:105165/150‑1 (MQ=255)
                                                                                                                                          gtgtCGATGATCGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAGCGTAATTGTCGAGATCCAGGACGGGGCGAAGCGggtg                                                       >  1:529236/1‑105 (MQ=255)
                                                                                                                                          gtgtCGATGATCGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAGCGTAATTGTCGAGATCCAGGACGGGGCGAAGCGggtg                                                       <  2:529236/105‑1 (MQ=255)
                                                                                                                                          gtgtCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAGCGTAATTGTCGAGATCCAGGACGGGGCGAAGCGGGTGGTGGATGTGGTGGGGCAGTTTTCCAGCCGGCTGAGTCTTTGa             <  2:583278/147‑1 (MQ=255)
                                                                                                                                          gtgtCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAGCGTAATTGTCGAGATCCAGGACGGGGCGAAGCGGGTGGTGGATGTGGTGGGGCAGTTTTCCAGCCGGCTGAGTCTTTGa             >  1:583278/1‑147 (MQ=255)
                                                                                                                                                  catcGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAGCGTAATTGTCGAGATCCAGGACGGGGCGAAGCGGGTGGTGGATGTGGTGGGGCAGTTTTCCAGCCGGCTGAGTCTTTGATATGGCTTggg  <  1:667986/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GCCGCCCGTGCCGGTGAGCAAGGGCGGGGCTTTGCCGTCGTTGCCGACGAAGTGCGCCAACTGGCCTCTCGCACCAGCACCGCCACCGAAGAAATCGCCCGGGTGGTACAGCAGAACGAACAACTGGCGCAGGCAGCAGTGTCGATCATCGACACCAGCAAGCGCCAAGCCGAACAGGGGCTGGCGCTGGCGGATGAGACGGGCAGCGTAATTGTCGAGATCCAGGACGGGGCGAAGCGGGTGGTGGATGTGGTGGGGCAGTTTTCCAGCCGGCTGAGTCTTTGATATGGCTTGGG  >  NC_002947/897628‑897923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑