| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 919,675 | G→C | 10.2% | intergenic (‑143/+48) | PP_5458 ← / ← PP_0800 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 919,675 | 0 | G | C | 10.2% | 136.8 / 5.9 | 46 | intergenic (‑143/+48) | PP_5458/PP_0800 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (22/19); new base C (3/2); total (25/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
ATTTGAAACTACCTTCTTGCGGTGCAAGTGCTAATGAGGACAGGGGTAAAGTTAGTCCTTTCAGTGCACTCGAAACAGAGCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCGGGGTTTCATGGGTTGTGCGCAAACCCAGTGCCTGCAAGCTTGCCGTCAGCGCTTCATCCTGCACATGCAAG > NC_002947/919531‑919822 | aTTTGAAACTACCTTCTTGCGGTGCAAGTGCTAATGAGGACAGGGGTAAAGTTAGTCCTTTCAGTGCACTCGAAACAGAGCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGaaa < 1:756363/150‑1 (MQ=255) aaaCTACCTTCTTGCGGTGCAAGTGCTAATGAGGACAGGGGTAAAGTTAGTCCTTTCAGTGCACTCGAAACAGAGCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGCCGAAAGcccc < 2:313065/150‑1 (MQ=255) aaaCTACCTTCTTGCGGTGCAAGTGATAATGAGGACAGGGGTAAAGTTAGTCCTTTCAGTGCACTCGAAACAGAGCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGCCGAAAGcccc < 1:394909/150‑1 (MQ=255) gCGGTGCAAGTGCTAATGAGGACAGGGGTAAAGTTAGTCCTTTCAGTGCACTCGAAACAGAGCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGcc < 2:310022/150‑1 (MQ=255) tAATGAGGACAGGGGTAAAGTTAATCCTTTCGGTGCACTCGAAACAGAGCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGcgcc > 2:490102/1‑150 (MQ=255) aTGAGGACAGGGGTAAAGTTAGTCCTTTCAGTGAACTCGAAACAGAGCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGAGCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGcgccgc > 1:63601/1‑150 (MQ=255) cAGGGGTAAAGTTAGTCCTTTCAGTGCACTCGAAACAGAGCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCa < 2:141931/150‑1 (MQ=255) aGGGGTAAAGTTAGTCCTTTCAGTGCACTCGAAACAGAGCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAg > 2:437577/1‑150 (MQ=255) ggTAAAGTTAGTCCTTTCAGTGCACTCGAAACAGAGCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCt > 2:635366/1‑150 (MQ=255) gTAAAGTTAGTCCTTTCAGTGCACTCGAAACAGAGCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTa > 1:470352/1‑150 (MQ=255) ttAGTCCTTTCAGTGCACTCGAAACAGAGCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAAcc > 2:605291/1‑150 (MQ=255) aGTCCTTTCAGTGCACTCGAAACAGAGCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGa < 1:635366/150‑1 (MQ=255) tCCTTTCAGTGCACTCGAAACAGAGCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATa > 1:374541/1‑150 (MQ=255) tttCAGTGCACTCGAAACAGAGCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGGAGGCTAGAAACCGATAAAg > 2:524737/1‑150 (MQ=255) gCACTCGAAACAGAGCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGtaat < 2:616075/150‑1 (MQ=255) aCTCGAAACAGAGCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGtaataa > 1:590249/1‑150 (MQ=255) cTCGAAACAGAGCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATaaa < 2:583081/150‑1 (MQ=255) tCGAAACAGAGCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAAc > 1:602445/1‑150 (MQ=255) aaaCAGAGCGAAGTGCGATAAGCACTGTTGCGCGAACGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCgg < 2:63601/150‑1 (MQ=255) agtgCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAg < 2:596429/129‑1 (MQ=255) agagCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAg > 1:596429/1‑132 (MQ=255) gAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCCTGGCAGGCTAGAAACCCGTAAAGGCGTAATAACCCGGGGGTTCATg < 2:232162/150‑1 (MQ=255) tGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCGGGGTTTCATGGGtt > 1:533106/1‑150 (MQ=255) tGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCGGGGTTTCATGGGtt < 1:605291/150‑1 (MQ=255) gATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCGGGGTTTCATGGGTtgtg > 1:90627/1‑150 (MQ=255) aTAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCGGGGTTTCATGGGTTGTgc < 1:12391/150‑1 (MQ=255) aTAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCGGGGTTTCATGGGTTGTgc > 1:340492/1‑150 (MQ=255) ctcAAAAGGTAACGATCAGTGGGTGGAACAGCGGGGGTTTGGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCGGGGTTTCATGGGTTGTGCGCAAACCCAGTGc < 2:564558/148‑1 (MQ=255) aaaaGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCGGGGTTTCATGGGTTGTGCGCAAACCCAGTGccag > 1:564558/1‑148 (MQ=255) tAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCCGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCGGGGTTTCATGGGTTGTGCGCAAACCCAGTGCCTGCAAGCt > 2:507156/1‑150 (MQ=255) gATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGgcgc > 1:425365/1‑65 (MQ=255) gATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGgcgc < 2:425365/65‑1 (MQ=255) gATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCGGGGTTTCATGGGTTGTGCGCAAACCCAGTGCCTGCAAGCTTGcc > 2:190118/1‑150 (MQ=255) cATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCGGGGTTTCATGGGTTGTGCGCAAACCCAGTGCCTGCAAGCTTGCCGTc < 1:437577/150‑1 (MQ=255) aTTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAATGCGTAATAAACCGGGGTTTCATGGTTTGTGCTGAAACCCATTGCCTGCAAGCTTGCCGTCa < 2:8890/150‑1 (MQ=255) gTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCGGGGTTTCATGGGTTGTGCGCAAACCCAGTGCCTGCAAGCTTGCCGTCAGCGCt < 1:190118/150‑1 (MQ=255) gAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCGGGGTTTCATGGGTTGTGCGCAAACCCAGTGCCTGCAAGCTTGCCGTCAGCGCTTCa > 2:576621/1‑150 (MQ=255) acGGGGCTTTCGCCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCGGGGTTTCATGGGTTGTGCGCAAACCCAGTGCCTGCAAGCTTGCCGTCAGCGCTTCATCCTg > 1:290676/1‑150 (MQ=255) acGGGGCTTTCGCCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCGGGGTTTCATGGGTTGTGCGCAAACCCAGTGCCTGCAAGCTTGCCGTCAGCGCTTCATCCTg > 1:290477/1‑150 (MQ=255) gggCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCGGGGTTTCATGGGTTGTGCGCAAACCCAGTGCCTGCAAGCTTGCCGTCAGCGCTTCATCCTGcac > 2:79200/1‑150 (MQ=255) gggCTTTCGCCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCGGGGTTTCATGGGTTGTGCGCAAACCCAGTGCCTGCAAGCTTGCCGTCAGCGCTTCATCCTGcac > 2:734653/1‑150 (MQ=255) ggCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTa > 1:647889/1‑59 (MQ=255) ggCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTa < 2:647889/59‑1 (MQ=255) gCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCGGGGTTTCATGGGTTGTGCGCAAACCCAGTGCCTGCAAGCTTGCCGTCAGCGCTTCATCCTGCACAt < 2:470352/150‑1 (MQ=255) gCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCGGGGTTTCATGGGTTGTGCGCAAACCCAGTGCCTGCAAGCTTGCCGTCAGCGCTTCATCCTGCACAt > 1:757529/1‑150 (MQ=255) cGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCGGGGTTTCATGGGTTGTGCGCAAACCCAGTGCCTGCAAGCTTGCCGTCAGCGCTTCATCCTGCACATGCAAg < 2:590249/150‑1 (MQ=255) | ATTTGAAACTACCTTCTTGCGGTGCAAGTGCTAATGAGGACAGGGGTAAAGTTAGTCCTTTCAGTGCACTCGAAACAGAGCGAAGTGCGATAAGCACTGTTGCGCAAAAGGTAACGATCATTGGGTGGAACAACGGGGCTTTCGGCGAAAGCCCCGTGCTAGGGCGCCTGCACAGGGCGCCGCCTTGGCAGGCTAGAAACCGATAAAGGCGTAATAAACCGGGGTTTCATGGGTTGTGCGCAAACCCAGTGCCTGCAAGCTTGCCGTCAGCGCTTCATCCTGCACATGCAAG > NC_002947/919531‑919822 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |